• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.448-457
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• 1993

Deterioration of fish muscle is known to occur more quickly in the dark fleshed fish than in the white fleshed fish, causing by their high intestinal proteolytic activity. Muscle degradation which suffer post-mortem autoproteolysis is affected by trypsin with its unique activation function towards other enzymes. To compare physicochemical and enzymatic properties for the trypsins of the dark fleshed fish, trypsins from the viscera of anchovy (Engraulis japonica), and the pyloric caeca of mackerel (Scomber japonicus), yellowfin tuna (Thunnus albacores) and albacore (Thunnus alalunga) were purified through ammonium sulfate fractionation, benzamidine-Sepharose 6B, DEAE-Sephadex A-50, and Sephadex G-75 chromatography Two trypsins from mackerel (designated mackerel trypsin A and mackerel trypsin B), and one each from anchovy, yellowfin tuna and albacore were isolated as electrophoretical homogeneity, The purities of anchovy trypsin, mackerel trypsin A and B, yellowfin tuna trypsin, and albacore trypsin increased to 78.1, 4.8, 9.3, 120, and 160-fold, respectively, compared to crude enzyme solutions. Molecular weights of the trypsins from the dark fleshed fish estimated by SDS-polyacrylamide electrophoresis were ranged from 22kDa to 26kDa. The trypsins contained higher amount of glycine, serine and aspartic acid, and less amount of tryptophan, methionine, lysine and tyrosine. Optimal conditions for amidotici reactions of the enzymes were pH 8.0 and 45$^{\circ}C$ for anchovy trypsin, pH 8.0 and 5$0^{\circ}C$ for mackerel trypsin A and B, pH 9.0 and 55$^{\circ}C$ for yellowfin tuna trypsin, and pH 9.0 and 5$0^{\circ}C$ for albacore trypsin. It was supposed that the habitat temperature of the dark fleshed fish is slightly connected with the optimal reaction temperature of the trypsins of the fish.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.1
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• pp.64-70
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• 1996

Kinetic properties of typsins purified from dark-fleshed fish (anchovy, mackerel, yellowfin tuna, and albacore) were examined and analyzed on $benzoyl-_{D,L}-arginine-p-nitroanilide\;(BAPNA)$. The values of Km' and $k_{cat}$ of the purified trypsins from the four dark-fleshed fish were found to be $49.3{\mu}M$ and $90.9\;min^{-1}$ for anchovy, $53.7{\mu}M$ and $61.2min-^{-1}$ for mackerel A, $96.5{\mu}M$ and $76.6min^{-1}$ for mackerel B, $62.8{\mu}M$ and $46.6min^{-1}$ for yellowfin tuna, and $98.3{\mu}M$ and $47.7min^{-1}$ for albacore, respectively. The values of $K_i$ on $tosyl-_L-lysine$ chloromethyl ketone (TLCK) were determined to be $20.90{\mu}M$ for anchovy trypsin, $2.86{\mu}M$ for mackerel trypsin A, $3.90{\mu}M$ for mackerel trypsin B, $0.96{\mu}M$ for yellowfin tuna trypsin, and $1.82{\mu}M$ for albacore trypsin. Thus yellowfin tuna trypsin was the most sensitive to TLCK among all trypsins. The activities and catalytic efficiency of the trypsins purified from the temperate zone fish, anchovy and mackerel, were higher than those of the trypsins purified from yellowfin tuna and albacore which migrate widely from the tropic zone to the temperate zone.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.22 no.4
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• pp.21-31
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• 1986

The author investigated the relation between the catches of tuna species and the distribution of horizontal mean temperature at the Jepth of 10m and of vertical temperture sections in the different fishing grounds, using the date of catches in 1980, showing a relative good ones during six years from 1975 to 1980, and of oceanographic observations. Yellowfin and bigeye are mainly caught in South Equatorial Current regions including equatorial upwelling region in 5$^{\circ}$N to 5$^{\circ}$S, and albacore is mainly caught in Subtropical region in 20$^{\circ}$5 to 40$^{\circ}$5. The good fishing grounds of yellowfin and bigeye are made in the depth layer of 100 m to 250 m and temperature of 15$^{\circ}$C to 26$^{\circ}$C having a smooth gradient of thermocline in the Central Pacific between 180$^{\circ}$ and 1500W. But albacore is caught well in which the temperature of thermocline ranges from 100e to 25$^{\circ}$C and its gradient very smoothly. Approaching to the American Continent, the catches of yellowfin and big eye decrease because the thermocline becomes shallower and steeper at Eastern Pacific Region between 1500 and 800W.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.50 no.4
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• pp.556-566
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• 2014

