• Title, Summary, Keyword: anti-proliferation

Search Result 1,361, Processing Time 0.033 seconds

Anti-proliferation effect of Gyulyupsanbyonbang extracts on MCF-7 cells (귤엽산변방(橘葉散變方)이 유방암 세포주 MCF-7 생장 억제에 미치는 영향)

  • Yang, Seong-Jung;Cho, Seong-Hee;Cho, Su-In;Jo, Huyn-Jung
    • The Journal of Korean Obstetrics and Gynecology
    • /
    • v.20 no.1
    • /
    • pp.50-60
    • /
    • 2007
  • Purpose : This investigation was undertaken to evaluate the anti-proliferation, in hexane, chloroform, ethyl acetate, butanol and water fraction from extract of Gyulyupsanbyonbang(GYSB) using MCF-7 human breast cancer cells. Methods : GYSB was added to distilled water(1500ml) and was boiled then filtered. The residue was suspended in distilled water and extracted with hexane, chloroform, ethyl acetate, butanol and water. MCF-7 cells were cultured in RPMI1640 complex badge, NIH3T3 was cultered in 37$^{\circ}$C, 5% moisture incubator of carbon dioxide with Dulbecco's Modified Eagle Medium(DMEM) supplemented with 10% fetal bovine serum and antibiotics. Cell cytotoxicity test about cancer cell was measured used MTT assay. Results: When it synthesizes a result, hexane and butanol fraction had shown anti-proliferation effect and safety together, and those anti-proliferation effect operating selectively appeared. Ethyl acetate fraction had anti-proliferation effect however, it was not selective. The Chloroform and water soluble fraction did not almost appear anti-proliferation effect. Conclusion : I can conclude that GYSB have anti-proliferation effect and safety together on MCF-7 cells. It suggest that GYSB may be useful for brest cancer patients.

  • PDF

Anticancer Effects of Ganjang with Different Aging Periods (숙성 기간에 따른 간장의 항암 효과)

  • Hur, Jinyoung;Kim, Min Jung;Hong, Sang Pil;Yang, Hye Jeong
    • Journal of the Korean Society of Food Culture
    • /
    • v.35 no.2
    • /
    • pp.215-223
    • /
    • 2020
  • Ganjang and doenjang are known as major fermented soy-based foods in Koreans. Current investigations have proved that fermented soybean foods impart anti-cancer, anti-obesity, and anti-diabetic effects. The aim of this study was to evaluate the anti-cancer activities of commercialized soy food, Ganjang, as a function of aging period. The test groups were classified into four time periods-short (under 5 years, S group), mid (under 10 years, M group), long (under 15 years, L group), and eternal (over 15 years, E group). The anti-cancer effects of Ganjang were determined by cell cytotoxicity assay of three types of cancer cell lines and splenocyte proliferation assay. Besides these assays, we also analyzed NK cell activity for cancer immunotherapy. The results show that the anti-cancer effect increased in the S and M period aging groups for all three cancer cell lines. Interestingly, similar to the anti-cancer result, splenocyte proliferation and NK activity showed the highest effect in the S and M groups. In contrast, Japanese ganjang-treated (JG1, JG2) groups and E group showed significantly reduced splenocyte proliferation. Collectively, these results suggest that the short and middle periods of traditional fermented Ganjang might have potential anti-cancer activities.

Fucoidan Extract from Laminaria religiosa Suppresses Ischemia-induced Apoptosis and Cell Proliferation in the Hippocampus of Gerbils

