• YAKHAK HOEJI
• /
• v.54 no.4
• /
• pp.226-231
• /
• 2010

Oral fluid has become increasingly popular as an alternative specimen in the field of driving under the influence of drugs (DUID) and work place drug testing. In this study, an analytical method for the detection and quantification of ${\Delta}^9$-tetrahydrocannabinol (THC) and its metabolite, 11-nor-9-carboxy-${\Delta}^9$-tetrahydrocannabinol (THC-COOH) in oral fluid by SPE and GC-MS was established and fully validated. The stability of THC and THC-COOH in oral fluid during storage was also determined by examining the THC and THC-COOH concentration changes depending on time and container materials. Oral fluid samples were kept over 21 days at room temperature, $-4^{\circ}C$ and $-20^{\circ}C$ in two different specimen collection tubes; glass and polypropylene tubes. Three replicates for each condition with different temperature and types of a container were analyzed at five different time points over 21 days. When oral fluid samples were stored in glass tubes, the loss of both THC and THC-COOH was less than 10% at all room temperature, $-4^{\circ}C$ and $-20^{\circ}C$. However, in polypropylene tubes, the loss of both THC and THC-COOH increased significantly over the study period. In particular, the concentration of THC decreased more rapidly than that of THC-COOH at room temperature and the maximal percentage of THC lost was 90.3% after 21 days. The result indicates that it would be necessary to collect oral fluid samples in glass containers and cool the samples until analysis in order to prevent the degradation of analytes.

• YAKHAK HOEJI
• /
• v.48 no.3
• /
• pp.207-212
• /
• 2004

An analytic method was developed for the quantitation of $\Delta$$^{9}-$ tetrahydrocannabinol (THC) and 11-nor-9-carboxy THC (THC-COOH) in human hair. After hair samples were pulverized using Freezer Mill, deuterated internal standards were added and digested in 1 N NaOH at $100^{\circ}C$ water bath for 30 min. Digest solutions were extracted by 5 ml hexane：ethyl acetate (90：10) after acidification with acetic acid. The organic phase was evaporated under N 2 and derivatized by BSTFA (with 1% TMCS) at $85^{\circ}C$ for 45 min. The derivatized solution was separated on HP-5MS column ($30m{\times}0.25mm{\times}0.25mm$) and detected using EI-GC-MS with selective ion monitoring mode. The assay of calibration was ranged from 5 to 100 ng/50 mg hair ($r^2$＞0.99) for THC and THC-COOH. Within and between-run precision were calculated at 6, 30, 60 ng/50 mg hair with coefficients of variation less than 11%. Within and between run accuracies at the same concentrations were$\pm$14% and $\pm$30% of target for both analytes, respectively. Absolute and relative recovery at 10 and 100 ng were 60∼91％. The method was used to detect and quantify THC and THC-COOH in cannabis abuser's hairs (N = 16) and SRM (N=5, THC 1 ng/mg, NIST). We detected THC and THC-COOH in only one hair sample. In SRM, % accuracy was 93% (range 86∼103%) and precision (% CV) was 8.14. We began to set up a quantitative analysis of THC and THC-COOH using EI-GC-MS. Continuously, we need to modify and develop this method in order to apply for identification in cannanbis users' hair.

• Biomolecules & Therapeutics
• /
• v.21 no.1
• /
• pp.60-65
• /
• 2013

3,4,5-Trihydroxycinnamic acid (THC) is a derivative of hydroxycinnamic acids, which have been reported to possess a variety of biological properties such as anti-inflammatory, anti-tumor, and neuroprotective activities. However, biological activity of THC has not been extensively examined. Recently, we reported that THC possesses anti-inflammatory activity in LPS-stimulated BV2 microglial cells. However, its precise mechanism by which THC exerts anti-inflammatory action has not been clearly identified. Therefore, the present study was carried out to understand the anti-inflammatory mechanism of THC in BV2 microglial cells. THC effectively suppressed the LPS-induced induction of pro-inflammatory mediators such as NO, TNF-${\alpha}$, and IL-$1{\beta}$. THC also suppressed expression of MCP-1, which plays a key role in the migration of activated microglia. To understand the underlying mechanism by which THC exerts these anti-inflammatory properties, involvement of Nrf2, which is a cytoprotective transcription factor, was examined. THC resulted in increased phosphorylation of Nrf2 with consequent expression of HO-1 in a concentration-dependent manner. THC-induced phosphorylation of Nrf2 was blocked with SB203580, a p38 MAPK inhibitor, indicating that p38 MAPK is the responsible kinase for the phosphorylation of Nrf2. Taken together, the present study for the first time demonstrates that THC exerts anti-inflammatory properties through the activation of Nrf2 in BV2 microglial cells, suggesting that THC might be a valuable therapeutic adjuvant for the treatment of inflammation-related disorders in the CNS.

