• Journal of Korean Society of Environmental Engineers
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• v.35 no.1
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• pp.10-16
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• 2013

Characteristics of biological hydrogen production rate and organic acid under anaerobic fermentation process were investigated with sugar wastewater. Hydrogen production rate was higher with alkaline pre-treatment than acidic pre-treatment, resulting in 70% increment. An adequate supply of the nutrients (N or P) into raw sugar wastewater could increase hydrogen production rate. Carbohydrate degradation of the anaerobic fermentation process was not directly related with hydrogen production. Sugar wastewater with the addition of the nutrients shows 3 times higher B/A ratio than the raw sugar wastewater. B/A ratio of the wastewater with alkaline pre-treatment and nutrients addition was most higher than other samples, showing 4.02 of B/A ratio. Higher B/A ratio shows higher hydrogen production rate at each sample.

• Journal of Wellbeing Management and Applied Psychology
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• v.6 no.2
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• pp.33-37
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• 2023

Purpose: Since 2013, marine dumping of wastewater has been banned, and research on eco-friendly and efficient land treatment has emerged. This study compared and tested changes in biogas production and anaerobic process efficiency depending on whether or not enzyme pretreatment was performed during anaerobic digestion from single-phase and two-phase to medium-temperature. Research design, data and methodology: The total sugar, direct sugar, pH, and acidity before and after fermentation were analyzed by G/C by anaerobic fermentation of the liquor wastewater, food wastewater 1, and food wastewater 2 at 30℃ for 67 hours, and the amount of methane gas generated was analyzed by balloon volume. Results: It was found that stable organic acid concentration and pH were found in the enzyme-treated food wastewater 2, and the amount of methane gas generated was also increased. Conclusions: When anaerobic digestion of the liquor wastewater and the food wastewater together, the performance of enzyme pretreatment resulted in increased digestive efficiency. It will be the basic data that can contribute to carbon neutrality and greenhouse gas reduction by increasing the production of biogas.

• Journal of the Korea Organic Resources Recycling Association
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• v.20 no.3
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• pp.41-51
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• 2012

Biohydrogen production and analysis of microbial community were attempted from the sugar manufacturing wastewater with anaerobic fermentation process. Addtion of nutrients ($N{\cdot}P$) into sugar manufacturing wastewater stimulates hydrogen production from 9.53 to $26.67m{\ell}$ $H_2/g$ COD. Butyric acid, acetic acid, lactic acid, and propionic acid were detected in the sample of the anaerobic fermentation process. Butyric acid/Acetic acid(B/A) ratio was increased 0.50 to 0.92 according to the nutrients addtion into the wastewater. Microbial community was analyzed as Clostridium sp. in the phylum of Firmicutes and Klebsiella sp., Erwinia sp., and enterobacter sp. of the class of $\gamma$-Proteobacteria. As the improvement of hydrogen production, Erwinia sp. was decreased and Klebsiella sp. was increased.

• Proceedings of the IEEK Conference
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• 2001.10a
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• pp.122-131
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• 2001

Pakistan being the 6$^{th}$ largest sugar producer has over 75 sugar mills with annual production capacity of about 2.4 million tons during 1996-97. The contribution of Sindh with 27 sugar mills is recorded over 50% of the total sugar production. The majority of the mills in Pakistan use the Defecation-Remelt-Phosphitation (DRP; 24 mills), Defecation-Remelt-Carbonation (DRC; 21 mills) and Defecation-Remelt Carbonation and Sulphitation (DRCS; 11 mills) process. Seven of the 75 sugar mills in Pakistan also produce industrial alcohol from molasses, a by- product of sugar manufacturing process. These sugar industries also produce fly ash, which have been found to contain unburned carbon and reach as far as four-kilo meter area with the wind direction, threatening the community health of people living around, besides posing other aesthetic problems. The untreated wastewater, in many cases, finds its way to open surface drains causing serious threat to livestock, flora and fauna. One study showed that fly ash emitted from the chimneys contain particle size ranging from 38 ${\mu}{\textrm}{m}$ to 1000 ${\mu}{\textrm}{m}$. About 50 per cent of each fly ash samples were above 300 ${\mu}{\textrm}{m}$ in size and were mostly unburned Carbon particles, which produced 85% weight loss on burning in air atmosphere at 1000${\mu}{\textrm}{m}$. This fly ash (mostly carbon) was the main cause of many health and aesthetic problems in the sugar mill vicinity. The environmental challenge for the local sugar mills is associated with liquid waste gaseous emission and solid waste. This paper discusses various waste recycling technologies and practices in sugar industries of Pakistan. The application of EM technology and Biogas technology has proved very successful in reusing the sugar industry wastewater and mud, which otherwise were going waste.

