• Korean Journal of Veterinary Service
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• v.42 no.4
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• pp.265-274
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• 2019

Delayed arrival of blood samples from the field and a large number of samples delivered often causes delay in sample analysis leading to inaccurate measurements. Therefore, this study aimed to assess whether prolonged storage in refrigerator could influence the stability of cattle blood samples and to establish an optimal time limit for complete blood count (CBC) parameters and blood gas and electrolyte (BGE) parameters analyses. Samples collected from healthy cows were tested immediately for CBC and BGE using automated hematology, blood gas and electrolyte analyzers. Samples were kept in refrigerator at 4℃ and analyzed after 6 h, 12 h, 24 h, 48 h, 72 h, 120 h, and 192 h of storage. Mean differences between observations were assessed at 5% significance level using ANOVA and Duncan's multiple range test. Total CBC parameters and the platelet profile remained stable for 192 h, except for MCHC. Among leukocyte-related counts, NEU and EOS remained stable for 192 hours. WBC and LYM, and MONO values produced inconsistent measurements which recovered its initial measurement after 12 h and 24 h of storage, respectively, then remained stable until 120 h. Among the blood gas indices, PCO₂, PO₂, tCO₂, and BE showed declining and significant changes over time, but pH, tHb, and SO₂ remained stable for 192 h. Electrolyte status in the blood showed that ions are unstable and tend to change in as early as 6 h of storage. This study established that cattle blood specimens for CBC analysis can be stored for 120 h at 4℃, but specimens for BGE analyses must be tested within 6 to 24 h.

• Nutritional Sciences
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• v.8 no.1
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• pp.35-41
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• 2005

Controlling dietary behaviors and health-related lifestyle habits is important to manage non-insulin dependent diabetes mellitus (NIDDM). If NIDDM is not treated properly, the prevalence of macro-vascular complications (MC) may increase. The goal of NIDDM therapy is to maintain normal concentrations of blood glucose and lipid profiles by having regular meals, controlling alcohol drinking, quitting smoking, and performing physical exercise regularly. This study was performed to investigate the dietary and health-related lifestyle habits and blood parameters of NIDDM patients of both genders and compared these characteristics between the patients with and without MC. Our results show that the subjects with MC compared to without MC and the female patients than the males controlled their diets more regularly, tended to regulate the number of meals better, and smoke less than those without MC. Although plasma lipid profiles were not significantly different between the genders and between the subjects with and without MC, the men had higher blood glucose and plasma tHcy concentrations than the women and plasma tHcy concentration was higher in the female subjects without MC. These results imply that the male patients of NIDDM without MC might have more problems in maintaining their blood glucose. In addition, smoking may be the most important life-style factor influencing some blood parameters like blood glucose, HbAlc, and total cholesterol in the NIDDM patients.

• Journal of the Korean Dietetic Association
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• v.8 no.4
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• pp.333-341
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• 2002

Obesity, a state of having excessive body fat enough to attack one's health, is most blamed for causing chronical degenerative illness such as hypertension, diabetes mellitus as well as arteriosclerosis. According to many studies, the program for the weight control was the most demanded at the worksites. This study was designed to investigate the correlation between the degree of obesity and blood parameters of the employees at the worksites. The results of periodic medical examination for 571 employees(male: 418, female: 153) were analyzed; Age, BMI, systolic blood pressure(SBP), diastolic blood pressure(DBP), hemoglobin(Hb), fasting blood sugar(FBS), total-cholesterol(TC), serum glutamic oxaloacetic transaminase (sGOT), serum glutamic pyruvic transaminase(sGPT) and $gamma$-glutamyl transpeptidase($gamma$-GTP). Pearson correlation coefficient was used to find out the correlation between BMI and each blood parameters. In the case of male, 27.3% of the subjects were over-weight. The subject's age and their blood levels of uric acid, FBS, sGOT and sGPT did not show any significant difference regardless of BMI's, while SBP, DBP, Hb, TC and $gamma$-GTP levels of the overweight group were significantly higher than those of normal group. In the case of female, 9.2% of the subjects were over-weight. This study shows that the implementation of weight control program is required at the worksites

• Journal of Nutrition and Health
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• v.35 no.7
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• pp.754-762
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• 2002

