• Title/Summary/Keyword: aldosterone

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Changes of Aldosterone Secretion Rate Following Furosemide Administration in Normotensive Subjects with High Sodium Intake (Furosemide 투여후 Aldosterone 분비율의 변동)

  • Sung, Ho-Kyung;Ryu, Yong-Wun;Koh, Joo-Hwan
    • The Korean Journal of Nuclear Medicine
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    • v.10 no.1
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    • pp.55-60
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    • 1976
  • Marked augmentation of urinary aldosterone excretion following furosemide administration was observed in previous experiment. In this study, author measured the changes of aldosterone secretion after furosemide administration in normotensive young volunteers with high sodium intake. After intravenous injection of $1.2-^3H-aldosterone$, urine samples were collected in course of time until 24 hours after the injection. Furosemide administration was done at 30 minutes prior to aldosterone injection. Specific activities of $^3H-aldosterone$ during and after diuresis were measured and aldosterone secretion rates were calculated dividing the doses by specific activities. Results were as followed 1. Furosemide resulted in a marked increase in urinary aldosterone excretion. 2. Furosemide lead to an increase in both sodium and potassium excretion. 3. Aldosterone secretion rate was also increased during furosemide diuresis, but the rate was smaller than that of urinary excretion. 4. Continuous modest increase in aldosterone secretion rate was shown after diuresis and total excess amount of aldosterone secretion for 24 hrs was equivalent to the amount of aldosterone excretion produced by diruesis. 5. Abrupt marked loss of circulating aldosterone produced by diuresis was supplemented by long lasting increase in secretion for over twenty four hours.

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Effects of Sodium Restriction and Potassium Supplement on Aldosterone Secretion Rate In the Normal Korean (한국인의 Aldosterone 분비율에 미치는 Na 섭취제한 및 K 투여의 영향)

  • Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.10 no.2
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    • pp.23-28
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    • 1976
  • Author have already reported that urinary aldosterone excretion of the Korean who usually eat high sodium diet is significantly lower comparing with the American, although the plasma aldosterone concentration is identical in the former with that of the latter. Measurement of urinary aldosterone excretion and Plasma concentration only is insufficient to establish the pressence and/or mode of evolution of the Korean. In this experiments, aldosterone secretion rate(ASR) was measured in normotensive Korean during high and low dietary sodium intake with or without additional potassium supply. Results were as follows; 1) In normal Korean, dietary sodium restriction resulted in appreciable increase in ASR, and a sustained increase in urinary aldosterone excretion with an increase in plasma level. 2) Oral potassium loading easily stimulated the adrenal cortex of the Korean who already adapted to a high sodium diet when dietary sodium is still identical with not·mal American. 3) Quantitative relationships between aldosterone secretion rate, plasma concentration and urinary excretion of aldosterone were altered by potassium loading. 4) Urinary aldosterone excretion didn't reflect concurrent increase aldosterone secretion in subjects with Potassium intake. It was discussed that the changes of tile relationships and of adrenal hyper response on Potassium Beading in the Korean will be elucidated by measuring the metabolic clearance rate.

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A Relation of Urinary Aldosterone Concentration to K/Na Ratio Following Furosemide Administration in Normal Subjects with High Sodium or Low Sodium Intake (Furosemide 투여후의 뇨중 Aldosterone 농도대 K/Na 비사이의 관계)

  • Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.9 no.2
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    • pp.33-39
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    • 1975
  • Changes of urinary aldosterone excretion, concurrent sodium and potassium excretion following furosemide administration were studied in normotensive young Korean with high sodium intake, moderate sodium restriction and marked sodium depletion. After intravenous injection of furosemd 40mg, plasma and urine samples were collected at every thirty minutes for two hours. Plasma-and urinary aldosterone, electrolyte concentration and urine flow rate were measured by means of radioimmunoassay or flamephotometry. Relations of urinary aldosterone to concurrent sodium or potassium/sodium ratio, and of urinary aldosterone to concurrent plasma aldosterone activity were studied. Following were the results: 1. Furosemide administration resulted in a increased urinary aldosterone concentration and unchanged or somewhat decreased sodium concentration in course of time after the injection. 2. Urinary potassium concentration showed initial decrease and subsequent increase in course of time after furosemide administration and it resulted in a gradual increase in urinary potassium/sodium ratio. 3. Studying the relations between urinary aldosterone excretion and potassium/sodium excretion ratio, or sodium excretion were meaningless because of the urinary flow rate after the injection was decreased with time course. 4. Furosemide administration showed a good relationship of urinary aldosterone concentration to concurrent potassium/sodium ratio rather than concurrent sodium concentration in subjects with sodium restriction, but no meaningful relationship was detected in subjects with high sodium intake because increasing rate of the ratio was not so wide. 5. Furosemide also resulted a reasonable relation of plasma aldosterone concentration to concurrent urinary aldosterone concentration especially during low sodium intake. 6. Above results suggested that relation of urinary aldosterone concentration to K/Na ratio following furosemide administration during sodium restriction is significant and has a benefit to reduce the variation induced by kalemic change showing in the diragram for daily aldosterone to sodium excretion.

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Aldosterone Up-regulates Production of Plasminogen Activator Inhibitor-1 by Renal Mesangial Cells

  • Yuan, Jun;Jia, Ruhan;Bao, Yan
    • BMB Reports
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    • v.40 no.2
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    • pp.180-188
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    • 2007
  • In vivo studies have demonstrated that aldosterone is an independent contributor to glomerulosclerosis. In the present study, we have investigated whether aldosterone itself mediated glomerulosclerosis, as angiotensin II (Ang II) did, by inducing cultured renal mesangial cells to produce plasminogen activator inhibitor-1 (PAI-1), and whether these effects were mediated by aldosterone-induced increase in transforming growth factor $\beta_1$ (TGF-$\beta_1$) expression and cellular reactive oxygen species (ROS) activity. Quiescent rat mesangial cells were treated by aldosterone alone or by combination of aldosterone and spironolactone, Ang II, neutralizing antibody to TGF-$\beta_1$ or antioxidant Nacetylcysteme (NAC). This study indicate that aldosterone can increase PAI-1 mRNA and protein expression by cultured mesangial cells alone, which is independent of aldosterone-induced increases in TGF-$\beta_1$ expression and cellular ROS. The effects on PAI-1, TGF-$\beta_1$ and ROS generation were markedly attenuated by spironolactone, a mineralocorticoid receptor antagonist, which demonstrate that mineralocorticoid receptor (MR) may play a role in mediating these effects of aldosterone.

Proteome-based Identification of Proteins Regulated by Aldosterone in Rat Kidney Cortex (프로테오믹스 기술을 통한 쥐의 신장 피질에서 알도스테론(Aldosterone)에 의해 조절되는 단백질 동정)

  • Song, Mi-Na;Jeon, Hong-Bae;Choi, Hyo-Jung;Kwon, Tae-Hwan;Baek, Moon-Chang
    • YAKHAK HOEJI
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    • v.54 no.3
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    • pp.180-186
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    • 2010
  • Aldosterone, mineralocorticoid hormone, has important functions related to the regulation of blood pressure and balance of fluids and electrolytes in the distal region of the nephron. By genomic and non-genomic action of aldosterone, the physiological kidney functions are modulated. However, many of them except several kind of sodium channel have not been identified and analyzed yet. In this study, proteomic technologies with two-dimensional gel electrophoresis (2-DE) gel using aldosterone rat model were applied to analyze and identify the aldosterone dependently expressed proteins in rat kidney cortex. As a result, the established aldosterone rat model exhibited the normal physiological responses to aldosterone and modulated proteins were identified, which included 15 increased and 3 decreased proteins on 2-DE analysis. Among them, 11 proteins were identified as changed proteins by LC-MS/MS analysis. These proteins identified as aldosterone induced proteins were involved in several cellular pathways such as cytoskeleton remodeling, energy metabolism, amino acid metabolism, and chaperone process. In conclusion, our data could provide the insights into the new mechanism underlying regulation of kidney functions by aldosterone.

