• Journal of Haehwa Medicine
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• v.15 no.2
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• pp.239-246
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• 2006

Objective : To research the trend of studies about anti-allergic response on Herbal Acupuncture therapy by analysing domestic theses, published after 2001, about Herbal Acupuncture therapy. Methods : I reviewed and analyzed domestic theses, published after 2001, mentioning anti-allergic response of Herbal Acupuncture therapy. And these theses were classified by university, year, field of study, subject. Results : In theses focusing on anti-allergic effect, there were some cases trying to prove remedial value of operating herbal acupuncture on acupuncture points, and cases trying to prove its effect on immunity.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.1
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• pp.76-95
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• 2002

Allergic rhinitis and allergic dermatitis are common allergic diseases. Galgeun-tang has been used for treating various diseases, which include allergic rhinitis and allergic dermatitis. Experimental sutdies have been done to research the anti-allergic effects of Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang. We have observed: the vascular permeability response induced by serotonin and histamine, the contact dermatitis response induced by picryl chloride, the delayed type hypersensitivity response to SRC, the mice paw edema induced by carrageenin, and the rectal temperature in febrile rats induced by endotoxin, as well as the writhing syndrome induced by $0.7{\%}$ acetic acid. The results were as follows: 1. In the vascular permeability response to intradermal serotonin and histamine, Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 2. In the contact dermatitis response induced by picryl chloride, Galgeun-tang and Gamigalgeun-tang showed a signigicant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 3. In the delayed type hypersensitivity response to SRC, Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang showed a significant inhibitory effect. 4. In the mice paw edema induced by carrageenin, Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect. 5. In the rectal temperature in febrile rats induced by endotoxin, Galgeun-tang, Gamigalgeun-tang and Geomahwanggalgeun-tang showed a significant inhibitory effect. 6. In the writhing syndrome induced by a $0.7{\%}$ acetic acid solution. Galgeun-tang and Gamigalgeun-tang showed a significant inhibitory effect. However Geomahwanggalgeun-tang showed an insignificant inhibitory effect.

• The Korea Journal of Herbology
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• v.28 no.6
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• pp.1-7
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• 2013

Objectives : The root of Peucedanum japonicum Thunberg (Peucedani Japonici Radix; PJR) has been traditionally used as an herbal medicine for the treatment of anti-headache, anti-paralysis, anti-cancer, vascular protection, and blood pressure regulation. In this study, we investigated the anti-allergic effect of PJR water extract on ovalbumin (OVA)-induced allergic asthma in mice. Methods : Mice were sensitized at days 1, 8 and 15 with OVA and airway challenged at days 22, 24, 26, 28, and 30 to induced allergic asthma. PJR-W extract at doses of 100 and 300 mg/kg/body weight (bw) was orally administered during OVA challenge once per a day. The levels of allergic mediators such as immunoglobulin (Ig) E, and Th1/Th2 cytokines (IFN-${\gamma}$ and IL-4) were measured in the sera of mice by ELISA. The histological change of lung tissue was observed with hematoxylin and eosin (H&E) staining. Results : The administration of PJR-W extract significantly decreased the serum levels of IgE, IL-4, and IFN-${\gamma}$ compared with those of OVA control group. In H&E staining, PJR-E extract inhibited OVA-induced airway inflammation and the inflammatory cells infiltration in the peribronchial regions of the lung. Conclusions : These results indicate that PJR-W extract has an anti-inflammatory and anti-allergic effect on allergic response through the down-regulation of allergic mediators, suggesting that this herb may be used as a useful source for the treatment of allergic inflammatory diseases such as asthma.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.15 no.1
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• pp.343-355
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• 2002

Background : Although Samsoeum has been used frequently on allergic rhinitis, but there isn't any experimental research for that. Objective : This study was performed to investigate the anti-allergic effects of Samsoeum and Samsoeumgamibang. Materials and Methods : Katayama's method was used to observe the vascular permeability response induced by serotonin and histamine. Muller's method was used to observe the contact dermatitis response induced by picryl chloride. Miller's method was used to observe the delayed type hypersensitivity response to SRC. Results : 1. In the vascular permeability response to intradermal injection of serotonin, 2,600mg/kg, p.o. group of Samsoeum(蔘蘇飮), 1,300 and 2,600mg/kg, p.o. group of Samsoeumgamibang(蔘蘇飮加味方) showed significant inhibitory effects on the leakage of Evan's blue solution. 2. In the vascular permeability response to intradermal injection of histamine, 1,300 and 2,600mg/kg, p.o. group of Samsoeum, also 1,300 and 2,600mg/kg, p.o. group Samsoeumgamibang showed significant inhibitory effects on the leakage of Evan's blue solution. 3. In the contact dermatitis response induced by picryl chloride, 1,300 and 2,600mg/kg, p.o. group of Samsoeum, 1,300 and 2,600mg/kg, p.o. group of Samsoeumgamibang showed significant inhibitory effects on ear swelling formation. 4. In the delayed type hypersensitivity response to SRC. 2,600mg/kg, p.o. group of Samsoeum and 2,600mg/kg, p.o. group of Samsoeumgamibang showed significant inhibitory effects on foot swelling. Conclusion : This study shows that Samsoeum and Samsoeumgamibang may have anti-allergic effects. So Samsoeum and Samsoeurngamibang can be helpful to treat allergic rhinitis.