This study was performed to investigate the effect of sodium intake on blood pressure and blood parameters. 20 young adult women were fed the diets containing 290.5 mEq (high-Na diet) and 51.3 mEq (low-Na diet) Na for 6 days, respectively. BMI, DBP, and MBP were significantly lower in low-Na diet than those in high-Na diet. 20 subjects were divided into 3 groups according to the salt-sensitivity. In salt-sensitive group, decreases in SBP, DBP, and MBP by low-Na diet were shown. And there were not significant difference in blood pressure of salt-resistant group between high- and low-Na diet. In count-reactive group, MBP in low-Na diet was significantly higher than that in high-Na diet. Hemoglobin, creatinine, uric acid, and haptoglobin levels in serum were significantly higher in low-Na diet than those in high-Na diet. Among groups with different salt-sensitivity, increments of haptoglobin by low-Na diet were shown in salt-sensitive and counter-reactive groups. Actually, low sodium diet affects not only the blood pressure, but other biochemical parameters which in turn affect an individual overall health. Also salt-sensitivity should be considered as an important determinant. Therefore, for the patients who need restricted Na diet, it would be suggested that various biochemical changes and individual salt-sensitivity should be carefully considered along with dietary Na manipulation.

• Journal of electromagnetic engineering and science
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• v.1 no.2
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• pp.139-145
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• 2001

In this paper, a coaxial sample holder is proposed with its de-embedding and parameter extraction procedure. The S-parameters were measured up to 1 GHz using network analyzer, HP8753D, and N-type connector together with the de-embedding of N-type connector. The proposed de-embedding procedure is performed to extract electrical parameters of blood glucose, which gives the permittivity of blood glucose. We also analyzed the error of extracted parameters, which are caused by instrument error and geometrical error. Using these error analyses, we reduced the error factors of extracted parameters. We extracted electrical parameters of glucose samples through these all extraction procedure and confirmed the possibility of glucose diagnosis system based on microwave system.

• Annals of Laboratory Medicine
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• v.38 no.6
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• pp.503-511
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• 2018

Background: Sampling a healthy reference population to generate reference intervals (RIs) for complete blood count (CBC) parameters is not common for pediatric and geriatric ages. We established age- and sex-specific RIs for CBC parameters across pediatric, adult, and geriatric ages using secondary data, evaluating patterns of changes in CBC parameters. Methods: The reference population comprised 804,623 health examinees (66,611 aged 3-17 years; 564,280 aged 18-59 years; 173,732 aged 60-99 years), and, we excluded 22,766 examinees after outlier testing. The CBC parameters (red blood cell [RBC], white blood cell [WBC], and platelet parameters) from 781,857 examinees were studied. We determined statistically significant partitions of age and sex, and calculated RIs according to the CLSI C28-A3 guidelines. Results: RBC parameters increased with age until adulthood and decreased with age in males, but increased before puberty and then decreased with age in females. WBC and platelet counts were the highest in early childhood and decreased with age. Sex differences in each age group were noted: WBC count was higher in males than in females during adulthood, but platelet count was higher in females than in males from puberty onwards (P <0.001). Neutrophil count was the lowest in early childhood and increased with age. Lymphocyte count decreased with age after peaking in early childhood. Eosinophil count was the highest in childhood and higher in males than in females. Monocyte count was higher in males than in females (P <0.001). Conclusions: We provide comprehensive age- and sex-specific RIs for CBC parameters, which show dynamic changes with both age and sex.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2000.11a
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• pp.62-68
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• 2000

The aim of the study was to compare effects of music and white noise on the recovery of facial blood flow parameters after stressful visual stimulation. Twenty-nine subjects participated in the experiment. Three visual stimulation sessions with aversive slides (the IAPS, disgust category) were followed by subjectively "pleasant" (in the first session), "sad" music (in the second ), and white noise (in the third ). Order of sessions was counterbalanced. Blood flow parameters (peak blood flow, blood flow velocity, blood volume) were recorded by Laser Doppler single-crystal system (LASERFLO BPM 403A) interfaced through BIOPAC 100WS with AcqKnowledge software (v.3.5) and analyzed in off-line mode. Aversive visual stimulation itself decreased blood flow and velocity in all 3 sessions. Both "pleasant" and "sad" music led to the restoration of baseline levels in all blood flow parameters, while noise did not enhance recovery process. Music on post-stress recovery had significant change in peak blood flow and blood flow velocity, but not in blood volume measures. Pleasant music had bigger effects on post-stress recovery in peak blood flow and flow velocity than white noise. It reveals that music exerted positive modulatory effects on facial vascular activity measures during recovery from negative emotional state elicited by stressful slides. Results partially support the undoing hypothesis of Levenson (1994), which states that positive emotions may facilitate process of recovery from negative emotions.