• Proceedings of the PSK Conference
• /
• 2002.10a
• /
• pp.194-199
• /
• 2002

Many oxidative metabolites of tetrahydrocannabinols (THCs), active components of marijuana, were pharmacologically active, and 11-hydroxy-THCs, 11-oxo-${\Delta}^8$-THC, 7-oxo-${\Delta}^8$-THC, 8$\beta$, 9$\beta$-epoxyhexahydrocannabinol (EHHC), 9$\alpha$, l0$\alpha$-EHHC and 3'-hydroxy-${\Delta}^9$-THC were more active than THC in pharmacological effects such as catalepsy, hypothermia and barbiturate synergism in mice. Cannabidiol (CBD), another major component, was biotransfomred to two novel metabolites, 6-hydroxymethyl-${\Delta}^9$-THC and 3-pentyl-6, 7, 7a, 8, 9, lla-hexahydro-I, 7-dihydroxy-7, 1O-dimethyldibenzo[b, d]oxepin (PHDO) through 8R, 9-epoxy-CBD and 85, 9-epoxy-CBD, respectively. Both metabolites exhibited some pharmacological effects comparable to d9 - THe. Cannabinol (CBN), the other major component, was mainly metabolized to ll-hydroxy-CBN by hepatic microsomes of animals including humans. The pharmacological effects of the metabolite were higher than those of CBN demonstrating that II-hydroxylation of CBN is metabolic activation pathway of the cannabinoid as is the case in THCs. Tolerance and reciprocal cross-tolerance developed to pharmacological effects d8 - THC and ll-hydroxy-d8-THC , and the magnitude of tolerance development produced by the metabolite was significantly higher than that by d8-THC. The results indicate that ll-hydroxy-d8-THC has an important role not only in the pharmacological effects but also its tolerance development of d8 - THe. THCs and their metabolites competed to the specific binding of CP-55, 940, an agonist of cannabinoid receptor, to synaptic membrane from bovine cerebral cortex. The Ki value of THCs and their metabolites were closely paralleled to their pharmacological effects in mice. A novel cytochrome P450 (cyp2c29) was purified and identified as a major enzyme responsible for the metabolic activation of d8-THC at the II-position in the mouse liver. cDNA of CYP2C29 was cloned from a mouse cDNA library and its sequence was determined. The oxidation mechanism of THC by cyp2c29 was proposed.

• Animal cells and systems
• /
• v.3 no.2
• /
• pp.215-219
• /
• 1999

Monoclonal antibody for delta-9-tetrahydrocannabiol (THC Ab), conjugated with protein A-gold, was employed as a probe to detect THC localization in the gland and subjacent cells of chemically fixed bracts of Cannabis. THC was detected in the outer wall of the disc cells, fibrillar matrix, the surface feature of secretory vesicles, and sheath throughout development of the secretory cavity. The probe was absent from vesicles. Label was also present in anticlinal walls of disc cells and walls of dermal and mesophyll cells. Little or no THC Ab was present in disc cells and none were detected in control tissues. This distribution pattern of THC Ab was similar to that in tissues prepared by high pressure cryofixation-cryosubstitution. Consistent association of THC with wall and wall-derived materials suggests that cannnabinoids are synthesized outside the plasma membrane and bound to a wall component, where-upon they are transported to the cavity with wall materials released from the disc cell wall during development of the secretory cavity.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2002.04b
• /
• pp.101-104
• /
• 2002

The beam splitter is important in optical communication systems. in this work, thc quasi-state based on four-mode interference in thc MMI coupler is proposed, and so, device length is shorten to be 1/5 of general designed length. We designed thc polarization splitter based on thc concept of quasi-state. Thc analysis has been accomplished by thc effective index method(EIM) and thc mode propagation analysis(MPA) for given structure.

