• Title/Summary/Keyword: brassicaceae

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An Unrecorded Naturalized Plant in Korea : Cakile edentula (Brassicaceae) (미기록 귀화식물: Cakile edentula (Brassicaceae))

  • Kil, Ji-Hyon;Lee, Kyu Song
    • Korean Journal of Plant Taxonomy
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    • v.38 no.2
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    • pp.179-185
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    • 2008
  • Unrecorded species from the beach at Gangnung City is reported: Cakile edentula, Brassicaceae. The genus Cakile is unrecorded in Korea and easily distinguished from other genera in Brassicaceae by the upper member of fruits distinctly longer or thicker than the lower part, petiole white or anthocyanic, style absent and leaves glabrous. The local name is Seo-yang-gaet-naeng-i and the morphological characters and illustrations of the species are provided.

Advances in in vitro culture of the Brassicaceae crop plants

  • Park, Jong-In;Ahmed, Nasar Uddin;Kim, Hye-Ran;Nou, Ill-Sup
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.13-22
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    • 2012
  • Plant regeneration has been optimized increasingly by organogenesis and somatic embryogenesis using a range of explants with tissue culture improvements focusing on factors, such as the age of the explant, genotype, media supplements and $Agrobacterium$ co-cultivation. The production of haploids and doubled haploids using microspores has accelerated the production of homozygous lines in Brassicaceae crop plants. Somatic cell fusion has facilitated the development of interspecific and intergeneric hybrids in sexually incompatible species of $Brassica$. Crop improvement using somaclonal variation has also been achieved. Transformation technologies are being exploited routinely to elucidate the gene function and contribute to the development of novel enhanced crops. The $Agrobacterium$-mediated transformation is the most widely used approach for the introduction of transgenes into Brassicaceae, and $in$ $vitro$ regeneration is a key factor in developing an efficient transformation method in plants. Although many other Brassicaceae are used as model species for improving plant regeneration and transformation systems, this paper focuses on the recent technologies used to regenerate the most important Brassicaceae crop plants.

Progress in Genetic Manipulation of the Brassicaceae

  • Ahmed, Nasar Uddin;Park, Jong-In;Kim, Hye-Ran;Nou, Ill-Sup
    • Journal of Plant Biotechnology
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    • v.39 no.1
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    • pp.1-12
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    • 2012
  • With the increasing advances in Brassicaceae genetics and genomics, considerable progress has been made in the transformation of Brassicaceae. Transformation technologies are now being exploited routinely to determine the gene function and contribute to the development of novel enhanced crops. $Agrobacterium$-mediated transformation remains the most widely used approach for the introduction of transgenes into Brassicaceae. In $Brassica$, the transformation relies mainly on $in$ $vitro$ transformation methods. Nevertheless, despite the significant progress made towards enhancing the transformation efficiencies, some genotypes remain recalcitrant to transformation. Advances in our understanding of the genetics behind various transformations have enabled researchers to identify more readily transformable genotypes for use in routine high-throughput systems. These developments have opened up exciting new avenues to exploit model $Brassica$ genotypes as resources for understanding the gene function in complex genomes. Although many other Brassicaceae have served as model species for improving plant transformation systems, this paper summarizes on the recent technologies employed in the transformation of both $Arabidopsis$ and $Brassica$. The use of transformation technologies for the introduction of desirable traits and a comparative analysis of these as well as their future prospects are also important parts of the current research that is reviewed.

First record of invasive species Alliaria petiolata (M. Bieb.) Cavara & Grande (Brassicaceae) in Korea (한국 미기록 귀화식물: 마늘냉이(십자화과))

  • Cho, Seong-Hyun;Kim, Young-Dong
    • Korean Journal of Plant Taxonomy
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    • v.42 no.4
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    • pp.278-281
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    • 2012
  • Alliaria petiolata (M. Bieb.) Cavara & Grande (Brassicaceae) is an invasive species which is native in Europe and SW Asia. This species is currently invading the understory of mature temperate forests of North America. In Korea, A. petiolata is found to invade and colonize areas at forest margins along roadsides (Samcheok- si, Gangwon-do). This initial investigation serves to inform of the importance of early detection and extermination of this particular weed in Korea.

One-step Multiplex RT-PCR Method for Simultaneous Detection of Seed Transmissible Bacterium and Virus Occurring on Brassicaceae Crop Seeds (십자화과 작물 종자에서 종자전염 세균 및 바이러스 동시 검출을 위한 One-step Multiplex RT-PCR 방법)

