• Asian-Australasian Journal of Animal Sciences
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• 제27권2호
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• pp.278-283
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• 2014

Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic, CecP4 cDNA was prepared and cloned into the pPICZ B expression vector and followed by the integration into AOX1 locus in Pichia pastoris. The supernatants from cell culture were collected after methanol induction and concentrated for the test of antimicrobial activity. The recombinant P. patoris having CecP4 showed antimicrobial activity when tested against Staphyllococcus aureus in disc diffusion assay. We selected one of the CecP4 clones (CecP4-2) and performed further studies with it. The growth of recombinant P. pastoris was optimized using various concentration of methanol, and it was found that 2% methanol in the culture induced more antibacterial activity, compared to 1% methanol. We extended the test of antimicrobial activity by applying the concentrated supernatant of CecP4 culture to Pseudomonas aeruginosa and E. coli respectively. Recombinant CecP4 also showed antimicrobial activity against both Pseudomona and E. coli, suggesting the broad spectrum of its antimicrobial activity. After improvements for the scale-up, it will be feasible to use recombinant CecP4 for supplementation to the feed to control microbial infections in young animals, such as piglets.

• 한국지역사회생활과학회지
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• 제15권1호
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• pp.85-90
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• 2004

To classify the physiological activity by habitat and part of Dandelion (Taraxacum officinale), antioxidative and antimicrobial activities were investigated from methanol extracts of Dandelion. Dandelion was taken from Uiryeong (in Kyungnam province) and from Seoul, and antioxidative and antimicrobial activities were measured for three different parts of the dandelion: the flower, leaf and root. Antioxidative activities of methanol extracts from Dandelion were examined by a DPPH test, and antimicrobial activities were examined against 5 microorganisms: Bacillus subtilis and Bacillus cereus as G(+) microorganisms, and Salmonella typhimurium, Staphylococcus aureus and Eschrichia coli as G(-) microorganisms. No significant differences were found in terms of antioxidative and antimicrobial activities between the dandelions in Uiryeong and the dandelions in Seoul. Antioxidative activity, however, was higher in the flower of the dandelion, irrespective of habitat. Further research with other physiological factors (besides antioxidative and antimicrobial activities) is necessary to shed light on dandelion physiological function and to enhance the utilization of dandelion.

• 대한의생명과학회지
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• 제13권3호
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• pp.189-195
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• 2007

This study was carried out to examine antimicrobial substances from medicinal plants, the ethanol extracts of 38 medicinal plants were tested for the antimicrobial activity against Staphylococcus aureus ATCC 25923 and methicillin-resistant Staphylococcus aureus ATCC 43300. The extracts of Glycyrrhiza uralensis, Sophora flavescens, Dryopteris crassirhizoma, and Pinas densiflora showed significant antimicrobial activities against both S. aureus ATCC 25923 and methicillin-resistant S. aureus ATCC 43300. The extract of Dryopteris crassirhizoma among these medical plants showed the highest antimicrobial activity. These results suggested that the extracts from Dryopteris crassirhizoma, Sophora flavescens, Pinas densiflora, and Glycyrrhiza uralensis could be the potential source of antimicrobial agents against methicillin-resistant S. aureus and S. aureus.

• 한국세라믹학회지
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• 제54권3호
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• pp.228-234
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• 2017

This work was achieved on the preparation of antimicrobial ceramic hybrid polyethylene films with natural chamomile extracts. The antimicrobial activity and various physicochemical properties of the prepared films were evaluated. Various natural products demonstrated antimicrobial activity. Among them, chamomile extracts showed strong activity and no cytotoxicity rather than that of the natural extracts. Porous ceramic materials were synthesized and demonstrated loading and controlled release of natural antimicrobial extracts. Furthermore, chamomile loaded ceramic hybrid films showed antimicrobial activity that was maintained for over 15 days.

• 한국식품영양과학회지
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• 제27권5호
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• pp.993-999
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• 1998

Methanol extract of roots of Amblytropis pauciflora Kitagawa showed the antimicrobial activity to three test strains. Antimicrobial spectra of various extracts of Amblytropis pauciflora Kitagawa were tested against 24 strains of bacteria and fungi. The crude methanol extract inhibited the growth of 12 strains of bacteria and Asp. fumigatus with the exception of yeasts. The properties of the antimicrobial substance were very stable under heat(at 12$0^{\circ}C$), acid(pH 3.0) and alkali(pH 11.0) treatment. Only the root harvested in spring showed the antimicrobial activity. Among the components extracted by butanol, ginseng saponin Rg1 and various saponin-like materials were detected by TLC analysis using a plate of silica gel 60F254. The antimicrobial compound was purified by methanol extraction, activated charcoal column chromatography, Sep-pak(C18) pretreatment and reverse phase HPLC. The purified compound was detected at 13.520 min as a single peak(about 98% purity) through the HPLC analysis.

• 한국정보통신학회:학술대회논문집
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• 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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• pp.980-981
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• 2008

This study was carried out to research antimicrobial agents from medicinal plants, Lonicera japonica, Pinellia ternata, Dictamnus albus, Cryptotympana pustulata, Pinus densiflora, Bupleurum falcatum, Forsythia saxatilis, Castanea crenata, Hovenia dulcis, Prunus sargentii. The ethanol extracts of 10 medicinal plants were tested for the antimicrobial activity against Streptococcus mutans. The extracts of Pinus densiflora showed significant antimicrobial activity against Streptococcus mutans. These results suggested that the extract from Pinus densiflora could be a candidate for new antimicrobial agents against Streptococcus mutans.