• The Korea Journal of Herbology
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• v.25 no.3
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• pp.35-41
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• 2010

Objectives : Allergy is an immune dysfunction caused by degranulation from mast cells in the early phase of allergic disease including allergic rhinitis (AR). The purpose of this study was to investigate the effects of Osterici Radix, roots of Ostericum koreanum Maximowicz in human mast cells and experimental allergic animal models. Methods : The anti-allergic effect of Osterici Radix water extract (NK-W) was investigated in human mast cell line, HMC-1 cells, and compound 48/80-induced systemic anaphylactic response in rats and ovalbumin (OVA)-induced AR in mice. Animals were orally administrated with NK-W (10 and 50 mg/kg) or anti-histamine drug, dosodium cromoglycate (50 mg/kg), and then intraperitoneally injected with compound 48/80 (8 mg/kg) or sensitized with 0.1% OVA into nasal. Animals were observed plasma histamine and histological changes of nasal mucosa. Also, mast cell degranulation and histamine production were determined in compound 48/80-stimulated HMC-1 cells. Results : NK-W inhibited compound 48/80-induced degranulation of mast cells and histamine releasing in HMC-1 cells. NK-W decreased mortality and serum histamine releasing in compound 48/80-induced anaphylatic rats in a dose-dependently manner. NK-W also inhibited serum histamine levels in OVA-induced AR mice and improved abnormal histological changes such as expansion of grandular cells and hypertrophy of epithelium in the nasal mucosa. These results indicate that Osterici Radix water extract suppress allergic response through downregulation of mast cell activation. Conclusions : This study suggests that a therapeutic potential of Osterici Radix as a source of anti-allergic agents for use in a number of allergic diseases.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.14 no.2
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• pp.154-172
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• 2001

Major symptoms of al1ergic rhinitis are nasal obstruction, sneezing and watery rhinorrhea. Onpyetang has been used to treat for nasal obstruction, which is one of the symptoms of allergic rhinitis. This Experimental study was done to research effects of Onpyetang and transformed Onpyetang(Allii Radix is deducted from Onpyetang) on the anti-allergic effects. We have studied the vascular permeability response induced by serotonin and histamine, the contact dermatitis response induced by picryl chloride, the delayed type hypersensitivity response to SRC, the mice paw edema induced by carrageenin, the writhing syndrome induced by $0.7\%$ acetic acid, and the rectal temperature in febrile rats induced by yeast. The results were as follows : 1. In the vascular permeability response to intradermal serotonin and histamine. Onpyetang proved significant inhibitory effect(P<0.05, p<0.001) But transformed Onpyetang proved significant inhibitory effect only to histamine(P<0.05) 2. In the contact dermatitis response induced by picryl chloride, Onpyetang and transformed Onpyetang proved significant inhibitory effect.(P<0.05, P<0.05) 3. In the delayed type hypersensitivity response induced by SRC, Onpyetang and transformed Onpyetang proved significant inhibitory effect on mice paw edema.. (P<0.05, P<0.05) 4. In the delayed type hypersensitivity response induced by SRC, Onpyetang proved significant inhibitory effect to serum IgE.(P<0.01) But transformed Onpyetang proved insignificant inhibitory effect on serum Ig E. 5. In the mice paw edema induced by carrageenin, Onpyetang and transformed Onpyetang proved significant anti-inflammatory effect. (P<0.01, P<0.01) 6. In the writhing syndrome induced $0.7\%$ acetic acid, Onpyetang and transformed Onpyetang proved significant analgestic effect. (P<0.01, P<0.01) 7. In the rectal temperature in febrile rats induced by yeast, Onpyetang and transformed Onpyetang proved significant anti-pyretic effect.(P<0.001, P<0.01) according to this result Onpyetang was conclude to be effective on anti-allergic, anti-pyretic, anti-inflammatory and analgestic action. but transformed Onpyetang(Allii Radix is deducted from Onpyetang) was not effective on the vascular permeability response to intradermal serotonin and increasing Ig E of delayed type hypersensitivity response induced by SRC. In addition, transformed Onpyetang is not effective as Onpyetang. More study should be done about the role of Allii Radix.

