• Title/Summary/Keyword: Yersinia enterocolitica

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Comparison of Biotyping, Serotyping and Molecular Typing of Yersinia enterocolitica Isolated from Spring water in Seoul (서울시내 약수에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 분자학적 형별비교)

  • 이영기;최성민;오수경;신재영
    • Journal of environmental and Sanitary engineering
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    • v.14 no.4
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    • pp.99-109
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    • 1999
  • Enteropathogenic Yersina enterocolitica is an important cause of human and animal disease. Phenotypic and genotypic characteristics currently used to identify Yersinia enterocolitica are not necessarily sufficient to differentiate pathogenic from non-pathogenic strains or to analyze the epidemiology of yersiniae at a molecular level. To improve the characterization of Yersinia enterocolitica, A total of 65 isolates of Yersinia enterocolitica were examined with bioserotyping, antibiotic susceptibilities, PFGE, PCR-ribotyping. Genomic DNA pattern generated by PFGE are highly specific for different strains of an organism and have significant value in epidemiologic investigations. The PFGE analysis of Not I-digested chromosomal DNA of Y. enterocolitica were performed with a CHEF Mapper(Bio-Rad, USA). Not I generated 19 restriction endonuclease digestion profiles(REDP). PCR-ribotyping, performed with primers complementry to conserved regions of 16S and 23S rRNA gene, generated 13 ribotypes. PCR-ribotyping can be considered a good technich for subtyping strains of Y.enterocolitica.

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Serological cross-reaction with Brucella abortus antigen extracted by sodium dodecyl sulfate and Yersinia enterocolitica 0:9 (SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구)

  • Lim, Yoon-kyu;Yang, Ki-chun;Lee, Kyung-kap;Park, Jun-hong;Lee, Du-sik;Park, Yong-ho;Kang, Seung-won;Mok, Ji-won;Lee, Yong-soon
    • Korean Journal of Veterinary Research
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    • v.35 no.1
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    • pp.143-148
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    • 1995
  • Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.

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Biotype, serotype and antibiotic susceptibility of Yersinia enterocolitica isolated from swine (돼지에서 분리한 Yersinia enterocolitica의 생물형, 혈청형 및 항균제 감수성)

  • Park, Seog-gee;Choi, Chul-soon;Jeon, Yun-seong
    • Korean Journal of Veterinary Research
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    • v.32 no.1
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    • pp.63-76
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    • 1992
  • A study on the isolation of Yersinia from the feces of healthy pigs and the biotype and serotype and susceptibility to 16 antimicrobials was carried. Out of 853 pigs, Yersiniae were isolated from 349 pigs(40.9%). Of 349 isolates, 289 isolates(82.8%) were Yersinia enterocolitica and 54(15.5%) were Y kristensenii, 3(0.9%) were Y pesudotuberculosis and the rest 3(0.9%) were Y prederiksenii. Out of 289 isolates of Y enterocolitica, the predominants biotype was 3B comprising of 165 isolates(57.1%) and followed by biotype 2, comprising of 49 isolates(17.0%), bioptype 3A, comprising of 41 isolates(14.2%) and biotype 4 comprising of 23 isolates(8.0%). And the predominant serotype was 0 : 3 comprising of 231 isolates(79.9%) and followed by serotype 0 : 9 comprising of 42 isolates(14.5%) and 0 : 21 comprising of 10 isolates(3.5%). Y. enterocolitica were resistant to cephalothin(99%), novobiocin(99%), erythromycine(83%), ampicillin(83%) and carbenicillin(81%) and susceptible to amikacin(100%), colistin(100%), gentamicin(100%), kanamycin(100%), polymyxin B(100%), tobramycin(100%), chloramphenicol(99%), nalidixic acid(99%), neomycin(99%), streptomycin(99%) and tetracycline(99%). Most strains of biotype 2/serotype 0 : 9 were susceptible to carbenicillin(100%) and ampicillin(61%) but the other biotype/serotypes were resistant to these antibiotic.