• Journal of Nutrition and Health
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• v.39 no.5
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• pp.476-484
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• 2006

To elucidate the relationship between blood parameters related bone metabolism and antioxidant enzyme activity in postmenopausal period 60 women residing in Iksan area were recruited. Food and nutrient intake of each individual subject were estimated by 24-hour recalls of 3 non-consecutive days. The biochemical markers including total protein, albumin, osteocalcin (intact bone gla protein; BOP), calcium, phosphorus and hemoglobin were measured in fasting blood. In addition, parameters of antioxidative capacity including the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) , catalase (CAT) and total antioxidant capacity (TA) were monitored in blood, also. The mean age, height, weight, and BM! of subjects were 64.8 years, 151.1 em, 59.5 kg $26.0\;kg/m^2$, respectively. The mean SOD, GPx, and CAT activities were 138.5 U/ml, 1,273.8 U/ml and 314.3 kU/l respectively, and TA was 1.16 mmol/l without significant difference among different age groups. BMI was positively correlated with SOD activity (p < 0.01). SOD activity and CAT activity showed positive correlation with serum albumin (p < 0.05) and hemoglobin (p < 0.01). In conclusion, this study revealed that antioxidant enzyme activity holds a significant relationship with the blood parameters like as serum albumin and hemoglobin in postmenopausal women and further systematic research is needed to investigate the their relation mechanism.

• Korean Journal of Veterinary Research
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• v.45 no.1
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• pp.121-125
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• 2005

The present study was carried out to produce the hematological and blood chemical findings after hypophysectomy in rats. Hypophysectomy was performed by the parapharyngeal method and the sham surgery was performed for the control group. Two weeks after the operation, the body weight of the hypophysectomized and control rats was measured daily for 5 days. We deleted the rats the weight gain of which is less than 5 g during 5 days from the hypophysectomy group. The successful operation rate was approximately 40%. In the hypophysectomized and control rats, their blood samples were collected from posterior vena cava after celiotomy under generally anesthesia with ether. Hematological parameters such as erythrocyte count, leukocyte count, hemoglobin concentration, hematocrit level, and platelet count were determined by Animal Blood Counter. The erythrocyte count, hemoglobin concentration, and hematocrit level were lower significantly (p<0.01), and the leukocyte count was lower significantly (p<0.05) in hypophysectomy group compared with control group. But the plate count did not show significant difference (p>0.05) between hypophysectomy group and control group. Also, blood chemical parameters such as glucose, blood urea nitrogen (BUN), aspartate animotransferase, albumin, total protein, cholesterol, calcium, and magnesium in serum were determined. Except BUN concentration, all parameters were not affected by hypophysectomy. But the BUN concentration was higher significantly (p<0.01) in hypophysectomy group compared with control group.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.6
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• pp.1159-1165
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• 2011

Telemetry techniques of rats have been used for assessing safety pharmacology of drugs and chemicals. Biological signals including blood pressure and heart rate measured under anesthesia were significantly different from those obtained under normal conditions. The stress of restraint in awake animals can also affect the accuracy of physiological evaluation. This paper details the surgery required to allow key cardiovascular parameters to be determined. The telemetric measurement of cardiovascular parameters such as blood pressure, heart rate, electrocardiograph(ECG) established. We carried out the continuous monitoring of cardiovascular parameters using the telemetry system in F344 rats. During the measurement, no significant changes were observed in the heart rate and blood pressure. ECG signals and body temperature were also constant during the measurement of biological signals. With the results of this study, we conclude that this telemetry system can be applied usefully for the assesment of biological parameters in the rats.