• Journal of Acupuncture Research
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• v.20 no.4
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• pp.180-191
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• 2003

Objective & Methods : Experimental studies were done to research the clinical effects of Juglandis Semen aqua-acupuncture and acupuncture($BL_{13}{\cdot}BL_{17}$) on the anti-allergic response. anaphylaxis provoked by the compound48/80, delayed type hypersensitivity response to picryl chloride and SRBC and inflammation response to egg albumin. Results : The following results have been obtained; 1. Juglandis Semen aqua-acupuncture and acupuncture($BL_{13}{\cdot}BL_{17}$) group were increased the survival rate in compound 48/80 induced systemic anaphylactic reaction. 2. Picryl chloride induced contact dermatitis and delayed type hypersensitivity in SRBC challenged mouse were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture($BL_{13}{\cdot}BL_{17}$) group. 3. Inflammation response-WBC, CRP and Nitric Oxide in egg albumin induced allergic rat were significantly decreased in Juglandis Semen aqua-acupuncture and acupuncture($BL_{13}{\cdot}BL_{17}$) group. Conclusions : According to the above results, Semen aqua-acupuncture and acupuncture($BL_{13}{\cdot}BL_{17}$) both depress the allergy reaction.

• Journal of Microbiology and Biotechnology
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• v.28 no.10
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• pp.1626-1634
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• 2018

Ginseng (the root of Panax ginseng Meyer) fermented by Lactobacillus plantarum has been found to attenuate allergic responses in in vitro and in vivo experimental models. Ginseng has been reported to also possess various biological functions including anti-inflammatory activity. The present study was aimed at comparing the anti-allergic effect of ginseng and fermented ginseng extracts on IgE-mediated passive cutaneous anaphylaxis in vitro in a murine cell line and in vivo in mice. Fermented ginseng extract (FPG) showed higher inhibitory effect against in vitro and in vivo allergic responses when compared with ginseng extract (PG). The secretion of ${\beta}$-hexosaminidase and interleukin (IL)-4 from the IgE-DNP-stimulated RBH-2H3 mast cells were significantly (p < 0.05) inhibited by FPG treatment, and this effect was concentration-dependent. Further, MKK4 activation and subsequent JNK phosphorylation were attenuated by FPG treatment. The inhibitory effect of FPG on the in vitro allergic response was verified in vivo against IgE-DNP-induced passive cutaneous anaphylaxis in a mouse model. These data indicated that the fermentation of ginseng with L. plantarum enhanced its anti-allergic effects both in vitro and in vivo. We predict that compositional changes in the ginsenosides caused by the fermentation may contribute to the change in the anti-allergic effects of ginseng. The results of our study highlight the potential of the use of FPG as a potential anti-allergic agent.

• Korean Journal of Acupuncture
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• v.25 no.1
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• pp.197-211
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• 2008

Objectives : We studied on anti-allergic effects of Arctii Fructus Herbal Acupuncture(AFHA) and Arctii Fructus Herbal Acupuncture Solution(AF). Methods : In vivo, Animals were herbal-acupunctured AFHA at both ST36 three times for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylaxis(ASA) using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis(PCA) using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase, IL-4 and TNF-a release from RBL-2H3 cells after treatment of AF of various concentrations. Results : In vivo, AFHA pretreatments at both ST36 inhibited compound 48/80-induced ASA. PCA was inhibited by AFHA pretreatments at both ST36. In vitro, AF treatments were not affect on cell viability and inhibited b-hexosaminidase, IL-4 and TNF-a release. Conclusions : These results suggest that AFHA and AF may be beneficial in the inhibition of allergic inflammatory response.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.12 no.1
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• pp.36-46
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• 1999

The mast cell plays a pivotal role in initiating allergic response by secreting intracytoplasmic granular mediators such as histamine. Magnoliae flos has been used for treatment of allergic disease in Korea. However, its effect in experimental models remains unknown. The present report describes an inhibitory effect of Magnoliae flos on mast cell-mediated immediate-type allergic reactions. Topical application of compound 48/80 can induce an ear swelling response in normal($WBB6F_1-+/+$) mice but not in the congenic mast cell-deficient $WBB6F_1-W/W^v$ mice. Magnoliae flos inhibited concentration-dependently mast cell-dependent ear swelling response induced by compound 48/80 by topical application. Magnoliae flos inhibited concentration-dependently passive cutaneous anaphylaxis induced by anti-dinitrophenyl (DNP) IgE in rats by topical application. Magnoliae flos also inhibited concentration-dependently the histamine release from the rat peritoneal mast cells by compound 48/80 or anti-DNP IgE. Moreover, Magnoliae flos had a significant inhibitory effect on compound 48/80-induced systemic anaphylactic reaction. These results indicate that Magnoliae flos inhibits immediate-type allergic reactions by inhibition of mast cell degranulation in vivo and in vitro.