• 한국발생생물학회지:발생과생식
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• 제10권1호
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• pp.19-32
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• 2006

참게(Eriocheir sinensis)와 꽃게(Portunus trituberculatus)의 2종으로부터 genomic DNA를 분리 추출하였다. 선택된 7개의 OPA-05, OPA-13, OPA-16, OPB-06, OPB-15, OPB-17 and OPD-10의 RAPD primer를 이용하여 identical, polymorphic 그리고 specific fragment를 얻어냈다. 본 연구에서 부안산 참게 집단에서는 505개의 fragment가 나타났고, 꽃게 집단에서는 513개의 fragment가 확인되었다. 참게 집단에서는 165개의 identical fragment가 나타났으며, 이는 primer당 평균적으로 23.6개의 fragment로 확인되었다. 또한 꽃게 집단에서는 66개로서 평균해서 primer당 9.4개의 identical fragment가 나타났다. 참게 집단과 꽃게 집단의 polymorphic fragment는 각각 50개와 14개로 나타났고, 참게 집단과 꽃게 집단의 경우 OPB-17에서 identical fragment가 300 bp의 크기에서 확인되었다. 각각을 비교해 보았을 때 유전적 차이는 참게 집단에서보다 꽃게 집단에서 더 높은 수치를 나타내었고, 2종 사이에서 $0.726{\pm}0.004$의 수치를 나타내었다. 7개의 primer를 사용하여 얻어진 dendrogram은 cluster 1(FRESHWATER 01), cluster 2(FRESHWATER 02, 03, 04, 05 및 06), cluster 3(FRESHWATER 07, 08, 09, 10 및 11) 및 cluster 4(SWIMMING 12, 13, 14, 15, 16, 17, 18, 19, 20, 21 and 22)와 같이 4개의 유전적 클러스터로 나뉘어졌다. 꽃게 집단에서 18번째 개체(SWIMMING no. 18)와 17번째 개체 (SWIMMING no. 17) 사이가 가장 가까운 유전적 관계(genetic distance 0.096)를 나타내었다. 궁극적으로 볼 때 참게 집단의 2번째(FRESHWATER no. 02)와 참게 집단의 3번째(FRESHWATER no. 03) 개체 사이가 가장 먼 유전적 거리 (genetic distance=0.770)를 나타내었다. 위에서 언급했던 것처럼 RAPD-PCR 방법은 참게 및 꽃게 2종의 종 판별을 하기 위한 진단적 표지 (diagnostic marker)로 이용할 수 있는 잠재력을 가지고 있는 것으로 확인되었다.

• 수산해양기술연구
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• 제50권3호
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• pp.262-273
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• 2014

To investigate the catching efficiency of a biodegradable round trap for a swimming crab (Portunus trituberculatus), four types of material (two types of biodegradable net, one type of biodegradable plus polyethylene net and one type of polyethylene net) for traps were used in the field test, and the field tests were carried out 11 times in the Boryeong fishing ground of Korea, 2012~2013. The catching efficiency of four type traps was analyzed by catch per trap, weight per trap and mean weight per individual of small and large size swimming crab. Statistical Kruskal-Wallis test was carried out to verify the significant difference for the efficiency between types of material. The catch in number of swimming crab was 9,015 and formed about 94.3% of total catch. In the results of catching efficiency of traps, there was a little difference in catch per trap, weight per trap and mean weight per individual of swimming crab in-situ data. But there was no significant difference in catching efficiency between the biodegradable traps and the PE trap (P>0.05), so it's needed to use widely a biodegradable trap for the conservation and sustainable management of swimming crab resources.

• 수산해양기술연구
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• 제48권4호
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• pp.322-336
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• 2012

