• 한국식품영양과학회지
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• 제42권6호
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• pp.851-855
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• 2013

본 연구에서는 시판하는 naringin, rutin 및 morin 등의 이소플라본을 시료로 하여 환원력, 전자공여능, SOD 유사활성 및 hydroxyl radical 소거능 등의 항산화특성을 확인할 수 있는 실험을 행하였으며 이들에 대한 마늘 추출물의 영향을 함께 조사하였다. 항산화능을 측정한 모든 실험항목에서 대조군으로서 사용한 합성항산화제인 BHA보다 훨씬 양호한 결과를 나타내었으며, 이소플라본의 농도가 증가함에 따라서도 다소간 증가하는 것으로 나타났지만 그 차이는 크지 않았다. 특히 마늘을 첨가하였을 때는 그 효능이 더욱 상승하는 것으로 나타났는데 마늘의 첨가량을 늘릴수록 그 효능은 더욱 크게 나타났다. 또한 이소플라본 간의 비교에서는 실험항목 별로 특별한 경향은 찾아볼 수 없었는데 환원력에서는 naringin이, hydroxyl radical 소거효과 및 SOD 유사활성에서는 rutin이, 그리고 전자공여능에서는 morin이 다소 우수한 결과를 나타내었다. 따라서 이소플라본의 항산화적 특성이 매우 뛰어남을 확인할 수 있었으며 이러한 작용은 합성항산화제인 BHA보다 우수하여 기능성 식품가공을 위한 주요 소재로서도 유효하게 이용될 수 있다고 생각된다. 또한 마늘의 섭취가 이소플라본의 항산화적 특성에 상승효과를 제공할 수 있는 것으로 나타나 식생활의 올바른 개선에 중요한 역할을 할 수 있을 것으로 여겨지는 바이다.

• 농업과학연구
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• 제39권1호
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• pp.81-86
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• 2012

In this study, the antioxidative effect and protective potential against oxidative damage of extract and fractions from broccoli were investigated under in vitro and cellular system. The methanol (MeOH) extracts of broccoli flowers and sprouts were partitioned as dichloromethane, n-butanol (BuOH) and aqueous fractions. The comparison of antioxidative effect of broccoli flowers and sprouts showed that broccoli sprouts exerted the more effective protective activity from 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced oxidative stress in LLC-$PK_1$ porcine renal epithelial cell. In addition, the extract and fractions from broccoli sprouts showed strong scavenging effect of 1,1-diphenyl-2-picrylhydrazyl radical and the BuOH fraction exerted the strongest activity. Therefore, the BuOH fraction was evaluated as the most active fraction with strong radical scavenging activity among the fractions of broccoli flowers and sprouts. The present study suggests the antioxidative potential against free radical-induced oxidative damage of flowers and sprouts of broccoli. In addition, the BuOH fraction of broccoli is considered as the active fraction with antioxidative effect.

• 대한수의학회지
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• 제47권1호
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• pp.25-32
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• 2007

Houttuynia cordata root on non-lipid oxidative damage. The antioxidative efects of methanolic (MeOH) extract of Houttuynia cordata rooton non-lipid, including liposome oxidation, oxidation of deoxyribose, protein oxidation, chelating, scavenging,and 2'-deoxyguanosine (2'dG) oxidation were investigated. Houttuynia cordata root exhibited highantioxidative effect in a liposome model system. The inhibitory effect of MeOH extract on deoxyribosedamage exhibited antioxidative effect and it afforded considerable protection against damage to deoxyribose.In addition, MeOH extract at over 300extracts exhibited metal binding ability for hydrogen peroxide. Furthermore, the oxidation of 2'dG to 8-hydroxy-2-deoxyguanosine was inhibited by MeOH extracts, and scavenging activity for hydroxyl radicalexhibited a remarkable effect. The present results on biological model systems showed that MeOH extractswas effective in the protection of non-lipids against various oxidative model systems.

• 한국식품영양학회지
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• 제13권1호
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• pp.53-58
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• 2000

The production of malondiadehyde(MDA) was significantly decreased when the methoanol extract of persimmon leaves was added to the system. The methanol extract of persimmon leaves was fractionated by using various solvents such as hexane, chloroform, ethylacetate and butanol. Among the above fractions especially the chloroform fraction, ethylacetate fraction revealed the strong antioxidative activities. The hot water extract of the persimmon leaves was less effective than tannin which was extracted from persimmon leaves in antioxidative activity.

• 약학회지
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• 제44권4호
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• pp.354-357
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• 2000

Two ellagitannins, pedunculagin and 2,3-(S)-Hexahydroxydiphenoyl (HHDP)-D -glucose and three condensed tannins, (+)-catechin, (-)-epicatechin and procyanidin B-4 which were isolated from Rubus coreanum were evaluated for their antioxidative effects with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and lipid peroxidation generation system mediated by addition of $H_2O$$_2$to rat liver homogenate (TBARS). The ellagitannins, 2,3-(S)-HHDP-D-glucose and pedunculagin, showed more potent antioxidative activities by DPPH and TBARS than condensed tannins.

• 한국수산과학회지
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• 제20권5호
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• pp.441-446
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• 1987

가다랑어통조림 제조시 부산물로 얻어지는 가다랑어 자숙엑스분의 천연항산화성물질로서 효능을 검토하기 위하여 이의 지질산화억제효과 및 항산화성인자에 대하여 실험하였다. 가다랑어 자수엑스분의 아미노질소량은 15.3mg/100g 이었으며, methyl linoleate에 대해 뚜렷한 산화억제 작용을 나타내었고 가수분해 정도가 클수록 항산화력은 약간씩 저하하였다. 가다랑어엑스분을 증류수 중에서 투석시켰을때 투석내액은 상당한 항산화효과를 나타낸 반면, 투석내액은 거의 효과가 없었다. 가다랑어엑스분의 항산화성인자로서 anserine, carnosine, histidine 및 alanine 등가 같은 아미노산의 역할이 컸으며, AMP, hypoxanthine 등의 핵산관련물질도 항상화성에 보조적 역할을 하였다.

