• Title, Summary, Keyword: antioxidant activity

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Antioxidant Activity from the Stem Bark of Albizzia julibrissin

  • Jung, Mee-Jung;Chung, Hae-Young;Kang, Sam-Sik;Choi, Jin-Ho;Bae, Kae-sun;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.26 no.6
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    • pp.458-462
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    • 2003
  • The antioxidant activity of the stem bark from Albizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical ($\cdot OH$), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites ($ONOO^{-}$). The methanol extract of A. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) > n-butanol (n-BuOH) > dichloromethane ($CH_2 CI-2$) > and water ($H_2O$). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3 ,4 ,7-trihydroxyflavone (2) were isolated as the active principles. Compounds 1 and 2 exhibited good activity in all tested model systems. Compound 1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound 2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of the A. julibrissin crude extract and its major constituents.

Antioxidant and Antimicrobial Activities of Xanthium sibiricum (창이자의 항산화 활성 및 항균효과)

  • Shin, Sun-Woo;Lee, Jeong-Ho;Soo, Bang-Keuk
    • Korean Journal of Plant Resources
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    • v.25 no.4
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    • pp.372-378
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    • 2012
  • Xanthium sibiricum using extracts antioxidant activity and antimicrobial activity were analyzed and the following results were obtained. Antioxidant activity using DPPH as a VLC column fractionation experiments XS-5 (water fraction of Xanthium sibiricum) concentration in the 500 ${\mu}g/ml$ to 87.53 percent in the highest antioxidant activity was found, VLC fractionation in XS-3-2 (eluted with chloroform/methanol 7~10%) concentration of 500 ${\mu}g/ml$, showed the antioxidant activity of 78.72%. The antioxidant activity measured by PCL XS-1 (n-hexane fraction of Xanthium sibiricum) XS-5 (water fraction of Xanthium sibiricum) and 13.32 nmol open column XS-3-2 (eluted with chloroform/methanol 7~10%) showed antioxidant activity with 14.34 nmol. The antimicrobial activity against Candida albicans XS-2 (methlene chloride fraction fo Xanthium sibiricum) is the concentration of 500 ${\mu}g$/disc 1 mm of the clean zone was formed. XS-3 (ethanol fraction fo Xanthium sibiricum) in the antioxidant activity using DPPH and PCL had higher antibacterial activity was low.

Antioxidant Activity of Amino Acid-Xylose Browning Reaction Products 1. Antioxidant Activity of Various Amino Acids and Their Browning Reaction Products (Amino산-Xylose 갈변반응 물질의 항산화성 1. 아미노산과 갈변 반응 물질의 항산화성)

  • YOU Byeong-Jin;LEE Kang-Ho;KIM Chang-Yang;LEE Jong-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.19 no.1
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    • pp.1-9
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    • 1986
  • In order to isolate and clarify the antioxygenic substances from the browning reaction products, the antioxidant activity of various amino acids and their browning reaction products were measured when they were reacted with xylose. Among nonpolar amino acids Met and Trp appeared to have stronger antioxidant effect than others. Most of polar and basic amino acids, however, did not have antioxidant activity. Ser and Cys showed a rather slight prooxidant effect. The browning reaction products of Trp and His had a higher level of antioxidant activity than that they were reacted as free amino acids. But the browning product of Met did not show the antioxidant activity. When all amino acids were divided on their polar characteristics, the higher optical density of the browning reaction products showed, the stronger antioxidant activity revealed.

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Effect of Ethanol Solvent Concentration on Antioxidant Activity of Dolwoe (Gynostemma pentaphyllum Makino) Leaves Extracts (돌외 잎 추출물의 항산화 활성에 미치는 에탄올 용매 농도의 영향)

  • Kim, Kyeoung Cheol;Kim, Ju-Sung
    • Journal of Advanced Engineering and Technology
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    • v.11 no.3
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    • pp.197-203
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    • 2018
  • The purpose of this study was to the extraction availability of Gynostemma pentaphyllum leaves on different ethanol concentration extraction solvent were compared and analyzed. We prepared extracts of different solvents. Total phenol and flavonoid contents of each extract were measured. These extracts were evaluated for antioxidant activity by measuring trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant power (FRAC), oxygen radical absorption capacity (ORAC), DPPH, nitric oxide, superoxide and hydroxyl radical scavenging activity. The total phenol contents of extracts was 44.16~66.34 mg QAE/g, and the total phenol content in the extracts was significantly increased when ethanol concentration of 60% or more was used. Total flavonoid content was 6.98~16.89 mg QE/g and 60, 80% ethanol extracts were significantly higher. In the antioxidant experiments, 60% ethanol extracts showed high activity in nitric oxide, superoxide radical scavenging activity, TEAC and ORAC which showed correlation with total phenol and flavonoid contents. The optimal ethanol concentration used for the extraction of antioxidant-related components from Gynostemma pentaphyllum leaves is considered to be about 60%, antioxidant activity was thought to be related to total phenol and flavonoid contents.

