• Title, Summary, Keyword: biological activity

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Antioxidant and Antimicrobial Activity of Zostera marina L. Extract

  • Choi, Han-Gil;Lee, Ji-Hee;Park, Hyang-Ha;Sayegh, Fotoon A.Q.
    • ALGAE
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    • v.24 no.3
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    • pp.179-184
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    • 2009
  • Methanol crude extract of the sea grass Zostera marina L. and organic solvent fractions (n-hexane, chloroform, ethyl acetate, n-butanol, and water) were screened for antioxidant activity (total phenolic contents, DPPH scavenging activity, and reducing power) and antimicrobial activity against three human skin pathogens, two bacteria and a yeast; Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans. Total phenolic contents and 2, 2- diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were highest in the ethyl acetate fraction with 968.50 $\mu$g gallic acid equivalent per milligram of extract, and ca. 95% scavenging activity on the DPPH radicals at 10 mg $ml^{-1}$. In antimicrobial activity tests, MICs (Minimum Inhibitory Concentration) of each Zostera marina extract partitioned ranged from 1mg to 8 mg $ml^{-1}$ (extract/ 10% DMSO) against all three human skin pathogens. The MICs of the ethyl acetate and n-butanol fractions were the same with 1 mg $ml^{-1}$ against S. aureus and C. albicans. The ethyl acetate fraction of Z. marina does protect against free radicals and may be used to inhibit the growth of human skin pathogens.

A Novel Thrombolytic and Anticoagulant Serine Protease from Polychaeta, Diopatra sugokai

  • Kim, Hye Jin;Shim, Kyou Hee;Yeon, Seung Ju;Shin, Hwa Sung
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.275-283
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    • 2018
  • Ischemic stroke can result from blockage of blood vessels, forming fibrin clots in the body and causing irreparable brain damage. Remedial thrombolytic agents or anticoagulants have been studied; however, because the FDA-approved tissue plasminogen activator has low efficacy and side effects, it is necessary to develop safer and more effective treatment candidates. This study aimed at assessing the fibrinolytic and anticoagulation features of a novel serine protease extracted and purified from Diopatra sugokai, a polychaeta that inhabits tidal flats. The purified serine protease was obtained through ammonium sulfate precipitation, affinity chromatography, and ion-exchange chromatography. Its molecular size was identified via SDS-PAGE. To characterize its enzymatic activities, the protease activity at various pH and temperatures, and in the presence of various inhibitors, was measured via azocasein assay. Its fibrinolytic activity and anticoagulant effect were assessed by fibrin zymography, fibrin plate assay, and fibrinogenolytic activity assays. The novel 38 kDa serine protease had strong indirect thrombolytic activity rather than direct activity over broad pH (4-10) and temperature ($37^{\circ}C-70^{\circ}C$) ranges. In addition, the novel serine protease exhibited anticoagulant activity by degrading the ${\alpha}$-, ${\beta}$-, and ${\gamma}$-chains of fibrinogen. In addition, it did not produce cytotoxicity in endothelial cells. Therefore, this newly isolated serine protease is worthy of further investigation as a novel alkaline serine protease for thrombolytic therapy against brain ischemia.

Synthesis and Biological Activity of Aspirin Derivatives

  • Cha, Bae-Cheon;Lee, Seung-Bae
    • Archives of Pharmacal Research
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    • v.23 no.2
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    • pp.116-120
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    • 2000
  • Aspirin has been widely used as analgesic and anti-inflammatory drug. Recently, it was elucidated that aspirin have anti-coaggregatory effect in low dose. This study was carried out to investigate the synthesis of aspirin derivatives from aspirin and aromatic compound of antioxidant and its biological activities. Synthesis of aspirin derivatives was prepared by esterification in the presence of 1, 1-carbonyldiimidazole. Biological activities was examined using effect of anti-coagulant on bleeding time, effect of antioxidant and effect of anti-platelet aggregation. As a result, SJ-101 showed strong antioxidative activity and anti-coagulant activity among four compounds. Anti-platelet aggregation of SJ-101 was examined by collagen, ADP, PAF method. SJ-101 exhibited more stronger activity to aspirin at collagen aggregation reaction. These finding demonstrates that SJ-101 is usefull as care drug of aging and old-disease because of its has antioxidant activity, anti-coagulant activity and anti-platelet activity.

