• Title, Summary, Keyword: antioxidant activity

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Antioxidant Activity and Protective Effects of Extracts from Sambucus williamsii var. coreana on t-BHP Induced Oxidative Stress in Chang cells (접골목 추출물에 의한 항산화 활성이 정상 간세포의 t-BHP 유발 산화스트레스에 미치는 영향)

  • Kim, Kitae
    • The Journal of the Society of Korean Medicine Diagnostics
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    • v.17 no.3
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    • pp.275-286
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    • 2013
  • In the present study, antioxidant activity and protective effect of extracts from Sambucus williamsii var. coreana stems (SWC) were evaluated on tert-butyl hydroperoxide (t-BHP) induced oxidative stress in human liver (Chang) cells. Antioxidant activities of the SWC extracts were determined by various radical scavenging activities, such as DPPH, ferric reducing antioxidant power (FRAP), 2,2'-azinobis-(3-ethybenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity and oxygen radical absorbance capacity (ORAC) assay. SWC extracts showed strong antioxidant effect on various assay. To determine the hepatoprotective effects of SWC on t-BHP induced oxidative damage, cell viability was measured using MTT assay. Pretreatment of SWC extracts showed increasing cell viability, decreasing ROS and restoring mitochondria membrane potential on t-BHP induced oxidative stress in Chang cells. Our findings suggest that SWC extracts may be considered a potential agent for therapeutic protective effect from oxidative stress through its antioxidant activity.

Screening for Antioxidant Activity of Plants and Marine Algae and Its Active Principles from Prunus davidiana

  • Choi, Jae-Sue;Lee, Ji-Hyeon;Park, Hae-Jin;Kim, Hyung-Geun;Young, Han-Suk;Mun, Sook-Im
    • Korean Journal of Pharmacognosy
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    • v.24 no.4
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    • pp.299-303
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    • 1993
  • The antioxidant activity of methanol extracts of plants and marine algae was tested by using 1,1-diphenyl-2-picrylhydrazyl(DPPH). Five plant extracts(Prunus davidiana, Eriobotrya japonica, Artemisia iwayomogi, Stirodella tolyrrhiza and Ulmus davidiana) and two algae (Ecklonia stolonifera and Symphycoladia latiuscula) were found to be the most effective in DPPH radical scavenging activity. The methanol extract obtained from the stems of Prunus davidiana was fractionated with several solvents. The ethylacetate soluble fraction exhibiting the strongest antioxidant activity was further purified by repeated silica gel and Sephadex LH-20 column chromatography. Antioxidant flavonoids and flavonoid glycosides were isolated and the most active ones was identified as (+)-catechin by MS, $^1H-NMR$ and $^{13}C-NMR$. Its antioxidant activity was higher than that oil vitamin C.

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Bioactive Compound Contents and Antioxidant Activity in Aronia (Aronia melanocarpa ) Leaves Collected at Different Growth Stages

  • Thi, Nhuan Do;Hwang, Eun-Sun
    • Preventive Nutrition and Food Science
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    • v.19 no.3
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    • pp.204-212
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    • 2014
  • The bioactive compounds and antioxidant activity of aronia leaves at different stages of maturity were identified and evaluated. Young and old leaves were approximately 2 months of age and 4 months of age, respectively. The young leaves contained more polyphenols and flavonoids than the old leaves. Three phenolic compounds (i.e., chlorogenic acid, p-coumaric acid, and rutin) were detected by HPLC. Antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical, and superoxide anion radical scavenging assays. The reducing power of aronia leaf extracts increased in a concentration-dependent manner ($0{\sim}100{\mu}g/mL$). The antioxidant activity of the 80% ethanol extract was greater than that of distilled water extract. The high phenolic compound content indicated that these compounds contribute to antioxidant activity. The overall results indicate that aronia leaves contain bioactive compounds, and that younger aronia leaves may be more favorable for extracting antioxidative ingredients because they contain more polyphenols.

