• Title/Summary/Keyword: c-Fos

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Influences of Postnatal Ginseng radix Administration on Prenatal Noise Stress-induced c-Fos Expression in the Hippocampus of Offspring Rats (태아 중 소음 스트레스가 출생 후 해마 내 c-Fos 발현에 미치는 인삼의 효과)

  • Jang, Jae-chan;Kim, Youn-Sub;Kim, Ji-Hyouck
    • Journal of Oriental Neuropsychiatry
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    • v.17 no.3
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    • pp.1-10
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    • 2006
  • 목적:임신 중 소음 스트레스가 태아에 어떤 영향을 미치는 가를 관찰하고자, 임신 중인 흰쥐에 소음 스트레스를 가한 후 출생한 새끼 흰쥐에 인삼을 투여하여 신경적 발육에 대한, 특히 새끼 흰쥐 해마의 c-Fos 관점에서 연구하였다. 결과:CIA 영역의 c-Fos-positive 세포의수는, 출생 전 소음 스트레스를 받은 대조군에 비해, 10 mg/kg의 인삼을 투여한 군에서 유의성 있는 감소를 보였다. 해마의 CA2, CA3 영역에서의 c-Fos-positive 세포의 수는 10 mg/kg의 인삼을 투여한 그룹에서, 출생 전 소음 스트레스를 받은 대조군에 비해 유의성 있는 감소를 보였다. 해마의 dentate gyrus 영역에서 c-Fos-positive 세포의 수는, 10 mg/kg의 인삼을 투여한 그룹에서, 출생 전 소음 스트레스를 받은 대조군에 비해 유의성 있는 감소를 보였다. 결론: 인삼은 태아기 소음 스트레스로 야기되는 c-Fos의 변화로 인한 해마의 기능장애에 중화제로서 사용될 수 있다. 그리고 임신 중 소음 스트레스를 경험한 엄마의 아이들에 있어서 정신과적 문제를 치료하는데 유용할 것이다.

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프락토 올리고당과 프락토 올리고당을 함유한 스폰지 케?이 흰쥐의 혈청지질과 장기능 및 단쇄지방산 생성에 미치는 효과

  • 이선영;조정화;이경애
    • Journal of Nutrition and Health
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    • v.36 no.4
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    • pp.344-351
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    • 2003
  • The purpose of this study was to elucidate effects of fructooligosaccharide on gastrointestinal tract and blood lipids of rats when this was supplied as purchased condition or oligosaccharide containing sponge cake. Male Sprague-Dawley rats were assigned to one of 3 treatments 1) control diet 2) 7.5% fructooligosaccharide containing diet (FOS diet) 3) lyophilized sponge cake powder containing diet (FOS-C diet). The sponge cake was made with fractooligosaccharide which replaced 40% of its surose, and the final concentration of fructooligosaccharide in FOS-C diet was 7.5%. Cecal and fecal water contents, amount of cecal content, and cecal wall weight were higher from fructooligosaccharide consumption, whereas total gut transit time was longer in rats consuming fructooligosaccharide compared with those fed control diet. Cecal and fecal pH were lower in FOS and FOS-C groups than in control group. Total cecal SCFA pools were higher from ingesting fructooligosaccharide containing diets compared with control diet. Serum triglyceride levels were lower in rats fed FOS and FOS-C diet than those fed control diet, while serum cholesterol levels were unaffected by treatment. Therefore the effects of fructooligosaccharide in sponge cake on serum lipids and gastrointestinal tract were similar to those of intact fructooligosaccharide. Also, adding 7.5% of FOS accompanied diarrhea symptom which suggests some precaution are needed when using FOS.