Korean distant water tuna longline fishery commenced in 1966 in the Atlantic Ocean. Since then, it has become one of the most important fisheries of Korea. By early of 1970s, total amount of tuna caught in the Atlantic Ocean was the highest among other Korean distant water tuna fisheries, but has become minor since 1990s. The annual catch of tuna and tuna-like species by Korean tuna longline fishery in the Atlantic Ocean was about 1,900 mt in 2013. Bigeye tuna was the predominant species in species composition followed by yellowfin tuna, Albacore tuna, Blue shark and Swordfish. Korean distant water tuna longline fishery have mainly operated in the tropical area of the Atlantic Ocean ($20^{\circ}N{\sim}20^{\circ}S$, $20^{\circ}E{\sim}60^{\circ}W$), fishing ground was almost similar as in the previous years. The length frequency of major species (Bigeye tuna, Yellowfin tuna, Albacore tuna, Blue shark and Swordfish) were estimated. As the result of length (size) frequency data on main species caught by lonline fishery in the Atlantic Ocean, main length intervals of bigeye tuna caught in 2011, 2012 and 2013 were 120~125 cm, 160~165 cm and 130~135 cm, respectively. For yellowfin tuna, those were 125~130 cm, 150~155 cm, 145~150 cm and for albacore, 109 cm, 102 cm, 109 cm and 106 cm respectively. For swordfish caught in 2011, 2012 and 2013, main length intervals were 130~135 cm, 125~135 cm and 125~130 cm, respectively, and for blue shark, 195~200 cm in 2011 and 185~190 cm in 2012, 2013.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.4
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• pp.458-464
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• 1993

In the present paper enzymatic properties of the trypsins from the four dark fleshed fish were compared with each other and thermal stabilities of the enzymes were also investigated. The trypsins from the dark fleshed fish showed their activity only in BA-p-NA substrate of the amide substrates such as BA-p-NA and SP-p-NA, and BAEE and TAME of the ester substrate such as ATEE, BAEE, BTEE, and TAME. The enzymes were strongly inhibited by the serine protease inhibitors such as antipain, leupeptin, TLCK, DFP and SBTI, and were also inhibited by such metal ions as Cu$^{2＋}$ and Hg$^{2＋}$, but fairly activated by $Mg^{2＋}$. Denaturation constants of the enzymes were 13.4$\times$10$^{-4}$ sec$^{-1}$ for anchovy trypsin, 47.18$\times$10$^{-4}$ sec$^{-1}$ for mackerel trypsin A, 34.06$\times$10$^{-4}$ sec$^{-1}$ mackerel trypsin B, 42.28$\times$10$^{-4}$ sec$^{-1}$ for yellowfin tuna trypsin and 16.6$\times$10$^{-4}$ sec$^{-1}$ for albacore trypsin at 55$^{\circ}C$. The activation energies of the trypsins at a temperature range of 3$0^{\circ}C$ to 5$0^{\circ}C$ were estimated to be 13.91 ㎉/mole for anchovy trypsin, 11.61㎉/mo1e and 8.43㎉/mole for mackerel trypsin A and for mackerel typsin B, 4.35㎉/mole for yellowfin tuna trypsin, and 3.76㎉/mole for albacore trypsin.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.32 no.1
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• pp.24-32
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• 1996

This paper described on relation between the catches of tuna and the distribution of water temperature of eastern fishing ground of Tropical region in the Pacific Ocean. The data of catches and water temperature used in this paper were based log book which # 27 CHENG RONG(Gross tonnage : 399 ton) had been worked eastern fishing ground(Lat : 09$^{\circ}$N- 14$^{\circ}$S, Long : 115$^{\circ}$- 149$^{\circ}$W)from January to October, 1991. The obtained result are as follows : 1. On the relation between the catches and the geographical distribution, bigeye tuna was higher catches at Lat 4$^{\circ}$- 9$^{\circ}$N, Long 135$^{\circ}$- 139$^{\circ}$W area in the equatorial counter current region where surface water temperature was range of 27.5$^{\circ}C$ to 27.9$^{\circ}C$, yellowfin tuna was higher catches at Lat 4$^{\circ}$- 9$^{\circ}$S, Long 145$^{\circ}$- 149$^{\circ}$W in the south equatorial current region where surface water temperature was range of 28.$0^{\circ}C$ to 28.4$^{\circ}C$ and albacore tuna was higher catches at Lat 10$^{\circ}$- 14$^{\circ}$S, Long 120$^{\circ}$- 124$^{\circ}$W area in the south equatorial current region where surface temperature was range of 26.5$^{\circ}C$ to 26.9$^{\circ}C$ 2. On the relation between catches and distribution of vertical water temperature, bigeye tuna was higher catches at the water temperature of 1$0^{\circ}C$ to 12$^{\circ}C$ on depth layer between 300m and 360m, yellowfin tuna was higher catches at the water temperature of 15$^{\circ}C$ to 19$^{\circ}C$ on depth layer between 180m and 280m and albacore tuna was higher catches at the water temperature of 12$^{\circ}C$ to 14$^{\circ}C$ on depth layer between 280m and 310m. Above the result, it seemed that bigeye tuna distributed deeper layer than yellowfin and albacore tuna.

