• Title/Summary/Keyword: antioxidant compound

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Potential Biological Activities of Magnoflorine: A Compound from Aristolochia debilis Sieb. et Zucc

  • Li, Chunmei;Wang, Myeong-Hyeon
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.223-228
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    • 2014
  • Magnoflorine, an important compound in Aristolochia, was usually used as an anxiolytic chemical. In this study, the magnoflorine was isolated from Aristolochia and the biological activities such as antioxidant, ${\alpha}$-tyrosinase inhibitory, anti-inflammatory, and anticancer activities were investigated. The magnoflorine showed significant antioxidant activity as a 2,2-diphenyl-2-picryl-hydrazyl (DPPH) free radical scavenger, $50{\mu}g/mL$ of the magnoflorine scavenged about 70.8% of all the free radicals. And it was good at ${\alpha}$-tyrosinase inhibiting, $100{\mu}g/mL$ of the magnoflorine inhibited 36.5% of the tyrosinase. High dosage of magnoflorine inhibited the inflammation production nitric oxide (NO), and the magnoflorine protected the murine macrophage cells (RAW 264.7) from LPS-induced apoptosis. The cell viability of human colon cancer calls (HT-29) was around 100% when treated with different dose of magnoflorine, it's suggesting that magnoflorine had no anticancer effect.

A New Antioxidant Polyphenolic Compound from Two Korean Brown Algae

  • Park, Soo-Hee;Kim, Eun-Sook;Choi, Byoung-Wook;Lee, Bong-Ho
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.261.2-262
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    • 2003
  • In the course of our researches for biologically active compound from Korean algae, purification of the methanolic extracts of two brown algae (Sagassum Sagamianum and Ishige Okamurae) collected off Jeju Island afforded an antioxidant polyphenolic compound (1). The molecular formular of 1 was established as C$\sub$24/H$\sub$16/ O$\sub$13/ on the basis of the FAB mass and $\^$13/C NMR spectrum. (omitted)

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Neuroprotective Activity of Lonicerin Isolated from Lonicera japonica (금은화에서 분리한 Lonicerin의 신경세포보호 활성)

  • Lee, Hyunwoo;Ma, Choong Je
    • Korean Journal of Pharmacognosy
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    • v.52 no.1
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    • pp.19-25
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    • 2021
  • We previously reported that lonicerin isolated from Lonicera japonica methanolic extract had potent neuro-protective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of L. japonica extract and lonicerin in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of lonicerin. We used HT22 cell death injured by glutamate as a bioassay system. The compound decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca2+ concentration was decreased by lonicerin treatment. This compound made mitochondrial membrane potential maintain to normal condition. Lonicerin also increased not only glutathione reductase but also peroxidase to the control level. And this compound increased amount of glutathione, an endogenous antioxidant. These results indicated that lonicerin isolated from L. japonica showed potent neuroprotective activity through the anti-oxidative pathway.

Antioxidant effect of myricetin with other antioxidants, taurine and $\beta$-carotene on mouse melanoma cell

  • Yu, Ji-Sun;Kim, An-Keun
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.69-69
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    • 2003
  • There are now increasing evidences that free radicals and reactive oxygen species are involved in a variety of pathological events. Reactive Oxygen Species (ROS) are produced during normal cellular function. ROS lead to lipid peroxidation, massive protein oxdiation and degradation. Under normal conditions, antioxidant are substnaces that either directly or indirectly protect cell against adverse effect of ROS. several biologically important compound include ${\beta}$-carotene, taruine and flavonoids reported have antioxidant function. The various antioxidant either scavange superoxide and free radicals or stimulate the detoxification mechanisms within cells resulting in increased detoxification of free radicals formation and thus in prevention of many pathophysiologic processes. This study carried out to investigate the antioxidant activity of flavonoids, myricetin with other antioxidants, ${\beta}$-carotene and taurine on B16Fl0. In order to investigate the efficacy of antioxidant activity, we measured cell viability, antioxidant enzyme activity (SOD, GPX, CAT) and intracellular reactive oxygen intermediate (ROI). In this results, we show that these flavonoids with other antioxidant substrates are increased antioxidant activity level.

