• 제목, 요약, 키워드: biological activity

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Biological Activity and Acute Toxicity of the Multimers of CJ500011 Recombinant Human Granulocyte Colony-stimulating Factor (rHuG-CSF), Produced in E. coli (재조합 사람 과립구 콜로니 자극인자인 C,J50001의 중합체의 생물학적 활성과 급성독성에 관한 연구)

  • 하석훈;이현수;김기완;정종상;김달현
    • Biomolecules & Therapeutics
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    • v.6 no.1
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    • pp.89-94
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    • 1998
  • CJ50001 is a recombinant human granulocyte colony-stimulating facto, (rHuG-CSF) that stimulates the formation of neutrophils from bone marrow stem cells. It was produced in E. colt and purified through refolding and several processes. We produced CS970125(300) using purified C150001 and additives in order to test the stability of CJ50001. When CS970125(300) was stored at 50'S for more than 1 week, high molecular weight proteins were formed and those proteins were detected by non-reducing SDS-PAGE, gel filtration HPLC, and Western blot. Those proteins showed single band at the same position of CJ50001 in reducing SDS-PAGE. These data indicated that those high molecular weight proteins were the multimers of C150001. In biological assays, iu viro and in viro, the multimers did not have biological activity and inhibitory action to that of CJ 50001. The mutimers did not induce toxicity in mice and rats in acute toxicity test. These results suggest that if Cs970125(300) containing CJ50001 is stored at 5$0^{\circ}C$, CJ50001 will be the multimers that do not have biological activity and inhibitory effect to CJ50001 and do not induce acute toxicity.

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Anti-septic activity of α-cubebenoate isolated from Schisandra chinensis

  • Kook, Minsoo;Lee, Sung Kyun;Kim, Sang Doo;Lee, Ha Young;Hwang, Jae Sam;Choi, Young Whan;Bae, Yoe-Sik
    • BMB Reports
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    • v.48 no.6
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    • pp.336-341
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    • 2015
  • Sepsis is a life-threatening, infectious, systemic inflammatory disease. In this study, we investigated the therapeutic effect of α-cubebenoate, a novel compound isolated from Schisandra chinensis against polymicrobial sepsis in a cecal ligation and puncture (CLP) experimental model. Administration of α-cubebenoate strongly enhanced survival in the CLP model. α-cubebenoate administration also markedly blocked CLP-induced lung inflammation and increased bactericidal activity by enhancing phagocytic activity and hydrogen peroxide generation in mouse bone marrow-derived macrophages and neutrophils. Expression of two important inflammatory cytokines, IL-1 and IL-6, was strongly increased in the CLP model, and this was dramatically blocked by α-cubebenoate. Lymphocyte apoptosis and caspase-3 activation, which are associated with immune paralysis during sepsis, were markedly attenuated by α-cubebenoate. Taken together, our findings indicate that α-cubebenoate, a natural compound isolated from Schisandra chinensis, is a powerful potential anti-septic agent. [BMB Reports 2015; 48(6): 336-341]

Enhancement of Biocontrol Activity of Antagonistic Chryseobacterium Strain KJ1R5 by Adding Carbon Sources against Phytophthora capsici

  • Kim, Yu-Seok;Jang, Bo-Ra;Chung, Ill-Min;Sang, Mee-Kyung;Ku, Han-Mo;Kim, Ki-Deok;Chun, Se-Chul
    • The Plant Pathology Journal
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    • v.24 no.2
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    • pp.164-170
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    • 2008
  • Carbon utilization by Chryseobacterium strain KJ1R5 was studied to enhance its biocontrol activity against Phytophthora capsid. Chryseobacterium strain KJ1R5 has previously been shown to control Phytophthora blight of pepper (Capsicum annuum L.). Strain KJ1R5 could utilize carbon sources such as L-arabinose, D-cellobiose, ${\beta}-lactose$ and D-galactose well. P. capsici could utilize D-glucose well, showing the absorbencies ranged from 0.577 to 0.767 at 600nm. When 2% L-arabinose, which could only be utilized by the bio-control strain KJ1R5, was amended into the bacterial suspension, the efficacy of biological control increased. Among the amendments of various carbon sources into bacterial suspension, L-arabinose and D-(+)-glucose significantly enhanced biological control activity, resulting in a reduction of disease incidence to 6.9%, compared to 21.9% for the strain KJ1R5 alone and 81.3% for P. capsici inoculation alone, indicating that amendment with specific carbon sources could increase the biological control activity.

