• Title/Summary/Keyword: Standard positive control

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Development of Reverse Transcriptase Polymerase Chain Reaction Primer Sets and Standard Positive Control Capable of Verifying False Positive for the Detection of Severe acute respiratory syndrome coronavirus 2

  • Cho, Kyu Bong
    • Biomedical Science Letters
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    • v.27 no.4
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    • pp.283-290
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    • 2021
  • Severe acute respiratory syndrome coronavirus (SARS-CoV2) is a major coronavirus that infects humans with human Coronavirus (HuCoV)-229E, HCoV-OC43, HCoV-HKU1, HCoV-NL63, Severe acute respiratory syndrome coronavirus (SARS-CoV) and Middle east respiratory syndrome coronavirus (MERS-CoV). SARS-CoV2 is currently a global pandemic pathogen. In this study, we developed conventional RT-PCR based diagnostic system for the detection of SARS-CoV2 which is relatively inexpensive but has high stability and a wide range of users. Three conventional RT-PCR primer sets capable of forming specific band sizes by targeting the ORF1ab [232 nucleotide (nt)], E (200 nt) and N (288 nt) genes of SARS-CoV2 were developed, respectively, and it were confirmed to be about 10~100 times higher detection sensitivity than the previously reported methods. In addition, a standard positive control that can generate specific amplicons by reacting with the developed RT-PCR primers and verify the false-positiv from contamination of the laboratory was produced. Therefore, the diagnostic system that uses the RT-PCR method is expected to be used to detect SARS-CoV2.

Robust Positive Real Control of Linear Systems with Repeated Scalar Block Parameter Uncertainty (반복된 스칼라 블록 파라미터를 포함한 불확실성을 갖는 선형 시스템의 가인 양실 제어)

  • 이보형;심덕선;이장규
    • Journal of Institute of Control, Robotics and Systems
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    • v.4 no.5
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    • pp.574-578
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    • 1998
  • This paper considers the robust positive real problem for linear systems with linear fractional-type norm-bounded repeated scalar block parameter uncertainty. It is shown that the robust positive real problem can be converted into the standard positive real problem without uncertainty that can be used for the analysis of the given uncertain linear system and the synthesis of a controller that robustly stabilizes and achieves the extended strict positive realness property of the closed-loop transfer function. These results can be also applied to the linear system with general structured uncertainty containing repeated scalar block parameters and are extensions of the previous works that consider only norm-boundedness of the affine unstructured uncertainty.

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Synthesis of Sector-Bounded Control : General Approach of $H{\infty}$ Control and Positive Real Control ($H{\infty}$제어와 양실 제어의 일반형태인 구간영역제어기의 설계)

  • 심덕선
    • Journal of Institute of Control, Robotics and Systems
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    • v.5 no.1
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    • pp.1-10
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    • 1999
  • We consider the problem of synthesizing an internally stabilizing linear time-invariant controller for a linear tune-Invariant plant such that a given closed loop transfer function is strictly sector bounded. We show that the standard $H{\infty}$ control problem and the $\tau$ -positive real control problem are special cases of sector bounded control problem. Necessary and sufficient conditions for the existence of a controller are obtained. The state-space representation for strictly proper controllers are given in terms of solutions to ARIs or AREs.

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Awareness and Performance on Standard Precautions of Hospital-acquired Infection Control in Nursing Students: The Mediating Effect of Internal Health Locus of Control (간호 대학생의 의료관련감염 관리에 대한 표준주의 인지도와 표준주의 수행도: 내적 건강통제위의 매개효과)

  • Yang, Sun-Yi;Lim, Hyo-Nam
    • The Journal of Korean Academic Society of Nursing Education
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    • v.23 no.4
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    • pp.378-388
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    • 2017
  • Purpose: The aim of this study was to identify the status of performance on standard precautions among nursing students and to examine the mediating effects of internal health locus of control on the relationship between awareness and performance on standard precautions of hospital-acquired infection control. Methods: The participants in this study were 134 nursing students. The measurements included a standard precautions awareness and performance scale, and a multidimensional health locus of control scale. Data were analyzed using independent t-test, one-way ANOVA, $Scheff{\acute{e}}$ test, Pearson correlation coefficient, and simple and multiple regression techniques. Mediation analysis was performed by the Baron and Kenny's method and Sobel test. Results: The mean score of standard awareness, standard performance, and internal health locus of control about standard precaution were $174.30{\pm}9.08$; $169.48{\pm}12.04$; and $20.43{\pm}2.82$; respectively. There was a positive correlation between standard awareness and performance (r=.414, p<.001). Also, standard awareness was significantly correlated with internal health locus of control (r=.413, p=.014). Internal health locus of control showed partial mediating effects in the relationship between awareness and performance. Conclusion: The results indicate a need to improve the internal health locus of control of nursing students. Therefore, an internal health locus of control improvement program should improve performance on standard precautions for patients and themselves.

