• Journal of the Korean Chemical Society
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• v.41 no.10
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• pp.519-523
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• 1997

• Journal of the Korean Magnetic Resonance Society
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• v.8 no.2
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• pp.96-107
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• 2004

We prepared two oligonucleotides containing same base pairing, but different base sequence in the middle region, d(CGAATTCG) and d(CGTATACG). NMR and UV absorbance data represented that such variation in base sequence could cause a significant difference in melting temperature and dynamics between d(CGAATTCG)$_2$ and d(CGTATACG)$_2$ duplexes, which are regarded to be associated with the stacked structure and the width of the minor groove of them. The latter showed poor stability compared to the former, because of poor stacking of bases. And berenil could bind to the minor groove of d(CGAATTCG)$_2$ which is relatively narrow, more strongly than d(CGTATACG)$_2$ and this gave rise to large improvement in thermal stability of the d(CGAATTCG)$_2$ duplex, compared to d(CGTATACG)$_2$.

• Bulletin of the Korean Chemical Society
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• v.17 no.2
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• pp.109-112
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• 1996

• Bulletin of the Korean Chemical Society
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• v.16 no.8
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• pp.755-760
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• 1995

• Korean Journal of Veterinary Service
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• v.18 no.3
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• pp.42-52
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• 1995

Anaplasmosis is most important tick-borne rickettsial diseases of domestic ruminant. with Anaptasma spp. as their respective causal agents. In order to the survey prevalence of anaplasmosis in Korean native goats, we examined the Giemsa's blood filrms from 552 grazing and 188 nongrazing-goats in Chonbuk area Namwon, Imsil and Sunchang. The conclusive diagnosis was made by observing the characteristic marginal or central bodies in the red blood cells. The infection rate of grazine and nongrazing-goats with Anaplasma spp. was 71.7% and 8.5%, respectively. In order to study about the therapeutic effects of drug for 40 grazing-oats infected with Anaptasma spp. in mountain-area, Berenil(diminazene acetate) was jntramuscularly injected (0.5㎖ /10kg B.W.). After 1 month, we don't detected the parasite in the blood films from 33 goats(82.5%), but no change of significant hematological values(PCV, ESR, WBC, RBC, MCH, MCHC, PLT, MPV, PDW and HB) was observed. These results indicated that the infection rate of Anaplasma spp. was higher on glazing than nongrazing-goats and Berenil as an antianaplasmal drug is recommended.

• Bulletin of the Korean Chemical Society
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• v.17 no.11
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• pp.1045-1052
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• 1996

The effects of G-T mismatches on thermal stability, the base-pair lifetime and the global structure of a d(GCGTGCGC)2 duplex were studied by using 1H NMR, UV and CD spectroscopy. The existence of G-T mismatches was found to cause a noticeable change in the chemical environment of imino protons associated with significant decrease in the base-pair lifetime at the mismatched site as well as in thermal stability of the duplex itself. The melting transition of d(GCGTGCGC)2 was not cooperative at all at 100 mM or lower concentration of NaCl, but became cooperative at 500 mM or higher NaCl concentration. The melting temperature (Tm) of this duplex was 32℃ at 500 mM concentration of NaCl, which is much lower than that of d(GCGCGCGC)2 at the same NaCl concentration. This suggests that the decrease in stability may be ascribed to the decrease in the base-pair lifetime and the deviation from the normal structure due to the G-T mismatches. Adding berenil to d(GCGTGCGC)2 caused no observable change in the global structure but the large decrease in the base-pair lifetime and the stability of the duplex.

• Korean Journal of Veterinary Research
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• v.27 no.2
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• pp.321-330
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• 1987

In the endemic area of bovine piroplasmosis in Chonbuk Province, total of 486 heads of Holstein cow selected from 14 farms randomly were examined the infection status of piroplasma and their blood values. The results obtained were summarized as follows: 1. The etiological agent was only Theileria sergenti and none of the Babesia sp. infection. 2. All of the 486 heads of examined cow were infected with Theileria sergenti (100%). 3. In the initial examination of 11 farms in early summer, the means of blood values were as follows; RBC:$526{\pm}84(10^4/mm^3$), Ht:$27.9{\pm}2.8%$, SP:$7.5{\pm}0.6g/dl$, WBC: $11586{\pm}354/mm^3$, and Fibrinogen: $578.5{\pm}164.1mg/dl$. 4. In the second examinations in slimmer season, the blood values of examined cows had little varieties. But in the winter season, cows housed in the barn, the blood values were greatly increased; RBC: $601{\pm}77(10^4/mm^3)$, (F-value: 37.186**), Ht: $30.5{\pm}1.3%$(F-value: 15.626**) Hb: $12.2{\pm}1.3g/dl$(F-value: 5.899**), SP: $7.4{\pm}0.6g/dl$(F-value: 5.05**). 5. To determine the piroplasma infection in the many herds of cow, the measurement of Ht, Hb, and SP values etc. were more effective and helpful to find the weak cow because the examined process are not only to be easily carry out many samples but also to be done very simple and rapidly. 6. The indications of therapeutic standard values were stablished as follows; the less than 20% in the Ht value, below the 10g/dl in the Hb value, and less than 6g/dl in SP value. When the one among the therapeutic standard values, Ht, Hb, and SP was below the therapeutic standard value, the cow was treated with nutriments. When more than two among them were below the therapeutic standard, the cow was treated with Berenil, at once. In this way, there was no one head which represented clinical signs of piroplasmosis among 486 heads of cow at 14 farms during the year of 1986. 7. In a Korean native cattle infected with Thieleria sergenti seriously, the recovery of blood values was very prolonged by the treatment of Berenil only. But by the transfusion of 300ml blood collected from normal cow, the blood values were recovered rapidly.