• Title, Summary, Keyword: Theileria sergenti

Search Result 42, Processing Time 0.043 seconds

Analysis of partial cDNA sequence from Theileria sergenti

  • Park, Jin-ho;Chae, Joon-seok;Kim, Dae-hyuk;Jang, Yong-suk;Kwon, Oh-deog;Lee, Joo-mook
    • Korean Journal of Veterinary Research
    • /
    • v.39 no.4
    • /
    • pp.797-805
    • /
    • 1999
  • T sergenti cDNA library were constructed to get a more broad information about the structural, functional or antigenic properties of the proteins, and analyzes for their partial cDNA sequences and expression sequences tags(ESTg). The mRNA were purified from T sergenti isolates to identify the information of antigen gene, then first and second strand cDNA was synthesized. EcoR I adaptor ligation and Xho I enzyme restriction were used to the synthesized cDNA, and ligated into a Uni-ZAP XR vector. T sergenti cDNA library was constructed with packaging and amplification in vitro. Antibody screening was performed with constructed T sergenti cDNA library using antisera against T sergenti. Among those clones, eight phagemids were rescued from the recombinant in vivo excision with f1 helper phage. Using the analysis of endonuclease restriction and PCR, the recombinant cDNA were proved having a 0.5-3.0kb of inserts. The eight of partial cDNA clones' sequences were obtained and examined for their homology using BLASTN and BLASTX. The eight of sequenced clones were classified into three groups according to the basis of database searches. A total 3,045bp of partial cDNA sequence were determined from six clones. The putatively identified clones contain a cytochrome c gene, a heat shock protein gene, a cyclophilin gene, and a ribosomal protein gene. The unidentified clones have a homology to ATP-binding protein(mtrA) gene of S argillaceus, DNA-binding protein(DBP) gene of Pseudorabies virus 85kDa merozoite protein gene of B bovis, mRNA spm1 protein of T annulata and glycine-rich RNA-binding protein mRNA of O sativa etc.

  • PDF

Influence of Dexamethasone on the Recrudescence of Theileria sergenti in Carrier Calves (Theileria sergenti 불현성감염우(不顯性感染牛)에 대(對)한 Dexamethasone의 영향(影響)에 관(關)한 연구(硏究))

  • Son, Jae-young;Ko, Ki-whan
    • Korean Journal of Veterinary Research
    • /
    • v.27 no.1
    • /
    • pp.93-99
    • /
    • 1987
  • A study was conducted to observe the effects of dexamethasone sodium 21 phosphate injection in different amounts, intervals and times on the fluctuation of the parasitized erythrocytes in the peripheral blood of the T. sergenti carrier calves. A total of 31 calves including 15 Holstein and 16 Korean native male calves aged 6-12 months (125-300kg) was used. Results observed were as follows. 1. The calves injected 0.2mg/kg body weight two and three times in 48 hour intervals and 5 times in 24 hour intervals observed that 2 out of 2 heads, 3 out of 6 heads, and 4 out of 5 heads, respectively were substantially increased parasitized erythrocytes in the blood for 3-11 days after the first injection and continued to 2-10 days. 2. However, the calves injected once with 0.1mg/kg body weight and all control calves showed no visible change of the parasitized erythrocytes in the blood during the experimental period. 3. The animals injected over 2 times with 0.2mg/kg body weight tended to be decreased the number of erythrocytes, hemoglobin concentration and PCV for 2-3 days after first injection and continued about one week. 4. All animals injected the dexamethasone were observed the neutrophilic leukocytosis, eosinopenea and lymphopenea until 48 hours after injection.

  • PDF

Immunogenicity and Protective Efficacy of Solubilized Merozoite-enriched Theileria sergenti Immunogens I: Protection against Homologous Stabilate Challenge (Theileriu sergenti merozoite 수용성 항원의 항원성과 면역성)

  • 백병걸;최인혁
    • The Korean Journal of Parasitology
    • /
    • v.30 no.2
    • /
    • pp.133-140
    • /
    • 1992
  • Theileria sergenti were isolated from infected erythrocytes by hypotonic Iysis, and soluble meroBoite antigens were purified by sonication and differential centrifugation. The preparation contained 29, 34, 35 and 105 kD immuno-dominant polypeptides. The soluble antigens (0.5 mg/ml) were prepared and fortified with Freund's adjuvant. Five month old naive Korean calves were subcutaneously inoculated with the preparation and a booster dose was administered 4 weeks later Nine weeks after the booster dose, vaccinates and controls were challenged with a homologous stabilate (5.6×106 RBC/dose, 40% Parasitemia). All animals were monitored for hematocrit, total erythrocyte count, parasitemia and for the specific antibody by Western immuno- blot (WB) and indirect immuno-auorescent antibody(IFA) test. By 18 weeks after vaccination (6 weeks after the challenge), vaccinated cattle had an average IFA titer of 1 : 10,240 compared with 1 : 1,280 of the controls. The vaccinates showed ne91igib1e change in hematocrit and total RBC count whereas control animals showed significant (P<0.05) hematological chanties and associated anemia. After vaccination and challenge, the antibody responses demonstrated that vaccination had induced significant production of antibody to the 29 and 35 kD polypeptides. The latter polypeptide was much more strongly recognized by the vaccinated animals, and thus it may be a potential candidate for the vaccine.

