• The Korean Journal of Mycology
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• v.38 no.1
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• pp.1-4
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• 2010

Orchid mycorrhizal fungi(OMF) were examined in roots of the two threatened orchid species, Cypripedium japonicum and C. macranthum. The morphological characteristics of mycorrhizal colonization in the roots of two orchid species were observed. OMF colonized in the roots of two species were identified using molecular analysis. DNA from the root was extracted and amplified internal transcribed spacer(ITS) region using basidiomycete ITS primers, ITS1-OF and ITS4-OF. Four species belonging to Tulasnellaceae in roots of C. japonicum and two species of Tulasnellaceae and one basidiomycetous species was found in roots of C. macranthum.

• The Korean Journal of Mycology
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• v.39 no.1
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• pp.7-10
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• 2011

Orchid mycorrhizal fungi (OMF) were examined in roots of the six terrestrial species of orchids collected in Ulleung Islands. Seven OMF isolates from the roots of orchids were identified based on morphological and molecular characters. Internal transcribed spacer region of OMF DNA was amplified using basidiomycete-specific ITS primers, ITS1-OF and ITS4-OF. OMF beloning to Tulasnellaceae and Ceratobasidaceae was identified through molecular analysis.

• The Korean Journal of Mycology
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• v.40 no.3
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• pp.132-135
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• 2012

This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.630-636
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• 2010

A survey of the endangered orchid Orchis militaris populations was carried out in north-eastern Italy. The occurrence of fungal root endophytes was investigated by light and electron microscopies and molecular techniques. Two main sites of presence were individuated in the Euganean Hills, differing as to the percentage of flowering individuals and of capsules completing maturity. Fluorescence microscopy revealed an intracellular cortical colonization by hyphal pelotons. Two ITS PCR products co-amplified. Sequencing revealed for the former an identity and a high similarity (99%) with a Tulasnellaceae (Basidiomycota) fungus found within tissues of the same host in independent studies in Hungary and Estonia, suggesting an interesting case of tight specificity throughout the Eurosiberian home range. The second amplicon had 99% similarity with Tetracladium species (Ascomycota) recently demonstrated as potential endophytes. TEM revealed two different hyphal structures. Double fungal colonization appears to occur in Orchis militaris and the possible requirement of a specific fungal partner throws light on the causes of this plant's rarity and threatened status.