• Title/Summary/Keyword: VN

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Purification and Characterization of Vitellin from the Red Flour Beetle, Tribolium castaneum Herbst

  • Kim, Seong-Ryul;Choo, Young-Moo;Lee, Seong-Jin;Jin, Byung-Rae;Kim, Jeong-Ho;Heo, In-Bum;Shon, Hung-Dae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.1
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    • pp.55-59
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    • 2001
  • The vitellin of the red flour beetled Tribolium castaneum Herbst was purified and characterized. The vitellin of T. castaneum was purified by the FPLC techniques, anion exchange chromatography and gel permeation chromatography. In native-polyacrylamide gel electrophoresis, vitellin of T. castaneum was detected as a single band. This native vitellin has molecular weight of 440 kDa. The vitellin of T. castaneum is composed of three polypeptides, designated Vnl (178 kDa), Vn2 (168 kDa) and Vn3 (52 kDa) in SDS-polyacrylamide gel electrophoresis. Three subunits of vitellin were presented in the female adult hemolymph and egg extracts, but not observed in the male. These three polypeptides gradually decreased during embryogenesis. Polyclonal antiserum raised against purified vitellin reacted with the three polypeptides, Vnl, Vn2 and Vn3. Antisera raised against Vn1 and Vn2 cross-reacted with the two large subunits, Vnl and Vn2, respectively. Another subunits Vn3, however, was not cross-reacted with these two antisera. Also, antiserum raised against Vn3 did not cross-react with the Vn1 and Vn2.

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Microstructure, Crystal Structure and Mechanical Properties of VN Coatings Using Asymmetric Bipolar Pulsed dc Sputtering (비대칭 바이폴라 펄스 스퍼터법으로 증착된 VN 코팅막의 미세구조, 결정구조 및 기계적 특성에 관한 연구)

  • Chun, Sung-Yong;Jeong, Pyeong-Geun
    • Journal of the Korean institute of surface engineering
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    • v.49 no.5
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    • pp.461-466
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    • 2016
  • Nanocrystalline vanadium nitride (VN) coatings were deposited using asymmetric bipolar pulsed dc sputtering to further understand the influence of the pulsed plasmas on the crystal structure, microstructure and mechanical properties. Properties of VN coatings were investigated with FE-SEM, XRD and nanoindentation. The results show that, with the increasing pulse frequency and decreasing duty cycle, the coating morphology changed from a porous columnar to a dense structure, with finer grains. Asymmetric bipolar pulsed dc sputtered VN coatings showed higher hardness, elastic modulus and residual compressive stress than dc sputtered VN coatings. The results suggest that asymmetric bipolar pulsed dc sputtering technique is very beneficial for the reactive sputtering deposition of VN coatings.

Antidiabetic Activity of a Chloroform Fraction of Vigna nakashimae in db/db mice (db/db 마우스 동물모델에서 Vigna nakashimae 클로로포름층 분획물의 항당뇨 효능)

  • Nam, Jeong-Su;Ha, Tae Joung;Park, Jae Hong;Jung, Myeong Ho
    • Journal of Life Science
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    • v.23 no.4
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    • pp.578-585
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    • 2013
  • In this study, we evaluated the antidiabetic effect of a chloroform fraction of a methanol extract of Vigna nakashimae (designated VN) and compared it with that of a water fraction. Both fractions were administrated to eight-week old db/db mice for two weeks, after which the plasma glucose, triglyceride, and total cholesterol levels were measured. The chloroform fraction (VN-C) lowered the fasting glucose and blood glycated hemoglobin in the db/db mice more effectively than those of the water fraction (VN-W). VN-C also improved the glucose tolerance and led to a significant decrease in the plasma levels of free fatty acids and triglycerides. VN-C enhanced the phosphorylation of AMP-activated protein kinase (AMPK) and increased the expression of carnitine palmitoyltransferase-1 (CPT-1) in HepG2 and C2C12 cells more significantly than VN-W. Consistent with AMPK activation, VN-C inhibited cAMP/Dex-stimulated expression of gluconeogenic genes and increased glucose uptake in C2C12. Collectively, these results suggest that VN-C has an antidiabetic effect, which is exerted via AMPK activation, and that this effect is stronger than that of VN-W.