• Journal of Korean Society on Water Environment
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• v.28 no.5
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• pp.663-668
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• 2012

The feasibility of enhancing biological nutrient removal from an industrial wastewater was tested with food waste leachate and sugar liquid waste as external carbon sources. Long term influences of adding external carbon sources were investigated to see how the biological nutrient removal process worked in terms of the removal efficiency. The addition of the external carbons led to a significant improvement in the removal efficiency of nutrients: from 49% to approximately 76% for nitrogen and from 64% to around 80% for phosphorus. Approximately, 20% of the removal nitrogen was synthesized into biomass, while the remaining 80% was denitrified. Though the addition of external carbon sources improved nutrient removal, it also increased the waste sludge production substantially. The optimal observed BOD/TN ratio, based on nitrogen removal and sludge production, was around 4.0 in this study.

• The Korean Journal of Mycology
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• v.21 no.2
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• pp.127-132
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• 1993

269 yeast strains were isolated from water samples collected from different sites in Minia governorate. These included 126 strains from fresh water, 108 strains from sewage and 35 strains from wastewater from sugar-cane factory. On the basis of 23 different physiological and morphological merkmals, the isolated strains were assigned to 16 species belonging to 11 genera. Total yeast cell counts as well as spectra of yeast species were highly variable in tested water. Total yeast cell counts ranged between $3.0{\times}10^3/l\;and\;1.8{\times}10^6/l$ for fresh water, $3.0{\times}10^4/l\;and\;3.0{\times}10^7/l$ for sewage and $1.5{\times}10^6/l\;and\;2.6{\times}10^7/l$ for wastewater from sugarcane factory. Debaryomyces hansenii, Rhodotorula mucilaginosa and Torulaspora delbrueckii were the dominant species in fresh water, whereas Debaryomyces hansenii, Thrichosporon beigelii, Rhodoforula mucilaginosa and Kluyveromyces marxianus were the dominant species in sewage and Saccharomyces cerevisiae, Kluyveromyces marxianus and Trichosporon beigelii were the dominant species in wastewater from sugar-cane factory. Yeast human pathogens, Trichosporon beigelii, Rhodotorula mucilaginosa and Candida albicans were encountered in water samples indicating that water in El-Minia governorate is also polluted by some pathogenic yeasts.

• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.770-776
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• 1995

For the purpose of development of non-toxic and biodegradable flocculant, chitosan complex was isolated from Ganoderma lucidum wastes. The isolated complex was identified as the expected chitosan-glucan complex by IR specta. The complex was extracted by treatment of 50% NaOH solution at 120$\circ$C for 5 hrs, namely optimal condition and solubilized with 2% acetic acid for fur-ther use as flocculant. Preliminary experiments showed that the solubilized complex had higher flocculation activity of 1.3 fold than commercial chitosan at 400 mg/l concentration in soybean curd wastewater. Also the solubilized complex removed 83% of MLSS and 60% of COD in the soybean curd wastewater treated by photosynthetic bacteria, 50% of turbidity and 21% of MLSS in sugar industry wastewater, and 90% of turbidity and 89% of MLSS in alcohol fermentation wastewater. Bacterial cell flocculation activities of the solubilized chitosan-glucan complex were 89% in Bacillus subtilis broth, 81% in Streptococcus lactis broth, and more than 90% in Escherichia coli broth after standing for 2 days. The results reveal that chitosan-glucan complex from Ganoderma lucidum wastes can substitute for commercial chitosan as non-toxic and biodegradable flocculant.

