• Title, Summary, Keyword: brucellosis

Search Result 128, Processing Time 0.029 seconds

The latent period and anti-epidemic measure of bovine brucellosis (소 부루세라병 잠복기간과 방역대책;-제주도 소 부루세라병 근절대책을 중심으로-)

  • 김종성
    • Korean Journal of Veterinary Service
    • /
    • v.20 no.1
    • /
    • pp.1-9
    • /
    • 1997
  • The 37,000 holstein which had been raised in Jeju island were examined for the purpose of brucellosis detection and so on. We investigated the relationship between the farms showing positive outbreak and the number of examination, incubation period, and the outbreak rate of positive cattle. We endeavored to separate germs from brucellosis positive cattles blood and negative bulls semen against brucellosis serum test and concluded as follows. We intend to offer you institutional supplements and preventions of brucellosis for the future. 1. Outbreak of brucellosis could not lead the outbreak rate of positive cattle to decrease through 1-2 examination per year, For the past 4 years, the outbreak rate of brucellosis positive cattle had a tendency to show a gradual decrease through at least 5 examinations per year. 2. As we examined live-in cattle with positive cattle in mass outbreak farms for 9 months, we found out positive cattle every month. In a grazing land, the group of cattle producd enormous positive cattle in 9-12 months. 3. Annual brucellosis positive outbreak rate was 20-25% among live-in cattle with brucellosis positive cattle, but the rate might be 50-100% depending on extent of pollution. 4. 94's brucellosis examination showed that 200 positive cattle of 71,153 cattle and most of them were live-in cattle with confirmed brucellosis positive cattle. 5. 1 head of 200 positive cattle showed positivity against serum of bulls test and the rate of bulls was about 1%. 6. Brucellosis germ was separated from 2 cattle's semen among 52 negative bulls against brucellosis serum test and there was the one brucellosis positive cattle against tube agglutination of semen test, so the positive rate of brucellosis was 5.8%. 7. Brucellosis germ was not separated from 15 brucellosis positive cattle's blood.

  • PDF

Evaluation on diagnostic efficiency of the standard tube agglutination test for bovine brucellosis (소 브루셀라병 표준시험관응집반응법 진단효율 평가)

  • Sung, So-Ra;Kim, Ji-Yeon;Her, Moon;Lee, Kichan;Gu, Jeong-Hui;Kang, Sung-Il;Lee, Hyang-Keun;Kim, Suk-Mi;Jung, Suk-Chan
    • Korean Journal of Veterinary Service
    • /
    • v.35 no.4
    • /
    • pp.269-273
    • /
    • 2012
  • A confirmatory serological test, the standard tube agglutination test (STAT) is evaluated for the diagnostic efficiency in brucellosis Korea. A total of 345 bovine samples were collected from regional veterinary branch under national brucellosis monitoring program from January 2010 to June 2012 in Korea. These samples were diagnosed as suspected serum and brucellosis positive by the Rose Bengal test (RBT) and the STAT, respectively. The STAT was compared and evaluated with three serological test such as the indirect-enzyme linked immunosorbent assay (I-ELISA), competitive-enzyme linked immunosorbent assay (C-ELISA) and fluorescence polarisation assay (FPA) prescribed for international trade by OIE. Among the 345 bovine serum samples, 302 (87.5%) were diagnosed as positive in the STAT, while 215 (62.3%), 223 (64.6%) and 194 (56.2%) serum samples were diagnosed as positive for brucellosis in the I-ELISA, C-ELISA and FPA, respectively. The STAT showed quite high positive results as compared with three prescribed tests of OIE. FPA, I-ELISA and C-ELISA have shown 60.6%, 64.9% and 67.2% correlation, respectively as compared to the STAT. However correlations of three prescribed tests ranged high 84.1~97.7%. Especially, correlation between I-ELISA and C-ELISA is quite high, 97.7%. These results suggest that the STAT has shown many false-positive reactions. Therefore, additional serological test, such as ELISAs and FPA, would be necessary to adopt as a confirmatory test in the national surveillance program of bovine brucellosis in Korea.