  • PDF

Studies on the effects of immunization against Babesia gibsoni antigen and Theileria sergenti as a non-specific antigen in dog (Babesia gibsoni 항원접종과 Theileria sergenti를 비특이 항원으로 접종한 개의 면역효과에 관한 연구)

  • Yoon, Chang-mo;Lee, Joo-mook;Chae, Joon-seok;Kwon, Oh-deog
    • Korean Journal of Veterinary Research
    • /
    • v.33 no.1
    • /
    • pp.101-108
    • /
    • 1993
  • To examine the effects of vaccination against Babesia gibsoni(B gibsoni) infection in dogs, 15 normal mixed-breed dogs(5 months to 1 year old) were divided into 3 groups with 5 dogs in each group. One of them was selected as control group(group A) and other were selected as experimental groups(group B and C). The group B was vaccinated with antigen which were mixed 0.2% of formalin treated B gibsoni and sonicated one. The group C was inoculated Theileria sergenti as a non-specific antigen. The results obtained in this experiment were summarized as follows; 1. After first vaccination, antibody titers of group B and C were increased 5 times(1:200) than those of control group(1 : 40). The antibody titers of group C were increased more than that of group B after second vaccination. When challenged with the living protozoa(Babesia gibsoni), the antibody titers of C group were elevated higher than that of B group and maintained steadly. Those were not exceeded over 1 : 5,000 for 4 weeks in all 3 groups. 2. After challenge, the peak time of the parasitemia appeared nearly on the 15th day(12~18 days) in all groups. During this period, the rate of parasitized erythrocytes in control group was $55.0{\pm}5.4$‰. But that of group B and C were $41.3{\pm}38.8$‰ and $15.2{\pm}16.3$‰, respectively. 3. After challenge with B gibsoni, all of the values of PCV, Hb, RBC and total leukocytes counts were decreased in both of the experimental and the control. 4. In all groups, there were increased lymphocytes and monocytes after challenge with the protozoa.

  • PDF

Effect of bovine theileriosis on the growth hormone and insulin-like growth factor-I (소의 theileriosis가 성장 hormone과 insulin-like growth factor-I에 미치는 영향)

  • Baek, Byeong-kirl;Byoun, Sun-youn;Lee, John-wha;Lee, Ho-ill
    • Korean Journal of Veterinary Research
    • /
    • v.37 no.2
    • /
    • pp.409-416
    • /
    • 1997
  • Bovine theileriosis caused by Theileria sergenti is the tick-borne intraery- throcytic piroplasmosis, that occurs in most regions of Korea. It results in severe economic losses on a farm caused by anemia, milk production loss, abortion and death. This study was undertaken to confirm the effects of the growth hormone and the insulin-like growth factor-I which are associated in the growth of cattle infected by T sergenti. The blood of one hundred and twenty ten-month Holstein was collected and the prepared blood smear was stained with acridine orange to investigate their parasitemia. And the hematological profiles were observed. According to the value of the hematocrit, they were categorized into four groups : Group 1 was under 20 percent, groups 2 and 3 were from over 21 to under 30 percent and from over 31 to under 35 percent and group 4 was over 36 percent. As the value of the hematocrit decreased, parasitemia(%) in erythrocytes was observed to increase(Y=-1.064X + 30.537, r=0.660). The amounts of the growth hormone and the insulin-like growth factor-I in the serum were measured by the radioimmunoassay. The growth hormone in serum of the group 1, group 2, group 3 and group 4 were observed as $0.238{\pm}0.043nmol/l$, $0.21{\pm}0.024nmol/l$, $0.366{\pm}0.035nmol/l$ and $0.646{\pm}0.223nmol/l$, respectively. The quantitative of the insulin-like growth factor-I in the same groups were observed also as $209.686{\pm}18.94ng/ml$, $250.9{\pm}12.609ng/ml$, $279.3{\pm}8.883ng/ml$ and $365.9{\pm}22.45ng/ml$, respectively. It can be concluded that the growth hormone and the insulin-like growth factor-I were observed to decrease in severe anemia due to theileriosis.

