• Title/Summary/Keyword: c-Fos

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Efficient Flooding Mechanisms with Considering Data Transmission Characteristics of the Wireless Sensor Network (무선 센서 네트워크의 데이터 전송 특성을 고려한 효율적인 플러딩 기법)

  • Ahn, Sang-Hyun;Lim, Yu-Jin;Kim, Mahn-Hee
    • The KIPS Transactions:PartC
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    • v.16C no.2
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    • pp.209-216
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    • 2009
  • In the wireless sensor network, flooding is required for the dissemination of queries and event announcements. The simple flooding causes the implosion and the overlap problems, so the simple flooding may result in the reduced network lifetime. Therefore, in this paper, we propose the flooding overlay structure (FOS) so that the overhead caused by flooding can be reduced. We propose two variants of FOS mechanisms, the centralized FOS (CFOS) and the distributed FOS (DFOS). In CFOS, the sink collects the network topology information and selects forwarding nodes based on that information. On the other hand, DFOS allows each sensor node to decide whether to act as a forwarding node or not based on its local information. For the performance evaluation of our proposed mechanisms, we carry out NS-2 based simulations and compare ours with the simple flooding and the gossiping. The simulation results indicate that the proposed FOS mechanisms outperform the simple flooding in terms of the network lifetime and the gossiping in terms of the data delivery ratio.

Alterations of c-Fos mRNA Expression in Hypothalamic-Pituitary-Adrenal Axis and Various Brain Regions Induced by Intrathecal Single and Repeated Substance P Administrations in Mice

  • Choi, Seong-Soo;Lee, Han-Kyu;Shim, Eon-Jeong;Kwon, Min-Soo;Seo, Young-Jun;Lee, Jin-Young;Suh, Hong-Won
    • Archives of Pharmacal Research
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    • v.27 no.8
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    • pp.863-866
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    • 2004
  • The effect of substance P (Sub P) injected intrathecally (I.t.) on c-fos mRNA expression in vari-ous tissues was examined in the present study. We found that a single administration of Sub P(0.5 nM) caused an increase of the c-fos mRNA level in the hypothalamic-pituitary-adrenal(HPA) axis, hippocampus, and spinal cord. The time-course study showed that c-fos mRNA level was maximal at 10 min and began to decrease 30 min after the Sub P injection in all tis-sues, and the Sub P-induced increase of the c-fos mRNA level was returned to the control level 1 h after the injection. The kinetics of the c-fos mRNA expression in mice that were repeatedly injected with Sub P (every 30 min interval up to 4 times) were different in the HPA axis, hippocampus, and spinal cord. The increased c-fos mRNA level in the hypothalamus and the spinal cord induced by I.t. injected Sub P remained at a high level. In the pituitary gland, adrenal gland, and hippocampus, the increased level of c-fos mRNA expression gradually returned to the control level during the repeated substance P injections up to 4 times. Our results suggest that spinally injected Sub P-induced pain stress increases c-fos mRNA expres-sion in the spinal cord, hippocampus, and HPA axis. In mice repeatedly injected with Sub P, the kinetics of c-fos mRNA appear to be different varied from tissue to tissue.

Effect of Steroid Hormones on the Expression of c-Fos, CREB, ATF, and HSP70 in Rat Uterus (흰쥐 자궁에서 스테로이드호르몬에 의한 c-Fos, CREB, ATF 및 HSP70의 발현에 관한 연구)

  • Lee, Young-Ki;Kim, Sung-Rye
    • Clinical and Experimental Reproductive Medicine
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    • v.25 no.3
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    • pp.305-313
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    • 1998
  • Steroid hormone is known to cause the dynamic changes of mammalian uterus during reproductive cycle. However there is little information about the effect of estrogen (E) and progesterone (P) on the expression of various transcription factors involved in gene expression. Thus the present study was designed to demonstrate E and/or P-induced expression of c-Fos, CREB, ATF and HSP70 in rat uterus. Rats, ovariectomized (OVX) for two weeks, were divided into 6 experimental groups, 1) OVX, 2) OVX+V, 3) OVX+E, 4) OVX+P, 5) OVX+E+V, 6) OVX+E+P, and western blotting assay for nuclear extract and immunohistochemical staining were carried out for each experimental group. Treatment of E $(10{\mu}g)$ showed to increase the expression of c-Fos, CREB, ATF, and HSP70, and maximal expression was occured at $3\sim6$ hr after E administration. P (1mg) also increased, but much less than E, the expression of c-Fos, ATF, and HSP70. However, P did not reveal any effect on the expression CREE. P treatment 4 hr after E injection decreased c-Fos, CREB, and ATF expression, but did not show any change in the E-induced HSP70 expression. In immunohistochemical study c-Fos-, CREB-, and ATF-immunoreactivities were confined to the cells of luminal epithelium of uterine endometrium. These results suggest that proliferation and differentiation of rat uterus during reproductive cycle may mediated via expression of transcription factors, such as c-Fos, CREB, ATF, and HSP70.