• Journal of Korean Library and Information Science Society
• /
• v.2
• /
• pp.1-21
• /
• 1975

Thc purposc of this .study is for ri~aking a model of building a library collcction dcnizn~cd on th.3 curriculum through thc inter-comparison of 15 standards for collcgc ant1 anivcrsily libraries in 5 countries (U. S. A.. Great Ikitain, Canada, Japan, and Icorca) Thc rcsult of thcscl colnpsrisons is as follow.. (1) Qu;~nlitalivc scope: of thc cull(:ction: (r/ Standards for a minimal collcction arc presented to about 100,000 vols (20-30 vols per student) for a ncw university and about 40,000 vols (40 vols pcr studcnt) for a nrnr collcge. $)$ Standards for a working collcction arc prescnted to 70-80 vols per student for a univer:aity and 50-60 vols per student for a college. $$ Standard for an intcnsive rescc~rch collcction is presented to morc than 100 vols p::r studcnt. (2) Stop: of the annual incrc;lsc cf thc collection: (i: Standard for a minimal collcction is ~~rescntcdto 1.4-2 vols per studcnt. $$ Standard for an intensive rrscarch or working collection is pcrsented to 4-5 vols pcr studcnt. (3) Scope of thc anni.1a1 budgetary standards: In Grcat I3ritain and U.S. A.. th-re aro assigned to 1 . 5 ~ 2 % of the collcge and university cxpcnditurr for books. $,$ In our country, it is prcscntcd to 3.5% of thc collcge and university expenditure for books, whcn my calculating by thc various factor analysis.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.3317-3322
• /
• 2007

THC(Total Hydrocarbon) emissions during cold start and warm-up phase constitute the majority of THC emissions during the FTP-75 mode. As the basic approach to improve the emission performance of Gasoline engine during transient phase, the effect of spark timing retard from MBT on THC emission characteristics is studied by engine test using a Fast response Flame Ionization Detector(FFID). A cyclic analysis of the combustion process shows that high THC emissions are produced first few cycles during the transient phase. This paper presents the results of engine performance and emission of Gasoline engine with various spark timing. consequently, This paper was focused on the combustion phenomena with various spark timing during transient phase which was analyzed by Fast response Flame Ionization Detector (FFID) equipment to measure the cyclic THC emission characteristics.

• YAKHAK HOEJI
• /
• v.17 no.1
• /
• pp.21-26
• /
• 1973

The constituents of Korean cannabis were studied comparatively with foreign orgins. Especially, tetrahydrocannabinol(THC), the active constituents of cannabis were analyzed by the technique of thin layer chromatography and colorimetry. The results are as follows ; (1) THC content in Korean cannabis is comparatively higher than that in foreign samples. (2) THC is contained most abundantly in male flowers, abundantly in female tops and leaves and some in barks. (3) There is a tendency that the THC content increase gradually with growth of plants, being highest during unripe nad decrease with maturity of the tops. (4) Korean cannabis contains THC, cannabinol, cannabidiol, cannabidiolic acid and other cannandiolic compounds. Distribution of chemical components of Korean cannabis, compared with those of foreign ones, is remarkably different. (5) The THC content of Spanish cannabis cultivated tentatively in Korea is similar to that of Korean cannabis.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.5 no.5
• /
• pp.213-218
• /
• 1997

CNG dual fuel engine for heavy duty diesel engine has been equipped to a Korean bus engine and tested to compare th engine performance and the emission characteristics with the existing diesel fueled engine. The results are summarized as follows. Diesel fueled engine has the fuel injection timing of BTDC17°. The injection timing of CNG modified engine is retarded to BTDC14° for reduction of NOx. Performance optimization has been carried out to have engine power equivalent to or better than the diesel fueled engine. Smoke is decreased by 85% by Korean smoke 3 mode test. By 6 mode test CO is increased by 313% and THC is increased by 1407%. NOx is decreased by 27%. Even though THC is increased very much, it's not too serious problem since CO and THC emission of diesel engine are very little compared to gasoline engine and THC don't give bad effect on human health. But the reduction technologies of CO and THC need to be considered.