Serological monitoring on brucellosis in livestock of Korea (국내 가축에서 브루셀라병에 대한 혈청학적 모니터링)

  • Sung, So-Ra;Kim, Ji-Yeon;Her, Moon;Lee, Kichan;Kang, Sung-Il;Lee, Hyang-Keun;Cho, Hyo Rim;Lee, Jin Ju;Jung, Suk Chan
    • Korean Journal of Veterinary Research
    • /
    • v.54 no.4
    • /
    • pp.197-201
    • /
    • 2014
  • In Korea, brucellosis has been reported periodically in cattle and rarely in dogs; however, it has not previously been screened in domestic animals such as elk, pigs and goats. To investigate the serological prevalence, serum samples were taken from the aforementioned animals annually during 2007-2013 and screened by the rose-bengal test (RBT) or modified RBT, after which positive sera were evaluated by the standard tube agglutination test (STAT). Finally, RBT and STAT-positive sera were confirmed by competitive-ELISA. Brucella abortus biovar 1 was isolated from three elk that were shown to be positive serologically in 2008. There was no evidence of brucellosis in pigs. Based on serological monitoring and investigation of etiological agents, there is no evidence of outbreak of brucellosis in elk, pigs or goats of Korea since 2008. However, the possibility for brucellosis from cattle to affect these other livestock exists; therefore, extensive and continuous serological monitoring is required to maintain their brucellosis-free status.

Investigation of occurrence factors on brucellosis-outbreak farm in Korea (소 브루셀라병 동시 다두 발생 농장의 발생 요인 조사)

  • Kim, Ji-Yeon;Kang, Shin-Seok;Her, Moon;Lee, Kichan;Sung, So-Ra;Gu, Jung-Hui;Kang, Sung-Il;Lee, Hyang-Keun;Kim, Yu-Jin;Kim, Dong-Gil;Jung, Suk-Chan
    • Korean Journal of Veterinary Service
    • /
    • v.35 no.4
    • /
    • pp.263-268
    • /
    • 2012
  • From the end of July 2012, several cases of abortion have been happened at the Korean indigenous cattle farm with 124 heads in Chungbuk province, Korea. Serological tests such as Rose-bengal test (RBT) and standard tube agglutination test (STAT) have been performed according to the standard official protocols of bovine brucellosis and 41 cattle turned out to be brucellosis-positive simultaneously. To find out the main factors of brucellosis outbreaks and spreads, additional serological, etiological and molecular investigation were applied. Totally, 11 B. abortus were isolated from 10 cattle's specimens including lymph-nodes and/or testis, and drinking water in cowhouse. In genotyping by multi-locus VNTR assay (MLVA) using 17 loci markers, the present B. abortus isolates were shown all the same pattern, D1 genotype, which has been reported in Gyeonggi and Gangwon province, Korea. These results suggest that the input of brucellosis might come from neighboring farms directly or indirectly, even if by unknown factor and expansion within farm would accelerate by materials related with aborting cows.

Seroprevalence of brucellosis in cattle in selected area of Bangladesh and comparison between Rose Bengal test and i-ELISA used for the screening of brucellosis

  • Rahman, Md. Siddiqur;Chakrabartty, Amitavo;Islam, Md. Taohidul;Sarker, Roma Rani;Alam, M.E.;Uddin, Muhammad Jasim;Akther, Laila;Song, Hee-Jong
    • Korean Journal of Veterinary Service
    • /
    • v.35 no.2
    • /
    • pp.133-137
    • /
    • 2012
  • Brucellosis, a bacterial zoonoses caused by the genus Brucella is responsible for abortion and infertility in cow. Brucellosis is causing economic loss in dairy industries and prevalent worldwide including Bangladesh but limited studies are devoted to determine the prevalence and its association with reproductive factors of dairy cows in Bangladesh. Therefore, the present study was conducted to determine the seroprevalence of brucellosis in dairy cattle using screening test Rose Bengal test (RBT) and the positive sera were further confirmed by indirect- ELISA. For this purpose, a total of 400 serum samples from dairy cows with history of abortion and various reproductive disorders were collected from the Kurigram district of Bangladesh for the detection of Brucella antibody. The overall prevalence of brucellosis in dairy cattle was 2.25%. Brucellosis in cases of abortion and repeat breeding was 8.3% and 2.8%, respectively. The results shows higher prevalence of brucellosis in cases of abortion followed by repeat breeding, while there was no seropositive cases from other reproductive disorders. Age-wise sero-prevalence was found 3.0% in 2~3 years age group and 2.0% in 4~8 years age group. The prevalence of brucellosis in indigenous and cross-bred cattle was 3.6% and 1.7%, respectively. All the animals detected positive to brucellosis by RBT were not found to be positive by i-ELISA. However, the RBT might be a suitable screening test for the diagnosis of Brucella infection in field condition in Bangladesh. These data will help to develop effective disease prevention strategies.