Regulation of c-Fos and c-Jun Gene Expression by Lipopolysaccharide and Cytokines in Primary Cultured Astrocytes: Effect of PKA and PKC Pathways

  • Suh Hong-Won;Choi Seong-Soo;Lee Jin-Koo;Lee Han-Kyu;Han Eun-Jung;Lee Jongho
    • Archives of Pharmacal Research
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    • v.27 no.4
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    • pp.396-401
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    • 2004
  • The effects of lipopolysaccharide (LPS) and several cytokines or the c-fos and c-jun mRNA expression were examined in primary cultured astrocytes. Either LPS (500 ng/mL) or inter-feron-$\gamma$ (IFN-$\gamma$ 5 ng/mL) alone increased the level of c-fos mRNA (1 h). However, tumor necro-sis factor-$\alpha$ (TNF-$\alpha$; 10 ng/mL) or interleukin-4 (IL-1$\beta$: 5 ng/mL) alone showed no significant induction of the level of c-fos mRNA. TNF-$\alpha$ showed a potentiating effect in the regulation of LPS-induced c-fos mRNA expression, whereas LPS showed an inhibitory action against IFN-Y-induced c-fos mRNA expression. LPS, but not TNF-$\alpha$, IL-1$\beta$ and IFN-$\gamma$, increased the level of c-jun mRNA (1 h). TNF-$\alpha$ and IFN-$\gamma$ showed an inhibitory action against LPS-induced c-jun mRNA expression. Both phorbol 12-myristate 13-acetate (PMA; 2.5 mM) and forskolin (FSK, 5 mM) increased the c-fos and c-jun mRNA expressions. In addition, the level of c-fos mRNA was expressed in an antagonistic manner when LPS was combined with PMA. When LPS was co-treated with either PMA or FSK, it showed an additive interaction for the induction of c-jun mRNA expression. Our results suggest that LPS and cytokines may be actively involved in the regulation of c-fos and c-jun mRNA expressions in primary cultured astrocytes. Moreover, both the PKA and PKC pathways may regulate the LPS-induced c-fos and c-jun mRNA expressions in different ways.

Effects of Microcurrent Stimulation on c-fos and Calcitonin Gene-Related Peptide Expression in the Spinal Cord on Rats Induced Pain (통증유발 백서에서 미세전류자극이 척수 분절 내 c-fos 및 CGRP 발현에 미치는 영향)

  • Kim, Gye-Yeop;Jeong, Hyun-Woo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.19 no.1
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    • pp.75-80
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    • 2005
  • The purpose of study is that we will observe the change of c-fos and CGRP with the immunohistochemistry method and then we will study the effect of microcurrent stimulation following the frequency after inducing pain to rats with capsaicin. The experimental groups were divided by microcurrent application and pain induce. Normal control groups is used in experiment I, the group which we induce pain is used in experiment II, the application group which we induce pain and then the high frequency microcurrent stimulation is used in experiment III, the application group which we induce pain and then the low frequency microcurrent stimulation is used in experiment IV. c-fos was strongly expressed after pain induced 2 hours and positive neurons were decreased from 2 hours. At 7 days, positive neuron recovers to normal range, But c-fos positive neuron of microcurrent stimulation group were decreased from 2 hours. CGRP was strongly expressed after pain induced 24 hours, and positive neurons were decreased from 7 days. These results suggests that microcurrent stimulation therapy effect to control pain according to expression of c-fos and CGRP examined by immunohistochemistry. Also high frequency microcurrent stimulation is more effective than low frequency microcurrent stimulation for controling the pain.

Acupuncture on ST36 Increases c-Fos Expression in vlPAG of Visceral Pain-induced Mice

  • Choo, Jin-Suk;Song, Yun-Kyung;Lim, Hyung-Ho
    • The Journal of Korean Medicine
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    • v.29 no.1
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    • pp.39-46
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    • 2008
  • Background : Acupuncture has been used as a clinical treatment in Oriental medicine for various diseases including pain relief. The descending pain control system of periaqueductal gray (PAG) is a powerful pain control system in mammalians. Expression of c-Fos is used as a marker for stimuli-induced metabolic changes of neurons. Objective : In the present study, the effects of acupuncture on analgesic effect in visceral pain were investigated through the writhing reflex and c-Fos expression in ventrolateral PAG (vlPAG) area using immunohistochemistry in mice. Method : For the writhing test, mice were divided into five groups. Immediately after finishing the behavioral test, the animals were weighed and overdosed with Zoletil. After a complete lack of response was observed, the brains of the mice were dissected into serial coronal sections, and c-Fos immunohistochemistry was performed. Statistical analysis of all data was performed using one-way ANOVA. Result : The present results showed that acupuncture affected the writhing reflex and that Choksamni (zusnali) acupoint and aspirin significantlysuppressed acetic acid treatment-induced increased writhing reflex, and the expression of c-Fos in vlPAG was significantly increased in the acupunctured group. Conclusion : The present study suggests that acupuncture has an antinociceptive effect on acetic acid-induced visceral pain by increase of c-Fos expression in mice. Aspirin also showed analgesic effect, however the mechanism is different from the acupuncture.