Chlorogenic Acid, an Antioxidant Principle from the Aerial Parts of Artemisia iwayomogi that Acts on 1,1-Diphenyl-2-picrylhydrazyl Radical

  • Kim, Soon-Shin;Lee, Chung-Kyu;Sam, Sik-Kang;Jung, Hyun-Ah;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.20 no.2
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    • pp.148-154
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    • 1997
  • The antioxidant activity of Artemisia iwayomogi was determined by measuring the radical scavenging effect on 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical. The methanol extract of A. iwayomogi showed strong antioxidant activity, and thus fractionated with several solvents. The antioxidant activity potential of the individual fraction was in the order of ethyl acetate > n-butanol > water > chloroform > n-hexane fraction. The ethyl acetate and n-butanol soluble fractions exhibiting strong antioxidant activity were further purified by repeated sitica get and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid was isolated as one of the active principles from the n-butanol fraction, together with the inactive components, 1octacosanol, scopoletin, scopolin, apigenin $7, 4^{I}$-di-O-methylether, luteolin $6, 3^{I}$-di-O-methylether (jaceosidin), apigenin methylether (genkwanin), 2, 4-dihydroxy-6-methoxyacetophenone $4-O-{\beta}-$D-glucopyranoside and quebrachitol. The antioxidant activity of chlorogenic acid was comparable to that of L-ascorbic acid, which is a well known antioxidant.

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Antioxidant Activity and Total Phenolic Compounds in Grain Extracts of Wheat, Barley, and Oat

  • Seo, Yong-Weon;Bu, So-Young;Jeon, Woong-Bae;Kim, Dong-Sub;Heo, Hwa-Young
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.2
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    • pp.102-107
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    • 2002
  • Cereal grains are rich in phenolic compounds that give beneficial effect in human health. Although several research works have been reported on the effects of phytochemicals of plant origin, such as fruits, vegetables, few studies have examined the antioxidative effects of whole cereal grains. The objective of this study was to determine total antioxidant capacity of 80% ethanolic extracts of cereal grains by testing the ability of the extracts to inhibit UV -induced lipid peroxidation in vitro using linoleic acid in comparison to well-known antioxidant such as ascorbic acid and tannic acid. The total phenolic content of the cereal grain (80% ethanolic extracts) investigated in this study varied from 2.1 mg/g (wheat cv. Olgeurumil) to 10.4 mg/g (barely cv. Seodunchalbori). Highly positive relationship between total phenol compounds and antioxidant activity was found. When the antioxidant activities of all investigated extracts were measured with application of same quantity of phenol compounds, oat grain extracts showed similar antioxidant activity of barely cultivars. However, barely extract appeared as the most potent antioxidant activity of inhibition of UV -induced lipid peroxidation. This indicated that factors such as phenolic compound composition and their individual antioxidant activity could playa crucial role in the total antioxidant activity of cereal grains.

Antioxidant effect of myricetin with other antioxidants, taurine and $\beta$-carotene on mouse melanoma cell

  • Yu, Ji-Sun;Kim, An-Keun
    • Proceedings of the Korean Society of Applied Pharmacology
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    • pp.69-69
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    • 2003
  • There are now increasing evidences that free radicals and reactive oxygen species are involved in a variety of pathological events. Reactive Oxygen Species (ROS) are produced during normal cellular function. ROS lead to lipid peroxidation, massive protein oxdiation and degradation. Under normal conditions, antioxidant are substnaces that either directly or indirectly protect cell against adverse effect of ROS. several biologically important compound include ${\beta}$-carotene, taruine and flavonoids reported have antioxidant function. The various antioxidant either scavange superoxide and free radicals or stimulate the detoxification mechanisms within cells resulting in increased detoxification of free radicals formation and thus in prevention of many pathophysiologic processes. This study carried out to investigate the antioxidant activity of flavonoids, myricetin with other antioxidants, ${\beta}$-carotene and taurine on B16Fl0. In order to investigate the efficacy of antioxidant activity, we measured cell viability, antioxidant enzyme activity (SOD, GPX, CAT) and intracellular reactive oxygen intermediate (ROI). In this results, we show that these flavonoids with other antioxidant substrates are increased antioxidant activity level.