Brucellosis in sheep and goat of Bogra and Mymensingh districts of Bangladesh

  • Rahman, Md. Siddiqur;Hahsin, Md. Ferdous Ali;Ahasan, Md. Shamim;Her, Moon;Kim, Jeong Yeong;Kang, Sang Il;Jung, Suk Chan
    • Korean Journal of Veterinary Research
    • /
    • v.51 no.4
    • /
    • pp.277-280
    • /
    • 2011
  • Brucellosis is the most important bacterial disease of livestock in Bangladesh. The present study was conducted to determine the seroprevalence of brucellosis in goat and sheep in Mymensingh and Bogra districts of Bangladesh using slow agglutination test and Rose Bengal test as screening test and indirect enzyme linked immunosorbent assay as confirmatory test. Questionnaire based data on age, gender, area, client's complaint, number of animals in herds, disease history, reproductive problems such as abnormal uterine discharge, abortion or previous abortion in sheep and goat and their reproductive diseases were recorded. A total of 200 sera samples were collected from 80 sheep and 120 goats. The prevalence of brucellosis in goat was 2.50% and 1.25% in sheep. Positive reactors were only detected in female of both goat and sheep. In this study, there existed a significant association among abortion and the prevalence of brucellosis (p < 0.01). The prevalence of brucellosis in sheep and goat in Bangladesh is not negligible, and it is therefore worth considering the adoption of preventive measures.

A Discrete Model of Brucellosis Happened in Korean Livestock Farms

  • Park, Junpyo;Kim, Byul Nim
    • Kyungpook Mathematical Journal
    • /
    • v.47 no.4
    • /
    • pp.601-608
    • /
    • 2007
  • In this paper we introduce a discrete model of brucellosis happened in Korean livestock farms and numerically analyze its dynamical features. To do it, we consider parameters data supported by Livestock Cooperatives. To control brucellosis, we investigate the relationship among key parameters, as applications of our model. We hope that our model may be used to reduce brucellosis in Korean livestock farms.

  • PDF

Seroprevalence of Brucellosis among Risk Population in Gyeongsangbuk-do, 2006 (경상북도 거주 고위험군에서 브루셀라 감염에 관한 혈청 역학적 연구)

  • Lee, Kwan;Park, Woo-Won;Kim, Sung-Hwan;Lee, Do-Young;Park, Mi-Yeoun;Hur, Young-Ju;Lim, Hyun-Sul
    • Journal of Preventive Medicine and Public Health
    • /
    • v.40 no.4
    • /
    • pp.285-290
    • /
    • 2007
  • Objectives : Cases of human brucellosis in Korea have recently increased due to the increasing incidence of bovine brucellosis. The authors conducted this study to elucidate the status of brucellosis through seroepidemiologic study. Methods : We selected our study population from a high risk group. We conducted a questionnaire survey and obtained blood samples to determine the seroprevalence of brucellosis antibodies for 10 days in February, 2005. The titers of brucellosis were measured by the combination of standard tube agglutination test (STA) and enzyme-linked immunosorbent assay (ELISA) test. Results : Our study subjects comprised 1,075 cases: 971 livestock workers, 51 veterinarians, and 53 artificial inseminators. In the STA test, 27 cases (2.5%) had titers of greater than or equal to 1:20. Of 1,068 cases (7 cases were excluded due to previous brucellosis), 7 cases of brucellosis were diagnosed with titers of 1:160, giving a seroprevalence of brucellosis of 0.66%. The seroprevalence in the male group was 0.95%, and that of livestock workers, veterinarians, and artificial inseminators was 0.52%, 4.17%, and 0.00%, respectively. The Spearman's correlation coefficient between the positive rate of bovine brucellosis per capita and household and human brucellosis was 0.806 and 0.744, respectively. The concordance rate between the Korea National Institute of Health and the Gyeongsangbuk-do Institute of Health and Environment by the STA and ELISA tests was 94.7% and 100.0%, respectively. Conclusions : The study results indicated in higher seroprevalence rate among veterinarians than among livestock workers and artificial inseminators. Because veterinarians may be exposed to this high risk, effective working guidelines for veterinarians to guard against brucellosis must be developed. Moreover, more extensive epidemiologic research for laboratory workers and meat handlers is needed.

