• Title/Summary/Keyword: binding amounts of lectins

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Binding of $^3$H-Lectin from Kintoki Bean and Taro Tuber to Small Intestine of the Mouse (콩과 토란에서 추출한 $^3$H-Lectin의 마우스 소장에의 흡착량 정량)

  • 서영주
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.4
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    • pp.489-493
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    • 1993

  • The major objective of this study carried out was to compare the binding of Kintoki bean lectin (KBL) and Taro tuber lectin (TTL) to the mouse intestinal segments using $^3$H-labeled lectins and to assess the effect of such binding on the ability of the small intestine. Binding of $^3$H-KBL or $^3$H-TTL was studied under various conditions of time course, temperature, concentration, pH and additives of sugars, EDTA or unlabeled native lectin. The interaction of the lectins to intestinal tissue was stronger in KBL than in TTL, which was supposed to be the major reason for the stronger antinuritional enen of KBL. The optimal binding conditions were at 37$^{\circ}C$ for 60mins and at pH 7. The binding of both lectins were inhibited by fetuin and EDTA.

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