Design of Variable Gain Amplifier with a Gain Slope Controller in Multi-standard System (다중 표준 시스템을 위한 이득 곡선 제어기를 가진 가변이득 증폭기 설계)

  • Choi, Moon-Ho;Lee, Won-Young;Kim, Yeong-Seuk
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.21 no.4
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    • pp.321-328
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    • 2008
  • In this paper, variable gain amplifier(VGA) with a gain slope controller has been proposed and verified by circuit simulations and measurements. The proposed VGA has a gain control, gain slope switch and variable gain range. The input source coupled pair with diode connected load is used for VGA gain stage. The gain slope controller with switch can control VGA gain slope. The proposed VGA is fabricated in $0.18{\mu}m$ CMOS process for multi -standard wireless receiver. The proposed two stage VGA consumes min. 2.0 mW to max. 2.6 mW in gain control range and gives input IP3 of -3.77 dBm and NF of 28.7 dB at 1.8 V power supply under -25 dBm, 1 MHz input. The proposed VGA has 37 dB(-16 dB $\sim$ 21 dB) variable gain range, and 8 dB gain range control per 0.3 V control voltage, and can provide variable gain, positive and negative gain slope control, and gain range control. This VGA characteristics provide design flexibility in multi-standard wireless receiver.

The Effects of Organizational Culture for Infection Control and Self-Efficacy on Compliance with Standard Precautions of Emergency Room Nurses (응급실 간호사의 감염관리 조직문화와 자기효능감이 표준주의 지침 수행에 미치는 영향)

  • Kim, Hyunhee;Park, Hyung-Ran
    • Journal of Korean Biological Nursing Science
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    • v.21 no.1
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    • pp.46-53
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    • 2019
  • Purpose: This study was conducted to identify the effects of organizational culture for the infection control and self-efficacy on compliance with standard precautions of emergency room nurses. Methods: This cross-sectional descriptive study was conducted from 1st July to 3rd August, 2017. The participants were 200 nurses working in emergency rooms. The data was collected using structured self-report questionnaires. Results: The average scores for the organizational culture for infection control, self-efficacy and compliance with standard precautions were 5.54, 3.65, and 4.31, respectively. The organizational culture for infection control (r = .59, p< .001) and self-efficacy (r= .28, p< .001) were found to have a positive, meaningful correlation with compliance with the standard precautions. Multiple regression analysis showed that compliance with standard precautions was significantly affected by the organizational culture for infection control (${\beta}=0.55$, p< .001) and self-efficacy (${\beta}=0.13$, p= .033). Conclusion: The results for this study indicate that the organizational culture for infection control and self-efficacy were highly influential factors affecting compliance with standard precautions of emergency room nurses. Therefore, in order to improve the nurses' use of the standard precautions, it was necessary to develop and evaluate a program that considers the environmental factors and self-efficacy.

$\bar{X}$ control charts of automcorrelated process using threshold bootstrap method (분계점 붓스트랩 방법을 이용한 자기상관을 갖는 공정의 $\bar{X}$ 관리도)

  • Kim, Yun-Bae;Park, Dae-Su
    • Journal of Korean Society for Quality Management
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    • v.28 no.2
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    • pp.39-56
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    • 2000
  • ${\overline{X}}$ control chart has proven to be an effective tool to improve the product quality. Shewhart charts assume that the observations are independent and normally distributed. Under the presence of positive autocorrelation and severe skewness, the control limits are not accurate because assumptions are violated- Autocorrelation in process measurements results in frequent false alarms when standard control chats are applied in process monitoring. In this paper, Threshold Bootstrap and Moving Block Bootstrap are used for constructing a confidence interval of correlated observations. Monte Carlo simulation studies are conducted to compare the performance of the bootstrap methods and that of standard method for constructing control charts under several conditions.