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Characteristics of Yersinia enterocolitica isolates from beef and pork carcass (소와 돼지도체에서 Yersinia enterocolitica의 분리 및 특성)

  • Chae, Hee-Sun;Kim, Joo-Young;Kim, Jee-Eun;Yang, Yun-Mo;Jin, Kyung-Sun;Shin, Bang-Woo;Kim, Sun-Heung;Lee, Jung-Hark
    • Korean Journal of Veterinary Service
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    • v.31 no.2
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    • pp.195-205
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    • 2008
  • Yersinia enterocolitica is a zoonotic agent, and to cause food poisoning. This study was carried out to get some basic information for the control of Yersinia infection. A total of 1,680 samples were collected from beef and pork carcasses from January 2006 to December 2007 in Seoul. The isolation rate was higher in pork carcass than in beef carcass. Five (0.59%) Yersinia enterocolitica were isolated from the 840 of beef carcasses, and eighteen(2.14%) were isolated from the 840 of pork carcasses. Among 23 strains, 22 were classified into biotype 1A, and one was biotype 6. In serotyping of Y enterocolitica isolates, 21 strains were untypable (UT), and 2 were O5 and O8 respectively. In PCR, Ail gene was not detected in all of 23 strains that determined non-pathogenic. In antimicrobial susceptibility test, twelve strains (52.2%) of 23 isolates showed the multi -resistant patterns with over 3 drugs. PFGE was performed after the genomic DNA of twenty three isolates, which was digested with Xba I. the 23 isolates showed 12 ($A{\sim}L$) PFGE type.

Novel Pathogenetic Mechanism in a Clinical Isolate of Yersinia enterocolitica KU14

  • Sato Yoshinori;Kaneko Kenichi;Sasahara Takeshi;Inoue Matsuhisa
    • Journal of Microbiology
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    • v.44 no.1
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    • pp.98-105
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    • 2006
  • Yersinia enterocolitica induces a broad range of gastrointestinal syndromes, including acute enteritis. We previously reported that the clinical isolate, Y. enterocolitica KU14, which lacks pYV, was still capable of causing clinical infection. The present study demonstrated that KU14 did not trigger the death of macrophages in vitro, unlike WA-314 (ATCC51871, which harbors the pYV virulence plasmid). However, the intracellular growth of KU14 in the macrophages was greater than that of WA-C (ATCC51872, a non-plasmid harboring the derivative pYV plasmid). Treatment with a cholesterol-binding drug $(\beta-cyclodextrin)$ that affected lipid rafts resulted in a dramatic reduction in the inracellular growth of KU14. These data clearly indicate that the enhanced inracellular growth of KU14 is related to lipid raft-mediated infection.

Growth Characteristics and Plasmid Profiles of Yersinia enterocolitica lsolated from Springs Water (약수터수로부터 분리한 Yersinia enterocolitica의 성장특성 및 Plasmid 유형)

  • 차인호;김미희;이상준
    • Journal of Life Science
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    • v.7 no.3
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    • pp.212-218
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    • 1997
  • The studies were conducted to explore the dffects of growth or survival against various factors and plasmid profiles of 49 Y. enterocolitica isolated from springs water. In the presence of calcium hypochlorite, y. enterocolitica was entirely extinguished by exposure for 33 hours at 0.8 ppm concentration, and was grown up to 7% NaCl, but not at 95 NaCI. Y. enterocolitica was presented optimal growth at pH 7.0 anad 9.0, and not allowth the growth at pH3.0, 5.0 and 11.0. The optimal temperature for growth of Y. enterocolitica was 25$\circ$C and 35$\circ$C, and allowed the growth at refrigerant temperature, 5$\circ$C. Y. enterocolitica was remarkably decreased by exposure for 30 seconds under UV light, and entirely extinguished by exposure for 90 seconds. Therefore, UV light was effective for sterilization of Y. enterocolitica. Fourty-nine strains of Y. enterocolitica were harbor plasmid DNA of approximately 46 Kb molecular weight.

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Detection of Pathogenic Yersinia enterocolitica Strains by a Rapid and Specific Multiplex PCR Assay

  • Kim Young-Sam;Kim Jong-Bae;Eom Yong-Bin
    • Biomedical Science Letters
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    • v.10 no.4
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    • pp.333-339
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    • 2004
  • A multiplex PCR assay targeting the yst and 16S rRNA genes of Yersinia enterocolitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Simultaneous amplification of 145 and 416 bp fragments of the yst and 16S rRNA genes of Y. enterocolitica was obtained using the primer pairs in a single reaction. Validation of the assay was performed with the reference Yersinia strains and other members of the family Enterobacteriaceae. The defined primer pairs amplified the targeted sequence from only pathogenic Y. enterocolitica strains, whereas none of the other bacterial species yielded any amplified fragments. Within an assay time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laboratories to identify pathogenic Y. enterocolitica.