Control of Potato Virus Y (PVY-VN) with Mineral Oil Treatment in Tobacco Burley 21 Fields (담배(Burley 21) 포장에서 mineral oil 처리에 의한 감자바이러스Y(PVY-VN) 방제)

  • 채순용;김상석;김영호;박은경
    • Journal of the Korean Society of Tobacco Science
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    • v.23 no.2
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    • pp.115-122
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    • 2001
  • The effect of mineral oil treatment in Burley 21 tobacco field on the control of potato virus Y(PVY-VN) mostly transmitted by green peach apid(Myzus persicae Sulzer) in nature was studied and the virus infection in some plants including potato, pepper, bramble, radish, etc near the tobacco fields as a virus infection source was tested by capillary tube precipitatioin test with PVY-antibody and bioassay in Xanthi-nc tobacco. The main source of PVY-VN infection in tobacco field in korea was potato(ca. 40% of test plants infected). Pepper and bramble were also infected by PVY-VN. The control level of PVY-VN infection by treatment of 0.75% liquid mineral oil with 3 % nonionic emulsifier to the plants was 84.8 % in case of the artificial transfection with a infected apterous aphid in laboratory. However, the reduction of PVY-VN disease severity in tobacco fields treated with mineral oil at late June was only 35.5%. These results suggest that mineral oil treatment is not so effective for the protection of aphid-born virus(PVY - VN) infection in tobacco fields.

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Regional Comparison of Home Visiting Care Service Satisfaction among Elder Beneficiaries of the Long Term Care Insurance (장기요양보험 등급판정 대상자의 지역별 방문간호 서비스 이용 만족도 및 요구도: 대도시, 중소도시, 농촌 지역을 중심으로)

  • Kwak, Chanyeong
    • Research in Community and Public Health Nursing
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    • v.27 no.2
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    • pp.114-123
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    • 2016
  • Purpose: This study aims at comparing levels of service satisfaction with and needs for visiting nurse(VN) services among their beneficiaries in order to make a plan for extending VN services. Methods: The number of the subjects of this study is 270 in total. They were selected in random sampling in which 30 VN service recipients were randomly selected from each of 7 regions based on their grades and region types. Results: The highest total score for satisfaction with the VN services was 46.30, which the rural residents gave to their received VN services, then the metropolitan residents gave 44.57, and the small and middle size cities' residents 43.64. On the other hand, the highest total score for needs for VN services was 33.03, which the small and middle size cities' residents gave to their received VN services, then the rural residents gave 31.68, and the metropolitan residents 30.88. Conclusion: As the small and middle size cities' residents showed low satisfaction with and high needs for local VN services, policies need to be made to promote service providers' expertise and accessibility.

Properties of VN Coatings Deposited by ICP Assisted Sputtering: Effect of ICP Power

  • Chun, Sung-Yong
    • Journal of the Korean Ceramic Society
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    • v.54 no.1
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    • pp.38-42
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    • 2017
  • Vanadium nitride (VN) coatings were deposited using inductively coupled plasma (ICP) assisted sputtering at different ICP powers. Microstructural, crystallographic and mechanical characterizations were performed by FE-SEM, AFM, XRD and nanoindentation. The results show that ICP has significant effects on coating's microstructure, structural and mechanical properties of VN coatings. With an increase in ICP power, coating microstructure evolved from a porous columnar structure to a highly dense one. Single- phase cubic (FCC) VN coatings with different preferential orientations and residual stresses were obtained as a function of ICP power. Average crystal grain sizes of single phase cubic (FCC) VN coatings were decreased from 10.1 nm to 4.0 nm with an increase in ICP power. The maximum hardness of 28.2 GPa was obtained for the coatings deposited at ICP power of 200 W. The smoothest surface morphology with Ra roughness of 1.7 nm was obtained in the VN coating sputtered at ICP power of 200 W.