• 한국식품과학회지
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• 제23권5호
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• pp.578-581
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• 1991

EPA 및 DHA가 각간 25, 10% 함유된 어유에 상업용 레시틴을 첨가하여 어유의 산화안정성에 미치는 레시틴의 산화 방지효과를 AOM 시험, Oven 시험, 중합물 시험, 색도 시험 등을 통해서 비교하였다. 레시틴을 첨가한 산화안정성은 제품의 종류에 따라 산화안정성에 차이는 있었으나 첨가량이 증가할수록 산화안정성이 증가됨을 알 수 있었다. 가장 우수한 항산화성을 갖고 있었던 레시틴(III)을 1, 5, 10%을 첨가함에 따라 AOM 조건에서 유도시간이 각각 310, 620, 840%로 증가하였다. 또한 레시틴(III)을 10% 첨가한 시료구에서는 AOM 조건에서 10시간까지 중합물이 생성되지 않았으나 색도는 저장시간이 경과할수록 증가하였고 고온에서 더욱 가속화되었다.

• 대한본초학회지
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• 제23권2호
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• pp.151-157
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• 2008

Objectives : The purpose of this study is to investigate the biological Effect of multi-herbal drug 'KOCO-P1' on mouse macrophage Raw 264.7 cells. Methods : Multi-herbal drug 'KOCO-P1' was composed of Ginseng Radix, Astragali Radix, Polygonati Rhizoma, Liriopis Tuber, and Scrophulariae Radix. Cytotoxicity and cytoprotective activity of K0C0-P1 was verificated by MTT assay. And antioxidative effect of K0C0-P1 against EtOH, Nicotine was inspected by Hydroperoxide assay. Results : K0C0-P1 showed no cytotoxicity on RAW 264.7 cells for 24, 48, 72 hours. KOCO-P1 at 200, 100, and 50 ug/mL reduced the production of H202 in Raw 264.7 cells by EtOH. KOCO-P1 at 50 ug/mL reduced the production of H202 in Raw 264.7 cells by Nicotine. Conclusions : KOCO-P1 could be supposed to have antioxidative effect on macrophage with no cytotoxicity.

• 한국식품영양학회지
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• 제18권1호
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• pp.54-62
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• 2005

The antioxidative effect, antimutagenic capacity and inhibitory effect of cancer cell proliferation in ethanol extracts of Ganoderma lucidum were studied for suggestion of prevention and dietetic treatment of chronic diseases and development of antioxidative, antimutagenic and anticancer functional food by employing biological and biochemical assay. The IC/sub 50/ of MDA with BSA conjugation reaction, lipid peroxidation and scavenging effect on DPPH radical in ethanol extracts of Ganoderma lucidum showed 1026.21 mg/mL, 0.152 mg/mL and 0.412 mg/mL, respectively. So, the most effective antioxidative capacity in ethanol extracts of Ganoderma lucidum was the lipid peroxidation, among the method used this study. The indirect and direct antimutagenic effects of ethanol extracts of Ganoderma lucidum were examined by Ames test using Salmonella typimurium TA98 and TA100. The inhibition rates on indirect mutagenicity mediated by 2-Anthramine showed 100% in the Salmonella typimurium TA98 and 98.74% in the Salmonella typimurium TA100 and the direct mutagenicity mediated by sodium azide in Salmonella typimurium TA100 was 82.96%. But, the inhibitory effect on indirect mutagenicity mediated by 2-Nitrofluorene in Salmonella typimurium TA98 was low(7.81%). The inhibitory Effect of Ganoderma lucidum ethanol extracts on cell proliferation in Hela and MCF-7 by MTT test were 74.36% in HeLa and 73.90% in MCF-7 at the 0.50 mg/assay concentration and IC/sub 50/ were 0.163 mg/mL and 0.196 mg/mL respectively. From this result, it is suggested that Ganoderma lucidum is believed to have a possible antioxidative, antimutagenic and anticancer capacities.

• 대한임상검사과학회지
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• 제43권3호
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• pp.98-104
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• 2011

To evaluate the physioactivity of Salicornia herbacea L. (SH), which are obtained from Sunchon bay as wild plants, an SH extract was prepared by freeze drying to obtain SH, and by cold drying to obtain SH. For the evaluation of their bioactivities, cell viability and antioxidative effect were measured. The XTT assay was adopted to measure cell viability after C6 glioma cells were treated with various concentrations of reactive oxygen species (ROS) and hydrogen peroxide ($H_2O_2$) for 8 hours. The DPPH-radical scavenging activity was also measured for the antioxidative effect. In this study, the $XTT_{50}$ value of $H_2O_2$ was determined at $30{\mu}M$ which was highly toxic based on the cytotoxic criteria by Borenfreund and Puerner. The protective effect of SH extract significantly increased cell viability compared with $H_2O_2$-treated group. Its antioxidative effect showed a significant DPPH-radical scavenging activity at concentrations of $1-100{\mu}g/mL$, while SH extract showed highly a DPPH-radical scavenging activity at only $100{\mu}g/mL$. From these results, $H_2O_2$ was highly toxic in cultured C6 glioma cells, and SH extract was effective in the prevention of cell damage by its antioxidative effect.