• International Journal of Oral Biology
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• 제33권1호
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• pp.33-43
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• 2008

In the process of bone remodeling, mineral phase of bone is dissolved by osteoclasts, resulting in elevation of calcium concentration in micro-environment. This study was performed to explore the effect of high extracellular calcium ($Ca{^{2+}}_e$) on mineralized nodule formation and on the expression of progressive ankylosis (Ank), plasma cell membrane glycoprotein-1 (PC-1) and osteopontin by primary cultured mouse calvarial cells. Osteoblastic differentiation and mineralized nodule formation was induced by culture of mouse calvarial cells in osteoblast differentiation medium containing ascorbic acid and ${\beta}$-glycerophosphate. Although Ank, PC-1 and osteopontin are well known inhibitors of mineralization, expression of these genes were induced at the later stage of osteoblast differentiation during when expression of osteocalcin, a late marker gene of osteoblast differentiation, was induced and mineralization was actively progressing. High $Ca{^{2+}}_e$(10 mM) treatment highly enhanced mRNA expression of Ank, PC-1 and osteopontin in the late stage of osteoblast differentiation but not in the early stage. Inhibition of p44/42 MAPK activation but not that of protein kinase C suppressed high $Ca{^{2+}}_{e^-}$induced expression of Ank, PC-1 and osteopontin. When high $Ca{^{2+}}_e$(5 mM or 10 mM) was present in culture medium during when mineral deposition was actively progressing, matrix calcifiation was significantly increased by high $Ca{^{2+}}_e$. This stimulatory effect was abolished by pyrophosphate (5 mM) or levamisole (0.1-0.5 mM), an alkaline phosphatase inhibitor. In addition, probenecid (2mM), an inhibitor of Ank, suppressed matrix calcification in both control and high $Ca{^{2+}}_{e^-}$treated group, suggesting the possible role of Ank in matrix calcification by osteoblasts. Taken together, these results showed that high $Ca{^{2+}}_e$ stimulates expression of Ank, PC-1 and osteopontin as well as matrix calcification in late differentiation stage of osteoblasts and that p44/42 MAPK activation is involved in high $Ca{^{2+}}_{e^-}$induced expression of Ank, PC-1 and osteopontin.

• 국제학술발표논문집
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• The 3th International Conference on Construction Engineering and Project Management
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• pp.1128-1133
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• 2009

Recently super complex culture shopping facility development seeks consumer' convenience present and are coming restaurant neighborhood, cinema, shopping, hotel etc, according to intensive plan. Such as complex culture shopping facility was developed to most subway station area center and have concept that is space for a main facilities or space for environment protection, citizens' a rest in city. Howeve,r space of recently domestic large size complex culture shopping facility that do not plan systematically was lacking and caused result that do not use efficiently space. Limited extent of research that define complex culture shopping equipment and analyze form of space and present space planning with analysis of research connected with complex usage development.

• International Journal of Advanced Culture Technology
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• 제7권4호
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• pp.156-162
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• 2019

Rheumatoid arthritis (RA) is a chronic autoimmune disorder characterized by progressive joint deterioration; Furthermore, RA can also affect body tissues, including the skin, eyes, lungs, heart and blood vessels. The early stages of RA can be difficult to diagnose because the signs and symptoms mimic those of many other diseases. It is not known exactly what triggers the onset of RA and how to cure the disease. But recent discoveries indicate that remission of symptoms is more likely when treatment begins early with strong medications known as disease-modifying anti-rheumatic drugs (DMARDs). Tumor necrosis factor (TNF) inhibitors are typical examples of biotherapies that have been developed for RA. The substances may occur naturally in the body or may be made in the laboratory. Other biological therapies care biological response modifiers (BRMs)such as monoclonal antibodies, interferon, interleukin-2 (IL-2) and a protein binder using repeat units. These substances play significant anti-inflammatory roles. Proteins with recurrent, conserved amino acid stretches mediate interactions among proteins for essential biological functions; for example, ankyrin (ANK), Heat repeat protein (HEAT), armadillo repeat protein (ARM) and tetratricopeptide repeats (TPR). Here, we describe Leucine rich repeats (LRR) that ideally fold together to form a solenoid protein domain and is more applicable to our current study than the previously mentioned examples. Although BRMs have limitations in terms of immunogenicity and effector functions, among other factors, in the context therapeutic use and for proteomics research, We has become clear that repeat-unit-derived binding proteins will increasingly be used in biotechnology and medicine.