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Effects of Oral Administration of Ligigeopoongsan on Neuropathic Pain and c-Fos Protein Expression in Rats (이기거풍산(理氣祛風散)이 백서(白鼠)의 신경병리성(神經病理性) 동통(疼痛) 억제(抑制) 및 c-Fos 단백(蛋白) 발현(發顯)에 미치는 영향(影響))

  • Lee, Hyek-Je;Kim, Jong-Han;Park, Su-Yeon;Choi, Jung-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.18 no.1
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    • pp.50-60
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    • 2005
  • Objective : We have studied to know effects of Ligigeopoongsan(LGS, 理氣祛風散) on mechanical allodynia, cold allodynia and c-Fos protein expression in the model of neuropathic pain of rats. Methods : The model of neuropathic pain was made by injured tibial nerve and sural never while common peroneal never was maintained. After 2weeks, we performed behavioral test for 7 days to try out mechanical allodynia using von frey filament and cold allodynia using acetone, which are calculated by counting withdrawal response on foot. Rat brains removed and sliced on 8th days. Serial sections were immunohistochemically reacted with polyclonal c-Fos antibody. The numbers of c-Fos protein immunoreactive neurons in the central gray were examined using scion image program. Results : 1. Mechanical allodynia in LGS-2, LGS-3 groups were significantly diminished compared with the control group. 2. Cold allodynia in LGS-3 group was significantly diminished compared with the control group. 3. c-Fos protein expression on the central gray LGS-2, LGS-3 groups were significantly lower than that of control group. conclusions : We have noticed that LGS(理氣祛風散) diminished mechanical and cold allodynia in the model of neuropathic pain compared with the control group. c-Fos protein expression in the central gray of that group was also decreased compared with the control group. Pain control group were LGS was accumulated time goes by. This study can be used as a basic resource on a study and a treatment of pain.

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Influence of Transcutaneous Electrical Nerve Stimulation and Electroacupuncture on C-fos Expression in Spinal Cord and Functional Recovery After Rat Sciatic Nerve Crush Injury (경피신경전기자극과 전침자극이 흰쥐 좌골신경 압좌손상 후 척수내 c-fos 발현과 기능회복에 미치는 영향)

  • Lee, Hyun-Min
    • The Journal of the Korea Contents Association
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    • v.9 no.6
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    • pp.187-195
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    • 2009
  • The purpose of this study was to identify the effect of transcutaneous electrical nerve stimulation(TENS) and electroacupuncture(EA) after sciatic nerve crush injury in rats. Subjects were classified TENS group with TENS application, EA group with EA application and Control group which is not applicated electrical stimulation. TENS and EA stimulations were applied post-injury day(PD) 1 to 14 after sciatic nerve injury. This study observed c-fos expression in rat lumbar spinal cord. In addition, the paw withdrawal latency(PWL) and sciatic function index(SFI) were measured. The results were as follows: At PD 1, control group had higher c-fos immunoreactivity than experimental groups. At PD 7 and 14, control group had higher c-fos immunoreactivity than experimental groups. The PWL of experimental groups were significantly lower than control group. The SFI had not significant difference in all groups. But the average of experimental groups were higher than control group. These results suggest that TENS and EA applications increasing sensory and motor nerve recovery while decreasing c-fos immunoreactivity after sciatic nerve crush injury.

C-fos mRNA Expression in Rat Hippocampal Neurons by Antidepressant Drugs (배양한 흰쥐 해마신경세포에서 항우울제에 의한 c-fos mRNA의 발현)

