• 제목/요약/키워드: TBARS absorbances

검색결과 3건 처리시간 0.023초

고 pH 돈육 내의 항산화 기작 (Factors Affecting Lipid Oxidation Development in High pH Ground Pork)

  • 황기;김혁일;하영득
    • 한국식품과학회지
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    • 제25권5호
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    • pp.517-520
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    • 1993
  • 돈육의 pH가 5.0에서 7.0으로 증가할수록 저장중 산패치는 유의차 있게 감소하였다. 본 실험 결과 고 pH 돈육의 낮은 유리철 함량과 높은 GPx 활성은 저장중 돈육의 산패 억제에 관여하는 중요한 요인들인 것으로 밝혀졌다. 그러나 pH 조절에 의한 산패 억제에 관여할 수 있는 그 밖의 다른 가능한 인자들을 찾는 연구 또한 중요하다고 하겠다. 나아가서 유리철 ion의 방출 억제나 불활성화 방안, GPx의 또 다른 활성화 방안 및 천연항산화제로서의 활용 가능성등이 계속 연구, 검토되어야 할 것이다. 본 연구의 결과는 돈육의 저장성 증진은 물론, 규명된 항산화 기작들을 다른 작품 system에 응용하는 데에도 이용될 수 있을 것으로 본다.

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취반 재고미를 첨가하여 제조한 저지방 분쇄우육의 냉중중 안정성 (Microbiological and Oxidative Stability of Low Fat Ground Beef during Refrigeration)

  • 김혁일
    • 한국축산식품학회지
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    • 제18권4호
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    • pp.301-306
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    • 1998
  • Four low fat ground beef groups containing 10% fat plus 0, 5, 10 and 20% additional cooked lod rice and a control ground beef containing 30% fat were prepared and the analysis for microbiological and oxidative stability were conducted. During 6 days of storage at 4$^{\circ}C$ microbial analysis including total plate count and coliform groups were performed and 2-thiobarbituric acid reactive substance (TBARS) absorbances were measured. The growth rate of total aerobic bacteria and coliform groups tended to increase with the increase in fat content and the amount of added cooked lod rice. Development of oxidative rancidity were not significantly different between 10 and 30% fat ground beef but among the 10% ground beef the rancidity development significantly(p<05) decreased with the in-crease in the amount of added cooked old rice. Low fat ground beef groups were not stable over 3 days during storage at 4$^{\circ}C$.

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High Performance Liquid Chromatography (HPLC) Detection of Malonaldehydethiobarbituric Acid (MA-TBA) Complex in Ground Pork

  • Whang, Key
    • Preventive Nutrition and Food Science
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    • 제4권3호
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    • pp.171-174
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    • 1999
  • For monitoring lipid oxidation development in cooked ground pork during refrigerationm, malonaldehydethiobarbituric acid(MA-TBA) contents were measured using high performance liquid chromatography(HPLC). As the oxidation proceeded during refergeration, TBA-reaction substances(TBARS) absorbances increased and the corresponding HPLC peak areas also increased proportationately. The correlation coefficient between the HPLC peak areas and MA-TBA absorbance were 0.9979. The treatemtn of cetrimide, an ion pairing agent, gave a complete resolution of the MA-TBA complex and the butanol extraction of the complex increased its recovery by 37.8%. Both cetrimide treatment and butanol extraction are essential steps for analyzing MA-TBA complex in ground pork wiht HPLC. A reliable and specific measurement of NA-TBA in ground pork was successfully performed using HPLC.

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