• 제목, 요약, 키워드: biofilm

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Inverse Correlation between Extracellular DNase Activity and Biofilm Formation among Chicken-Derived Campylobacter Strains

  • Jung, Gi Hoon;Lim, Eun Seob;Woo, Min-Ah;Lee, Joo Young;Kim, Joo-Sung;Paik, Hyun-Dong
    • Journal of Microbiology and Biotechnology
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    • v.27 no.11
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    • pp.1942-1951
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    • 2017
  • Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of C. jejuni NCTC11168. In addition, the strains degraded pre-formed biofilms of C. jejuni NCTC11168. Degradation of C. jejuni NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains C. jejuni Y23-5 and C. coli 34-3 isolated from raw chickens and the C. jejuni NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including C. jejuni 2-1, C. coli 34-1, and C. jejuni 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, C. jejuni 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming C. jejuni or C. coli strains of chicken origin.

Biofilm Formation of Food-borne Pathogens under Stresses of Food Preservation (식품 보존 스트레스에서의 식중독세균의 생체막 생성)

  • Lee, No-A;Noh, Bong-Soo;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.38 no.1
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    • pp.135-139
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    • 2006
  • Most bacteria form biofilm as self-defence system, making efficient food sanitization, preservation, and instrument washing more difficult. Biofilm formation of Salmonella, E. coli, B. cereus, and S. aureus was observed during 24 hr food preservations by performing microtiter plate and glass wool assays. Most cells formed biofilm and attached onto glass wool. When biofilm formation and injury were analyzed on the microtiter plate, 10 and 20% acid-injured E. coli and S. aureus, respectively, 30-50% cold temperature $(4^{\circ}C)-injured$ B. cereus and E. coli, and 30-55% 6% sodium chloride solution-injured Salmonella showed significant biofilm formation. Results indicate biofilm formation level differed within species depending on type of stress.

Pseudomonas aeruginosa Biofilm, a Programmed Bacterial Life for Fitness

  • Lee, Keehoon;Yoon, Sang Sun
    • Journal of Microbiology and Biotechnology
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    • v.27 no.6
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    • pp.1053-1064
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    • 2017
  • A biofilm is a community of microbes that typically inhabit on surfaces and are encased in an extracellular matrix. Biofilms display very dissimilar characteristics to their planktonic counterparts. Biofilms are ubiquitous in the environment and influence our lives tremendously in both positive and negative ways. Pseudomonas aeruginosa is a bacterium known to produce robust biofilms. P. aeruginosa biofilms cause severe problems in immunocompromised patients, including those with cystic fibrosis or wound infection. Moreover, the unique biofilm properties further complicate the eradication of the biofilm infection, leading to the development of chronic infections. In this review, we discuss the history of biofilm research and general characteristics of bacterial biofilms. Then, distinct features pertaining to each stage of P. aeruginosa biofilm development are highlighted. Furthermore, infections caused by biofilms on their own or in association with other bacterial species (i.e., multispecies biofilms) are discussed in detail.

A Study on Biofilm Detachment in an IFBBR (역 유동층 생물막 반응기에서의 생물막 탈착에 관한 연구)

  • 김동석;박영식
    • Journal of Environmental Science International
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    • v.3 no.3
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    • pp.263-271
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    • 1994
  • A detachment of biofilm was investigated in an inverse fluidized bed biofilm reactor(IFRBR). The biofilm thickness, 5 and the bioparticle density, Pm were decreased by the increase of Reynolds number, Re and the decrease of biomass concentration, h. The correlations were expressed as $\delta$=6l.6+16.33$b_c$-0.004Re and Ppd=0.3+0.027$b_c$- 2.93x$l0^{-5}$ no by multiple linear regression analysis method. Specific substrate removal rate, q was derived by F/M ratio and biofilm thickness as q=0.44.+0.82F/M-5.Ix10$-4^{$\delta$}$. Specific biofilm detachment rate, bds was influenced by FIM ratio and Reynolds number as $b_{ds}$=-0.26+0.26F/M+ 2.17$\times$$10^{-4}$Re. Specific biofilm deachment rate in an IFBBR was higher than that in a FBRR(fluidized bed biofilm reactor) because of the friction between air bubble and the bioparticles.