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Evaluation of the Biological Activity of Extracts from Star-Anise (Illicium verum)

  • Chung, Hai-Jung
    • Preventive Nutrition and Food Science
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    • v.14 no.3
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    • pp.195-200
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    • 2009
  • This study was carried out to investigate the biological activities of star anise extracts obtained from four different solvents (water, methanol, ethanol and chloroform) by measuring total polyphenol and flavonoid contents, DPPH radical scavenging, hydroxyl radical scavenging, nitrite scavenging activity and antimicrobial activity. The methanol extract showed the highest extraction yield, followed by ethanol, water and chloroform. The properties of the extracting solvents significantly affected the total polyphenol content. Methanol extracts contained more total polyphenols than any other extracts. The highest DPPH radical scavenging activity was found in methanol extract. The hydroxyl radical and nitrite scavenging activity were highest in methanol and ethanol extracts. In antimicrobial activity, water extract showed stronger activities than methanol and ethanol extract against Micrococcus luteus and Bacillus subtilis, and no inhibitory effects on Gram negative bacteria were found in all extracts at the concentration used.

Immobilization of Trypsin onto Silk Fibroin Fiber via Spacer Arms

  • Lee, Ki-Hoon;Kang, Gyung-Don;Shin, Bong-Seob;Park, Young-Hwan;Nahm, Joong-Hee
    • International Journal of Industrial Entomology
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    • v.8 no.2
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    • pp.195-200
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    • 2004
  • Trypsin can be immobilized on silk fibroin fiber (SFF) by introducing several spacer arms, such as ethylene diamine (ED), bovine serum albumin (BSA) and silk sericin (SS). Direct immobilization on silk fiber (SFFGA) has low activity because of the steric hindrance between the trypsin and substrate. The introduction of spacer arms onto SFF-GA can enhance the activity of trypsin by reducing the steric hindrance. When ED is used as a spacer arm, the activity of trypsin has increased but its stability decreased due to the increased hydrophobicity of SFF. BSA and SS, as a spacer arm, have better results in both activity and stability. SFF-BSA shows some decrease in the specific activity due to improper immobilizatin. SFF-SS maintained 90% of its initial activity even after 12 hrs incubation at $50^{\circ}C$. In the case of repeated hydrolysis of silk sericin with immobilized trypsin, SFF-GA and SFF-ED lost 50% of their initial activity right after first run, whereas SFF-BSA and SFF-SS maintained 80% of their initial activities even after 5 runs. Higher operational stability is due to increased hydrophilicity of SFF by introducing hydrophilic spacer arms such as BSA and SS. The high content of serine in SS increases the hydrophilicity of SFF resulting the best results among other spacer arms.

Development of New Agrochemicals by Quantitative Structure-Activity Relationship (QSAR) Methodology -IV. A Tendency of Research and Prospect in Korea- (정량적인 구조-활성상관(QSAR) 기법에 의한 새로운 농약의 개발 -IV. 국내의 연구 동향과 전망-)

  • Sung, Nack-Do
    • Applied Biological Chemistry
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    • v.46 no.3
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    • pp.155-164
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    • 2003
  • It was reviewed for the status of domestic research before and after 1990's for search of a new pesticides using 2D QSAR of quantitative structure-activity relationship (QSAR) methodologies (Sung, Nack-Do (2002) Development of new agrochemicals by quantitative structure-activity relationship (QSAR) methodology. Kor J. Pestic. Sci. 6, 166-174, 231-243 & 7, 1-11) which was proposed according to Hansch-Fujita equation based on the concept of biological Hammett equation.