• KSBB Journal
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• 제27권2호
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• pp.114-120
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• 2012

To investigate the antimicrobial activity on bacteria causing a respiration disease and antioxidant effects of water extracts from 12 kinds of wild edible vegetables, we extracted the water extracts for 72 h in $7^{\circ}C$ using distilled water as solvent. The water extracts except Ixeris dentate and Allium monanthum had high concentrations of phenol compounds and flavonoids. Liguraia fischeri specially had the highest level on total phenol compounds and flavonoids with 205 ${\mu}g/mL$ and 98. 86 ${\mu}g/mL$, respectively. The each 0.05% extracts of Sedum sarmentosum and Liguraia fischeri had high effect on the DPPH radical scavenging activity among wild edible vegetables and the most extracts promoted antioxidant activity with increasing concentration of extract. The catalase activity of Erysimum aurantiacum and Aralia elata showed more than 150 units per g of fresh tissue. The effect of antimicrobial activity on water extracts showed characteristic activity. Only Staphylococcus aureus KCTC 1928 and Corynebacterium diptheriae KCTC 3075 were inhibited cell growth on the other hands, the remainder of bacteria was not inhibited cell growth. Nevertheless, the extracts of wild edible vegetables had specific concentration as MIC for antimicrobial activity respectively. In case of the extract of Aster scaber, Erysimum aurantiacum, and Allium monanthum had over 30% antimicrobial activity on the bacteria causing a respiration disease. In results, the wild edible vegetables include high concentrations of total phenol compounds and flavonoids that give a good antioxidant activity and antimicrobial activity. Therefore the wild edible vegetables are functional food for anti-aging and physiological activation.

• 대한한의학회지
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• 제41권2호
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• pp.1-8
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• 2020

Objectives: To establish the expiration date of herbal decoction pouch, the change in antimicrobial activities was measured according to storage temperature and period. Methods: Five herbal formulas, Gyeji-tang, Jakyakgamcho-tang, Saengmaek-san, Paljung-san, and Dangguisu-san, with antimicrobial activity were selected from 51 herbal formulas commonly used in traditional Korean medical institutions. Five herbal formula decoction pouches were prepared and stored at room temperature (RT) or 4℃ from 0 to 12 weeks to evaluate the antimicrobial activity against Bacillus cereus, Listeria monocytogenes, or Vibrio parahaemolyticus. The antimicrobial activities were evaluated by the disk diffusion method (disk size: 10 mm). Results: The decoction pouches of Gyeji-tang and Jakyakgamcho-tang maintained antimicrobial activity against V. parahaemolyticus from week 0 (14 mm) to week 12 (14 mm) at both temperatures. Saengmaek-san pouch had antimicrobial activities against V. parahaemolyticus and B. cereus from week 0 (19 mm and 20 mm) to week 12 (both 18 mm), respectively. At RT and 4℃, the antimicrobial activity of Paljung-san pouch was maintained week 0 (17 mm) to week 12 (both 13 mm) against B. cereus. Against L. monocytogenes, the antimicrobial activity of Dangguisu-san pouch showed 27 mm at week 0 and 23 mm (RT) and 24 mm (4℃) at week 12, respectively. Conclusion: According to increase of the storage duration, the antimicrobial activities of 5 herbal formula decoction pouches decreased slightly but remained up to 12 weeks. These results can be used as important data to establish the expiration date of herbal formula decoction pouches.

• 한국식품영양과학회지
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• 제32권5호
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• pp.745-751
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• 2003

밀 단백질에 효소가수 분해할 때 생산되는 peptide의 항균활성과 천연항균제로서의 이용가능성을 검토하기 위하여 실험을 행하였다. 밀 단백질에 7종의 단백질가수분해효소를 작용시켜 생성된 가수분해물의 항균활성을 측정하고 한외여과, membrane filtration, HPLC를 이용하여 항균성 peptide를 분리 정제한 후 분자량과 아미노산 결합순서를 측정한 결과는 다음과 같다. 밀 단백질에 7종의 단백질 분해효소를 적용시켜 제조한 가수분해물중 Asp. saito protease를 적용시켜 얻어진 peptide만이 항균활성을 나타내었다. Asp. saito protease는 37$^{\circ}C$, pH 6.0에서 작용시킨 경우에 항균활성이 가장 높았으며, 5$0^{\circ}C$ 이상에서는 활성을 나타내지 않았다. 밀단백 효소가수분해물은 membrane filtration에 의하여 분자량 1,000~3,000에서 항균활성이 나타났다. Membrane filtration으로 얻어진 항균활성분획을 HPLC로 분리한 결과 retention time 31.1~31.8 min에서 항균활성을 나타내었다. 밀단백 효소가수분해물은 121$^{\circ}C$에서 15분간 가열하여도 효소활성이 유지되는 매우 안정한 화합물이었다. 항균활성분획을 MALDI-mass로 질량을 분석한 결과 1,633이었다. 항균성 peptide의 아미노산 결합순서는 cysteine, glycine, prolin, valine, valine, alanine, alanine, arginine의 순서였다.

• 공업화학
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• 제20권3호
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• pp.251-257
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• 2009

다양한 미생물에 대하여 높은 불활성화 성능을 지니고 있는 은(Ag)은 최근 환경 기술 분야, 나노 기술 분야 등에서 응용 가능성이 높아 큰 주목을 받고 있으며, 새로운 적용 제품들이 활발하게 연구, 개발되고 있다. 하지만 다양한 응용 연구에도 불구하고 정확한 항균 성능 및 기작에 대한 연구 결과와 이해가 부족하여 관련 연구자와 소비자들에게 논쟁과 혼동을 야기시키고 있다. 본 글에서는 기존 연구를 중심으로 은의 미생물 불활성화 성능과 기작, 다른 항균 물질과의 시너지 효과, 응용 분야 등에 대해서 정리, 검토하여 이에 대한 연구 및 개발에 도움이 되고자 한다.