• Title/Summary/Keyword: antimicrobial activity

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Functional Finishing of Cotton Fabrics by Treatment with Chitosan

  • Shin, Youn-Sook;Min, Kyung-Hye;Jang, Jeong-In
    • Proceedings of the SOHE Conference
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    • 1997.12a
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    • pp.33-37
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    • 1997
  • Cotton fabric was treated with chitosan solution by pad-dry(-cure) method to impart antimicrobial properties. Four chitosans of different degree of deacetylation(DAC: 65~95%) with similar molecular weight(ca. 50, 000) and one chitosan oligomer(MW 1, 800, DAC 86%) were used. Antimicribial activity against Staphylococcus aureus was evaluated by the Shake Flask Method. Treated fabrics were laundered up to 20 times according to AATCC Test Method 60-1986 and antimicrobial activity of laundered fabrics was evaluated. The antimicrobial activity was increased with the concentration and DAC of chitosan used. And the cured samples showed better durability to laundering than not-cured samples. Crosslinker and binder decreased the antimicrobial activity of fabrics treated with chitosan oligomer and were not effective to improve the durability to washing.

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Study on Antimicrobial Activity of Extracts from Fritillaria unibracteata Hsiao et K.C. Hsia and F. ussuriensis Maxim.

  • Moon, Jung-Wook;Chen-Zhi, Chen-Zhi;Song, Won-Seob;Baek, Seung-Hwa
    • Korean Journal of Plant Resources
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    • v.24 no.6
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    • pp.719-723
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    • 2011
  • Antimicrobial activities of methanol, ethanol, water, and $CH_2Cl_2$ extracts from Fritillaria unibracteata Hsiao et K.C. Hsia and F. ussuriensis Maxim. were investigated by disk-agar diffusion method. The result showed comparatively strong antimicrobial activity against several microorganisms. The extracts from F. unibracteata and F. ussuriensis dosedependently increased the activity. However, water and $CH_2Cl_2$ extracts showed no antimicrobial activity against 7 microorganisms. Especially, against the most sensitive microorganism Staphylococcus epidermidis, methanol extracts at highest concentration of 20 mg/mL exhibited the largest clear zone on plate by 6-12 mm and ethanol extracts on plate by 6-10 mm.

Antimicrobial Activity of Korean Medicinal Plants and Herbal Formulations

  • Bhatt, Lok-Ranjan;Woo, Shin-Sun;Yu, Byung-Soo;Baek, Seung-Hwa
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.3
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    • pp.755-759
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    • 2007
  • In this study, we screened twenty four extracts of eight medicinal plants and three extracts of the commercial product for their antimicrobial and antioxidant properties. Ethanol and ethyl acetate extracts were found active where as aqueous extracts were little or no active. Extracts of Sophora flavescens, Salvia miltlorrhiza and Glycyrrhiza uralensis showed strong activity againsttested organisms and could be the potential antimicrobial agent. The increase of antimicrobial and antioxidant activities of formulations might be due to synergic effect. The results also indicated that the activity of bamboo salt and herbal products can be enhanced by making appropriate formulations.

Antioxidative and Antimicrobial Activities of Essential oil from Artemisia vulgaris

  • Bhatt Lok Ranjan;Lim Jin-A;Chai Kyu-Yun;Kang Jeong-Il;Oh Hong-Keun;Baek Seung-Hwa
    • Natural Product Sciences
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    • v.12 no.4
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    • pp.226-231
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    • 2006
  • Artemisia vulgaris, one of the most religious plants in Nepal, is used in the treatment of various ailments. In this study, antioxidative activity of essential oil from A. vulgaris was evaluated, using different in vitro methods and antimicrobial activity by disc diffusion method against skin disease microorganisms such as Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Candida albicans and Propionibacterium acnes. The essential oil exhibited a concentration-dependent antioxidant activity. It showed strong metal chelating activity with low reducing and antioxidant power. However, gradual increase in radical scavenging activity was obtained with increasing concentration and reaction time. It also possessed a broad spectrum of antimicrobial activity and notable susceptibility was observed against S. pyogenes and P. acnes.