  • PDF

Studies on Incidence of Tick - borne Diseases in Imported Cattle in Korea (도입우에 대한 진드기매개질병의 감염실태에 관한 조사연구)

  • Suh M. D.;Kim Y. H.;Kang Y. B.;Kang S. W.
    • Journal of the korean veterinary medical association
    • /
    • v.18 no.4
    • /
    • pp.19-32
    • /
    • 1982
  • To confirm the etiological agents of tick- borne diseases in the exotic cattle, both dairy and beef cattle, which were newly imported and their progeny expoued at the pasture for grazing, an observation on the incidence and prevalence of blood parasites i

  • PDF

Identical small subunit ribosomal RNA gene nucleotide sequence of bovine Theileria isolates (Korea and Japan) and Theileria buffeli (Marula, Kenya) (한국파 일본의 소에서 분리한 Theileria 분리주와 Theiferia buffeli (Marula, Kenya)의 small subunit ribosomal RNA 유전자 염기서열의 일치)

  • 채준석;권오덕
    • The Korean Journal of Parasitology
    • /
    • v.36 no.1
    • /
    • pp.47-54
    • /
    • 1998
  • Small subunit ribosomal RNA (SSU rRNA) gene nucleotide sequences of bovine ReiLerin isolates from Korea (KLS and KCB) and japan (JHS) were determined. The genes from each isolate were amplified by the polymerase chain reaction and the approxi- mately 1.8 kb product cloned and sequenced by a modified dideoxynucleotide method. Overlapping gene segments produced with a series of primers were sequenced, resoRting in a complete DNA sequence for both forward and reverse strands of the SSU rRNA genes of each isolate. SSU rRNA gene sequences (termed Type A) were identical among the bovine ReiLeri,n isolates from Korea and the isolate from Japan. A GenBank data library homolo- gy search showed the sequence to be the same as that listed as leiLeyia buKeLi isolated from cattle in Marula, Kenya.

  • PDF

Epizootiological survey of blood parasites in slaughtered cattle of western area of Kyeongnam (서부 경남지역의 도살축우에 대한 주혈기생충의 역학적 조사)

  • Jang, Dong-hwa;Suh, Myung-deuk
    • Korean Journal of Veterinary Research
    • /
    • v.30 no.4
    • /
    • pp.473-478
    • /
    • 1990
  • This survey was conducted to observe the prevalence of blood parasites in slaughtered cattle included Korean native cattle, Charolias, Hereford, Aberdeenangus and Holstein breeds in the Western area of Kyeongnam. The results obtained are summarized as follows: 1. The prevalence of T sergenti was shown 71.8% as 395 heads of a total of 550 heads examined and from Jaunary to November the monthly prevalence of T sergenti was shown the range of 61.1% to 84% except 38.5% in December. The other blood parasites included Babesia and Anaplasma were not detected from the blood samples except Setaria spp microfilariae. 2. The distribution of parasitaemia levels of T sergenti in positive cattle was shown 93.9% in the range of 1~10/1000 rbc, 4.1% in 11~20, 1.3% in 21~30 and 0.8% above the range of 31. 3. The pervalence of T sergenti by breeds of slaughtered cattle was shown 71.2% in Korean native cattle, 72.7% in Charolias, 78.3% in Hereford and 81. 8% in others (Aberdeen-angus and Holstein) respectively. Also the parasitaemia levels in these cattle were shown higher levels in imported cattle included Charolias, Hereford, Aberdeen-angus and Holstein breeds comparing with Korean native cattle. 4. The prevalence of Setaria spp microfilariae in slaughtered cattle was shown 6.9% and by monthly prevalence of the parasite was shown higher in March, April and May compared with June, July, August and October. But in the winter season included January, February, November and December the parasite was not detected from the blood samples. 5. The distribution of parasitaemia levels of Setaria spp microfilariae per ml of blood was shown 65.8% in the range of 1~50, 13.2% in 51~100 and 10.5% in 101~200 and above the range of 201, respectively.

  • PDF

A survey for tick-borne pathogens in Korean native cattle from northern area of Gyeongbuk (경북 북부지역의 한우에 대한 진드기매개성 병원체의 감염실태 조사)

  • Cho, Jae-Cheong;Jeon, Woo-Jin;Kim, Seon-Soo;Kim, Seong-Guk
    • Korean Journal of Veterinary Service
    • /
    • v.39 no.1
    • /
    • pp.29-34
    • /
    • 2016
  • This study was carried out to investigate the tick-borne pathogens from one hundred nineteen cattle farms (38 farms of Andong, 41 of Yeongju, 12 of Uiseong, 5 of Cheongsong, 5 of Yoengyang, 18 of Bonghwa) in northern areas of Gyeongbuk province by polymerase chain reaction (PCR). Among 119 cattle farms, the positive ratios against Babesia, Theileria, Anaplasma, Ehrlichia and Rickettsia were 3.4% (4/119), 10.1% (12/119), 6.7% (8/119), 1.7% (2/119) and 16.8% (20/119), respectively. Also, the PCR results revealed that 8 farms were positive for T. sergenti in positive of Theileria and 2 farms were positive for A. phagocytophilum in positive of Anaplasma. Therefore, further studies regarding vectors, environmental condition, interaction between domestic and wild animals and development of control program are needed to reduce the numbers of bovine tick-borne disease in northern areas of Gyeongbuk province.