• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.9
• /
• pp.4435-4439
• /
• 2012

Purpose: This study aimed to explore the role of the Twist gene in the epithelial-mesenchymal transition of ovarian cancer. Methods: An RNA interference plasmid expressing a small interfering RNA (siRNA)-targeting Twist (Twist siRNA vector) was designed, constructed, and transfected into the human ovarian cancer cell line A2780. Transfection efficiency was assessed under a fluorescence microscope. Changes in the expression of Twist mRNA in A2780 after transfection with the pGenesil Twist shRNA plasmid were analyzed through RT-PCR. MTT assays and adhesion experiments were applied to determine changes in proliferation and adhesion ability of A2870 after transfection with the Twist shRNA plasmid. Changes in the expression of the E-cadherin and N-cadherin proteins in A2780 after transfection with the Twist shRNA plasmid were analyzed using Western blotting. Result: The restructuring plasmid pGenesil-Twist shRNA was constructed successfully. After 48 h of culture, 80% of the cells expressed high-intensity GFP fluorescence and stability. The expression of Twist decreased significantly after the transfection of the Twist shRNA plasmid (P<0.05). Proliferation of the transfected Twist shRNA cells showed no difference with that of the A2780-nontransfection or A2780-si-control groups (P>0.05) but the adhesion ability of A2780 decreased dramatically (P<0.05). Expression of the E-cadherin protein increased, whereas that of the N-cadherin protein decreased compared with that in the A2780-nontransfection or A2780-si-control groups (P<0.05). Conclusion: Twist is essential for epithelial-mesenchymal transition, invasion, and metastasis of ovarian cancer.

• Fibers and Polymers
• /
• v.7 no.1
• /
• pp.79-84
• /
• 2006

This paper deals with the grey fabric deformation during relaxation. It investigates the effect of twist direction and twist liveliness on the 2/2 twill weave woven fabric distortion, during relaxation This experimental work shows that twist liveliness increases the natural tendency of the twill weave woven fabric to skew. In addition when the twist direction of the warp and weft is opposite to each other the bias curling occurs too.

• Proceedings of the KSME Conference
• /
• 2004.11a
• /
• pp.934-939
• /
• 2004

This paper propose a study on the Feed Characteristics of Twist Friction Driver. We are using Twist Friction Driving mechanism system. The system consists of Twist Friction Driver elements such as driving shaft, driven roller, Spring for pre-load, Air bearing guide, Servo motor, and measuring devices such as Encoder of Servo motor, Laser interferometer, LVDT . The Twist Friction driver is mechanically simple and very quiet at high speed, and has low pre-load. So The Twist Friction driver can materialize an ultra precision feed-resolution. The feed characteristics of the driver is determined by slip and angular error, backlash.

• Textile Coloration and Finishing
• /
• v.23 no.1
• /
• pp.35-42
• /
• 2011

For high quality DTP products, it is important to optimize the parameters of media, pre- and after-treatment, ink, printer, etc. This study investigated the effect of types of fabrics(media) as a DTP parameters. Especially, the effects of media properties such as yarn twist and knitted fabric structure, on printability and color difference were examined. Two types of cotton yarn twist(830 and 1630 twist/meter) and five knitted structures of media were prepared with a single circular knitting machine. The K/S values of hard-twist samples were higher than those of normal-twist samples in every media structures. It is more effective to use the knitted fabrics of a hard-twist yarn to obtain dark color in the printing above input level value 60 where the printability improvement was most pronounced in case of plain structure. Among the five media structures a plain structure was the highest and that of corduroy was the lowest in terms of K/S values. Also ${\Delta}E$ values and lightness of the hard-twist yarn samples were smaller than that of normal-twist yarn samples when ${\Delta}E$ values were tested by using a standard of a normal twist yarn sample with a plain structure, which was increased in the case of corduroy structure.

• 한국전산유체공학회:학술대회논문집
• /
• 2010.05a
• /
• pp.67-70
• /
• 2010

Spandex yarn requires a twisting process during winding and unwinding processes at the textile industry. The air-twist nozzle is widely used as part of the winding and unwinding. This paper describes computational approach to design the geometry of the air-twist nozzle. The nozzle has circular yarn-channel and the air-inlet which is perpendicularly connected to the yarn-channel with yarn-loading slit. The air-inlet of the nozzle is designed while measurements of the yarn-channel are fixed. The airflow inside the air-twist nozzle is simulated by using Computational Fluid Dynamic model. The Ansys CFX was used to perform steady simulations of the airflow for the air-twisting process. The vortical structure and the airflow pattern such as velocity streamline, vorticity, velocity of the air-twist nozzle are discussed. Computational results are compared with experimental results in this paper.