  • Park, Eung-Chul;Cho, Yun-Gyoo;Yang, Byung-Hwan;Kim, Kwang-Iel;Yang, Bo-Gee;Chai, Young-Gyu
    • Korean Journal of Biological Psychiatry
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    • v.8 no.1
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    • pp.85-95
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    • 2001
  • This study was designed to examine the effects of two antidepressant drugs on the expression of c-fos mRNA in cultured embryonic rat hippocampal neurons. The drugs used were imipramine and amitriptyline. On the fourth day of culture, hippocampal neurons were treated with variable concentrations of each drug. Competitive RT-PCR(Reverse Transcriptase-PCR) analysis was used to quantify the c-fos mRNA expression induced by each drug. Experimental results showed that acute and direct treatment with imipramine and amitriptyline with relatively low concentrations(imipramine ${\leq}10{\mu}M$, amitriptylne ${\leq}10{\mu}M$) had no inductive effect on the expression of c-fos mRNA in the rat hippocampal neurons. However, after treatment with relatively high concentrations(imipramine ${\geq}100{\mu}M$, amitriptyline ${\geq}100{\mu}M$) c-fos mRNA was not detected. These findings suggest the followings. Firstly, the action mechanisms of these drugs on the hippocampal neurons might not be mediated by c-fos but by other immediate-early genes(IEGs). Secondly, their actions may be mediated indirectly via other areas of the brain. Thirdly, the expression of c-fos might be inhibited by high concentrations of these drugs, or the high concentrations could induce cell death. Finally, though cell death remains to be confirmed, the inhibition of c-fos induction or cell death could play a role in the cognitive impairments known to be adverse effects of some antidepressants. This study is believed to be a first step toward understanding the mechanisms of learning and memory. Further studies are needed to investigate the expression of various IEGs and changes in the hippocampal neurons of rat resulting from chronic treatment with antidepressant drugs.

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Effect of Electroacupuncture of GB30 on the Expression of c-Fos following Transient Forebrain Ischemia in Rats (환도 (GB30) 전침자극이 일과성 전뇌 허혈로 유발된 c-Fos 단백질 발현에 미치는 영향)

  • 김재효;김풍식;김경식;김민선;박병립;손인철
    • The Journal of Korean Medicine
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    • v.23 no.2
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    • pp.108-118
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    • 2002
  • Objective : Acupuncture and herbal medicine have been used to prevent and treat cerebrovascular accident, such as stroke, and many studies of acupuncture and moxibustion concerning stroke have been undertaken in humans and various animals. However, the protective effect of the electroacupuncture (EA) of Huan-do (GB30) on the transient forebrain ischemia injury has not been published. Methods : The nenroprotective effects of EA (2 ms, 10 Hz, and 1 - 2 mA) of GB30 on the transient forebrain ischemia injury were investigated by immunohistochemistry of c-Fos-like protein in Sprague-Dawley rats. Results : The transient forebrain ischemia injury resulted in increased expression of c-Fos-like protein (cFL) in the dentate gyms (DG) and CAl for 6 hrs after ischemia, and EA increased significantly expression of cFL in the CAl and DG. For 48 hrs after, there was delayed expression of cFL at the CAl and DG, representing the sign of neuronal cell death, but EA decreased the delayed expression of cFL, significantly. Conclusion : These results suggest that the nenroprotective effects of EA on transient forebrain ischemia injury may be related to excitatory regulation of cFL at the early stage and inhibitory regulation in the long term.

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C-FOS EXPRESS10N IN THE RAT TRIGEMINAL SENSORY NUCLEUS COMPLEX FOLLOWING TOOTH MOVEMENT (치아이동에 의한 백서 삼차신경감각핵군내 c-Fos의 발현)

  • Min, Kyung-Ho;Park, Hyo-Sang;Bae, Yong-Chul;Sung, Jae-Hyun
    • The korean journal of orthodontics
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    • v.28 no.3 s.68
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    • pp.441-452
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    • 1998
  • The c-fos is known as neuronal marker of second neurons which is activated by noxious peripheral stimulation. To investigate the changes of c-fos el(pression in the trigeminal nucleus complex during tooth movement, immunohistochemical study was performed. Experimental rats(9 weeks old, 210 gm 21 rats) were divided into seven groups(normal, 1 hour group, 3 hour group, 6 hour group, 12 hour group, 1 day group,3 day group). Rats in the normal group were anesthesized without orthodontic force. Rats in the experimental groups were applied orthodontic force (approximately 30 gm) to upper right maxillary molar. Frozen sections of brain stem were immunostained using rabbit antisera. The changes of c-fos expression were observed with respect to rostrocaudal distribution, laminar organization, md duration of orthodontic force application. The study results were as follows $\cdot$The c-fos nuclei in the dorsal part were observed from ipsilateral transition zone of subnucleus interpolaris and subnucleus caudalis to $C_1$ cervical dorsal horn rostrocaudally. The maximal peak point was the rostral part of subnucleus caudalis. The greatest proportion of c-fos cells were located within lamina I and II. $\cdot$The c-fos nuclei in the dorsal Part were observed from the most caudal part of subnucleus interpolaris to the middle part of the subnucleus caudalis. $\cdot$The number of c-fos immunoreactive dot increased at 1 hour group, reached its maximum at the 3 and 6 hour groups, and showed a decreasing trend after 12 hours. These results imply that nociceptive stimulation caused by continuous orthodontic force might be modulated by transition zone of subnucleus interpolaris and subnucleus caudalis, subnucleus caudalis, $C_1$ spinal dorsal hem.

