• Title/Summary/Keyword: aacC2

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Molecular Biological Detection of the Genes Encoding Aminoglycosise Acetyltransferases and Aerolysin in Water Samples from Juam Lake (주암호에서 Aminoglycoside Acetyltransferases와 Aerolysin 유전자의 분자생물학적 검출)

  • 이영종;한효심;정재성
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.273-278
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    • 2000
  • The aacCl, aacC2, aacC3, and aacC4 genes, which encode aminoglycoside acetyltransferase AAC(3)-I, AA(3)-II, AAC(3)-III, and AAC(3)-IV, respectively, and aerolysin genes in water samples from Juam lake were surveyed by polymerase chain reaction. Surface water was collected from January 1996 to December 1998, and then bacterial DNA was extracted from the water. Twelve samples were tested by PCR to servey the genes for aminoglycoside acetyltransferase and aerolysin in the lake water. The aacC2 gene was detected in 9 of 12 DNA samples. Among 9 samples showing aacC2 positive, 7 samples were associated with Tn3 sequence. However, none of the twelve samples amplified the expected DNA fragment for aacC1, aacC3, and aacC4 genes. PCR primer to detect the aerolysin gene was designed using the conserved region of the genes for aerolysin and hemolysin of Aeromonas spp. This primer set successfully amplified the expected 414 bp PCR product with the DNA samples from the lake water. The aerolysin gene was detected in 7 of 12 DNA samples. When Southern hybridization of the gel with probe was performed, the aerolysin gene was detected in 10 of 12 DNA samples. However, the seasonal fluctuation of these genes was not found.

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Distributional Pattern of tetQ and aacC2 genes in Stream Water (하천에서 tetQ와 aacC2 유전자의 분포 양상)

  • 정재성;이영종;김종홍
    • The Korean Journal of Ecology
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    • v.22 no.5
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    • pp.305-309
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    • 1999
  • The occurrence of tetQ and aacC2 genes encoding tetracycline and gentamicin resistance determinant, respectively, was assessed in total bacterial community DNA isolated from Dongchon stream of Sunchon area. To examine the resistance potential of bacteria that were not cultured, total DNA from 1 liter of stream water was extracted by freeze-thaw method. The PCR technique was employed to determine the abundance of the target genes. The highest frequency of tetQ gene was obtained from site 1, located near the animal farms area, whereas the incidence of aacC2 was highest in site 5, the downstream area. These results showed that the occurrence of antibiotic resistance gene may be used as a convenient marker of water quality related to source.

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MPEG-2 AAC Encoder Implementation Using a floating-Point DSP (부동 소수점 DSP를 이용한 MPEG-2 AAC 부호차기 구현)

  • Kim Seung-Woo
    • Journal of Korea Multimedia Society
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    • v.8 no.7
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    • pp.882-888
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    • 2005
  • MPEG-2 Advanced Audio Coding (AAC) has already been standardized as a sophisticated next generation technology AAC provides an audio signal that has CD quality at 96-128kbps/stereo. This paper describes a high-quality and efficient software implementation of an MPEG-2 AAC LC Profile encoder. Common scalefactor and noisless coding are accelerated by $45\%$ and $27\%$, respectively, through the use of TMS320C30 instructions. The implemented encoder uses 7.5kWords of program memory, 18kWords of data ROM and 92kBytes of data RAM, respectively. The results of subjective Qualify test showed that the sound quality achieved at 96kbps/stereo was equivalent to that of MP3 at 128kbps/stereo.

