• Title/Summary/Keyword: WAK

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Changes in the Contents of Phenolic Components in the Stem of Acanthopanax koreanum Depending on Extracting Batches (섬오가피의 추출 조건에 따른 페놀성 성분 함량 변화)

  • Kim, Sung Gi;Lee, Jae Bum;Cho, Soon Hyun;Ko, Sung Kwon
    • Korean Journal of Pharmacognosy
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    • v.50 no.2
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    • pp.124-132
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    • 2019
  • This study compared the contents of phenolic components depending on the extracting conditions of Acanthopanax koreanum stem to provide basic information for developing Acanthopanax koreanum-based functional foods. Our findings show that the content of total phenolic component peaked at 16 hours of extraction (WAK-16, 7.22%) and when water extracted at $100^{\circ}C$. However, 11-hours water extraction (WAK-11) showed highest eleutheroside B concentration of 1.32%, a main component of A. koreanum, and the level of chlorogenic acid concentration was the highest when 1-hour water extraction (WAK-1) was conducted, being 2.12%. Moreover, highest concentration of eleutheroside E was observed in 16-hours water extraction (WAK-16) as 1.49%. With 60-hours water extraction (WAK-60), the content of syringaresinol, an active phenolic aglycon substance, concentration was the highest with the value of 0.10%. Isofraxidin showed the highest concentration of water extract(0.09%) for 20 hours (WAK-20) and 0.42% for sinapyl alcohol[16 hours (WAK-16)].

Application of WAK test to Identify Unstable Concrete Sleeper

  • Ngo, Vu Thanh;Park, Jaehak;Park, Seongbaek;Lim, Yujin
    • International Journal of Railway
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    • v.9 no.2
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    • pp.46-49
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    • 2016
  • In this study a new detection technique based on WAK is introduced that can be used for identifying unstable concrete sleeper installed in ballast by triggering activated wave on the sleeper surface. If a gap exists immediately below the concrete sleeper, static stiffness can be lower than stable case's stiffness. The concrete sleeper is assumed as a single degree of freedom system (SDOF). The static stiffness K can be obtained by iteratively calculated mass (m), stiffness (k) and damping coefficient (c) of SDOF system. Those coefficients are used to specify the ballast condition such as a gap between the sleeper and ballast. Typical test results using a small sleeper model test are summarized and explained for proof of effectiveness of the WAK test for checking unstable condition of the sleeper.

Estimation of Reservoir Inflow Using Frequency Analysis (빈도분석에 의한 저수지 유입량 산정)

  • Maeng, Seung-Jin;Hwang, Ju-Ha;Shi, Qiang
    • Journal of The Korean Society of Agricultural Engineers
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    • v.51 no.3
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    • pp.53-62
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    • 2009
  • This study was carried out to select optimal probability distribution based on design accumulated monthly mean inflow from the viewpoint of drought by Gamma (GAM), Generalized extreme value (GEV), Generalized logistic (GLO), Generalized normal (GNO), Generalized pareto (GPA), Gumbel (GUM), Normal (NOR), Pearson type 3 (PT3), Wakeby (WAK) and Kappa (KAP) distributions for the observed accumulative monthly mean inflow of Chungjudam. L-moment ratio was calculated using observed accumulative monthly mean inflow. Parameters of 10 probability distributions were estimated by the method of L-moments with the observed accumulated monthly mean inflow. Design accumulated monthly mean inflows obtained by the method of L-moments using different methods for plotting positions formulas in the 10 probability distributions were compared by relative mean error (RME) and relative absolute error (RAE) respectively. It has shown that the design accumulative monthly mean inflow derived by the method of L-moments using Weibull plotting position formula in WAK and KAP distributions were much closer to those of the observed accumulative monthly mean inflow in comparison with those obtained by the method of L-moment with the different formulas for plotting positions in other distributions from the viewpoint of RME and RAE.

T2 Relaxographic Mapping using 8-echo CPMG MRI Pulse Sequence

  • E-K. Jeong;Lee, S-H.;J-S. Suh;Y-Y wak;S-A. Shin;Y-K. Kwon;Y. Huh
    • Journal of the Korean Magnetic Resonance Society
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    • v.1 no.1
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    • pp.7-20
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    • 1997
  • The mapping of the spin-spin relaxation time T2 in pixed-by-pixel was suggested as a quantitative diagnostic tool in medicine. Although the CPMG pulse sequence has been known to be the best pulse sequence for T2 measurement in physics NMR, the supplied pulse sequence by the manufacture of MRI system was able to obtain the maximum of 4 CPMG images. Eight or more images with different echo time TEs are required to construct a reliable T2 map, so that two or more acquisitions were required, which easily took more than 10 minutes. 4-echo CPMG imaging pulse sequence was modified to generate the maximum of 8 MR images with evenly spaced echo time TEs. In human MR imaging, since patients tend to move at least several pixels between the different acquisitions, 8-echo CPMG imaging sequence reduces the acquisition time and may remove any misregistration of each pixel's signal for the fitting T2. The resultant T2 maps using the theoretically simulated images and using the MR images of the human brain suggested that 8 echo CPMG sequence with short echo spacing such as 17∼20 msec can give the reliable T2 map.