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A study on the relations between the color intensity and the antioxidant activity of caramelization products (카라멜화 반응 생성물의 갈색도와 항산화 효과와의 관계)

  • 신민자;윤혜현;안명수
    • Korean journal of food and cookery science
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    • v.18 no.6
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    • pp.603-612
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    • 2002
  • The study was carried out to compare the relation between the color intensity and antioxidant activity of caramelization products using xylose(XY), glucose(GL). sucrose(SU), glucose+citric acid(GLCA), glucose+sodium citrate(GLSC), heated at 80, 120 and 140$\^{C}$ for 24hrs, respectively. The color intensity(absorbance at 490nm) of the browning mixtures increased as the browning temperature and time increased. But the degrees of color intensity of SU and GLCA changed very little. The hydrogen donating ability(HDA) of browning reaction products was generally enhanced as the browning temperature and time increased. When browning mixtures were heated at 80$\^{C}$, the HDA of GLGC was the highest, but the HDA of GLSC was the highest when heated at 120 and 140$\^{C}$. The antioxidant activities for the corn oil substrate containing the anhydrous ethanol extracts from the browning mixtures was inferior to that of SU, but was superior to that of GLCA. The relations among the color intensity, the antioxidant activity, and the hydrogen donating ability(HDA, reducing power) of the browning reaction mixtures were as follows: As the color intensity increased, the antioxidant activity decreased. The correlation coefficient of the color intensity and the antioxidant activity by regression equation was -0.73 ∼ -0.82. As the reducing power increased, the antioxidant activity decreased. The correlation coefficient between the reducing power and the antioxidant activity by regression equation was -0.98 ∼ -0.99. Therefore, the antioxidant activity of browning reaction mixtures seemed not correlated with the color intensity and the reducing power.

Antioxidant Flavonoids and Chlorogenic Acid from the Leaves of Erobotrya japonica

  • Jung, Hyun-Ah;Park, Jong-Cheol;Chung, Hae-Young;Kim, Jong;Choi, Jae-Sue
    • Archives of Pharmacal Research
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    • v.22 no.2
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    • pp.213-218
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    • 1999
  • The antioxidant activity of Eriobotrya japonica was determined by measuring the radical scavenging effect on DPPH (1,1-diphenyl-2-picrylhydrazyl) radical and lipid peroxidation produced when mouse liver homogenate was exposed to the air at $37^{\circ}C$, using 2-thiobarbituric acid (TBA). The methanol extract and its factions of Eriobotrya japonica leaves showed strong antioxidant activity. The antioxidant activity of EtOAc and n-BuOH soluble fractions were stronger than the others, and were further purified by repeated silica gel, MCl gel CHP-20P, and Sephadex LH-20 column chromatography. Antioxidant chlorogenic acid, quercetin-3-sambubioside from n-BuOH fraction and methyl chlorogenate, kaempferol- and quercetin-3-rhamnosides, together with the inactive ursolic acid and$ 2{\alpha}$-hydroxyursolic acid from EtOAc fraction were isolated. Antioxidant flavonoids and chlorogenic acid also showed prominent inhibitory activity against free radical generation in dichlorofluorescein (DCF) method.

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Antioxidant Activity and Protective Effects of Extracts from Sambucus williamsii var. coreana on t-BHP Induced Oxidative Stress in Chang cells (접골목 추출물에 의한 항산화 활성이 정상 간세포의 t-BHP 유발 산화스트레스에 미치는 영향)

  • Kim, Kitae
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.17 no.3
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    • pp.275-286
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    • 2013
  • In the present study, antioxidant activity and protective effect of extracts from Sambucus williamsii var. coreana stems (SWC) were evaluated on tert-butyl hydroperoxide (t-BHP) induced oxidative stress in human liver (Chang) cells. Antioxidant activities of the SWC extracts were determined by various radical scavenging activities, such as DPPH, ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assay. SWC extracts showed strong antioxidant effect on various assay. To determine the hepatoprotective effects of SWC on t-BHP induced oxidative damage, cell viability was measured using MTT assay. Pretreatment of SWC extracts showed increasing cell viability, decreasing ROS and restoring mitochondria membrane potential on t-BHP induced oxidative stress in Chang cells. Our findings suggest that SWC extracts may be considered a potential agent for therapeutic protective effect from oxidative stress through its antioxidant activity.