• Journal of Life Science
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• v.6 no.1
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• pp.6-13
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• 1996

The possible use of milk-wastewater as a fermentation media for the production of citric acid by Aspergillus niger has been investigated. The addition of Mn$^{2+}$ , Fe$^{2+}$ and Cu$^{2+}$ to the medium promoted citric acid production while only Mg$^{2+}$ decreased citric acid production. The concentrations of citric acid produced were marked up to 7.2g/l and 16.5g/l in a batch bioreactor by Aspergillus niger ATCC 9142 with 50g/l and 100g/l of reducing sugar concentration in milk-wastewater, respectively. A mathematical model was developed and simulating the predictability of cell growth, citric acid production and substrate consumption rate, and gave good agreement results with experimental data.

• Korean Chemical Engineering Research
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• v.59 no.2
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• pp.174-179
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• 2021

Recently, hybrid coal research is underway to upgrade low-grade coal. The hybrid coal is made by mixing low-grade coal with bioliquids such as molasses, sugar cane, and lignin. In the case of lignin used here, a large amount of lignin is included in the wastewater of the papermaking process, and thus, research on hybrid coal production using the same is attracting attention. However, since a large amount of metal ions are contained in the lignin wastewater from the papermaking process, substances that corrode the generator are generated during combustion, and the amount of fly ash is increased. To solve this problem, it is essential to remove metal ions in the lignin wastewater. In this study, metal ions were removed by ion exchange with a alumina hollow fiber membrane coated with K-Phillipsite (K-PHI) zeolite. The alumina hollow fiber membrane used as the support was prepared by the nonsolvent induced phase separation (NIPS) method, and K-PHI seeds were prepared by hydrothermal synthesis. The prepared K-PHI seed was seeded on the surface of the support and coated by secondary growth hydrothermal synthesis. The characteristic of prepared coating membrane was analyzed by Scanning Electron Microscope (SEM), X-Ray Diffraction (XRD), Energy Dispersive Spectroscopy (EDX), and the concentration of metal ions before and after ion exchange was measured by Inductively Coupled Plasma Optical Emission Spectrometer (ICP-OES). The extraction amount of K+ is 86 mg/kg, and the extraction amount of Na+ is 54.9 mg/kg. Therefore, K-PHI zeolite membrane has the potential to remove potassium and sodium ions from the solution and can be used in acidic lignin wastewater.

• Journal of Microbiology and Biotechnology
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• v.23 no.2
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• pp.137-143
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• 2013

The diversity and distribution of methanogenic archaea in a four-compartment anaerobic baffled reactor (ABR) treating sugar refinery wastewater were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). At an organic loading rate of 5.33 kg $COD/m^3{\cdot}day$, the ABR could perform steadily with the mean chemical oxygen demand (COD) removal of 94.8% and the specific $CH_4$ yield of 0.21 l/g $COD_{removed}$. The $CH_4$ content in the biogas was increased along the compartments, whereas the percentage of $H_2$ was decreased, indicating the distribution characteristics of the methanogens occurred longitudinally down the ABR. A high phylogenetic and ecological diversity of methanogens was found in the ABR, and all the detected methanogens were classified into six groups, including Methanomicrobiales, Methanosarcinales, Methanobacteriales, Crenarchaeota, Arc I, and Unidentified. Among the methanogenic population, the acid-tolerant hydrogenotrophic methanogens including Methanoregula and Methanosphaerula dominated the first two compartments. In the last two compartments, the dominant methanogenic population was Methanosaeta, which was the major acetate oxidizer under methanogenic conditions and could promote the formation of granular sludge. The distribution of the hydrogenotrophic (acid-tolerant) and acetotrophic methanogens in sequence along the compartments allowed the ABR to perform more efficiently and steadily.