Effects of Na Restriction, K Supplement and Diuresis on Aldosterone Metabolic Clearance in the Normal Korean (한국인의 Aldosterone 대사 제거율에 미치는 Na 섭취제한, K 보충투여 및 이뇨의 영향)

  • Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.11 no.1
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    • pp.37-44
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    • 1977
  • In the previous study of the release, excretion, and plasma concentration of aldosterone in normal Koreans, the author found that urinary aldosterone excretion and aldosterone secretion rate of the Korean who usually take high amount of salt are significantly lower, in compared to Americans, although the plasma concentration is only tended to be low. The control of plasma aldosterone level depends on the secretion rate and the metabolic clearance of the hormone. In this experiments, the metabolic clearance rate of aldosterone was determined in normotensive korean and the effects of adrenal stimulations on the rates were also studied in the same subjects. The metabolic clearance rate of the normal Korean was not significantly different from those of the American, and shown a little increase in response to sodium restriction. These results indicate that the decrease in secretion rate rather than the increase in metabolic clearance Tate is the major factor maintaining lower plasma aldosterone level. After furosemide diuresis, on the contrary, the removal of aldosterone showed significant the decrease despite slight increase of secretion rate. This suggest that the reduction in metabolic clearance rate of the hormone during volume depletion found to be major cause of high plasma concentration. Additional potassium supply produced detectable decrease of metabolic clearance rate, but the changes were smaller than that of secretion rate, which suggested that the higher secretion rate could account for elevated plasma concentration of aldosterone rather than metabolic clearance. Above results also support author's previous evidences that the normal Korean who already adapted to a high sodium diet have ability to produce adequate aldosterone activity without producing detectable changes on the metabolic clearance rate under the condition of sodium restriction with approp riate potassium intake.

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Effects of Aspirin and Furosemide on Plasma Aldosterone Level in Rabbits (토끼의 혈장내 Aldosterone 농도에 미치는 Aspirin과 Furosemide의 영향)

  • Suh, Y.J.;Lee, K.H.;Kim, O.N.;Lee, S.B.;Cho, K.C.
    • The Korean Journal of Pharmacology
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    • v.20 no.2
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    • pp.1-6
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    • 1984
  • It has been generally recognized that the secretion of aldosterone is mainly regulated by angiotensin II in animals and humans, however, potassium and ACTH are also proposed as other humoral factors involved in the aldosterone secretory process. Recently, stress, anesthesia, adrenergic stimulation, low sodium intake or water deprivation stimulate plasma renin activity, while high sodium intake and deoxycorticosteroid have been reported to cause suppression of renin activity in animals. It seems that overall response of aldosterone secretory mechanisms reflects complex interactions both intrarenal and extrarenal components. Furosemide has been widely used to investigate the control of renin secretion by the kidney, and the relationship between diuretics and the disposition of endogenous aldosterone were reported (Oh, 1984). The sequential with 10 min interval samples of plasma were collected following administration of furosemide(1 mg/kg), aspirin(10 mg/kg), respectively. And also similar experiment was performed in the propranolol (10 mg/kg) pretreated rabbits. The results were as follows : 1) The concentration of plasma aldosterone was average of $426.I{\sim}485.5pg/ml$ in normal rabbits. Plasma concentrations of aldosterone rised significantly after injection of furosemide during 50 min, and the rise of plasma aldosterone was blocked by the propranolol pretreatment 2) Significant fall in the plasma level of aldosterone after injection of aspirin was noted. This result indicates that the increased secretion of aldosterone induced by furosemide administration is mediated through ${\beta}-receptors$, and the possible role of prostaglandin is substantiated.

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Primary aldosteronism with an aldosterone-producing adenoma and contralateral adrenal hyperplasia: A case report (양측성 부신비대와 알도스테론 생성 선종이 동시에 발현된 증례)