Antimicrobial activity of protein hydrolysate by protease (효소 단백 가수분해물의 항균 활성)

  • Joo, Jeong-Hyeon;Yi, Sang-Duk;Lee, Jeong-Ok;Oh, Man-Jin;Rhee, K.C.
    • Korean Journal of Agricultural Science
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    • v.29 no.2
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    • pp.78-90
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    • 2002
  • This study was carried out to investigate whether peptide produced from wheat protein by enzyme hydrolysis can be used as a natural antimicrobial agent. Antimicrobial peptide was obtained from wheat protein by protease of 7 species. The produced antimicrobial peptide was purified through ultrafiltration, membrane filtration and HPLC, and molecular weight and amino acid sequence of the purified antimicrobial peptide were determined. Among hydrolysate produced from wheat protein by protease of 7 species, antimicrobial activity was observed for the peptide obtained from Asp. saito protease. The Asp. saito protease did production antimicrobial hydrolysate showing the highest antimicrobial activity at reaction condition of $37^{\circ}C$ and pH 6.0, but not at reaction condition above $50^{\circ}C$. Wheat protein hydrolysate was fractionated by membrane filtration and showed antimicrobial activity between molecular weight 1,000 - 3,000. The antimicrobial activity fraction obtained by membrane filtration was separated through HPLC and showed antimicrobial activity in the peak of retention time 31.1 - 31.8 min. Since after wheat protein protease hydrolysate was heated during 15 min at $121^{\circ}C$, antimicrobial activity was maintained, we could be conviction as heat-stable peptide. Molecular weight of antimicrobial peptide identified by MALDI-mass was 1,633. Amino acid sequence of antimicrobial peptide was cysteine, glycine, prolin, prolin, prolin, valine, valine, alanine, alanine and arginine.

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Antimicrobial Activity of Natural Quercetin Alone and in Combination with Some Antibiotics (천연 퀘루세틴이 수종 항생물질의 항균력에 미치는 병용효과)

  • Eo, Seong-Kug;Kim, Young-So;Lee, Chong-Kil;Lee, Do-Ik;Kim, Il-Hyuk;Han, Seong-Sun
    • YAKHAK HOEJI
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    • v.40 no.6
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    • pp.653-658
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    • 1996
  • As part of our search for less toxic antimicrobial agents from natural resources. rutin was isolated from Sophora japonica and then hydrolyzed to quercetin. Antimicrobial activity of quercetin was tested in vitro against five kinds of gram positive and ten kinds of gram negative bacteria by serial broth dilution method. Among fifteen kinds of bacteria tested, the antimicrobial activity of quercetin was the most potent against Proteus vulgaris showing minimal inhibitory concentration(MIC) of 125 ${\mu}$g/ml. To investigate the effect of antimicrobial combinations of quercetin with four kinds of antibiotics (ampicillin, cefazolin, oxytetracycline and chloramphenicol). the fractional inhibitory concentration index (FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of quercetin with four kinds of antibiotics resulted in synergism in one instance, additive effect in four instances, but no antagonism was observed.

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Antimicrobial and Antioxidant Peptide from Bacillus Strain CBS73 Isolated from Korean Food

  • Kim, Miri;Khan, Md Maruf;Yoo, Jin Cheol
    • Journal of the Chosun Natural Science
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    • v.10 no.3
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    • pp.154-161
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    • 2017
  • An antimicrobial peptides-producing Bacillus strain CBS73 was isolated from fermented food (kimchi) that produces low-molecular-weight proteins with broad-spectrum antimicrobial activity. Our goal was to explore the therapeutic potential of antimicrobial substances produced by Bacillus species. Peptide CBS73 was purified from Bacillus subtilis subsp. subtilis with identity of 99.79%. It was found to be stable at pH 4.0-10.0 and temp $20-60^{\circ}C$. A protein band around 5.2 kDa was detected in tricine-SDS-PAGE and band was confirmed by MALDI-TOF test. Peptide CBS73 showed antimicrobial activity against MDR bacteria. The minimal inhibitory concentration (MIC) of peptide CBS73 for vancomycin-resistant S. aureus (VRSA), vancomycin resistant Enterococci (VRE) and Salmonella typhimurium ranged from $10-40{\mu}g/mL$. The antioxidant activity of peptide CBS73 was measured by DPPH scavenging, reducing power activity and total phenolic content. Cell viability and NO production result showed less cytotoxic effect upto $12{\mu}g/mL$. Peptide CBS73 could be a promising antimicrobial agent for clinical application.