• Journal of environmental and Sanitary engineering
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• v.6 no.1
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• pp.95-102
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• 1991

This study was carried out to determine the contamination level of heavy mentals in canned fishes on the market. Contents of tin, lead, iron and zinc were analyzed by Atomic Absorption Spectrophtometer. Contents of total mercury were determined by mercury analyzer. The results were as follows. 1. The highest value of tin was $1.7706{\pm}0.3216$pprn in canned yellow pin & skip jack(mix) tuna, the lowest was 1.2109 ${\pm}$ 0.0769ppm in canned mackerel pike. 2. The highest value of lead was 0.1657 ${\pm}$ 0.0530 pprn in canned yellow pin tuna, the lowest was 0.0335k 0.0100 pprn in canned mackerel pike. 3. The highest value of iron and zinc was 22.5943 ${\pm}$ 0.7945 ppm and 13.4556 ${\pm}$ 0.6774 pprn in canned sardine fish, the lowest was 4.3514 ${\pm}$ 0.3607 pprn and 3.8773 ${\pm}$ 0.1914 pprn in canned albacore tuna. 4. The total mercury contents was detected 0.0531~0.3386 pprn in three kinds of canned tuna and could not be detected with the others.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.51 no.3
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• pp.286-297
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• 2018

We analyzed scientific observer data to identify species composition and catch trends of Korean tuna longline fisheries in the Indian Ocean. The data were collected by Korean scientific observers onboard Korean tuna longline fishing vessels in the Indian Ocean from 2010 to 2015. The observers identified 94 species including 5 tuna, 6 billfish, 11 shark, 7 other fish, 5 seabird and 1 sea turtle. Albacore tuna Thunnus alalunga and southern bluefin tuna Thunnus maccoyii were the dominant tuna species, and swordfish Xiphias gladius were the most commonly captured billfish. During the survey periods, 87 seabirds were incidentally bycaught. Black-browed albatross Diomedea melanophris was the dominant seabird species in bycatch, especially in 2010. Cluster analysis showed difference in the species composition of catches between the eastern and western Indian Ocean.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.4
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• pp.449-461
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• 2023

The aim of this study was to conduct an ecosystem-based fishery risk assessment of tuna fisheries in the Western Indian Ocean. We selected gillnet, purse seine, hand line, baitboat, and longline fisheries as the target fisheries method, and selected longtail tuna (Thunnus tonggol), narrow-barred Spanish mackerel (Scomberomorus commerson), kawakawa (Euthynnus affinis), skipjack tuna (Katsuwonus pelamis), yellowfin tuna (T. albacares), bigeye tuna (T. obesus), albacore tuna (T. alalunga) and swordfish (Xiphias gladius) as the target species. The risk score for the size at the first capture in sustainability objective was high, especially, for the purse seine and baitboat fisheries using the fish aggregating devices (FADs). The risk score for the bycatch in the biodiversity objective was high for the gillnet fishery, and the gillnet fisheries using FADs showed high risks for the habitat quality objective due to the loss of the fishing gears. With regards to the socio-economic benefits objective, the risk score of the sales profits was low due to high sales of the tuna fisheries. The ecosystem risk score in the Western Indian Ocean was estimated to be moderate, although management is required for some of the indicators that have high-risk scores.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.45 no.1
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• pp.22-33
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• 2009

Ecological risk assessment(ERA), developed in Australia, can be used to estimate the risk of target, bycatch and protected species from the effects of fishing using limited data for stock assessment. In this study, we employed the ERA approach to estimate risks to tunas, billfishes, sharks, sea turtles and other species by the Korean tuna longline fishery in the Western and Central Pacific Ocean using productivity and susceptibility analyses of the ERA based on low(<1.30), medium(1.30-1.84) and high risk(>1.84). Albacore, bigeye, yellowfin, skipjack and bluefin tunas were generally evaluated in the medium risk. The susceptibility of tuna species, however, had higher risks than the productivity. Billfishes were also at medium risk, while sharks were at high risk by the tuna longline fishery. The risk of productivity was generally high, because most sharks caught by the tuna longline fishery have high longevities, i.e., over 10 years, including ovoviviparous species. Susceptibility, which is related with the selection of fishing gear, was also high, because the longline fishery has no gear modifications to prevent bycatch of protected species. Not only target tuna species were influenced by the tuna longline fishery in the Western and Central Pacific Ocean, but also nontarget species, such as pomfret, mackerels rays, sea turtle were done. Ecosystem-based fishery assessment tools, such as productivity and susceptibility analysis(PSA), have the ability to provide broad scientific advice to the policy makers and stakeholders.