Antioxidant Activity and Biological Activity of Specialty Barley (Hordeum vulgare L.) Extracts

  • Lee, Na-Young;Kim, Yang-Kil;Choi, In-Duck;Cho, Sang-Kyun;Kweon, Hun-Jung;Lee, Kwang-Won;Hyun, Jong-Nae;Choi, Jae-Seong;Park, Ki-Hun;Kim, Kee-Jong;Lee, Mi-Ja
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.55 no.2
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    • pp.159-164
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    • 2010
  • Specialty barley extracts were prepared and investigated for its antioxidant activity and biological activity. Hunter $L^*$ values of the Iksan 86 extracts had higher than that of the Iksan 87 and Zasoojeongchal extracts. The extraction yields of Iksan 86, Iksan 87, and Zasoojeongchal was 8.08, 6.62, and 7.30%, respectively. The contents of total phenolic compounds of the Iksan 86, Iksan 87, and Zasoojeongchal extracts were 16.24, 15.51, and 13.95 GAE mg/g of sample, respectively. The DPPH radical scavenging activity of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 50.00, 33.27, and 7.56% at a 500 ppm, respectively. The samples showed an inhibition of xanthin oxidase. ACE inhibition effect of specialty barley extracts, Iksan 86, Iksan 87, and Zasoojeongchal, was 39.81, 41.06, and 27.78%, respectively. Tyrosinase inhibition rates (%) of Iksan 86, Iksan 87, and Zasoojeongchal extracts were 26.21, 24.57, and 20.00%, respectively. Results indicated that specialty barley extracts possesses various biological activities including antioxidative capacity, xanthin oxidase inhibition activity, angiotensin converting enzyme inhibition activity, and tyrosinase inhibition activity.

Antioxidant Activity of Hawthorn Fruit in vitro

  • Li, Chunmei;Han, Woong;Wang, Myeong-Hyeon
    • Journal of Applied Biological Chemistry
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    • v.53 no.1
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    • pp.8-12
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    • 2010
  • The antioxidant activity of hawthorn fruit (Crataegus pinnatifida Bunge var. typica Schneider) extracts was investigated by several in vitro antioxidants properties, including DPPH free radical scavenging activity, total phenolic and flavonoid contents, hydroxyl radical scavenging activity, reducing power activity, iron-chelating capacity and nitrite scavenging activity. Among the extracts in this study, the 70% EtOH extract showed higher antioxidant activity than the others. The $IC_{50}$ value of DPPH free radical scavenging activity was $99.26\;{\mu}g/mL$. Furthermore, the 70% EtOH extract also showed significantly high total phenolic and flavonoids contents and reducing power activity. However, the MeOH extract exhibited stronger effects on hydroxyl radical scavenging activity, iron-chelating capacity and nitrite scavenging activity. All the results implicated that, the hawthorn fruit may has the available potential to be utilize as a potential source of natural antioxidant.

Research on antifungal and insecticidal activity of Natural extracts for Protecting Organic Cultural heritages (유기질문화재 보존을 위한 천연 추출물의 항균·살충활성 연구)

  • Choi, Jung-Eun;Kim, Young-Hee;Hong, Jin-Young;Jung, Mi-Hwa;Jo, Chang-Wook
    • 보존과학연구
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    • pp.89-102
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    • 2010
  • This study evaluated the activity of biocidal compounds isolated from natural products and confirmed applicable possibility as conservative agents for orgainic cultural heritages damaged by biological degradation. we used methanol extracts of 11 Korean trees and 17 medicinal plants as natural resources. Among these extracts, Phellodendron amurense was investigated on antifungal activity against Coniophora puteana, Lentinus lepideus, Tyromyces palustris and Aspergillus niger. Asarum sieboldii was investigated on insecticidal activity against adult of Lasioderma serricorne. First, the extract of Phellodendron amurense was partitioned into hexane, dichloromethan and ethylacetate fraction. Among them, the ethylacetate fraction had the most significant antifungal activity. In addition, Asarum sieboldii essential oil showed an effective antifungal and insecticidal activity. As a result, the extract of Phellodendron amurense and Asarum sieboldii essential oil could be useful for conservation of organic cultural heritage against biological deterioration by wood rot fungi and Lasioderma serricorne.