  • Lee, Jong-Jin;Song, Yun-Kyung;Lim, Hyung-Ho
    • The Journal of Korean Medicine
    • /
    • v.27 no.4
    • /
    • pp.105-115
    • /
    • 2006
  • Fucoidan has been shown to exhibit a host of biological activities, including anti-coagulant, anti-thrombotic, anti-tumourigenic, anti-inflammatory, anti-viral, anti-complementary and neuroprotective effects. In the present study, we attempted to determine the effects of Fucoidan on both apoptosis and cell proliferation in the hippocampal CA1 region and the dentate gyrus of gerbils after the induction of transient global ischemia. This experiment involved the use of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay as well as immunohistochemisty for caspase-3 and 5-bromo-2'-deoxyuridine (BrdU). The monosaccharide composition of the purified Fucoidan which had been extracted from Laminaria religiosa was utilized in this study. The present study clearly induces that apoptotic cell death and cell proliferation in the gerbil's hippocampal regions increased significantly following the induction of transient global ischemia and the results of this study also indicate that Fucoidan exerted a suppressive effect on this observed ischemia-induced increase in apoptosis within the CA1 and dentate gyrus, and also suppressed cell proliferation in the dentate gyrus.

  • PDF

Study of Takliikgi-tang on the Anti-Cancer Action in Mice (탁리익기탕(托裏益氣湯)이 항암(抗癌) 및 면역기능(免疫機能)에 미치는 작용(作用) 연구(硏究))

  • Park, Su-Yeon;Kim, Jong-Han;Choi, Jung-Hwa;Moon, Oong-A
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.19 no.1
    • /
    • pp.31-42
    • /
    • 2006
  • Objective : This Study was to investigate effects of Takliikgi-tang on the anti-cancer and proliferation of immunocytes, nitric oxide (NO) production of peritoneal macrophages. Methods : We used Takliikgi-tang extract (TLT) with freeze-dried, 8weeks-old male mice and cancer cell lines (L1210, Sarcoma-180) for this Study. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay (MTT assay). Results : 1. TLT was significantly showed cytotoxicity on the L1210, 5-180 cell lines. 2. TLT was significantly increased proliferation of thymocytes in vitro. 3. TLT was significantly increased proliferation of thymocytes and splenocytes in normal mice. 4. TLT was significantly increased NO production from peritoneal macrophages in normal mice. 5. TLT was significantly decreased proliferation of L1210 cells in L1210 cells transplanted mice. 6. TLT was significantly increased proliferation of thymocytes and splenocytes by all-dosage in L1210 cells transplanted mice. 7. TLT was significantly increased NO production from peritoneal macrophages in L1210 cells transplanted mice. Conclusions : The present author thought that TLT had action of anti-cancer by becoming immunocytes activity (NO production, proliferation of thymocytes).

  • PDF

Anti-inflammatory and Immunomodulatory Effects of the Botanical Product $AMP-365^{TM}$ (천연물제제 마루플랜트$-AMP-365^{TM}$의 항염증 및 면역활성에 미치는 영향)

  • Shin, Eun-Myoung;Kim, Dong-Hyun;Kwon, Young-Bok;Kim, Yeong-Shik
    • Korean Journal of Pharmacognosy
    • /
    • v.37 no.3
    • /
    • pp.212-216
    • /
    • 2006
  • The effect of aqueous extracts of medicinal plants $AMP-365^{TM}$ was tested for immune system regulating activity based on anti-inflammatory activity, anti-oxidant, macrophage proliferation and T-lymphocyte proliferation activity. $AMP-365^{TM}$ dose-dependently increased proliferation of RAW264.7 macrophage cells and its nitric oxide production as well as DPPH radical scavenging activity. On the other hand, T-lymphocyte proliferation activity was decreased on dose-dependent manner. Passive cutaneous anaphylaxis reaction was alleviated by 49% by administering 250 mg/kg of $AMP-365^{TM}$. The results suggest that $AMP-365^{TM}$ can be beneficial in the treatment of immediate allergic reactions as an adjuvant supplement material.