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Physical Properties of Biofilm Manufactured from Gelatin of Yellowfin Tuna Thunnus albacares Skin Treated with Acetic Acid (아세트산 처리 황다랑어(Thunnus albacares) 껍질 유래 젤라틴으로 제조한 바이오필름의 물리적 특성)

  • Kim, Ju-Yeon;Kim, Do-Hyeong;Kim, Seon-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.591-596
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    • 2011
  • The present study examined the physical properties of biofilms manufactured from yellowfin tuna Thunnus albacares skin gelatin with the aim of developing a biofilm from fisheries by-products to replace mammalian sources. The physical properties of biofilms from yellowfin tuna gelatin were compared with those of biofilms from porcine gelatin. The yellowfin tuna gelatin biofilm exhibited higher tensile strength (69.08 MPa) and greater elongation (14.32%) than did porcine gelatin biofilm (50.50 MPa and 10.21%, respectively). The ${\Delta}E$ and YI (yellowness index) Huntercolor values of yellowfin tuna gelatin biofilm were three-fold and 15-fold higher, respectively, than values for porcine gelatin biofilm. The opacity value of yellowfin tuna gelatin biofilm was higher than that of porcine gelatin biofilm. The stability against water of yellowfin tuna gelatin biofilm was lower than that of porcine gelatin biofilm at pH 3 to pH 11. Thermogravimetric analysis (TGA) indicated that the thermal stability of the biofilms was about $270^{\circ}C$ for porcine gelatin biofilm and about $250^{\circ}C$ for yellowfin tuna gelatin biofilm.

Bioadhesive Characteristics of Biofilm Manufactured from Gelatin Derived from Acetic Acid-treated Skin of the Yellowfin Tuna Thunnus albacares (아세트산처리 황다랑어(Thunnus albacares) 껍질 유래 젤라틴으로 제조한 바이오필름의 생체 접착 특성)

  • Kim, Ju-Yeon;Kim, Do-Hyeong;Moon, Chang-Kwon;Kim, Seon-Bong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.44 no.6
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    • pp.584-590
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    • 2011
  • This study aimed to demonstrate the bioadhesive characteristics of gelatin biofilm to rat skin. The biofilm was manufactured from gelatin extracted from the acetic acid treated-skin of the yellowfin tuna Thunnus albacares. The bioadhesive strength of tuna gelatin biofilm was compared to that of porcine gelatin biofilm. The tuna gelatin biofilm exhibited a higher bioadhesive strength than the porcine gelatin biofilm. Gelatin biofilm was subjected to glutaraldehyde treatment at different concentrations, temperatures and pH in order to improve its bioadhesive strength. Glutaraldehyde treatment improved the bioadhesive strength of gelatin biofilm up to three-fold. The bioadhesive strength of glutaraldehyde treated-biofilm was significantly decreased by application of sodium borohydride.

Culture Condition for Listeria monocytogenes 1421 Biofilm Formation and the Effect of Kimchi on Biofilm (Biofilm 형성을 위한 Listeria monocytogenes 1421의 배양 조건과 김치에 의한 영향)

  • Kim, Eun-Ah;Mang, So-Yeon;Seong, Jong-Hwan;Lee, Young-Guen;Kim, Han-Soo;Kim, Dong-Seob
    • Journal of Life Science
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    • v.22 no.5
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    • pp.692-696
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    • 2012
  • $Listeria$ $monocytogenes$, a fatal food-borne pathogenic bacteria, can form a biofilm on many different supports. The biofilm gives $L.$ $monocytogenes$ more viability and resistance to disinfectants and sterilization procedures.$L.$ $monocytogenes$ formed biofilms on various culture vessels tested in this experiment and showed the maximum amount of biofilm when it was cultured for 4 days at $30^{\circ}C$ in BHI broth. In this study, biofilm formation was stimulated or inhibited by addition of different Kimchi samples. That was not in accordance with the effect of Kimchi on the growth of $L.$ $monocytogenes$.