To study the catching efficiency of biodegradable trammel net for swimming crab (Portunus trituberculatus), three types (biodegradable, monofilament and multifilament) of trammel nets were used in the field test, and the tests were carried out 16 times with two different mesh sizes (105mm and 160mm) in the Yeonpyeong fishing ground of Korea, 2009~2011. The catching efficiency of three type nets was analyzed by catch in number, catch in weight and average weight per individual of small and large size swimming crab by net types and mesh sizes. Statistical T-test was also carried out to verify the efficiency between the three types of nets. The results are as follows. The catch in number of swimming crab was 24,667 and formed about 81.0% of total catch. Of all swimming crab catch, small swimming crabs with less than 64mm in carapace length which is a prohibited landing size by law formed 48.1%, larger swimming crabs with more than 64mm in carapace length which is a landing size formed 51.9%. In 105mm mesh size trammel net test, the catch share in number of small size swimming crab by biodegradable trammel net was 47.5% in comparison with multifilament trammel net and 74.2% in comparison with monofilament trammel net, so biodegradable trammel net has more protective effects on small size swimming crab than other types of trammel nets. The protective effects for small size swimming crab by biodegradable trammel net was 25.8~52.5% in comparison with other types of trammel nets. The catch share in weight of large size swimming crab by biodegradable trammel net was 98.3% in comparison with multifilament trammel net and 92.3% in comparison with monofilament trammel net, so biodegradable trammel net has a similar catch efficiency to multifilament trammel net. The results of 160mm mesh size trammel net test have shown similar results of 105mm mesh size trammel net test. This study shows that biodegradable trammel net is a more useful fishing gear than multifilament and monofilament trammel net because biodegradable trammel net has lower catch rate than other types of nets in small size swimming crab and similar catch rate than multifilament trammel net which is a well used net by fishermen.

• 수산해양기술연구
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• 제49권2호
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• pp.106-115
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• 2013

A series of fishing experiments were carried out in the western coastal waters of Korea from May, 2006 to September, 2007, using gill nets of different mesh sizes (m=101, 121.8, 152.5, 176.8, and 191 mm) to determine the mesh selectivity of gill net for swimming crab, Potunus trituberculatus. The catch species was composed of swimming crab (87%), Thomas's rapa whelk Rapana venosa (5%), arthritic neptune Neptunea cumingi (2%), Japanese swimming crab Charybdis japonica (2%), marbled sole Pleuronectes yokohamae (1%), pen shell Atrina pectinata, ocellate spot skate Okamejei kenojei, and so on. The carapace length (CL) of each swimming crab caught in the experimental nets was measured. Sponge crab (egg-bearing female crab) was appeared from the end of June to August in the coastal waters. The master selection curve was estimated by applying the extended Kitahara's method. The selection curve showed that the gill nets of larger mesh size allowed more crabs of small carapace size to escape. The optimum value (CL/m) was 0.645 and the CL/m was estimated to be 0.301, 0.354, 0.391, 0.422, and 0.450 when the retention probabilities were 0.1, 0.2, 0.3, 0.4 and 0.5, respectively.

• 수산해양기술연구
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• 제45권4호
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• pp.251-259
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• 2009

Swimming crabs, Portunus trituberculatus(Miers) are commercially important off the coasts of Korea, Japan and China. Harvest of swimming crabs has been fluctuated along their distribution ranges. Fluctuations in the interannual harvest of swimming crabs may be correlated with the survival rate during the larval period. The survival rates, intermolt periods, and growth of larval swimming crabs were investigated in the laboratory. Larval swimming crabs are released and undergo development from April to August off the western coast of Korea in the Yellow Sea. Sea surface temperatures off the western coast of Korea during the larval season were used for the laboratory experiments, and ranged from 22 to 26${^{\circ}C}$. Larvae were individually cultured at four different temperatures, 22${^{\circ}C}$, 24${^{\circ}C}$, 26${^{\circ}C}$, and 28${^{\circ}C}$. Zoea molted to megalopa at all temperatures and developed to the first crab stage at 24${^{\circ}C}$, 26${^{\circ}C}$, and 28${^{\circ}C}$. Survival rates from zoea I to the first crab stage increased with increasing temperatures. Intermolt period and the growth rate of the mean carapace length were inversely correlated with temperature. Our research helps understand the changes in survival rate and growth of larval swimming crabs resulting from changing oceanic temperatures. Further, our study suggests that the fluctuations in fishery harvest of swimming crabs off the coast of Korea may be related to changes in larval survival affected by changing ocean conditions.

• 한국수산과학회지
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• 제50권3호
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• pp.243-249
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• 2017

Crabs are a popular seafood item. However, they can harbor many microorganisms, heavy metals, radioactivity, and benzo(a)pyrene, which are potential health risks to humans. The objective of this study was to assess the potential of swimming crabs for use in foods such as Ganjang-gejang, by measuring their sanitary biological and chemical properties. Viable microbial cell counts in swimming crab samples were $3.4{\times}10^2-6.7{\times}10^4CFU/g$, but no coliform, Escherichia coli, or pathogenic bacteria, such as Vibrio parahaemolyticus, Enterohemorrhagic E. coli, Listeria monocytogenes, Staphylococcus aureus, or Salmonella spp., were detected. Heavy metal concentrations in swimming crab samples were non-detectable to 0.112 mg/kg for total mercury, non-detectable to 0.435 mg/kg for lead, and 0.115-0.836 mg/kg for cadmium. Benzo(a)pyrene concentrations ranged from $0.025-0.060{\mu}g/kg$, and the volatile basic nitrogen content ranged from 8.7-15.6 mg/100 g. No radioactivity was detected in samples. These results suggest that swimming crabs are viable for use in seafood products.