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Comparison of Common Enrichment Methods for Recovery of Yersinia Enterocolitica from Artificially Inoculated Swine Feed Samples

  • Kim, Joo-Sung;Draughon, F.A.
    • Journal of Food Hygiene and Safety
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    • v.25 no.4
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    • pp.320-324
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    • 2010
  • Five different enrichment methods were studied to find an optimal method to recover Yersinia enterocolitica from swine feed samples. When the recovery of Y. enterocolitica GER-C (serotype O:3) strain was studied at 1000 CFU/g feed, phosphate-buffered saline (PBS) enrichment at $4^{\circ}C$ and PBS plus sorbitol and bile salts (PSB) enrichment at $4^{\circ}C$ and $21^{\circ}C$ were not effective (< 22%). In contrast, both irgasan-ticarcillin-potassium chlorate (ITC) and tryptic soy broth plus polymyxin B sulfate and novobiocin (TSBPN) enrichment methods showed a full recovery (100%) at 100-1000 CFU/g feed. At 10 CFU/g feed, both ITC and TSBPN methods still recovered the strain (> 50%). In recovery of ATCC 9610 (serotype O:8) strain, TSBPN method was more sensitive than any other methods (P < 0.05) at 1000 CFU/g feed. Using TSBPN method, the strain was still recovered at 100 CFU/g feed, but not at 10 CFU/g feed. With its sensitivity and relatively simple recipe, TSBPN was most desirable method to recover Y. enterocolitica from swine feed samples.

Predicting the Contamination of Listeria Monocytogenes and Yersinia enterocolitica in Pork Production Using Monte Carlo Simulation (몬테카를로 시뮬레이션을 이용한 돈육 생산공정에서의 Listeria monocytogenes 및 Yersinia enterocolitica의 오염수준 예측)

  • Rho, Min-Jeong;Chung, Myung-Sub;Park, Ji-Yong
    • Korean Journal of Food Science and Technology
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    • v.35 no.5
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    • pp.928-936
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    • 2003
  • Monte Carlo simulation was used to predict the contamination levels of Listeria monocytogenes and Yersinia enterocolitica in final pork products. Mean values of the estimated log contaminated levels of L. monocytogenes on carcasses, cut meats, and cut meats after storage were -4.59, -4.46 and -4.45 $log_{10}CFU/cm^2$ respectively. The mean values of estimated log contaminated levels of Y. enterocolitica on carcasses, cut meats, and cut meats after storage were -3.44, -4.00 and -3.97 $log_{10}CFU/cm^2$, respectively. Sensitivity analysis showed that L. monocytogenes and Y. enterocolitica in pork was most sensitive to the prevalence of L. monocytogenes and Y. enterocolitica in the equipment used.

Prevalence of antibody against 38 kDa outer membrane protein of Yersinia enterocolitica in swine (국내 사육돼지에서의 Yersinia enterocolitica 38 kDa outer membrane protein에 대한 항체가 분포)

  • Shin, Seong-jae;Park, Joo-youn;Choi, In-soo;Shin, Na-ri;Yoo, Han-sang
    • Korean Journal of Veterinary Research
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    • v.41 no.1
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    • pp.73-78
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    • 2001
  • Yersinia enterocolitica is an inhabitant in the lower intestinal tract of many domestic and wild animals as well as in the nature. Of the several forms of diseases caused by Y. enterocolitica, an acute enteritis, especially in young children, is the most common form. Infection of the bacteria usually occurs through fecal-oral route by contaminated foods or water, especially mountainspring water. Of the domestic animals, swine has been known as one of the most important carrier in the human infection. Based on the knowledge, prevalence of antibody against Y enterocolitica was investigated with swine sera collected from Korea for the survey of Y enterocolitica infection in swine. As the first step of this survey, we analyzed outer membrane protein (OMP) profiles of the representative strains of Y enterocolitica isolated from the feces of piglets and mountainspring water in Korea. Thirty-eight kDa OMP was identified as the common OMP regardless of origin, serotype, or biotype of Y enterocolitica isolates. Presence of antibody specific for 38 kDa OMP of Y enterocolitica in 1,076 swine sera collected from November 1999 to October 2000 was analysed with ELISA. Antibody titer in sows was significantly higher than that in piglets, growing pigs and finishing pigs (p<0.05). Also, there was seasonal difference in the prevalence of antibody against Y enterocolitica. These results would provide the basic knowledge for controlling the Y enterocolitica infection in human as well as swine.

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