Antimicrobial and Antioxidant Activities of Extracts of Marine Green-algae Enteromorpha intestinalis (거대녹조류 창자파래 추출물의 항균, 항산화 활성)

  • Kim, Dong-Hyun;Jeong, Gwi-Taek
    • KSBB Journal
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    • v.29 no.2
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    • pp.92-97
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    • 2014
  • In this study, we investigate the antimicrobial and antioxidant activities of extracts of Enteromorpha intestinalis. To evaluate the antimicrobial and antioxidant activities, three solvents (hexane, chloroform and methanol) were applied to obtain extracts. The extraction yields are hexane (A) 1.11%, chloroform (B) 0.94%, and methanol (C) 8.2%. Also, the contents of total phenolic compounds of extract A, B, C are 4.03%, 8.15%, and 2.33%, respectively. In the results of antimicrobial activity, extract A and B have higher activity than that of extract C. Especially, Vibrio vulificus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli are more sensitive than others. In antioxidant activity, extract A and B present the higher DPPH activity than that of control (BHA and ascorbic acid). The DPPH radical scavenging activity and SOD-like activity are ordered as B>C>A. However, extract C show high value in ferric reducing antioxidant power assay. In nitrite scavenging activity, extract A is relatively higher than others. However, the antioxidant activities are lower than that of controls (ascorbic acid and BHA). The antioxidant activities are presented the increasing pattern of increasing by the increasing of extract concentration.

Relationship between Chemical Structure and Antimicrobial Activities of Isothiocyanates from Cruciferous Vegetables against Oral Pathogens

  • Ko, Mi-Ok;Kim, Mi-Bo;Lim, Sang-Bin
    • Journal of Microbiology and Biotechnology
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    • v.26 no.12
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    • pp.2036-2042
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    • 2016
  • We evaluated the potentials of 10 isothiocyanates (ITCs) from cruciferous vegetables and radish root hydrolysate for inhibiting the growth of oral pathogens, with an emphasis on assessing any structure-function relationship. Structural differences in ITCs impacted their antimicrobial activities against oral pathogens differently. The indolyl ITC (indol-3-carbinol) was the most potent inhibitor of the growth of oral pathogens, followed by aromatic ITCs (benzyl ITC (BITC) and phenylethyl ITC (PEITC)) and aliphatic ITCs (erucin, iberin, and sulforaphene). Sulforaphene, which is similar in structure, but has one double bond, showed higher antimicrobial activity than sulforaphane. Erucin, which has a thiol group, showed higher antimicrobial activity than sulforaphane, which has a sulfinyl group. BITC and iberin with a short chain exhibited higher antimicrobial potential than PEITC and sulforaphane with a longer chain, respectively. ITCs have strong antimicrobial activities and may be useful in the prevention and management of dental caries.

Antimicrobial Activity of the Ethanol Extract from Rubus coreanum against Microorganisms Related with Foodborne Illness (복분자 에탄올 추출물의 식중독 관련 위해 세균에 대한 항균활성 분석)

  • Jeon, Yeon-Hee;Sun, Xiaoqing;Kim, Mee-Ra
    • Korean journal of food and cookery science
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    • v.28 no.1
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    • pp.9-15
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    • 2012
  • This study analyzed the antibacterial activity of a Rubus coreanum (Bokbunja) ethanol extract. The antimicrobial activity was determined by disc diffusion, minimum inhibitory concentration (MIC), and growth inhibition methods with seven kinds of bacteria related to foodborne illness (Bacillus cereus, Escherichia coli, Escherichia coli O157:H7, Listeria monocytogenes, Pseudomonas aeruginosa, Staphylococcus aureus, and Salmonella typhimurium). In the results, disc diffusions of the ethanol extract from R. coreanum (9.8-17.5 mm at $4,000{\mu}g/disc$) clearly showed the antimicrobial activity of the extract against all tested microorganisms. Rubus coreanum promoted an inhibitory effect as follows: E. coli O157:H7 > P. aeruginosa > L. monocytogenes > E. coli > S. aureus > B. cereus ${\geq}$ S. typhimurium. In the MIC test, R. coreanum showed high antimicrobial effect against L. monocytogenes at 500 ppm. Moreover, the R. coreanum ethanol extract showed strong growth inhibition against microorganisms, similar to the MIC results. These results show that a R. coreanum ethanol extract has powerful antimicrobial activity against all tested microorganisms, suggesting that R. coreanum will be useful as a potential natural preservative.