Effect of Red Pepper Seed on Kimchi Antioxidant Activity During Fermentation

  • Sim, Ki-Hyeon;Han, Young-Sil
    • Food Science and Biotechnology
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    • v.17 no.2
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    • pp.295-301
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    • 2008
  • In this study, the antioxidant activities of red pepper seed kimchi extracts were examined. The extracts were evaluated by various antioxidant assays that included determinations of total phenolic and flavonoid contents, DPPH radical scavenging, superoxide anion radical scavenging, nitric oxide scavenging, superoxide dismutase (SOD) activity, metal chelating activity, and reducing power. All the kimchi extracts showed strong antioxidant activities by the tested methods. The 7% red pepper seed kimchi that was fermented for 6 days possessed the highest activity according to the performed tests. Yet, the highest scavenging activity was exhibited against nitrite, by extracts from kimchi for 0 day of fermentation rather than 6 days. All the activities for the kimchi fermented for 0 day with the addition of 7% red pepper seed increased markedly with an increase in concentration. With the exception of metal chelating and SOD activities, for the antioxidant properties assayed, the red pepper seed kimchi extracts were found to be highly effective.

Increasement of Eleutherosides and Antioxidant Activity in Eleutherococcus senticosus Adventitious Root by Jasmonic acid

  • Ahn, Jin Kwon;Park, Youngki;Lee, Wi Young;Park, So-Young
    • Journal of Korean Society of Forest Science
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    • v.96 no.5
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    • pp.539-542
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    • 2007
  • This study was carried out to investigate the impacts of jasmonic acid (JA) on adventitious root culture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid (JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, total phenolic contents and antioxidant activity. Among various concentrations of JA, 1.0 mg/L JA increased the total phenolic contents in E. senticosus adventitious root to $39.81{\mu}g/g$, about 2.6 times higher than that of the control. Consequently, high accumulation of total phenolic contents led to increase the antioxidant activity to 82.41%. The antioxidant activity of control was 37.89% at $2500{\mu}g/mL$. A linear correlation ($R^2$ = 0.9937, 0.9648 and 0.9883) was also shown between antioxidant activity (at 1250, 1875, and $2500{\mu}g/mL$) and total phenolic contents of adventitious root of E. senticosus.

Antioxidant Activity of Low Molecular Weight Laminarin Prepared with Gamma Irradiation (감마선 조사된 저분자 laminarin의 항산화 활성 연구)

  • Choi, Jong-Il;Kim, Hyun-Joo;Lee, Ju-Woon
    • KSBB Journal
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    • v.26 no.6
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    • pp.565-568
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    • 2011
  • In this study, it was investigated the antioxidant activity of laminarin degraded by gamma irradiation. Because the activities of antioxidants have been attributed to various mechanisms, different assay methods have been conducted and compared. 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power, and lipid peroxidation inhibitory activity of degraded laminarin were measured and compared with non-degraded. All of these results showed that the antioxidant activity of laminarin degraded by irradiation was increased depending on the absorbed dose. Therefore, gamma irradiation could be an alternative method for the preparation of degraded laminarin with higher antioxidant activity.

Antioxidant activity of medicinal plant extracts cultivated in Jecheon (제천산 약용식물 추출물의 항산화 특성)

  • Lee, Bo-Ram;Min, Sung-Hee
    • Journal of the Korean Society of Food Culture
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    • v.22 no.3
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    • pp.336-341
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    • 2007
  • Antioxidant activity of Astragalus membranaceus, Polygonatum stenophyllum, Angelica gigas, Acanthopanax sessiliflorus and Angelica pubescens extracts cultivated in Jecheon prepared with different solvents were determined by 1,1-diphenyl-2-picrylhydrzyl(DPPH) radical scarvenging ability and thiocyanate method . The highest hydroxy radical scarvenging activity was shown in Acanthopanax sessiliflorus, while the lowest was in Polygonatum stenophyllum. Angelica gigas showed strong antioxidant activity by thiocyanate method. Methanol extracts and water extracts showed higher antioxidant activities than ethanol extracts. Angelica pubescens showed the highest polyphenol contents and Acanthopanax sessiliflorus showed the highest flavonoid contents. Direct correlation between the hydroxy radical scavenging activity and polyphenol contents or flavonoid contents was established by simple regression(r>0.8) in each solvent extracts. Among medicinal plants cultivated in Jecheon, Acanthopanax sessiliflorus and Angelica pubescens showed strong antioxidant activities. These results suggest that methanol or water extracts of several medicinal plant cultivated in Jecheon could be used as natural antioxidants.