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Factors Influencing Nursing Students' Performance on Standard Precautions of Infection Control (간호대학생의 병원감염예방 표준주의지침에 대한 수행도에 영향을 미치는 요인)

  • Seo, Ji Hye;Jung, Eun Young
    • Journal of Korean Biological Nursing Science
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    • v.19 no.2
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    • pp.69-75
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    • 2017
  • Purpose: The purpose of this study was to identify the factors that affect nursing students' performance on standard precaution of infection control. Methods: For the analysis of the study results, mistakes and percentages, descriptive statistics, Pearson's coefficient, and multiple regression analysis were performed. Results: Among the participants, 76.5% received hospital infection education, and 72.5% experienced an exposure to blood or body fluid, and the most of the exposures were occurred during the first semester of the third year. The knowledge score was $20.08{\pm}2.09$, the awareness score was $4.63{\pm}.41$, and the performance score was $4.34{\pm}.55$. In the relationship between the standard precautions knowledge, awareness, and performance, the awareness and the performance were in positive correlations, while the factors that affected the performance level of the standard precautions were awareness and the school year (third year), which had 37.0% of the total exposures. Conclusion: In order to increase the performance level of the standard precautions among the nursing department students, after identifying the differences between school years and demands of the students, it is necessary to provide continuous and regular education of standard precautions.

Factors influencing Healthcare-associated Infection Control of Caregivers (돌봄제공자의 감염관리를 위한 표준주의 이행도 영향요인)

  • Kim, Jeeyoung;Jang, Eunhee
    • Journal of Korean Critical Care Nursing
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    • v.14 no.1
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    • pp.63-75
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    • 2021
  • Purpose : The purposes of this study were to examine the relationships between knowledge, awareness, safe environment, and performance of standard precautions and identify factors associated with performance of standard precautions. Methods : This study was a descriptive research. A structured questionnaire on knowledge, awareness, safe environment, and performance of standard precautions was used for the survey with a convenience sample of 150 caregivers. Data were collected from July to August 2019 and were analyzed using descriptive statistics, independent t-test, one way ANOVA, Pearson's correlation coefficient, and multiple regression with SPSS/WIN 25.0 program. Results : The mean scores on knowledge, awareness, safe environment, and performance of standard precautions were 15.77±3.34, 7.35±1.91, 4.55±2.05, and 55.20±10.11 respectively. Performance of standard precautions showed a statistically significant positive correlation with knowledge (r=.54, p<.001), awareness (r=.54, p<.001), and safe environment (r=.50, p<.001). Awareness (β=.24, p=.025) and safe environment (β=.35, p<.001) were significantly associated with factors of performance of standard precautions. Also, education level (high school and above college), affiliated institution (private association), and importance of infection control education (moderate) were significantly associated with factors of performance of standard precautions. Conclusion : The results of the study indicate that factors influencing the performance of standard precautions of caregivers were awareness and safe environment. Therefore, to improve implementation of the standard precautions among caregivers, a safe environment within the hospital must be supported, and appropriate infection management education needs to be provided to caregivers to improve their knowledge and awareness of the standard precautions.

Emergency Medical Technology Students' Awareness and Performance of Standard Precautions in Hospital Infection Control (응급구조과 학생의 병원감염 표준주의에 대한 인지도와 수행도)

  • Choi, Sung-Soo;Yun, Seong-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.5
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    • pp.2262-2270
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    • 2013
  • This study was to provide the basic data to raise the efficient practices that can reduce the Infection Prevention and Exposure by understanding the awareness, Performance degree of the Administrative Standard Guideline of Nosocomial Infection Control for Emergency Medical Technician students who are exposed to hospital infections while conducting clinical practice. After the study, the performance degree was lower than the awareness of the Performance degree of the Administrative Standard Guideline of Nosocomial Infection Control. And awareness and performance degree are have significant positive correlation, significant differences statistically(r=0.325, p=0.000). In the future, to improve the Performance degree of Nosocomial Infection Control for Emergency Medical Technician students, the continuing education programs and the curriculums should be performed in the practice agencies.