Platelets Induce Proliferation of Human Umbilical Vein Endothelial Cells via CD154-CD40 Pathway Independently of VEGF

  • Cho, Wha-Jung;Ko, Eun-Mi;Cheon, In-Su;Jeoung, Doo-Il;Kim, Young-Myeong;Choe, Jong-Seon
    • IMMUNE NETWORK
    • /
    • v.8 no.3
    • /
    • pp.75-81
    • /
    • 2008
  • Background: Platelets take part in repairing the lesions of endothelial damage. To understand the molecular mechanism of this process, we tested the hypothesis that CD154 expressed on activated platelets stimulates proliferation of human endothelial cells. Methods: The expression levels of CD154 and CD40 on platelets and endothelial cells, respectively, were measured by flow cytometry and confocal microscopy. Function-blocking monoclonal antibody against CD154 was developed after immunization with CD154-transfected L cells. Results: An anti-CD40 agonist antibody and soluble CD154 both induced significant proliferation of endothelial cells. In addition, a function-blocking anti-CD154 antibody inhibited the platelet-induced proliferation of endothelial cells, indicating that the CD154-CD40 pathway is involved in these cellular interactions. An anti-VEGF antibody failed to inhibit the proliferation. This, in addition to the fact that very small amounts of VEGF are released from platelets or endothelial cells, suggests that VEGF does not play an important role in the platelet-stimulated proliferation of endothelial cells. Conclusion: Our results indicate that platelets induce proliferation of endothelial cells by CD154-CD40 interactions independently of VEGF.

Mechanism Study of Takli-San on the Anti-Cancer Action in Mice (탁이산(托裏散)이 항암(抗癌) 미치는 작용기전(作用機轉) 연구(硏究))

  • Choi, Jung-Hwa;Kim, Jong-Han;Park, Su-Yeon;Yu, Mi-Kyung
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.18 no.1
    • /
    • pp.71-81
    • /
    • 2005
  • Objective : This Study was to investigate effects of Takli-San on the anti-cancer and proliferation of immunocytes, nitric oxide(NO) production of peritoneal macrophages. Methods : We used Takli-San extract(TLS) with freeze-dried, 8wks-old male mice and cancer cell lines(L120, Sarcoma-180) for this Study. The cytotoxicity and proliferation of cells were tested using a colorimetric tetrazoliun assay(MTT assay). Results : 1. TLS was significantly showed cytotoxicity on the L1210 cell lines. 2. TLS was significantly increased proliferation of thymocytes and splenocytes in vitro. 3. TLS was significantly increased proliferation of thymocytes by all-dosage, but proliferation of splenocytes by low-dosage in normal mice. 4. TLS was significantly increased NO production from peritoneal macrophages in normal mice. 5. TLS was significantly decreased proliferation of L1210 cells in L1210 cells transplanted mice. 6. TLS was significantly increase proliferation of thymocytes by all-dosage, but proliferation of splenocytes by low-dosage in L1210 cells transplanted mice. 7. TLS was significantly increased NO production from peritoneal macrophages in L1210 cells transplanted mice. Conclusions : The present author thought that TLS had action of anti-cancer by becoming immunocytes activity(NO production, proliferation of thymocytes).

  • PDF

Anthocyanins from the Fruit of Vitis Coignetiae Pulliat Inhibit TNF-Augmented Cancer Proliferation, Migration, and Invasion in A549 Cells

  • Lu, Jing Nan;Panchanathan, Radha;Lee, Won Sup;Kim, Hye Jung;Kim, Dong Hoon;Choi, Yung Hyun;Kim, GonSup;Shin, Sung Chul;Hong, Soon Chan
    • Asian Pacific Journal of Cancer Prevention
    • /
    • v.18 no.11
    • /
    • pp.2919-2923
    • /
    • 2017
  • Objective: Anthocyanins belong to a class of flavonoids, exhibiting antioxidant and anti-inflammatory actions have been reported to have anti-cancer effects. Here, we investigated whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in human lung cancer A549 cells, which are critically involved in cancer metastasis. Methods: We used anthocyanins from fruits of Vitis coignetiae Pulliat (AIMs) which has been used in Korean folk medicine for the treatment of inflammatory diseases and cancers. We have performed cell proliferation assays, cell invasion assay, gelatin zymography, wound healing assay and western blotting to examine whether anthocyanins can inhibit cancer cell proliferation, invasion, and angiogenesis in A549 cells. Result: AIMs did not inhibit cancer cell proliferation on A549 cells. Also, AIMs suppressed cancer migration, and invasion by supressing MMP-2 and MMP-9 expression. The Immuno-blotting results also revealed that AIMs suppressed the proteins involved in cancer proliferation (COX-2, C-myc, cyclin D1), migration and invasion (MMP-2, MMP-9), anti-apoptosis (XIAP, and c-IAP2), adhesion and angiogenesis (ICAM-1, VEGF). Conclusion: This study demonstrates that the anthocyanins isolated from fruits of Vitis coignetiae Pulliat inhibit cancer proliferation, cancer migration, and invasion that is involve in cancer-metastasis. This study provides evidence that AIMs might have anti-cancer effects on human lung cancer.