Effects of Visiting Nursing Services in Long-term Care Insurance on Utilization of Health Care (노인장기요양보험의 방문간호 서비스가 의료이용에 미치는 효과)

  • Lee, Sangjin;Kwak, Chanyeong
    • Research in Community and Public Health Nursing
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    • v.27 no.3
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    • pp.272-283
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    • 2016
  • Purpose: This is a comparative study using secondary data from the Korean national long term care (LTC) insurance. Methods: Visiting nursing (VN) service users (n=666) and non-users (n=4,375) were extracted and compared in terms of medical expenditures, length of hospitalization, and annual number of ambulatory care visits to investigate effects of VN services in LTC. Results: Total health care expenditures were compared between the two groups and it was found that VN service users spent about $ 1700 than non-users for their medical costs between 2009 and 2011. The average length of in-hospital stay for VN service users was 19.4 days shorter than that of non-users. However, using VN services did not significantly influence the annual number of ambulatory care visits. Conclusion: The study has found that VN services are effective ways of providing community-based LTC services. We recommend LTC policy makers to further utilize VN services to deliver cost effective health care services.

Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency

  • Park, Mi-Ja;Joh, Seong-Joon;Choi, Kang-Seuk;Kim, Aeran;Seo, Min-Goo;Song, Jae-Young;Yun, Seon-Jong
    • Korean Journal of Veterinary Research
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    • v.56 no.3
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    • pp.189-192
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    • 2016
  • The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The $r^2$ values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 $log_{10}$ and HI titer of 5 $log_2$ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.

Effect of Inductively Coupled Plasma on the Microstructure, Structure and Mechanical Properties of VN Coatings (유도결합 플라즈마 파워가 VN 코팅막의 미세구조, 결정구조 및 기계적 특성에 미치는 영향에 관한 연구)

  • Chun, Sung Yong;Lee, So Yeon
    • Journal of the Korean institute of surface engineering
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    • v.49 no.4
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    • pp.376-381
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    • 2016
  • The effects of ICP (Inductively Coupled Plasma) power, ranging from 0 to 200 W, on the crystal structure, microstructure, surface roughness and mechanical properties of magnetron sputtered VN coatings were systematically investigated with FE-SEM, AFM, XRD and nanoindentation. The results show that ICP power has a significant influence on coating microstructure and mechanical properties of VN coatings. With the increasing of ICP power, coating microstructure evolves from a porous columnar structure to a highly dense one. Average crystal grain size of single phase cubic fcc VN coatings was decreased from 10.1 nm to 4.0 nm with increase of ICP power. The maximum hardness of 28.2 GPa was obtained for the coatings deposited at ICP power of 200 W. The smoothest surface morphology with Ra roughness of 1.7 nm was obtained from the VN coating sputtered at ICP power of 200 W.

Effectiveness of Antagonistic Bacterial Metabolites to Control Rhizoctonia solani on Lettuces and Fusarium oxysporum on Tomatoes

  • Vu, Van Hanh;Thi, Quyen Dinh;Rita, Grosch;Dung, Nguyen Ngoc
    • Microbiology and Biotechnology Letters
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    • v.41 no.1
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    • pp.70-78
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    • 2013
  • Rhizoctonia solani and Fusarium oxysporum cause yield losses in numerous economically important crops. To develop a bio-control agent, cell free extracellular compounds (ECs) of 5 bacterial strains Burkholdria sp. L1, Pseudomonas sp. L4, Pseudomonas chlororaphis VN391, Bacillus subtilis VN21 and Enterobacter sp. VN99 from Vietnamese fields, which reduced levels of R. solani root rot in lettuces and F. oxysporum root rot in tomatoes, were investigated. In a growth chamber, ECs of all antagonists markedly enhanced the biomass of lettuces (10 to 14.1%) and tomatoes (11.38 to 13.88%). In greenhouses, the disease's severity on both crops treated with ECs of the antagonists was reduced significantly and biomass losses in the plants decreased markedly. The reduction level of R. solani root rot in lettuces was 75, 66.7, 50, and 16.7% by ECs of strains L1, L4, VN21 and VN391, respectively. The biomass of lettuces increased markedly by 29.13%, 21.67%, and 23.4% by ECs of strains L1, L4 and VN21, respectively. Similarly, the reduction levels of F. oxysporum root rot in tomatoes was 76.3, 75, 41.7 and 25% by ECs of strain L1, L4, VN21 and VN391, respectively, and the biomass was significantly enhanced by 14.42, 12.7 and 13%, respectively. The ECs of strain L1 exhibited the most effective bio-control agents to suppress R. solani and F. oxysporum.