• Ocean and Polar Research
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• 제39권3호
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• pp.213-219
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• 2017

In this study, we analyzed the economic feasibility of the swimming crab (Portunus trituberculatus) fry releasing program in the West Sea of Korea. The catch rate of released fry in 2010, measured by genetic markers, was applied to the economic surplus method to estimate benefits. As a result of our analysis, the B/C ratio, as an indicator of economic evaluation, was determined to be 2.168, which means that the releasing program was economically feasible. And it was shown that the benefits to consumers is six times greater than the benefits to producers, confirming the necessity of the releasing program as a public work.

• Journal of the Korean Data Analysis Society
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• 제20권6호
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• pp.2941-2951
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• 2018

본 연구는 해양기상관측자료인 평균 풍속, 평균 기압, 평균 상대습도, 평균 기온, 평균 수온, 평균 최대파고, 평균 유의파고, 최고 유의파고, 최고 최대파고, 평균 파주기, 최고 파주기 등의 요인들이 꽃게의 출하건수에 미치는 영향을 음이항 회귀모형을 통해 확인하고 모형적합을 시도하였다. 염분과 수온이 갑각류의 성숙 및 산란에 영향을 미치며, 특히 수온은 성장에 관여하는 대사 작용에 영향을 끼친다고 알려져 있고 최근 지구온난화로 인해, 얼음이 녹으면서 바다의 유의, 최대, 평균파고와 파주기, 그리고 수온까지 영향을 미치고 있어 꽃게 출하건수를 예측하는데 있어 중요한 변수라고 생각할 수 있다. 분석결과 꽃게의 출하건수에 영향을 주는 요인은 평균 풍속, 평균 기압, 평균 상대습도, 평균 해수온도, 최대 파고, 평균 파주기, 최대 파주기로 결정되었다. 꽃게의 출하건수는 평균 풍속, 평균 기압, 평균 상대습도, 평균 해수온도, 평균 파주기가 높을수록 증가하는 경향을 보이고 있고, 최대 파고, 최대 파주기가 낮을수록 꽃게의 출하건수는 증가하는 경향을 보이고 있었다.

• Animal cells and systems
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• 제13권3호
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• pp.305-314
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• 2009

The control region of mitochondrial DNA (13516-14619) is located between srRNA and $tRNA^{lle}$ gene in swimming crab, Portunus trituberculatus. The present study was investigated the genetic polymorph isms of the control region in samples of P. trituberculatus collected at coastal waters of the Yellow Sea in Korea. A total of 300 substitution and indel polymorphic sites were identified. In addition to SNPs and indel variation, a hypervariable microsatellite motif was also identified at position from 14358 to 14391, which exhibited 10 alleles including 53 different suballeles. When the hypervariable microsatellite motif was removed from the alignment, 95 haplotypes were identified (93 unique haplotypes). The nucleotide and haplotype diversities were ranged from 0.024 to 0.028 and from 0.952 to 1.000, respectively. The statistically significant evidence for geographical structure was not detected from the analyses of neighbor-joining tree and minimum-spanning network, neither. This result suggest that population of P. trituberculatus are capable of extensive gene flow among populations. We believed that the polymorph isms of the control region will be used for informative markers to study phylogenetic relationships of P. trituberculatus.

• 수산해양교육연구
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• 제25권4호
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• pp.991-997
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• 2013

Distribution and occurrence of swimming crab, Portunus trituberculatus larvae were investigated in the mid-western coast of Korea in the Yellow Sea. P. trituberculatus larvae were collected in July and August from 2010 to 2012. Bongo net with 303 mesh was deployed once with a double oblique tow. Zoea I (ZI) densities were highest in all sampling months. Then densities of later larval stages decreased dramatically. In general, larval densities at the stations in northern parts and coastal areas were higher than those at the southern and offshore area. Because egg bearing seasons of P. trituberculatus in the study area are between April and August, larval densities, particularly, of ZI may be underestimated. Considering higher densities of ZI and lower ones of later stages, larvae may be transported to growing area and returned to the parental populations. Larval densities and sea surface temperature were not correlated.