Effects of Acanthopanax sessiliflorus on Immune Cells such as Thymocytes, Splenocytes and Macrophages in Mice

  • Kim, Hyung-Woo;Kim, Gye-Yeop;Jeon, Byung-Gwan;Choi, Jeong-Sik;Jeong, Hyun-Woo;Cho, Su-In
    • The Journal of Internal Korean Medicine
    • /
    • v.28 no.2
    • /
    • pp.377-384
    • /
    • 2007
  • Objective : Immune potentiation including activation of T cells, B cells, macrophages, and dendritic cells is known to play a key role in prevention and treatment of patients with cancer. In this study, we investigated the effects of Acanthopanax sessiliflorus (AR) on the immune system, especially on thymocytes, splenocytes, and macrophages. Methods : We investigated the effects of AR on proliferation of splenocytes in normal mice, and the effects on proliferation of splenocytes and thymocytes in tumor-bearing mice. In addition, the effect of AR on NO production using macrophages was investigated. Results : Treatment with AR accelerated proliferation of splenocytes in vitro. AR also accelerated thymocyte proliferation, but did not affect splenocytes proliferation in normal mice. In contrast, AR accelerated proliferation of splenocytes and thymocytes significantly in tumor bearing mice. In addition, NO production level from macrophages was elevated by treatment with AR. Conclusion : These results demonstrate that AR has anti-cancer activities and related mechanisms are involved in immune potentiation such as acceleration of immune cell proliferation and elevation of NO production level in macrophages. In addition, we also demonstrate the possibilities of AR as complementary and alternative medicine to standard anti-cancer drugs.

  • PDF

Effects of Neasookseul-Tang on Anti-Cancer Action in Mice (내소옥설탕(內消沃雪湯)의 항암효과(抗癌效果))

  • Ko, Hong-Gae;Kim, Jong-Han;Park, Su-Yeon;Choi, Jung-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
    • /
    • v.18 no.1
    • /
    • pp.82-93
    • /
    • 2005
  • Objective: This Study was to investigate effects of Neasookseul-Tang on the anti-cancer, proliferation of immunocytes and nitric oxide(NO) production of peritoneal macrophages. Methods: We used Neasookseul-Tang extract(NOT) with freeze-dried, 8wks-old male mice(balb/c, ICR) and cancer cell lines(L1210, sarcoma-180) for this Study. The proliferation of cells was tested using a colorimetric tetrazoliun assay(MTT assay). Results: 1. NOT was significantly showed cytotoxicity on the L1210 and Sarcoma-180 cell lines. 2. NOT was significantly increased proliferation of thymocytes and splenocytes in vitro. 3. NOT was significantly decreased proliferation of L1210 cells in L1210 cells transplanted mice. 4. NOT was significantly increased proliferation of thymocytes and splenocytes in L1210 cells transplanted mice. 5. NOT was significantly increased NO production from peritoneal macrophages in L1210 cells transplanted mice. 6. NOT was significantly inhibited body weight and tumor weight in Sarcoma-180 cells transplanted mice. 7. NOT was significantly increased in the mean survival days in Sarcoma-180 cells transplanted mice. Conclusions: The present author thought that NOT had action of anti-cancer by accelerating immuno-function.

  • PDF