• 동아시아식생활학회지
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• 제17권6호
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• pp.822-826
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• 2007

계란의 저장성 증진 효과를 검토하기 위해 계란 표면을 sodium alginate 용액으로 코팅하여 $5^{\circ}C,\;25^{\circ}C$에서 저장하면서 저장성 실험 및 코팅 처리된 계란 표면의 미생물 억제 효과 시험을 하였다. 살모넬라균과 같이 음식물의 섭취를 통해서만 감염되는 식중독의 원인 균의 증식 억제 효과 시험에서 $5^{\circ}C$에서 저장 했을 때 대조군에 비해 0.5% sodium alginate처리군이 뛰어난 세균 증식 억제 효과를 보였다. 계란의 pH는 저장 기간에 $CO_2$를 방출하거나 저장 온도가 증가함에 따라 증가하였다. 코팅된 계란의 pH가 대조군의 pH보다 낮게 나타났다. $5^{\circ}C$의 저장 온도에서 대조군의 pH가 7.72에서 7.94의 변화를 보였으며, 0.5%의 sodium algiante 용액으로 코팅한 계란에서는 7.72에서 7.85의 pH 변화가 나타났다. $5^{\circ}C$와 $25^{\circ}C$에 30일간 저장하면서 난황의 신선도를 나타내는 난황 계수와 계란의 품질을 평가하는 종합적인 수치인 Haugh unit를 측정하였다. Haugh unit은 30일간 저장하였을 경우 대조군인 경우 66.02, 0.5% sodium alginate로 코팅한 경우는 70.37로 얻어졌다. 난황 계수는 0.5% 농도의 시료로 코딩한 계란에서 대조군의 0.39보다 높은 0.42로 나타났다. 이런 연구 결과를 통해서 sodium alginate의 코팅 형성 능력이 계란의 표면에 유해 세균을 억제하고 저장성을 증진시킬 수 있다는 결과를 얻었다.

• Journal of Pharmaceutical Investigation
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• 제32권4호
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• pp.277-284
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• 2002

Lansoprazole, pharmaceutics for acid-related diseases, is unstable in low pH environments and generally coated with enteric polymer to obtain gastroresistance in stomach. Because its storage stability is influenced by acidic substitutes of enteric polymer, alkaline chemicals wεre generally addεd to dosage form as a stabilizer. In this experience, we coated lansoprazole bead with sodium alginate and evaluated the effect of bead size and sodium alginate coating on the storage stability and dissolution profile of lansoprazole. Sodium alginate solution containing lansoprazole was sprayed as a droplet into 3% (w/v) $CaCl_2$ solution and the resultant bead was coated with starch, sodium alginate, and hydroxypropyl methylcellulose phthalate. The content of lansoprazole granule not coated with sodium alginate decreased to 57.96% of initial content when stored at a severe condition for 4 weeks, but that of lansoprazole granule coated with sodium alginate before enteric coating decreased little and as the thickness of sodium alginate film increased, the content of bead didn't decreased for 4 weeks. Sodium alginate film also improved the gastroresistance without much influencing the maximum dissolution rate.

• 한국식품과학회지
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• 제38권6호
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• pp.762-766
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• 2006

골판지 상자의 수분저항성을 증진시키기 위해 골판지 상자 제조에 사용되는 라이너지의 표면을 알긴산으로 표면코팅을 하기 위한 최적 조건을 결정하기 위하여 농도, 가소제의 종류와 사용량, 알긴산의 불용화를 위한 다가 금속이온의 종류와 최적농도 및 침지시간 등을 결정하였다. 라이너지의 표면코팅을 위한 알긴산의 농도는 2.5%(w/w)가 적합하였으며, 가소제인 글리세롤은 알긴산 대비 35%의 농도로 사용하는 것이 적합하였고, 알긴산 필름의 불용화를 위해서는 2% $CaCl_2$ 용약을 사용하여 3분간 처리하는 것이 라이너지의 수분에 대한 저항성 증진에 최적조건을 결정되었다. 다가 금속이온으로 $CA^{2+}＄ 이외에 $Cu^{2+}＄를 사용했을 때 낮은 투습계수와 수분용해도를 나타냈다. 가소재의 종류에 따라서는 수분 흡습에 대한 저항성이 큰 솔비톨을 첨가 했을 때 라이너지의 수분저항성이 유의적인 차이를 보이며 증가하였다.

