• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2008년도 추계학술발표회
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• pp.487-488
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• 2008

• 한국광과학회:학술대회논문집
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• 한국광과학회 2001년도 학술대회지
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• pp.35-35
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• 2001

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2002년도 추계학술대회
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• pp.221.2-221.2
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• 2002

• 한국광과학회:학술대회논문집
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• 한국광과학회 2005년도 제12회 학술대회 논문초록
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• pp.113-113
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• 2005

• Journal of Plant Biotechnology
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• 제35권2호
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• pp.141-145
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• 2008

본 연구는 남세균 고유의 프로모터를 포함하는 유전자간 염기서열에 기반하여 환경위해성 검출용 바이오센서를 개발하고자 시도되었다. 포도당 처리에 의해서 유도되는 8종의 유전자 (atpI, ndbA, ctaD1, tkt, pgi, pdh, ppc, 그리고 rydA)의 프로모터 부위를 리포터 유전자의 일종인 발광유전자 (luxAB) 벡터 pILA (Genbank: AJ251840)에 도입시켜 재조합 벡터를 제조한 후 Synechocystis sp. PCC6803을 형질전환시킨 결과, pILA 벡터만을 포함하고 있는 대조구에 비해서 포도당 처리에 의해서 생물발광량이 5-25배 정도 현저히 증가함을 확인하였다. 또한 $Hg^{2+}$, $Cu^{2+}$, $Zn^{2+}$과 같은 중금속, $CN^-$, DCMU, DBMIB와 같은 제초제, 그리고 클로람페니콜이나 리팜피신과 같은 항생제에 의해서 생물발광이 현저히 억제되었다.

• Journal of Photoscience
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• 제3권1호
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• pp.23-28
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• 1996

To investigate the structure and function of photosystem I, cartridge mutagenesis technique was used to inactivate the psaB gene of photosystem I. From the screen, many strains which have potential defects in photosystem I were generated. Biochemical analysis revealed that B2, one of the mutant, had a reduced amount of chlorophyll. Electron transfer activitx from photosystem II to photosystem I as oxygen uptake was the rate of 64 % of wild type. Also B2 showed a decreased photosystem I activity when measured by 77 K fluorescence emission spectrum. Particularly, immunodetection analysis showed that the B2 had reduced amount of PsaA/PsaB, but a normal range of PsaC and PsaD. Here we present a photoheterotrophic mutant for psaB gene as a unique model strain for future study of structural/functional relationship and biogenesis of photosystem I.

• 한국광과학회:학술대회논문집
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• 한국광과학회 2000년도 학술대회지
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• pp.17-17
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• 2000

• 한국광과학회:학술대회논문집
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• 한국광과학회 2000년도 학술대회지
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• pp.18-18
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• 2000

• 한국식물학회:학술대회논문집
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• 한국식물학회 2000년도 춘계학술발표대회:발표눈문요지록
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• pp.24-24
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• 2000

• Journal of Microbiology and Biotechnology
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• 제12권1호
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• pp.114-120
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• 2002

A 1,989-bp genomic region encoding nickel resistance genes was isolated from Legionella pneumophila, a pathogen for legionellosis. From a sequencing and computer analysis, the region was found to harbor two structural genes, a nreB-like protein gene (1,149 bp) and a nreA-like protein gene (270 bp), in a row. Both genes exhibited a significant degree of similarity to the corresponding genes from Synechocystis sp. PCC6803 ($54\%$ amino acid sequence identity) and Achromobacter xylosoxidans 31A ($76\%$). The gene was successfully expressed in E. coli MG1655 and conferred a nickel resistance of up to 5 mM in an LB medium and 3 mM in a TMS medium including gluconate as the sole carbon source. E. coli harboring the nickel resistance gene also exhibited a substantial resistance to cobalt, yet no resistance to cadmium or zinc. Since the extracellular concentration of nickel remained constant during the whole period of cultivation, it was confirmed that the nickel resistance was provided by an efflux system like the $Ni^2＋$permease (nrsD) of Synechocystis sp. strain PCC6803. Since polyphosphate (poly-P) is known as a global regulator for gene expression as well as a potential virulence factor in E. coli, the nickel resistance of a ppk mutant of E. coli MG 1655 harboring the nickel resistance gene from L. pneumophila was compared with that of its parental strain. The nickel resistance was significantly attenuated by ppk inactivation, which was more pronounced in an LB medium than in a TMS medium.