• Journal of Sasang Constitutional Medicine
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• v.11 no.2
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• pp.283-299
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• 1999

1. Purpose : Systemic injection of kainic acid in experimental animals induces the limbic seizure and structural damages in hippocampus and amygdala which resembles the changes in human temporal lobe epilepsy. The author performed this study to investigate the neuroprotective effects of Yuldahansotang, on the neurotoxicity induced by kainic acid in the hippocampus in rats. 2. Method : Kainic acid was administered intraperitoneally. And feeding with Yuldahansotang for 3 weeks after kainic acid administration. Seizure were induced in male mice (kainate 10-40 mg/kg i.p) and animals were sacrified at various time-points after injection. The experimental animals were sacrificed at 1, 2, 3day and 1, 3weeks while Yuldahansotang administrations. Seizure were graded using a behavioral scale developed in our laboratory. c-fos belong to immediate early genes(IEGs) known to have rapid and brief responses. And neuronal injury was assayed by examining DNA fragmentation using in situ nick translation histochemistry. 3. Results & Conclusion : Seizure severity paralled kainate dosage. At higher doses DNA fragmentation is seen mainly in hippocampus in area CA3, and variable in CA1, thalamus, amygdala within 24 h, is maximal within 72 h, and is large gene by 7 days after administration of kainate. And we can't see the expression of DNA fragmentation and c-fos in the mice what feeded by Yuldahansotang after 7 days from kainic acid administration. It is consequently suggested that Yuldahansotang may attenuate the kainic acid-induced neuronal degeneration and increase the immunoreactivity of hippocampus in mouse.

• Journal of Sasang Constitutional Medicine
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• v.34 no.2
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• pp.75-83
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• 2022

Objectives The aim of this study is to report the improvement in Taeeumin's dryness-heat symptom of a 54-year-old man diagnosed with type-II diabetes mellitus after treated by herbal medicine. Methods The Taeeumin patient took Yuldahansotang-gamibang for 9 months to manage Taeeumin's dryness-heat symptom. We examined the changes of Body Mass Index(BMI), Fating Blood Sugar(FBS), Hemoglobin A1c(HbA1c), and ketouria. Results Type-II diabetes mellitus symptoms in the patient with Taeeumin's dryness-heat symptom were thirst, fatigue, and increased body weight. All numerical levels for blood sugar, HbA1c, and ketouria were reduced after taking Yuldahansotang-gamibang. Conclusions The treatment of the patient with Taeeumin's dryness-heat symptom and type-II diabetes mellitus with Yuldahansotang-gamibang was efficient, and further research is needed to prove the effect of Yuldahansotang-gamibang for treating such patients.

• Journal of Sasang Constitutional Medicine
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• v.10 no.2
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• pp.565-588
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• 1998

1. Background and Purpose : I intended to investigate the effects of Taeumin Yuldahansotang and Jowisokmyungtang experimentally to hypertension and renal function and contrived to approach these diseases by constitutional medicine. 2. Methods : I adapted two groups, normal rats and spontaneously hypertensive rats, to the same environment for more than 2 weeks and adminstrated water extracts of Yuldahansotang and Jowisokmyungtang. After adminstration of water extracts, I observed significant changes of values of the blood pressure and the urinary excretion of creatininine, electrolytes, osmolarity, renin, atrial natriuretic peptide and the plasma level of aldosterone, triglyceride, phospholipid, cholesterol. 3. Results : Yuldahansotang and Jowisokmyungtang decrease the blood pressure in the spontaneous hypertensive rats, the one by increasing the plasma level of atrial natriuretic peptide and decreasing the plasma level of aldosterone, the other by decreasing the plasma level of aldosterone, from which we could detect the therapeutic difference between Yuldahansotang and Jowisokmyungtang.

• Journal of Sasang Constitutional Medicine
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• v.14 no.1
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• pp.79-89
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• 2002

To evaluate the effect of Yuldahansotang(YHT) water extract on cultured hippocampal cell was inhibited by hydrogen peroxide, MTT assay, NR assay, Neurofilament enzymeimmuno assay and DNA synthesis assay were carried out after the cultured hippocampal cells were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of hydrogen peroxide. The results obtained were as follows: 1. Hydrogen Peroxide decreased the survival rate of the cultured hippocampal cells on NR assay and MIT assay. 2. YHT water extract have efficacy of increasing a amount of neurofilament decreased by hydrogen peroxide in cultured hippocampal cells. 3. YHT water extract have efficacy of increasing DNA synthesis decreased by hydrogen peroxide in cultured hippocampal cells. From above the results, It is concluded that YHT has marked efficacy in preventing for the damages by hydrogen peroxide.

• Journal of Sasang Constitutional Medicine
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• v.16 no.1
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• pp.148-154
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• 2004

Abdominal distention is a common condition, which usually results from over-eating, rather than from a serious illness. Abdominal distention is often caused by intestinal gas. Abdominal distention may also occasionally result from the accumulation of fluid in the abdomen, which can be a sign of serious medical problems. This stroke patient has been abdominal distention without medical problems. So he was treated by Yuldahansotang(熱多寒少湯) and was improved not only activity daily life but also Abdominal distention.

• Journal of Sasang Constitutional Medicine
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• v.14 no.2
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• pp.169-174
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• 2002

A 55-year-old woman who was diagnosed as Diabetic foot was admitted to Dong-so Oriental-medical hospital on April 15th, 2002. Her great toe and secondary were to be tut off, and she was much depressed. We diagnosed her constitution as Taeumin(太陰人), and classified her symptom as Taeumin-Ban-Chang-Byung( 太陰人 ?瘡病 ) and prescribed Yuldahansotang(熱多寒少湯) for the purpose of chengganjoyoil(淸肝燥熱). During admission, the ulceration had nearly disappeared and so she could save her pre[ious toes. From this case, even though we could not say that Diabetic foot is equal to Ban-Chang-Byung but could suppose that there was some relationship between one and the other.