• Journal of the Korean Mathematical Society
• /
• v.37 no.3
• /
• pp.491-502
• /
• 2000

The disjoint union of mapping class groups g,1 gives us a braided monoidal category so that it gives rise to a double loop space structure. We show that there exists a natural twist in this category, so it gives us a ribbon category. We show that there exists a natural twist in this category, so it gives us a ribbon category. We explicitly express this structure by showing how the twist acts on the fundamental group of the surface Sg,l. We also make an explicit description of this structure in terms of the standard Dehn twists, as well as in terms of Wajnryb's Dehn twists. We show that the inverse of the twist g for the genus g equals the Dehn twist along the fixed boundary of the surface Sg,l.

• Proceedings of the KSME Conference
• /
• 2004.04a
• /
• pp.917-922
• /
• 2004

As the tasks of robots become more diverse, some complicated tasks have come to require force and position hybrid control. A compliant device can be used to control force and position simultaneously by separating the twist of the robot's end effector from the twist of compliance and freedom by using stiffness mapping of the compliant device. The development of a fuzzy gain scheduling scheme of control for a robot with a compliant device is described in this paper. Fuzzy rules and reasoning are performed on-line to determine the gain of twists based on wrench error and twist error and twist of compliance and twist of freedom ratio. Simulation results demonstrate that better control performance can be achieved in comparison with constant gain control.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.19 no.2
• /
• pp.185-188
• /
• 2006

We studied on the reverse twist near the pixel edge depending on the rubbing direction for the fringe field switching (FFS) mode. Liquid crystal (LC) dynamic and the transmittance near the pixel edge, where the various field directions are generated, depend on the initial rubbing direction because the position of reverse twist is decided by the angle between the electric direction and the LC director at a bias voltage. For example, when the rubbing angle is $7^{\circ}$, the reverse twist appears on the bottom position of the right sharp comer of the pixel edge so that the reverse region exists far away from main active region. But, when the rubbing angle is $-7^{\circ}$, the reverse twist appears on the top position of the right sharp comer of the pixel edge, resulting that the region becomes more close to the main active area and the unstable disclination lines (DLs) easily intrude into the active region. Therefore, it is necessary to keep the reverse twist region far from the active region and it is possible by controlling the rubbing direction in the design of a pixel electrode.

• Journal of dental hygiene science
• /
• v.17 no.5
• /
• pp.433-438
• /
• 2017

Relative to its incidence, oral cancer has serious negative social effects. The exact causes of oral cancer have not been clarified, but many studies have implicated smoking and drinking. However, the fundamental mechanism of oral cancer causation has yet to be elucidated. Lysophosphatidic acid (LPA) augments epithelial mesenchymal transition (EMT) and development of various cancer cells. However, a detailed mechanistic explanation for LPA-induced EMT and the effects of EMT-promoting conditions on oral squamous cell carcinoma development remain elusive. In the present study, a quantitative reverse transcription polymerase chain reaction was used to analyze TWIST1, Slug, E-cadherin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) transcript expression. Immunoblotting was used to analyze TWIST1, Slug, E-cadherin, and GAPDH protein expression. siRNAs were used to silence TWIST1 and Slug transcript expression. A matrigel-coated in vitro invasion insert was used to analyze oral cancer cell invasion. The results of the present study show that the expression levels of TWIST1 and Slug, which are EMT factors, were increased by LPA treatment in YD-10B oral squamous cell carcinoma. Conversely, E-cadherin expression was significantly reduced. In addition, transfection of the cells with TWIST1 and Slug siRNA strongly inhibited LPA-induced oral cancer cell invasion. The present study shows that TWIST1 and Slug mediate LPA-induced oral cancer cell EMT and invasiveness. The present study confirmed the mechanism by which LPA promotes oral cancer cell development, with TWIST1 and Slug providing novel biomarkers and promising therapeutic targets for oral cancer cell development.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.18 no.11
• /
• pp.1033-1037
• /
• 2005

We have studied on the effect of the operation voltage according to the reverse twist for the different fringe field switching (FFS) structure. The FFS structure with a vertically patterned edge of the pixel electrode (VPP) has lower operation voltage comparing to the one with a horizontally patterned edge of the pixel electrode (HPP). The reason is like that the number of the pattern of the pixel edge for the VPP structure is one third comparing with the HPP structure and thus, there is small reverse twist area for the VPP structure. Actually, the reverse twist disturbs the twist of LC near adjacent active area, result that LCs near there have the unstable dynamics. That is, the operation voltage increases as the reverse twist area increases. Therefore, it is very important to design pixel electrode with a small reverse twist region for the FFS mode.