• 한국수산과학회지
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• 제28권4호
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• pp.457-468
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• 1995

현재 유수식 방법으로 양식되고 있는 넙치(Paralichthys olivaceus)의 육상수조 양식에서 환수율, 월동시가온 및 양식배수 등의 문제를 해결하기 위하여, 실제 양식장에 설치한 반폐쇄식 순환여과 사육시스템을 이용하여 1992년 2월부터 1994년 1월까지 2년 동안(실험 I , 실험II) 넘치를 사육하면서 그 실용성을 연구하였다. 실험 I에서는 평균전장 7.5cm, 평균체중 3.4g, 실험II에서는 각각 5.0cm, 1.8g의 종묘를 사용하였다. 실험 I의 사육수 용존 무기태질소량은 $NH_4-N\;0.247-0.512ppm,\;NO_2-N\;0.010-0.043ppm,\;NO_3-N\;0.108-0.342ppm$ 실험II에서는 각각 0.091-0.715ppm, 0.002-0.045 ppm, 0,007-0.277 ppm 범위에서 변화하였다. 일간섭식률은 실험 I $0.67-2.41\%$, 실험II $0.69-2.22\%$, 사료효율은 각각 $34.8-59.8\%,\;40.5-88.4\%$였다. 실험 I에서의 어체는 사육 340일만에 전장 40.0-42.8cm, 체중 695.0-852.6g으로 성장하였고, 실험II에서는 사육 365일후에 전장 36.7-39.7cm, 체중 552.4-706.4g으로 자라났다. 사육종료시 어체의 평균 생존율은 실험 $192.0\%$, 실험II $96.0\%$였다. 어체의 체표면적이 수조바닥을 덮는율(covering rate)은 넙치의 수용밀도의 지표로 활용 가능하였다. 사육시 어체의 최대 수용량은 실험 I에서 덮는율 2.2, 중량 $34.1kg/m^2$였고, 실험II에서는 각각 $2.6,\;36.3kg/m^2$였다. 순환여과식 양식시스템은 현행의 유수식 양식시스템에 비해 양식 생산성 및 연안 환경오염 방지에 기여도가 높은 양식방법으로 평가되었다.

• 미생물학회지
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• 제33권2호
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• pp.149-156
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• 1997

Pentachlorophenol(PCP)가 첨가된 혐기성 무기배지에 혐기성 소화조슬럿지와 쓰레기 매립장의 침출수를 각각 접종한 후, 활성을 보이기 전과 후의 각 시료 내에 존재하는 총유전물질로부터 16S rRNA 유전자를 PCR 증폭하여 이들에 대한 restriction fragment length polymorphism(RFLP) 비교분석과 계통수분석을 실시하였다. 3차에 걸친 RFLP 분석 결과 Ala와 Bld로 명명된 두 가지의 FRLP 유형이 두 가지의 활성시료 모두에서 높은 빈도로 발견되었다. 이들 유형에 속하는 모든 클론들의 5'쪽에 해당되는 180개씩의 염기서열분석을 실시하여 계통수 분석을 실시한 결과, 각각의 유형에 속하는 클론들간에는 높은 상동성을 가지는 것으로 확인되었다. 특히 Bld 유형에서는 78%에 해당되는 클론들이 동일한 염기서열을 가지는 것으로 확인되었다. 이들 Ala와 Bld 유형에 속하는 클론들은 PCP에 대한 분해활성의 결과로서 증식된 유사종의 미생물 군집에서 비롯된 것으로 추정된다. 이 두 가지 유형에 속하는 클론들 중 하나씩의 16S rDNA 전체으 염기서열을 분석한 결과 Ala의 클론은 Clostridium ultunae (Genbank No. Z69293)의 염기서열과 89%의 상동성을 보였으며, Bld의 클론은 Thermobacteroides proteolyticus (Genbank No. X69335)의 것과 97%의 상동성을 가지는 것으로 나타났다.