Indirect enzyme linked immunosorbent assay for the diagnosis of brucellosis in cattle

  • Rahman, Siddiqur;Huque, Fazlul;Ahasan, Shamim;Song, Hee-Jong
    • Korean Journal of Veterinary Service
    • /
    • v.33 no.2
    • /
    • pp.113-119
    • /
    • 2010
  • Brucellosis is a major zoonosis caused by Gram negative facultative intracellular bacterial organisms of the genus Brucella that are pathogenic for a wide variety of animals and human beings. Because of its economic impact on animal health and the risk to the human population,most countries have a brucellosis control program. Brucellosis is also an economically important andprevalent disease in Bangladesh. The accurate and prompt diagnosis is very important in controlling and eradicating of the disease in animals. The present study was undertaken to determine the seroprevalence of brucellosis in cattle in Mymensingh and Patuakhali district of Bangladesh. A total of 120 serum samples were collected from the two districts along with a questionnaire related to the epidemiology of the disease. The sampleswere screened by using slow agglutination test and conformed by indirect enzyme linked immunosorbent assay. The overall seroprevalence of brucellosis in cattle was 5% and it was observed that, a higher prevalence of Brucella was found in female than male, through natural breeding than artificial insemination (AI) and animal above 4 years old are highly susceptible than younger ones. Higher prevalence was found in aborted animals in comparison with non aborted animal. Finally, the study revealed that the female animal has more susceptible to brucellosis and healthy semen should be used for AI.

Seroprevalence of specific Brucella infection of cattle in Bangladesh Agricultural University Veterinary Clinics and its surrounding areas

  • Rahman, Md. Siddiqur;Alam, Nur;Rahman, A.K.M. Anisur;Huque, A.K.M. Fazlul;Ahasan, Md. Shamim;Song, Hee-Jong
    • Korean Journal of Veterinary Service
    • /
    • v.32 no.3
    • /
    • pp.219-225
    • /
    • 2009
  • A cross sectional survey was conducted to determine the seroprevalence of brucellosis in cattle in Bangladesh Agricultural University (BAU) Veterinary Clinics, in BAU Dairy Farm and Vabokhali from June 2008 to November 2008. A total of 200 serum samples were collected from BAU Veterinary Clinic, from BAU Dairy Farm and Vabokhali. Among the serum samples 143 sera samples were collected from BAU Veterinary Clinic, 42 serum samples from BAU Dairy Farm and 15 serum samples from Vabokhali. Sera were separated from blood samples and tested with specific Brucella abortus antigen (BAA) test and B. melitensis antigen (BMA) test. The overall seroprevalence of brucellosis in cattle was 5% in BAA and 0.5% in BMA. It was observed that, a significant higher prevalence of B. abortus was found in female than male. An insignificant higher prevalence of brucellosis was found in adult cattle (aged above 5 years), in cross breed cattle, in cattle with grazing, cattle breed by natural breeding, and in pregnant cows. Although insignificant but a higher prevalence of brucellosis was found in aged cattle than young cattle, cross bred cattle, pregnant cattle than non pregnant cattle, cattle with grazing. A higher prevalence of brucellosis was found in female cattle than male.