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Occurrence of Tn3 Sequence Upstream of aacC2 Gene in Gentamicin Resistance R Plasmids (Gentamicin 저항성 R 플라스미드에 존재하는 aacC2 유전자의 상류부위에서 Tn3의 출현)

  • 한효심;김남덕;이영종;이효연;정재성
    • Korean Journal of Microbiology
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    • v.33 no.3
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    • pp.165-169
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    • 1997
  • Two gentamicin resistance R plasmids, pGM5 and pGM6, containing aacC2 gene were selected from environmental isolates. The gentamicin resistance determinants of R plasmids were cloned into the BamHI site of pUC18. Restriction enzyme map of inserted region of recombinant plasmids, pSYS and pSY6, and PCR results indicated that Tn3 sequence was located upstream of gentamicin resistance gene. Based on the restriction maps and susceptibility tests, it was concluded that the sequence of bla and 3' inverted repeat of Tn3 play a important roles in the expression of gentamicin resistance gene.

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Strength Characteristics according to the mixed CaO/$SiO_2$ Ratio to Autoclaved Aerated Concrete(AAC) used on the Exterior Panel in Buildings (건물 외벽 패널용 경량기포콘크리트(AAC)의 CaO/$SiO_2$ 혼합비에 따른 강도 특성 평가)

  • Kim, Young-Ho
    • Journal of The Korean Digital Architecture Interior Association
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    • v.11 no.3
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    • pp.35-42
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    • 2011
  • The exterior system of buildings, which is the typical curtain wall, has been made with glass and metal. Theses materials, however, have weaknesses such as inadequate insulating quality, short durability, combustibility and toxic substance. On the other hand, Autoclaved Aerated Concrete(AAC) or Autoclaved Lightweight Concrete(ALC) possess the great energy efficiency and the superb insulating quality as substitute of existing exterior system materials. In this research, strength characteristics and bubble dispersion of hydrothermal synthesis process of AAC based on CaO/$SiO_2$(C/S) ratio are analyzed. C/S ratio is determinated and bubble distribution and compressive strength are studied through the test of varied water-to-solid mineral ratio(W/S). In hydrothermal synthesis program, final C/S ratio is determined as 0.7 consider of the manufacturing process and hydrothermal synthesis is done at $180^{\circ}C$ for 7 hours. The analysis shows slurry has about 2,300cP viscosity and 0.56 specific gravity therefore it is expected AAC has the appropriate facility in the manufacturing process and Hydrates of AAC's Expansion.

Implementation of a 16-Bit Fixed-Point MPEG-2/4 AAC Decoder for Mobile Audio Applications

  • Kim, Byoung-Eul;Hwang, Sun-Young
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.33 no.3C
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    • pp.240-246
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    • 2008
  • An MPEG-2/4 AAC decoder on 16-bit fixed-point processor is presented in this paper. To meet audio quality criteria, despite small word length, special design methods for 16-bit foxed-point AAC decoder were devised. This paper presents particular algorithms for 16-bit AAC decoding. We have implemented an efficient AAC decoder using the proposed algorithms. Audio contents can be replayed in the decoder without quality degradation.

Bioavailability of Aspartic Acid Chelated Calcium in Calcium Deficient Rats (아스파르트산 킬레이트 칼슘의 칼슘 결핍쥐에서의 생물학적 유용성)

