• Journal of Fisheries and Marine Sciences Education
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• v.29 no.2
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• pp.607-611
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• 2017

The purpose of this study is to present the concept of merchandising Styela clava HERDMAN developed in 2016. The main contents of this study are analyzing the general condition of Styela clava HERDMAN, We intend to promote stable and sustainable development of Korean aquaculture by activating the aquaculture insurance system by expanding coverage items and coverage, and increasing insurance coverage.

• Journal of Life Science
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• v.18 no.1
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• pp.109-113
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• 2008

For useful basic data in new ascidian processed products, this study investigated and compared the biological activities of two ascidians, Styela clava and Styela plicata, in two conditions with fresh one and cooking one at $100^{\circ}C$ for 10 min. By fresh state, Styela clava (8.46 mg/ml) had higher activity than Styela plicata (13.67 mg/ml) in the antioxidative assay ($EDA_{50}$), whereas Styela plicata (10.54 mg/ml) had higher than Styela clava (12.05 mg/ml) after cooking. Particularly the antioxidative activity of Styela plicata increased 1.3 times after cooking. Fresh Styela clava had antimicrobial activity against Bacillus subtilis, B. cereus, E. coli, whereas the activity appeared against E. coli only after cooking. However, antimicrobial activity in Styela plicata appeared against 4 gram positive bacteria (B. subtilis, B. cereus, S. aureus and L. monocytogenes) and E. coli, regardless of concentration level (1,000-1,500 ppm) and with/without cooking. ACE inhibitory activity was not observed in the samples. By cooking, the level of fibrinolytic activity increased to 1.79 from 1.24 plasmin unit/ml in Styela plicata, whereas the level decreased to 1.55 from 1.62 in 5tyela clava.

• Journal of Life Science
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• v.6 no.4
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• pp.250-263
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• 1996

In this study Carotenoprotein from Styela clava were extracted, and purified by ammonium sulfate fraction, Sephadex G- 200 gel chromatography and DEAE-cellulose ion exchange chromarography. The purified carotenoprotein from styela clava had absorption maxima of 487nm, 463nm and 280nm, and the carotenoid liberated from carotenoprotein had 478nm and 452nm with inflexion. One miligram of caroteno-protein contained 0.35 $\mu$g of carotenoid. The carotenoprotein had an approximate molecular weight of 398 kDa (gel filtration). SDS-PAGE showed only a single polypeptide chain with a molecular weight of 62.4 kDa. The amino acid composition of the carotenoprotein were mainly glutamic acid(11.48%), valine(10.75%), leucine (10.45%), aspartic acid(9.94%), while cysteine and tryptophan were not found. The carotenoprotein contained lipid as structure units. In the carotenoprotein, the major fatty acids were oleic acid, plamitoleic acid and palmiunsaturated fatty acids 33.6%, saturated fatty acids 18.6%. In addition, the levels of higher unsaturated fatty acids were high as much as 30.8% of the total fatty acids. Carotenoid was extracted from the carotenoprotein by acetone. Thin layer chromatography showed only carotenoid to be present. Its chemical reactivities and spectroscopic properties were studied and elucidated as astaxanthin.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.7
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• pp.908-911
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• 2006

Fish paste containing Styela clava (Korean name: miduduk) was prepared for the supplementary value of the product, and it was evaluated for the color, textural properities, and sensory attributes. Increasing the amount of S. clava in the fish paste tended to increase the lightness (L), the redness (a), and the yellowness (b) in Hunter color values. All test samples with 3 mm thickness had good flexibility and did not break even after 4 times folds. Fish paste containing 20% S. clava showed the highest values in strength, hardness, and adhesiveness. In overall acceptance of sensory evaluation, fish paste containing 5% S. clava did not show any difference in comparison with non added control. These results suggest that S. clava can be applied to fish paste products to improve the quality and functionality.

• Journal of the Korean Society of Marine Environment & Safety
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• v.19 no.6
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• pp.597-605
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• 2013

This study was conducted in warty sea squirt farm, Jindong Bay(10 stations; July, October, January, April) for the culture period of Styela clava, Styela plicata. We utilized water temperature, chlorophyll a, suspended particulate matter, hydrodynamics, salinity and dissolved oxygen saturation for the suitability assessment of warty sea squirt farm. Newly, Life Requisite Suitability graphs were made for the correlation between the growth of Styela clava, Styela plicata and farm environment. As a result of the assessment for Habitat Suitability Index, western bay(HSI 0.32~0.41) show values higher than eastern bay(HSI 0.19~0.27). This result shows the high correlation with the unit production of Styela clava, Styela plicata($R^2$=0.834, P<0.01). This could be helpful for fishery management for the sustainable productivity through the scientific suitable site assessment.