  • Park, Hye Won;Lee, Sang Ah
    • Journal of Medicine and Life Science
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    • v.17 no.2
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    • pp.60-63
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    • 2020
  • Primary aldosteronism has been found more often among patients with hypertension. Primary aldosteronism can be caused by an aldosterone-producing adenoma, bilateral adrenal hyperplasia, or rarely by an adrenal carcinoma. An initial diagnostic test for aldosteronism is a measurement of the plasma renin activity and aldosterone concentration. For example, up to 20% of patients with hypertension showed increased plasma aldosterone concentration/renin activity ratio. If surgery is planned, an adrenal vein sampling is necessary for exact localization. Spironolactone, an aldosterone antagonist, is the drug of choice for patients with an aldosterone-producing adenoma or hyperplasia. It can control elevated blood pressure in most primary aldosteronism patients. However, unilateral laparoscopic adrenalectomy is the best treatment for aldosterone-producing adenoma or asymmetrical aldosterone production in patients with uncontrolled hypertension. Here we report a patient with primary aldosteronism caused by unilateral adrenal hyperplasia and a contralateral adrenal adenoma who required as many as five different kinds of antihypertensive medications for controlling elevated blood pressure. The adrenal adenoma was successfully removed by unilateral adrenalectomy and the blood pressure had been controlled well after the surgery.

Alteration of Hemorrhagic Aldosterone Response During Sodium Restriction, Potassium Supplement and Diuresis (Na 섭취제한, K 투여 및 이뇨 기간중 실혈성 Aldosterone 반응의 변동)

  • Sung, Ho-Kyung;Ryu, Yong-Wun;Koh, Joo-Whan;Park, Kee-Won;Lee, Jang-Kyu
    • The Korean Journal of Nuclear Medicine
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    • v.11 no.1
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    • pp.3-8
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    • 1977
  • Effect of sodium restriction with or without potassium supplement and furosemide diuresis on plasma aldosterone response to mild hemorrhage were studied in normotensive young volunteers. After an overnight fast, blood were drawn just before and 10, 20, 30, 50, 70, 90, and 120 minutes after the $^3H$-aldosterone injection. The sum of blood delivered reached over 100ml(during two hours). Plasma aldosterone and renin were measured by means of radioimmunoassay. The results were as followed; 1. Hemorrhage resulted in a moderate increase in plasma aldosterone level of volunteers with normal diet. 2. The mean figures of plasma aldosterone in subjects with sodium restriction and diuresis were likewise significantly increased by hemorrhage, however, the figure of the subjects with potassium supplement who already shown higher plasma level was without effect on hemorrhage. 3. Hemorrhage produced slight decrease in serum sodium concentration in every experimental conditions, although the changes were not significant. 4. Plasma renin activities after the hemorrhage followed a similar pattern with that of aldosterone, increased during sodium restriction or diuresis and unaffected during potassium supplement.

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Negative noxiousness of aldosterone analogue-induced hypertension and inhibition of aldosterone by silver spike point electrical stimulation (Aldosterone 유도체-고혈압의 음성적 유해와 은침점전기자극의 aldosterone 억제)

  • Chon, Ki-Young;Kim, Jung-Hwan;Kim, Soon-Hee;Min, Kyung-Ok
    • Journal of Korean Physical Therapy Science
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    • v.10 no.2
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    • pp.199-207
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    • 2003
  • The present study examined that in vivo/vitro test is investigated in normotensive sham-operated rats(NSR) and aldosterone-analogue deoxycorticosterone acetate (DOCA)-salt hypertensive rats(ADHR) and that the antiliypertensive effect was induced by silver spike point(SSP) electrical stimulation at meridian points(CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52), specifically, such as aldosterone in 24 hour urine analysis from normal volunteer. The heart weight, the tickness of vascular wall, collagen fiber and the systolic blood pressure were significantly increased in ADHR than that in NSR. The required time of PSS-induced resting tone and the phosphorylation of stress-activated protein kinase/c-Jun N-terminal protein kinase(SAPK/JNK) were significantly increased in ADHR than that in NSR. However, the Kv currents were significantly decreased in ADHR than that in NSR. The current of 1 Hz continue type of SSP electrical stimulation significantly decreased in excretion of urine aldosterone from normal volunteer. These results suggest that the development of aldosterone analogue-induced hypertension is associated with changed heart weight, content of collagen fiber, tickness of vascular wall, blood pressure, resting tone, voltage-dependent K+ current(Kv) and phosphorylation of SAPK/JNK, which directly affects blood pressure. Therefore the hypertension is a risk factor on cerebrovascular disease. Moreover, These results suggest that the SSP electrical stimulation, especially current of 1 Hz continue type, significantly regulates excretion of urine aldosterone from volunteer.

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