In vivo Antifungal Activity Against Various Plant Pathogenic Fungi of Curcuminoids Isolated from the Rhizomes of Curcuma longa

  • Cho, Jun-Young;Choi, Gyung-Ja;Lee, Seon-Woo;Lim, He-Kyoung;Jang, Kyung-Soo;Lim, Chi-Hwan;Cho, Kwang-Yun;Kim, Jin-Cheol
    • The Plant Pathology Journal
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    • v.22 no.1
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    • pp.94-96
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    • 2006
  • In a search for plant extracts with potent in vivo antifungal activity against various plant pathogenic fungi, the methanol extract of the Curcuma longa rhizomes effectively controlled the development of rice blast catised by Magnaporthe grisea and tomato late blight caused by Phytophthora infestans. Three curcuminoids such as curcumin, demethoxycurcumin, and bisdemethoxycurcumin were purified from the methanol extract of C. longa rhizomes as antifungal principles. Among the three curcuminoids, demethoxycurcumin was the most active to both rice blast and tomato late blight, followed in order by curcumin and bisdemethoxycurcumin. However, they all exhibited no or little in vivo antifungal activity against other fungal pathogens causing rice sheath blight (Corticium sasaki), tomato gray mold (Botrytis cinerea), wheat leaf rust (Puccinia recondita), or barley powdery mildew (Blumeria graminis f. sp. hordel).

Effect of Low Intensity Pulsed Electric Field on Ethanol Fermentation and Chemical Component Variation in a Winemaking Culture

  • Min, He-Ryeon;Jeon, Bo-Young;Seo, Ha-Na;Kim, Min-Ju;Kim, Jun-Cheol;Kim, Joon-Kuk;Park, Doo-Hyun
    • Food Science and Biotechnology
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    • v.18 no.6
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    • pp.1358-1364
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    • 2009
  • Electric polarity of working electrode and counter electrode was periodically switched at the intervals of 30 sec. Electric current generated by anodic and cathodic reaction of working electrode was reached to +30 and -12 mA in low intensity pulsed electric field (LIPEF). The yeast growth, ethanol production, and malate consumption in the initial cultivation time were more activated in the LIPEF than the conventional condition (CC). Polyphenol, total phenolic contents (TPC), and total flavonols (TF) were gradually decreased in all cultivation conditions during incubation for 2 weeks but antioxidation activity was not. TF was significantly lower in 3 and 4 V of LIPEF than CC and 2 V of LIPEF; however, the polyphenol, TPC, and antioxidation activity were a little influenced by the LIPEF. After ripening of the winemaking culture for 15 days, polyphenol, TPC, and TF were a little increased but the antioxidation activity was not.

In vivo control of phytopathogens by using omega-3 fatty acid docosahexaenoic acid (DHA) bioconverted by Pseudomonas aeruginosa PR3

  • Kang, Sun-Chul;Kim, Hak-Ryul;Shin, Seung-Yong;Bajpai, Vivek K.
    • 한국생물공학회:학술대회논문집
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    • pp.497-499
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    • 2005
  • Bioconverted hydroxy fatty acid, docosahexaenoic (bDHA) obtained from the microbial conversion by Pseudomonas aeruginosa PR3 was evaluated for its in vivo anti-fungal activity. bDHA showed great potential of anti-fungal activity against phytopathogenic fungi tested in this study. bDHA at the concentration of 500 ${\mu}g/ml.$ showed remarkable anti-fungal activity against all the fungus tested.

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None of The Four Tyrosine Residues is Essential for the Bio-logical Activity of Erythropoietin

  • Son, Homo;Lee, Jin-Hyung;Chung, Taeowan
    • Archives of Pharmacal Research
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    • v.18 no.6
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    • pp.371-375
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    • 1995
  • Erythropoietin (EPO), a glycoprotein hormone, regulates the proliferation and differentiation of ereythroid progenitor cells. Many attempts have been made to identify the functionally important amino acids of the hormone. One of those early studies has found that heavy redioiodination of EPO caused the loss of its biological activity, suggesting some important role of one of the four tyrosine residues (Goldwasser, 1981). Thus, in this study, we have generated and tested four $Tyr{\dashrightarrow}Phe$ substitution mutants to clarify the possible role of the tyrosine residue(s) in the hormone's Tyrosine residue(s) in the hormone's biological activity. When the mutant and wild type EPO cDBAs were transfected into COS-7 cells and the biological activities of the muteins were assayed using the primary murine erythroid spleen cells, no mutation tested was found to affect the biological activity of the hormone. Thus we conclude that, contrary to the previous observation, none of the four tyrosine in eryghropoietin is critically involved in the binding of the hormone to its receptor.

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