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The Antioxidant Activities of Artemisia spp. Collections (쑥 수집종의 항산화력)

  • Choi, Yong-Min;Chung, Bong-Hwan;Lee, Jun-Soo;Cho, Yong-Gu
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.51 no.spc1
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    • pp.209-214
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    • 2006
  • One hundred Individuals that were collected from plains and mountains all around South Korea were used for this experiment. The inhibition abilities of lipid peroxidation by Artemisia spp. collections were compared with BHT (butylated hydroxytoluene). The results could be confirmed the excellency fur control of lipid peroxide level such as BHT 200 ppm in all mugwort collections. Antioxidant activity (AEAC), electron donating ability (EDA), total phenolic compound, and flavonoids of 100 Artemisia spp. collections were analyzed. Total phenolic compound contents of Artemisia spp. collections were ranged from 156 to 1,767 rng/100 g, and mugwort collections with more than 900 mg/100 g of total phenolic compound content were 20 individuals. Electron donating abilities were ranged from 13.4 to 95.0%, and mugwort collections over 90% of electron donating ability were 23 individuals. Antioxidant activity of ethanol extracts that used ABTS and DPPH radical were measured and mugwort collections with high total phenolic compound contents had high radical exclusion ability as well. Artemisia spp. collections, AC-60, AC-67, AC-77, that showed the high levels of antioxidant activities and had good growth characters and productivity, were selected for mass production.

Study on Antioxidant Activity of Smallanthus sonchifolius, Agrimonia pilosa, and Lithospermum erythrorhizon Extract Fractions (야콘, 선학초, 자초 추출물 분획의 항산화 활성에 관한 연구)

  • Kim, A-Ram;Jeong, Gwi-Taek
    • KSBB Journal
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    • v.30 no.6
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    • pp.302-306
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    • 2015
  • In this work, the antioxidant activity and total phenolic compound content of 6 fractions of Smallanthus sonchifolius, Agrimonia pilosa, and Lithospermum erythrorhizon extract were investigated. The highest total phenolic compound contents of each plant extracts were obtained from n-butanol ($13.75{\pm}0.21%$) and methylene chloride ($12.89{\pm}1.10%$) fractions (S. sonchifolius), ethyl acetate ($19.69{\pm}1.02%$) and water ($18.72{\pm}0.76%$) fractions (A. pilosa), and n-butanol ($36.26{\pm}1.26%$) and ethyl acetate ($17.66{\pm}0.94%$) fractions (L. erythrorhizon), respectively. As a result of DPPH radical scavenging activity in 10 mg/mL condition, the highest activity were obtained from n-butanol fraction of S. sonchifolius (81.06%), ethyl acetate fraction of A. pilosa (86.32%), and n-butanol fraction of L. erythrorhizon (82.6%), respectively. Also, the highest reducing power was obtained same fractions as well as DPPH adical scavenging activity. Overall, antioxidant activity has relatively closely connected with contents of total phenolic compounds in S. sonchifolius and L. erythrorhizon extracts.

Purification and Characterization of Antioxidant Substance from the Stem Bark of Rhus verniciflua (옻나무 껍질에서 항산화물질의 정제와 특성)

  • Kim, Jung-Bae
    • The Korean Journal of Food And Nutrition
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    • v.14 no.6
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    • pp.527-531
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    • 2001
  • In order to isolate antioxidant substances from Rhus verniciflua (RV) , the dried stem bark was extracted with water. The crude water extracts was purified by using HPLC method with a DEAE (anionic type) , CN and ODS column. The purified compound remained stable at pH 3.0∼6.0. but unstable above pH 6.5. It was stable at 100$^{\circ}C$ for 4 hours, but still had about 80% of residual activity after treatment at 100$^{\circ}C$ for 5 hours. In antimicrobial test, no inhibition was observed against Gram-positive and negative bacteria. This compound was stronger than that of commercial antioxidant showed that DPPH test, such as BHT, BHC at the same concentration (20 $\mu\textrm{g}$/ml) .