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Changes of c-Fos Immunoreactivity in Midbrain by Deep Pain and Effects of Aspirin (심부통증이 흰쥐 중뇌에 미치는 c-Fos 면역반응성의 변화와 아스피린의 효과)

  • Jung, Jin A;Yoo, Ki Soo;Hwang, Kyu Keun
    • Clinical and Experimental Pediatrics
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    • v.46 no.7
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    • pp.695-701
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    • 2003
  • Purpose : It had been suggested that pain arising from deep somatic body regions influences neural activity within periaqueductal gray(PAG) of midbrain via distinct spinal pathways. Aspirin is one of the popular non-steroidal anti-inflammatory drugs used in the management of pain. Fos expression was used as a marker for neuronal activity throughout central neurons following painful peripheral stimulation. This study was prepared to investigate changes of c-Fos immunoreactivity in midbrain by deep pain and effects of aspirin. Methods : Male Sprague-Dawley rats were injected with 0.1 mL of 5% formalin in the plantar muscle of the right hindpaw. For experimental group II, aspirin was injected intravenously before injection of formalin. An aspirin-untreated group was utilized as group I. Rats were sacrificed at 0.5, 1, 2, 6 and 24 hours after formalin injection. Rat's brains were removed and sliced in rat brain matrix. Brain slices were coronally sectioned at interaural 1.00-1.36 mm. Serial sections were immunohistochemically reacted with polyclonal c-Fos antibody. The numbers of c-Fos protein immunoreactive neurons in ventrolateral periaqueductal gray(VLPAG) and dorsomedial periaqueductal gray(DMPAG) were counted and analyzed statistically with Mann-Whitney U tests. Results : Higher numbers of c-Fos protein immunoreactive neurons were found in VLPAG. In both VLPAG and DMPAG of formalin-treated group, the numbers of c-Fos protein immunoreactive neurons were significantly higher at all time points than the formalin-untreated group, which peaked at two hours. The numbers of c-Fos immunoreactive neuron of the aspirin-treated group were less compared to the aspirin-untreated group at each time point. Conclusion : These results provide some basic knowledge in understanding the mechanism of formalin-induced deep somatic pain and the effects of aspirin.

Ginsenosides Have a Suppressive Effect on c-Fos Expression in Brain and Reduce Cardiovascular Responses Increased by Noxious Stimulation to the Rat Tooth

  • Jung, Ji-Yeon;Seong, Kyung-Joo;Moon, In-Ohk;Cho, Jin-Hyoung;Kim, Sun-Hun;Kim, Won-Jae
    • The Korean Journal of Physiology and Pharmacology
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    • v.17 no.2
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    • pp.121-125
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    • 2013
  • The purpose of this study is to investigate the antinociceptive effects of ginsenosides on toothache. c-Fos immunoreactive (IR) neurons were examined after noxious intrapulpal stimulation (NS) by intrapulpal injection of 2 M KCl into upper and lower incisor pulps exposed by bone cutter in Sprague Dawley rats. The number of Fos-IR neurons was increased in the trigeminal subnucleus caudalis (Vc) and the transitional region between Vc and subnucleus interpolaris (Vi) by NS to tooth. The intradental NS raised arterial blood pressure (BP) and heart rate (HR). The number of Fos-IR neurons was also enhanced in thalamic ventral posteromedial nucleus (VPMN) and centrolateral nucleus (CLN) by NS to tooth. The intradental NS increased the number of Fos-IR neurons in the nucleus tractus solitarius (NTS) and rostral ventrolateral medulla (RVLM), hypothalamic supraoptic nucleus (SON) and paraventricular nucleus (PVN), central cardiovascular regulation centers. Ginsenosides reduced the number of c-Fos-IR increased by NS to tooth in the trigeminal Vc and thalamic VPMN and CLN. Naloxone, an opioid antagonist, did not block the effect of ginsenoside on the number of Fos-IR neurons enhanced by NS to tooth in the trigeminal Vc and thalamic VPMN and CLN. Ginsenosides ameliorated arterial BP and HR raised by NS to tooth and reduced the number of Fos-IR neurons increased by NS to tooth in the NTS, RVLM, hypothalamic SON, and PVN. These results suggest that ginsenosides have an antinociceptive effect on toothache through non-opioid system and attenuates BP and HR increased by NS to tooth.