  • Jeong, Kyu-Sik;Soh, Eun-Hee
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.52-58
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    • 2011
  • The aim of this research was to develop specific and sensitive PCR-based procedures for simultaneous detection of economically important plant pathogenic bacteria and seed borne virus in commercial Brassicaceae crop seeds, Xanthomonns campestris pv. campestris (Xcc) and Lettuce Mosaic Virus (LMV). Bacterial and virus diseases of Brassicaceae leaves are responsible for heavy losses. PCR with arbitral primers: selection of specific primers, performance of PCR with specific primers and determination of the threshold level for pathogens detection. To detect simultaneously the Xcc and LMV in commercial Brassicaceae crop seeds (lettuce, kohlrabi, radish, chinese cabbage and cabbage), two pairs of specific primer (LMV-F/R, Xcc-F/R) were synthesized by using primer-blast program (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). The multiplex PCR for the two pathogens in Brassicaceae crop seeds could detect specifically without interference among primers and/or cDNA of other plant pathogens. The pathogen detection limit was determined at 1 ng of RNA extracted from pathogens. In the total PCR results for pathogen detection using commercial kohlrabi (10 varieties), lettuce (50 varieties), radish (20 varieties), chinese cabbage (20 varieties) and cabbage (20 varieties), LMV and Xcc were detected from 39 and 2 varieties, respectively. In the PCR result of lettuce, LMV and Xcc were simultaneously detected in 8 varieties.

Screening Method for Non-viable Seeds in Brassicaceae Vegetable Crops by Sinapine Leakage (Sinapine누출을 이용한 십자화과 채소의 퇴화종자 선별법)

  • 민태기
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.39 no.5
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    • pp.473-479
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    • 1994
  • A seed coating system was developed to screen non-viable seeds in the Brassicaceae. The crops studied were radish, chinese cabbage, broccoli, cauliflower and brussel sprout. Sinapine leaked more from artificially deteriorated seeds than non-deteriorated seeds. Seed coating with cellulose was to trap the sinapine leakage in the non-viable Brassicaceae vegetable seeds. The seeds were first hydrated, then coated with cellulose powder to capture the leakage. Coated seeds were dried, then sorted two fractions-fluorescent seeds and non-fluorescent seeds under the UV light. The ratio of the fluorescent seeds were higher in bad seedlot than good one. The germination rate were increased 3∼35% by eliminating the fluorescent seeds in tested Brassica vegetable seeds. Sowing non-fluorescent seeds resulted in a greater percent normal seedling than non-coated seeds in all seedlots. The fluorescent seeds contained a high percentage of the dead and abnormal seedlings.

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Rorippa apetala: A new species of Rorippa Scopoli. (Brassicaceae) from Korea (섬강개갓냉이: 한국에서 발견된 개갓냉이속(배추과)의 1신종)

  • Kim, Yoon-Young;Ji, Seong-Jin;Oh, Byoung-Un
    • Korean Journal of Plant Taxonomy
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    • v.40 no.2
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    • pp.84-89
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    • 2010
  • Herein is described a new species of Rorippa (R. apetala, Brassicaceae) discovered on the River Seom-gang in Wonju-si, Gangwon-do, Korea. The new species is distinct from the closely-related species, R. palustris and R. globosa, by having adventitious buds on the surface of rhizome-type roots during the fruiting season, usually absent or rarely 1-4, if present then atrophy petals, obpandurate fruits, and seeds usually empty or rarely 1-2 per locule. The key to these species is provided.

The Role of a Floral Identity Gene LFY in Plant Morphological Evolution

  • Park, Young-Doo;Yoon, Ho-Sung
    • Korean Journal of Plant Taxonomy
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    • v.37 no.4
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    • pp.323-333
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    • 2007
  • The degree to which parallel evolution utilizes the same genetic mechanisms indicates the degree to which developmental processes constrain or channel phenotypic evolution. A transgenetic strategy was used to elucidate the role of one floral meristem identity gene, LEAFY (LFY), in the evolution of rosette flowering, a plant architecture that has evolved in parallel in several lineages of the mustard family, Brassicaceae. The LFY genes from three rosette flowering species were cloned and introduced into a species with the ancestral architecture, and results indicated that changes at the LFY locus contributed to the evolution of rosette flowering in two of the three lineages, but that in each lineage a different set of genetic partners was involved. Also, LFY was shown to play a role in the evolution of flower size. Transgenetic strategy may be useful in the study of plant morphological evolution and parallelism.

The complete mitochondrial genome of Arabidopsis thaliana (Brassicaceae) isolated in Korea

  • PARK, Jongsun;XI, Hong;KIM, Yongsung
    • Korean Journal of Plant Taxonomy
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    • v.51 no.2
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    • pp.176-180
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    • 2021
  • Arabidopsis thaliana (L.) Heynh. is a small plant species that serves as a model organism of plant biology and genetics. Here, we present the first complete mitochondrial genome of Korean A. thaliana natural isolate (named as 180404IB4), which is 368,875 bp long and contains 58 genes (33 protein-coding genes, 22 tRNAs, and three rRNAs), with a GC ratio of 44.8%. Sixty-four single-nucleotide polymorphisms and 11 insertion and deletion regions (1,089 bp in length) are identified against the Col-0 ecotype, showing one large insertion of 1,069 bp without structural variation. Phylogenetic trees constructed from 30 conserved genes indicate that the 180404IB4 mitochondrial genome is clustered with Col-0 and three East Asian ecotypes.