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Cell Cycle Regulation and Antioxidant Activity of Psammaplin A, A Natural Phenolic Compound from Marine Sponge

  • Jiang, Ya-Hong;Ryu, Seung-Hee;Ahn, Eun-Young;You, Song;Lee, Burm-Jong;Jung, Jee-H;Kim, Dong-Kyoo
    • Natural Product Sciences
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    • v.10 no.6
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    • pp.277-283
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    • 2004
  • Psammaplin A (PSA), a naturally occurring biophenolic compound has been demonstrated to deliver significant cytotoxicity to many cancer cell lines. In this article, we investigated the effect of PSA on cell cycle progression of lung cancer cells (A549). It was found that PSA could slightly perturb the cell cycle progression of A549 cells and lead to the cell cycle arrest at G2/M phase, indicating PSA might disturb the mitosis process of A549 cells. In addition, inspired by the two phenolic groups in the structure of PSA, the antioxidant activity of it has been evaluated. Although PSA was weak in scavenging the stable free radical 1,1-diphenyl-2-picrylhyrazyl (DPPH), it showed stronger ABTS radical scavenging activity than ascorbic acid in TEAC assay. Furthermore, it was found that PSA could effectively prevent DNA strand scission induced by oxidative stress. These results suggest that PSA have both cell cycle regulation and antioxidant activities. Herein, we suggest that PSA would be a very interesting and promising candidate to be developed as a multi-function drug.

Variations in total phenols, total anthocyanins, and antioxidant activity levels in black chokeberry (Aronia melanocarpa) fruits subjected to dry and moist heat treatments

  • Kim, Hekap;Mai, Thu Thi Hoai
    • Korean Journal of Food Science and Technology
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    • v.52 no.5
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    • pp.503-509
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    • 2020
  • The present study investigated the effects of dry and moist heat treatments on total phenols, total anthocyanins, and antioxidant activity levels in black chokeberry (Aronia melanocarpa) fruits. Lyophilized chokeberry powder samples were heated in a drying oven at 60, 100, 160, 180, and 200℃ for 20, 40, or 60 min. Finely ground fresh chokeberry fruits were heated in water at 60, 80, and 100℃ for 20 min, and bioactive compound and antioxidant activity levels were measured. The bioactive compounds and antioxidant activity decreased with increasing temperature and treatment duration. Antioxidant activity was preserved at 160℃ or lower without significant loss for dry heating, whereas moist heat treatment increased both bioactive compounds and antioxidant activity with increasing temperature.

Antioxidant Activity of Stevia Leaf Extracts Prepared by Various Extraction Methods (다양한 추출방법으로 조제된 스테비아 잎 추출물의 항산화 활성)

  • Kim, Jae-Hun;Sung, Nak-Yun;Kwon, Sun-Kyu;Jung, Pil-Moon;Choi, Jong-Il;Yoon, Yo-Han;Song, Beom-Seok;Yoon, Tai-Young;Kee, Hee-Jin;Lee, Ju-Woon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.2
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    • pp.313-318
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    • 2010
  • This study was carried out to evaluate the antioxidant activity of stevia extracts from Stevia rebaudiana Bertoni leaves. Stevia extracts were prepared by three different methods including hot water extraction (HWE) at $120^{\circ}C$ for 4 hr, vacuum extraction (VE) at $65^{\circ}C$ for 4 hr under 0.08 MPa, and fermentation of hot water extract (FHWE) using Lactobacillus buchneri. The antioxidant activities measured by radical scavenging activity, ferric-reducing antioxidant potential ability, and thiobarbituric acid reactive substance showed the highest values in vacuum extract. Also, the antioxidant activities of all extracts were higher than those of stevioside and rebaudioside at the same concentrations, known as the major active components in stevia. To define the antioxidative compound in stevia extracts, the total phenol content was measured, and it was shown that the highest contents of total phenolic compounds were in vacuum extract. These results suggest that the antioxidant activity of stevia extract was due to the phenolic compound components. In addition, vacuum extraction was the proper method to prepare stevia extract with higher antioxidant activity.