Plant Extracts Inhibiting Biofilm Formation by Streptococcus mutans without Antibiotic Activity

  • Ham, Youngseok;Kim, Tae-Jong
    • Journal of the Korean Wood Science and Technology
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    • v.46 no.6
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    • pp.692-702
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    • 2018
  • Streptococcus mutans causes oral diseases, including tooth decay, by producing a biofilm called plaque. Therefore, inhibition of biofilm formation is essential for maintaining oral health. Plants produce a variety of secondary metabolites, which act as starting sources for the discovery of new bioactive chemicals that inhibit biofilm formation of S. mutans. Previous studies have reported on chemicals with antibiotic activity for the inhibition of biofilm formation by S. mutans. In this study, nine plant extracts from Melonis Pedicellus, Agastachis Herba, Mori Cortex Radicis, Diospyros kaki leaves, Agrimoniae Herba, Polygoni Multiflori Radix, Lycopi Herba, Elsholtziae Herba, and Schizonepetae Spica were screened for the inhibition of biofilm formation from a plant extract library. The water-soluble compounds of the extracts did not affect cell growth but selectively inhibited biofilm formation. These results suggest that the selected plant extracts constitute novel biofilm formation inhibitors, with a novel biological mechanism, for improving oral hygiene.

Isolation of bacteriophages having depolymerase and control of pathogenic E. coli O103 in biofilm on lettuce

  • Park, Dasom;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
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    • v.51 no.6
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    • pp.604-609
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    • 2019
  • To control pathogenic E. coli in biofilm, bacteriophages were isolated from environmental samples. Seventeen isolates had depolymerase activities by translucent zones at the rims of plaques. To determine biofilm-forming ability, an abiotic plastic surface of polystyrene was used; E. coli O103 showed the highest biofilm formation at 30℃ after 24 h. Moreover, biofilm by E. coli O103 on the biotic surface of lettuce was observed by a scanning electron microscope. The bacteriophage cocktail of ΦNOECP40 and ΦNOECP44 showing depolymerase activities was prepared to eliminate the E. coli inbiofilm. By organic acids, reduction of E. coli in biofilm was insignificant and almost undetectable. However, the abundance of E. coli in biofilm was reduced by 3 log CFU/mL from 7.3 log CFU/mL after 60 min with the bacteriophage cocktail. Therefore, we suggest that bacteriophages with depolymerase could be utilized to effectively control pathogenic E. coli in biofilm.

Time-course Analysis of Biofilm Formation in Quorum Sensing-deficient Bacteria (Quorum sensing 결핍 세균에서 생물막 형성의 시간적 추이 분석)

  • Kim, Soo-Kyoung;Lee, Mi-Nan;Lee, Joon-Hee
    • Korean Journal of Microbiology
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    • v.50 no.2
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    • pp.108-113
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    • 2014
  • Pseudomonas aeruginosa and Vibrio vulnificus are Gram-negative human pathogens, which exert their virulence through quorum sensing (QS) regulation. The infection of these pathogens have been known to be mediated by biofilm formation in many cases and this study carried out the time-course analysis of biofilm formation depending on the QS regulation in P. aeruginosa and V. vulnificus. In P. aeruginosa, our results demonstrated that QS-deficient mutant better attached to surface at initial stage of biofilm formation, but poorly proceeded to the maturation of the biofilm structure, while wild type less attached at initial stage but developed highly structured biofilm at late stage. Because of this, the quantitative comparison of biofilm formation between wild type and the QS mutant showed the reversion; the QS mutant formed more biofilm until 10 h after inoculation than wild type, but wild type formed much more biofilm after 10 h than QS mutant. V. vulnificus has been reported to form more biofilm with the mutation on QS system. When we performed the same time-course analysis of the V. vulnificus biofilm formation, the reversion was not detected even with prolonged culture for 108 h and the QS mutant always forms more biofilm than wild type. These results indicate that the QS regulation negatively affects the attachment at early stage but positively facilitates the biofilm maturation at late stage in P. aeruginosa, while the QS regulation has a negative effect on the biofilm formation throughout the biofilm development in V. vulnificus. Based on our results, we suggest that the developmental stage of biofilm and bacterial species should be considered when the QS system is targeted for biofilm control.