  • Park, Myoung-Gyu;Ha, Tae-Yul;Shin, Kwang-Soon
    • Journal of Nutrition and Health
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    • v.44 no.6
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    • pp.474-480
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    • 2011
  • Calcium (Ca) is an essential element to maintain body homeostasis. However, many factors disturb calcium absorption. Aspartic acid chelated calcium (AAC) was synthesized by new methods using calcium carbonate and aspartic acid. This study was carried out to investigate the bioavailability of AAC in Ca-deficient rats. The experimental groups were as follows: NC; normal diet control group, CD-C; untreated control group of Ca-deficient (CD) rats, CD-$CaCO_3$; $CaCO_3$ treated group of CD rats, CD-AAC; AAC treated group of CD rats, and CD-SWC; and seaweed-derived Ca treated group of CD rats. The Ca content of various types of Ca was held constant at 32 mg/day, and the four CD groups were fed for 7 days after randomized grouping. Ca content in serum, urine, and feces within feeding periods were analyzed to confirm Ca absorption. Serum Ca content was significantly higher in the CD-AAC (11.24 mg/dL) and CD-SWC (10.12 mg/dL) groups than that in the CD-C (8.6 mg/dL) group 2 hours following the first administration. The Ca content in feces was significantly lower in the CD-AAC (35.4 mg/3 days) and CD-SWC (71.1 mg/3 day) groups than that in the CD-$CaCO_3$ (98.7 mg/3 days) group (p > 0.05). AAC had a 2.3-fold higher absorption rate of Ca than that of SWC. No differences in fibula length were observed in the NC and CD groups. The fibula weights of the CD-AAC (0.33 g) and CD-SWC (0.33 g) groups increased compared to those in the CD-C (0.27 g) group; however, no significant difference was observed between the CD groups. We conclude that bioavailability of AAC is higher than that of seaweed-derived Ca or inorganic Ca. Thus, these findings suggest the AAC has potential as a functional food material related to Ca metabolism.

A Common Synthesis Filter for MPEG-2 BC/AAC Audio Using Recursive Structure (Recursive 구조를 이용한 MPEG-2 BC/AAC 오디오 공용 합성 필터)

  • 강명수;박세기;오신범;이채욱
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.6C
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    • pp.874-882
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    • 2004
  • MPEG Audio compression algorithm is the international standard for the digital compression of high quality audio using mechanism of the perceptual coding based on psychoacoustic masking. It is necessary to discuss the constraints on designing of common filter banks for MPEG-2 BC and MPEG-2 AAC decoder system, which is not Down yet, mapping audio signals from the time domain into the frequency domain. In this paper, we present an architecture of common synthesis filter whcih can be used for MPEG-2 BC and MPEG-2 AAC decoder using recursive structure. The proposed algorithm is based on recursive architecture that effectively performs common compulsion.

Architecture Design for MPEG-2 AAC Filter bank Decoder using Recursive Structure (Recursive 구조를 이용한 MPEG-2 AAC 복호화기의 필터뱅크 구현)

  • 박세기;강명수;오신범;이채욱
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.6C
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    • pp.865-873
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    • 2004
  • MPEG-2 Advanced Audio Coding(AAC) is widely used in the multi-channel audio compression standards. And it combines hi인-resolution filter bank prediction techniques, and Huffman coding algorithm to achieve the broadcast-quality audio level at very low data rates. The forward and inverse modified discrete transforms which are operated in the encoder and the decoder of the filter bank need many computations. In this paper, we propose suitable recursive structure at IMDCT processing for MPEG-2 AAC real-time decoder. We confirm the memory, the computation speed and complexity of the proposed structure.

A Study on the Synthesis of ACF/PP-g-AAc Hybrid Fibrous Adsorbent by Irradiation and Their Separation Properties of Heavy Metals (방사선 중합법에 의한 ACF/PP-g-AAc 혼성섬유 흡착제의 합성 및 중금속 분리 특성에 관한 연구)

  • 황택성;황대성;박정기
    • Polymer(Korea)
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    • v.24 no.1
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    • pp.1-7
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    • 2000
  • The ACF/PP-g-AAc copolymers were synthesized by the irradiational grafting of acrylic acid onto ACF/PP fabric. The synthesis of copolymer was evidenced by the bands at 1720, 3600~3100 $cm^{-1}$ / on FT-IR spectrum. After the adsorption of metal ions on ACF/PP-g-AAc copolymers, the morphology with the small deposits on the fiber surface were observed by SEM. The optimal time for the metal ion adsorption equilibrium on ACF/PP-g-AAc copolymers was 24 hrs and their adsorption capacities increased in the order of Mn$^{2+}$>Cu$^{2+}$>Co$^{2+}$>Ni$^{2+}$. The adsorption capacities of ACF/PP-g-AAc copolymers were invariable after more than 10 times of regeneration.eration.

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