• Natural Product Sciences
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• v.13 no.2
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• pp.132-134
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• 2007

An analog of antibacterial sulfated alkene against Bacillus subtilis was isolated from a species of tunicate (Mideoduck), Styela clava, cultured at Jindong Bay, Korea. The structure was determined as 4,8-dimethyl-3-nonenyl sulfate by MS and spectral analysis such as UV IR and NMR.

• Journal of Environmental Science International
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• v.23 no.1
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• pp.89-96
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• 2014

Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and causes environmental problem. Although biological activities of S. clava were reported by many investigators, study on S. clava tunic was little. In this study, therefore, tyrosinase inhibition and antioxidative activities of extracts from S. clava tunic using different solvent were investigated for recycling of the fishery waste. Among extraction methods tested, autoclaved extraction (25.7%) and hot water extraction (18.2%) appeared to be effective for extraction. The highest total phenolic content was 46.6 mg/g in autoclaved extract while the highest flavonoid content was 23.0 mg/g in chloroform extract. All extracts possessed tyrosinase inhibition activity and the inhibition activity was concentration-dependent. Inhibition concentration ($IC_{50}$) against tyrosinase activity was $0.36{\times}10^4$ mg/ml in ethanol extract, $0.11{\times}10^3$ mg/ml in acetone extract and 0.27 mg/ml in n-butanol extract. Among extracts tested, hot water and autoclaved extracts displayed higher antioxidative activity than organic solvent extracts. Therefore, our data suggest that extract from S. clava tunic may potential candidate for cosmetic product with whitening effect and medicine for diseases caused by various oxidative stresses.

• Nutrition Research and Practice
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• v.9 no.3
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• pp.219-226
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• 2015

BACKGROUND/OBJECTIVES: In this study, potential anti-inflammatory effect of enzymatic hydrolysates from Styela clava flesh tissue was assessed via nitric oxide (NO) production in lipopolysaccahride (LPS) induced RAW 264.7 macrophages and in vivo zebrafish model. MATERIALS/METHODS: We investigated the ability of enzymatic hydrolysates from Styela clava flesh tissue to inhibit LPS-induced expression of pro-inflammatory mediators in RAW 264.7 macrophages, and the molecular mechanism through which this inhibition occurred. In addition, we evaluated anti-inflammatory effect of enzymatic hydrolysates against a LPS-exposed in in vivo zebrafish model. RESULTS: Among the enzymatic hydrolysates, Protamex-proteolytic hydrolysate exhibited the highest NO inhibitory effect and was fractionated into three ranges of molecular weight by using ultrafiltration (UF) membranes (MWCO 5 kDa and 10 kDa). The above 10 kDa fraction down-regulated LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), thereby reducing production of NO and prostaglandin $E_2$ ($PGE_2$) in LPS-activated RAW 264.7 macrophages. The above 10 kDa fraction suppressed LPS-induced production of pro-inflammatory cytokines, including interleukin $(IL)-1{\beta}$, IL-6, and tumor necrosis factor $(TNF)-{\alpha}$. In addition, the above 10 kDa fraction inhibited LPS-induced phosphorylation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinase (JNK), and p38. Furthermore, NO production in live zebrafish induced by LPS was reduced by addition of the above 10 kDa fraction from S. clava enzymatic hydrolysate. CONCLUSION: The results of this study suggested that hydrolysates derived from S. clava flesh tissue would be new anti-inflammation materials in functional resources.

• Animal Systematics, Evolution and Diversity
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• v.11 no.1
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• pp.79-86
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• 1995

A new species of ascidicolid copepod associated with a simple ascidian, Styela clava Herdman, from Chindo Island in the South Sea, belonging to the genus Botryllophilus , is described under the name of B. koreensis.

• Journal of the Korean Association of Geographic Information Studies
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• v.16 no.3
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• pp.81-90
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• 2013

The purpose of this paper is to do GIS-based suitable site selection using Scope for Growth index of Styela clava in Jindong Bay. The aquaculture of Styela calva is only conducted in Korea, especially Jindong Bay. Suspended culture of Styela clava was initiated in 2001 and the annual production reached 15,084M/T, but declined to 1,412M/T in 2005. The annual production was increased slightly to 2,484M/T in 2012 but the production is lower than the beginning yield. Scope for Growth(SFG) can indicate interrelationships between environment and organism growth index in aquaculture. GIS-based suitable site selection can be available by the concept of SFG, and fishery management system can be constructed for the sustainable production. As a result of the assessment of habitat suitability, Jindong Bay's SFG value ranges 0.054~0.57J/day and Styela clava farm's SFG values range 0.054~0.57J/day, either. The correlation between Styela clava farm's SFG and the actual production has a good result, as r=0.786, p<0.05. The construction of fishery management system using habitat suitability index ensures the reasonable site selection and the sustainable production in aquaculture farm. It introduces an objective method for the interrelationships between the environmental variation and the organism growth. Consequently, it can promote the decision making practices for the sustainable fishery management.