• Journal of Sasang Constitutional Medicine
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• v.14 no.3
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• pp.97-113
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• 2002

Objective To study the effectiveness of Yuldahansotang against Allergic Contact Dermatitis, the change of cutaneous shape, histochemistry, immunohistochemistry and distribution of apoptotic cells was researched. materials and methods 4-month-old rats were divided into three groups of 10. One is a contrastive group which has applied Acetone olive oil only. Another is ACD group which has intentionally activated Allergic Contact Dermatitis by DNCB. And the other is YST group which has given medication of Yangkyuksanhawtang extract. Each group of mice were observed after 24, 48 and 72 hours. results 1. With the result of Contact hypersensitivity assay, YST group shows appreciably less ear swelling than ACD group. 2. Comparing YST and ACD groups to each other regarding general change of skin, YST group shows less hyperplasia of epidermis, less migration of inflammatory cells and less damage of epidermis than ACD group. 3. Regarding the change of collagen fiber, ACD group has appeared to be low in number of collagen fiber while YST shows similarity with the contrastive group. 4. In dermis YST group has showed lower number of mastocyte than ACD group and is granulated type. 5. In dermis YST group has showed less MAC-1,IL-1, $IL-2R-{\alpha}$, ICAM-1 and VCAM-1 than ACD group. 6. The distribution of apoptotic cells has appeared littler in YST group than in ACD 7. Among signal molecule of apoptosis Bcl-2 has distributed more in YST group than ACD group and Bax and Fas has distributed less in YST group than ACD group.

• Journal of Sasang Constitutional Medicine
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• v.13 no.1
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• pp.88-96
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• 2001

To evaluate the effect of Yuldahansotang(YHT) water extract on cultuted mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MIT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried our after the cultured mouse spinal sensory neuron were preincubated with various concentrations of YHT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cells on NR assay and MTT assay. 2. MTT50 value and NR50 value pf XO/HX were 20 mU/ml XO/0.2 mM HX and 40 mU/ml XO/0.2 mM HX. 3. YHT water extract have efficacy of increasing neurofilament. 4. YHT water extract have efficacy of increasing LDH activity. From above the results, It is concluded that YHT has marked efficacy as a treatment for the damages caused in the XO/HX-mediated oxidative process.

• Journal of Sasang Constitutional Medicine
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• v.12 no.1
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• pp.201-215
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• 2000

1. Background and Purpose According to Sasang constitutional medicine, Yuldahansotang(YHT) is a useful prescription for Teaumin patients with a variety of neurologic disorders. Then, I investigated about certain relationships between the effect of YHT and the changes of immune system, especially cytokine network. 2. Methods We studied 8 Taeumin patients with Cerbral infarction. They were treated with YHT in constitutional clinic of Wonkwang Kwagnju Oriental Hospital. We investigated the changes of cytokine network of them. We also investigated cytokine release by lipopolysaccharide- activated peripheral blood mononuclear cells from healthy Taeumin controls. 3. Results The mean interleukin (IL)-2 plasma levels were slightly lower in the plasma of patients than in normal group, whereas the mean IL-4, IL-6 and IgE levels were significantly higher. But there were no significant differences in $interferon-{\gamma}$($IFN-{\gamma}$) levels between each group. After administration of YHT for two to four weeks, plasma levels of $IFN-{\gamma}$ and IL-2 derived from T helper (Th) 1 cells were elevated significantly, whereas plasma levels of IL-4 and IL-6 derived from Th2 cells were reduced significantly. Plasma levels of IgE were reduced significantly, too. During the period of YHT administration, other adverse effects are not shown. It is increased significantly to Cytokine release by lipopolysaccharide -activated peripheral blood mononuclear cells from healthy Taeumin controls. And the release of $IFN-{\gamma}$, IL-2 and IL-6 was progressively decreased in the plasma treated with YHT. It shows regulatory effects of YHT to cytokine production.

• Journal of Sasang Constitutional Medicine
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• v.10 no.1
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• pp.285-293
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• 1998

Yuldahansotang(YH) has been used in Sasang(四象) constitution medicine for many years as a therapeutic agent for cerebral disease. The effect of YH on the vascular system is not known. The purpose of this study was to determine the effect of YH on blood pressure, regional cerebral blood flow(rCBF) and pial arterial diameter of rats. 1. Blood pressure decreased by YH in rats. 2. rCBF was increased by YH in a dose-dependent manner. 3. Pretreatment with propranolol, methylene blue and indomethacin significantly inhibited YH induced increase in rCBF. 5. Blood pressure increased by Radix Puerariae(RP) and Radix Ligustici Tenuissimae(RLT) but Radix Scutellariae(RC) decreased blood pressure in rats. 6. rCBF was increased by RP and RLT in a dose-dependent manner but RC decreased low dosage, and RC increased high dosage. 7. Pial arterial diameter was increased by YH in a dose-dependent manner. 8. Pretreatment with propranolol significantly inhibited the increased in pial arterial diameter induced by YH. These results suggest that YH causes a diverse response of blood pressure, regional cerebral blood flow(rCBF) and pial arterial diameter. The increase in rCBF is also mediated by prostaglandins, cyclic GMP and adrenergic ${\beta}$ receptor and the increase in pial arteral diameter is mediated by adrenergic ${\beta}$ receptor.