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Synthesis of Pyrimidine Nucleoside Analogues and Screening of Their Biological Effects (Pyrimidine Nucleoside 유도체들의 합성 및 약물학적 효능 검색)

  • 신혜순;이희주
    • Biomolecules & Therapeutics
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    • v.3 no.3
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    • pp.217-222
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    • 1995
  • Several acyclonucleoside analogues of pyrimidine base and N$^1$-derivatives of 5-fluorouracil have been synthesized and evaluated for their biological effects. When tested with in vitro Lekemia L1210 cells, the 5-fluorouracil derivatives exhibited slightly higher antitumor activity than the parent 5-fluorouracil. When tested against Herpes Simplex Virus type 1 and type 2 cultured in the Vero cell, the 5-fluorouracil derivatives showed weak antiviral activity.

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Antimicrobial Activity of Rumex crispus Fractions

  • Lee, Kyoung-Min;Jeong, Gwi-Taek;Park, Don-Hee
    • 한국생물공학회:학술대회논문집
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    • pp.268-270
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    • 2005
  • This study was carried out to investigate the antimicrobial activity of Rumex crispus development of antibiotics derived from natural products. To confirm antimicrobial activity, paper disc method and growth inhibition in liquid culture were applied. Antimicrobial activity was observed in Saccharomyces cerevisiae, Vibrio vulificus, Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Cαndida bombicola.

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Probing the Critical Residues for Intramolecular Fructosyl Transfer Reaction of a Levan Fructotransferase

  • Moon, Keum-Ok;Choi, Kyoung-Hwa;Kang, Ho-Young;Oh, Jeong-Il;Jang, Se-Bok;Park, Cheon-Seok;Lee, Jong-Hoon;Cha, Jae-Ho
    • Journal of Microbiology and Biotechnology
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    • v.18 no.6
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    • pp.1064-1069
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    • 2008
  • Levan fructotransferase (LFTase) preferentially catalyzes the transfructosylation reaction in addition to levan hydrolysis, whereas other levan-degrading enzymes hydrolyze levan into a levan-oligosaccharide and fructose. Based on sequence comparisons and enzymatic properties, the fructosyl transfer activity of LFTase is proposed to have evolved from levanase. In order to probe the residues that are critical to the intramolecular fructosyl transfer reaction of the Microbacterium sp. AL-210 LFTase, an error-prone PCR mutagenesis process was carried out, and the mutants that led to a shift in activity from transfructosylation towards hydrolysis of levan were screened by the DNS method. After two rounds of mutagenesis, TLC and HPLC analyses of the reaction products by the selected mutants revealed two major products; one is a di-D-fructose-2,6':6,2'-dianhydride (DFAIV) and the other is a levanbiose. The newly detected levanbiose corresponds to the reaction product from LFTase lacking transferring activity. Two mutants (2-F8 and 2-G9) showed a high yield of levanbiose (38-40%) compared with the wild-type enzyme, and thus behaved as levanases. Sequence analysis of the individual mutants responsible for the enhanced hydrolytic activity indicated that Asn-85 was highly involved in the transfructosylation activity of LFTase.

Plant Growth-promoting Activity of Acremonium strictum MJN1 Isolated from Roots of Panax ginseng

  • Lim, Hyung-Bum;Chung, Yang-Jo;Bae, Ju-Yun;Kim, Dong-Jin;Kwon, Hyung-Jin;Lee, In Hyung;Chung, Byung-Chul;Lee, Woong-Sang;Suh, Joo-Won
    • Journal of Applied Biological Chemistry
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    • v.43 no.2
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    • pp.104-108
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    • 2000
  • The plant growth-promoting activity of Acremonium strictum MJN1 isolated from roots of Panox ginseng was explored. The myceliaI extract of A. strictum MJN1 enhanced the rice seedling growth by 14.5 and 9.0% in the dried weight of shoots and roots, and the growth of red pepper by 54 and 85% in the top length and the dried weight in pot experiments, respectively. The plant growth-promoting substances in the myceliaI extract of Acremonium strictum MJN1 were identified as D-adenosine and glycerol. Both commercial D-adenosine and glycerol also promoted significantly the rice seedling growth but, unlike the mycelial extract of A. strictum MJN1, hardly affected the yields of plants grown in pots or field. Therefore, it is possible that other plant growth-promoting substances are produced by A. strictum MJN1. However, this study shows that A. strictum MJN1 has a great potential as a biofertilizer.

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