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Comparison of Safety factor for an Anchored Slope in Accordance with the Effects of Load Transfer (앵커 하중전이에 따른 사면의 안전율 비교 연구)

  • Kim, Sung-Kyu;Kim, Wak-Kyung;Park, Jong-Sik;Joo, Yong-Sun;Kim, Tae-Hoon
    • Journal of the Korean Geotechnical Society
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    • v.24 no.11
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    • pp.55-60
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    • 2008
  • This paper presents how the load transfer mechanism of the ground anchor affects on the stability analysis of anchored slope. The finite element analysis and the conventional limit equilibrium analysis on the anchored slope were performed and compared. The limit equilibrium analysis of the anchored slope is widely used in design practice due to the easiness of the analysis. However, the load transfer mechanism is not considered properly for the analysis. When the failure surface passes through the bonded length of an anchor, the anchor load is disregarded and the factor of safety for the anchored slope is smaller than it should be. In this study, the load transfer distribution was incorporated into the limit equilibrium stability analysis of the anchored slope and the results were compared with those of finite element analysis.

Functional characterization of a CCCH type zinc-finger protein gene OsZF2 by ectopic overexpression of the gene in rice (과발현 형질전환벼에서 CCCH type zinc-finger protein 유전자 OsZF2 기능 분석)

  • Lee, Jung-Sook;Yoon, In-Sun;Yoon, Ung-Han;Lee, Gang-Seob;Byun, Myung-Ok;Suh, Seok-Chul
    • Journal of Plant Biotechnology
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    • v.36 no.1
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    • pp.23-29
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    • 2009
  • We have previously isolated a CCCH type zinc-finger protein gene, OsZF2 (Oryza sativa Zinc Finger 2), from the cold-treated rice cDNA library. To investigate the potential role of OsZF2, transgenic rice lines over-expressing OsZF2 under the control of CaMV 35S promoter have been developed through Agrobacterium-mediated transformation. Elevated level of OsZF2 transcripts was confirmed by RNA gel blot analysis in transgenic rice. Under the 100 mM NaCl condition, the transgenic rice showed significantly enhanced growth rate in terms of shoot length and fresh weight, implicating that OsZF2 is likely to be involved in salt response of rice. In the field condition, however, the transgenic rice showed a dwarf phenotype and flowering time was delayed. Genome expression profiling analysis of transgenic plants using the 20K NSF rice oligonucleotide array revealed many up-regulated genes related to stress responses and signaling pathways such as chaperone protein dnaJ 72, salt stress-induced protein, PR protein, disease resistance proteins RPM1 and Cf2/Cf5 disease resistance protein, carbohydrate/ sugar transporter, OsWAK kinase, brassinosteroid LRR receptor kinase, and jasmonate O-methyltransferase. These data suggest that the CCCH type zinc-finger protein OsZF2 is a upstream transcriptional factor regulating growth and stress responsiveness of rice.

Effects of Dietary Fatty Acids on fatty Acid Pattern in Development Rat Brain Phospholipids - Effects on P/M/S and $\omega$3/$\omega$6 Fatty Acid Ratios -

  • Um, Young-Sook;Chung, Eun-Jung;Lee-Kim, Yang-Cha
    • Journal of Nutrition and Health
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    • v.31 no.5
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    • pp.897-905
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    • 1998
  • Docosahexaenoic acid(DHA), a $\omega$3 series fatty acid and arachidonic acid(AA). a $\omega$6 series fatty acid were found in relatively high concentrations in the phospholipids(PLs) of cell membranes of nerve tissues, and they can be affected by various factors. The present study examined the effects of dietary $\omega$6 and $\omega$3 fatty acid composition on P/M/S and on $\omega$3/$\omega$6 fatty acid ratios in brain PLs of 2nd generation rats. The expeimental diets consisted of 10% fat(by wt), which were computer- searched mixed oil('M') with P/M/S ratio, 1 : 1.4 : 1 and $\omega$6/$\omega$3 ratio, 6 : 1 and safflower oil('S') poor in $\omega$3 fatty acids. The experimental diets were started 3-4 wks prior to conception. During the lactation period, the feeding mothers were switched 1 wk after birth and provided the pups for 2 wks with milk which had compositions different from that of their natural mother. The same diet as their mothers was provided from weaning to 9 wks of age. The 'M'and 'S' rats were again subdivided into MM, MS, SS, SM rats according to diet which their lactating mothers were fed from the begining of the experiment. The relative percentage of P/M/S fatty acids in brain PLs in all experimental groups converged to a very similar value at 9 wks of age, indicating the existence of a control mechanism for the degree of fatty acids, unsaturation. The $\omega$3/$\omega$6 fatty acid ratios of brain PLs converged to about 1.0 in MM & SM groups and to 0.7 in SS & MS groups, suggesting also the existence of some balance between $\omega$3 and $\omega$6 fatty acids in developing rat brain. The concentrations of $\omega$3 fatty acids, especially DHA, in the SM group were increased and became similar to those in MM group at 9 wks of age. The increase in DHA of brain PLs was counterbalanced b)r a decrease in 22 5$\omega$6. Therefore, the ratios of 22 : 6$\omega$6/22 : 5$\omega$6 were higher in both MM & SM groups than those of SS & MS groups at 9 wak of age. Although dietary $\omega$3 and $\omega$6 fatty acids affected 22 : 6$\omega$S and 22 : 5$\omega$6 contained in rat brain PLs reciprocally, the relative percentage of AA did not appear to be significantly influenced by the diet in all groups at 9 wks of age, suggesting that a mechanism for the maintenance of a certain level of AA in brain PLs exists. In conclusion, the $\omega$3/$\omega$6 fatty acid and 22 : 6$\omega$3/22 : 5$\omega$6 ratios, but not P/M/S ratio, of rat brain PLs were affected by the postnatal dietary changes. Futher studies are required to clarify the mechanism(S) of ensuring a certain level of DHA and of maintaining a similar level of AA in rat brain PLs after. weaning(9 wk) regardless of prenatal and postnatal dietary changes. (Korean J Nutrition 31(5) : 897-905, 1998)

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