• Food Science and Biotechnology
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• 제15권6호
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• pp.825-832
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• 2006

The effect of alginate coating in combination with an anti-browning agent on increasing the post-cutting shelf life and improving the quality of minimally processed apple cubes was studied during storage at room temperature for 5 days. A simple coating technique involving the chemical cross-linkage of alginate by calcium was used. Statistical-based experimental designs were applied to improve the quality of the alginate-coated apple cubes (ACAC). Plackett-Burman design was first used to determine the main factors influencing the preservation of the original weight, color, and texture of ACAC. Among these variables, alginate concentration ($X_1$), dipping time ($X_2$), and dipping temperature ($X_3$) significantly influenced the ACAC weight and color (confidence levels above 90%). Subsequently, the effects of the 3 main factors were further investigated by a central composite design. The polynomial models developed by response surface methodology were adequate to describe the relationships between the studied factors and the responses. Overall optimization conducted by superimposing the curves of the responses enabled the determination of an optimal range of the independent variables in which the five responses were simultaneously optimized. The point chosen as representative of this optimal area corresponded to $X_1=2.98%$, $X_2=0.85\;min$, and $X_3=55^{\circ}C$ and under these conditions the model predicted weight loss=0.522%, relative hardness=1.517, ${\Delta}E=1.423$, browning inhibition=93.403%, and ${\Delta}L=0.158$.

• The Plant Pathology Journal
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• 제24권1호
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• pp.67-73
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• 2008

Seedling damping-off and bottom rot caused by Rhizoctonia solani are yield limiting diseases of crisphead lettuce. To provide biocontrol measure in the management of the diseases, biocontrol strain Pseudomonas aeruginosa LY-11 was isolated from lettuce rhizosphere and introduced into crisphead lettuce rhizosphere by the seed coating delivery method. Alginate was used as a coating material to generate beads containing $10^6-10^{6.5}$ colony-forming units (CFUs) of viable bacterial cells of LY-11. When seeds germinated from the alginate beads containing the strain LY-11, the bacteria established mostly in plant rhizosphere to maintain at least $10^4$ CFU per gram of plant tissues. Crisphead lettuce seedlings germinated from the entrapped seeds were less affected from damping-off and bottom rot with disease control values of 70.4% and 85.4% respectively. Although P. aeruginosa LY-11 colonized plant rhizosphere and not phyllosphere, the result indicated that bottom rot caused by the foliar inoculation of R. solani was effectively reduced by the rhizobacteria. All data suggested that immobilized rhizobacterial application in seeds by alginate coating could control damping-off and induce induced systemic resistance of crisphead lettuce to reduce bottom rot.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.570-573
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• 2005

분무법을 이용한 alginate microsphere를 제조한 결과 구형을 형성하는 것을 관찰하였고, 이러한 alginate microsphere에서 alginate 농도가 증가할수록 크기는 작아지고, chitosan/alginate microsphere에서 chitosan의 농도가 증가할수록 그 크기가 증가하는 것을 확인하였다. OVA의 방출정도는 alginate microsphere에서 alginate 농도가 증가할수록 잘 이루어지지 않았고, HCl buffer에서보다 PBS buffer에서 방출이 잘되는 것을 확인하였다. Chitosan/alginate microspheres에서는 chitosan의 농도가 증가할수록 방출이 잘되지 않았고, 이는 alginate microsphere에서와 마찬가지로 PBS buffer에서 방출이 잘 이루어지는 것을 확인하였다.

• 한국수산과학회지
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• 제49권1호
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• pp.20-25
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• 2016

This study elucidated the effects of physicochemical factors on the production of cooked rice analogs using calcium alginate gels. Cooked rice analogs were prepared using various sodium alginate concentrations, agitation speeds, dropping distances, coating times, curing times and heating times. The diameter ratio and rupture strength of authentic cooked rice were 0.38 and 268.4 kPa, respectively. The diameter ratio of the analogs prepared with 0.7% (w/v) sodium alginate was 0.39, which was the closest to that of authentic cooked rice. When sodium alginate solution (0.7%, w/v) was dropped into calcium chloride solution (2%, w/v) via a nozzle, the diameter ratio of the analogs at an agitation speed of 520 rpm was 0.39. The optimal dropping distance was 8 cm and the optimal coating and curing times were each 20 min. The analogs were coated with β-cyclodextrin to improve their physical properties. The diameter ratio of the coated analogs was little changed; however, the rupture strength decreased slightly after heating for 60 min at 95°C.