In vitro and Cellular Antioxidant Activity of Arginyl-fructose and Arginyl-fructosyl-glucose

  • Lee, Jung-Sook;Kim, Gyo-Nam;Lee, Sang-Hyun;Kim, Eui-Su;Ha, Kyoung-Soo;Kwon, Young-In;Jeong, Heon-Sang;Jang, Hae-Dong
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1505-1510
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    • 2009
  • Arginyl-fructose (AF) and arginyl-fructosyl-glucose (AFG) were chemically synthesized and purified. Their in vitro and cellular antioxidant activity was investigated using oxygen radical absorbance capacity (ORAC) and cellular antioxidant activity assay, respectively. The peroxyl radical scavenging activity of AF was much higher than that of AFG, which was in good agreement with their reduction capacity to donate electrons or hydrogen atoms. On the other hand, the hydroxyl radical scavenging activity of AF was weaker than that of AFG, which was consistent with their metal chelating activity, suggesting that AFG-$Cu^{2+}$ complex may be less redox-active than AF-$Cu^{2+}$ complex due to 1 glucose molecule attached. The cellular antioxidant activity of AF and AFG appeared to depend on both their permeability into cell membrane and the scavenging activity on peroxyl or hydroxyl radicals. These results indicate that AF and AFG, Maillard reaction products, may have a high potential as a material for the development of nutraceutical food with antioxidant activity.

In Vivo/In Vitro Properties of Novel Antioxidant Peptide from Pinctada fucata

  • Ma, Yongkai;Huang, Kehui;Wu, Yanyan
    • Journal of Microbiology and Biotechnology
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    • v.31 no.1
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    • pp.33-42
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    • 2021
  • Due to the potential of antioxidants to scavenge free radicals in human body, it is important to be able to prepare antioxidant peptides that meet the industrial requirements for cosmetics and food. Here, we determined in vivo/in vitro activities of antioxidant peptide from P. fucata (PFAOP) prepared by bio-fermentation method. The antioxidant property test results showed the DPPH, hydroxyl, superoxide radical-scavenging, and cellular antioxidant activity. EC50 values of PFAOPs were 0.018 ± 0.005, 0.126 ± 0.008, 0.168 ± 0.005, and 0.105 ± 0.005 mg/ml, respectively, exhibiting higher antioxidant activities than glutathione (p < 0.05). Moreover, anti-proliferation and cytotoxicity activity results illustrated PFAOP has a potent anti-proliferative activity against HepG2, Caco-2, and MCF-7 carcinoma cells with no cytotoxicity. Moreover, the protocols we developed in this work demonstrated several excellent advantages in PFAOP preparation compared to enzymatic hydrolysis or chemical synthesis methods and provide a theoretical foundation for higher-value application of marine-derived functional peptides.

Antioxidant Activity of Diarylbutanes

  • Lee, Jung-Yun;Han, Yong-Bong;Woo, Won-Sick;Shin, Kuk-Hyun
    • Korean Journal of Pharmacognosy
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    • v.21 no.4
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    • pp.270-273
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    • 1990
  • Antioxidant activity of diarylbutane type lignans was evaluated in TBA-reactant assay to elucidate the structure-activity relationship. The antioxidant potency of lignans increased with increasing the number of hydroxyl groups, with the exception of macelignan(I), which showed a more potent activity than demethyl meso-dihydroguaiaretic acid(III).

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