• 펄프종이기술
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• 제38권1호통권113호
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• pp.45-53
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• 2006

This study was carried out to produce high functional and biodegradable coating agents for pulp mold by evaluating various kinds of biodegradable polymers. Five kinds of biodegradable polymers were used. In addition, the mixture of the carboxymethylated starch and biodegradable polymers(${\kappa}$-carrageenan, chitosan) were used for mixed coating agents. Physical properties of coated paperboards were evaluated. Conclusions obtained were as follows. 4% ${\kappa}$-carrageenan and 5% chitosan showed higher water and oil resistance. 10% sodium alginate, 4% corn zein and 15% polycaprolactone showed high water resistance while no improvement was found on oil resistance. The optimum mixture ratios for the mixed coating agents were 90:10(carboxymethylated starch : ${\kappa}$-carrageenan) and 50:50(carboxymethylated starch : chitosan). Since these mixed coating agents have excellent biodegradability with higher water and oil resistance, these can be used for the environmental-friendly coating agents.

• Journal of Pharmaceutical Investigation
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• 제31권2호
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• pp.101-106
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• 2001

Alginate-chitosan (anion-cationic polymeric complex) was prepared to control the release rate of silver sulfadiazine (AgSD). Na-alginate (2%) solution containing AgSD was gelled in $CaCl_2$ solution. The gel beads formed were immediately encapsulated with chitosan (CS). The gel matrix and membrane were then reinforced with chondroitin-6-sulfate (Ch6S). Release rate of AgSD from the gel matrix was investigated by placing alginate beads in the sac of cellulose membrane simmered in HEPES-buffer solution. The concentration of AgSD released was analyzed by UV at 264 nm. Incorporation capacity of AgSD in Ca-alginate gel was more than 90%. Alginate-Ch6S-CS could control the release rate of AgSD. The amount of AgSD release was dependent on the AgSD loading dose. Incorporation of tripolyphosphate (polyanionic crosslinker) onto the alginate-Ch6S-CS bead increased the release rate of AgSD. Collagen-coating had no influence on the AgSD release rate. Alginate-Ch6S-CS beads with a sufficiently high AgSD encapsulation were capable of controlling the release of the drug over 10 days. In summary, alginate-Ch6S-CS beads could be used as a sustained delivery for AgSD and provide local targeting with low silver toxicity and patient discomfort.

• Archives of Pharmacal Research
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• 제27권1호
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• pp.118-126
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• 2004

The aqueous extract of European mistletoe (Viscum album, L.) has been used in cancer therapy. The purified mistletoe lectins, main components of mistletoe, have demonstrated cytotoxic and immune-system-stimulating activities. Korean mistletoe (Viscum album L. coloratum), a subspecies of European mistletoe, has also been reported to possess anticancer and immunological activities. A galactose- and N-acetyl-D-galactosamine-specific lectin (Viscum album L. coloratum agglutinin, VCA) with Mr 60 kDa was isolated from Korean mistletoe. Mistletoe preparations have been given subcutaneously due to the low stability of lectin in the gastrointestinal (GI) tract. In the present study, we investigated the possibility of alginate/chitosan microcapsules as a tool for oral delivery of mistletoe lectin. In addition, our strategy has been to develop a system composed of stabilizing cores (granules), which contain mistletoe lectin, extract or powder, coated by a biodegradable polymer wall. Our results indicated that successful incorporation of VCA into alginate/chitosan microcapsules has been achieved and that the alginate/chitosan microcapsule protected the VCA from degradation at acidic pH values. And coating the VCA with polyacrylic polymers, Eudragit, produced outstanding results with ideal release profiles and only minimal losses of cytotoxicity after manufacturing step. The granules prepared with extract or whole plant produced the best results due to the stability in the extract or whole plant during manufacturing process.