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Effects of Moutan Cortex on Corticotropin-Releasing Factor, c-Fos and Tyrosine Hydroxylase in Forced Swimming Test (목단피(牧丹皮)가 강제수영부하시험에서 Corticotropin-Releasing Factor, c-Fos 와 Tyrosine Hydroxylase에 미치는 영향)

  • Sung, Young-Suk;Lee, Tae-Hee
    • Herbal Formula Science
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    • v.17 no.1
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    • pp.129-140
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    • 2009
  • Objectives : The goal of this study was to investigate the effect of Moutan Cortex as antidepressant in forced swimming test(FST) model. Methods : The expressions of corticotropin-releasing Factor(CRF), c-Fos and tyrosine hydroxylase(TH) were measured with immunohistochemical method at paraventricular nucleus (PVN), locus coeruleus(LC) and ventral tegmental area(VTA). Results : The duration of immobility in the forced swimming test was significantly decreased in the Moutan Cortex 100 mg/kg treated group in comparison with the control group(p<0.01). CRF and c-Fos expressions at PVN were decreased in the Moutan Cortex 100mg/kg treated group in comparison with the control group. But only the expression of c-Fos was shown the significance(p<0.05). TH expressions at LC and VTA were significantly decreased in the Moutan Cortex 100mg/kg and 400mg/kg treated group in comparison with the control group(p<0.001). Conclusion : According to the results, Moutan Cortex has the antidepressant effect by showing the reduced immobility through the reduction of c-Fos expression at PVN and the reduction of TH expression at LC and VTA.

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Expression of spinal cord c-fos with cold therapy in rats of carrageenan-induced inflammatory muscle pain (Carrageenan으로 유도된 염증성 근통증 흰쥐 모델에서 냉치료에 의한 척수의 c-fos의 발현)

  • Paek Yun-Woong
    • The Journal of Korean Physical Therapy
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    • v.15 no.4
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    • pp.190-198
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    • 2003
  • Expression of c-fos, an immediate early gene, has accepted to be a marker of functional activity in neurons. This study was aimed to investigate the effects of cold therapy on the expression of spinal cord c-fos in rats of carrageenan-induced muscle pain. Muscle pain was induced in male Sprague-Dawley rats by intra-muscular injection of gastrocnemius with $2\%$ carrageenan. The paw withdrawal latency (PWL) and tail flick test (TFT) responses to heat stimuli were used to detect secondary hyperalgesia produced by the muscle pain and measured to assess the effects of cold. The expression of c-fos was determined in the lumbar regions of the spinal cord by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry assays. The secondary hyperalgesia to heat simuli (PWL and TFT) were significantly reduced in cold therapy compared with that in the controls. In RT-PCR assays the expression of c-fos mRNA was down-regulated in the lumbar spinal cord in cold group. In addition, Fos immunoreactivity in the dorsal horn of the lumbar spinal cord was decreased in cold group. These results suggested that application of cold attributed to increase PWL and TFT responses and to decrease expression of the c-fos produced by muscle pain.

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Methanolic extract from the root of Bupleuri falcatum L. attenuates cocaine-induced c-Fos expression in rat brain. (시호 메탄올 추출물이 코카인 약물중독에 의한 흰쥐 뇌의 c-Fos 발현에 미치는 영향)

  • Choi, Seong-Hun;Ku, Sae-Kwang;Han, Chang-Hyun;Yang, Chae-Ha
    • Korean Journal of Oriental Medicine
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    • v.16 no.1
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    • pp.141-147
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    • 2010
  • Objectives : The aim of present study is to examine the effect of methanolic extract from the root of Bupleurum falcatum L. (BF) on acute cocaine-induced c-Fos expression in the rat caudate putamen (CPu), a major dopaminergic terminal. Methods : Male Sprague-Dawley rats were subjected to an intraperitoneal injection with either cocaine hydrochloride (20 mg/kg) or saline 30 min after an administration of either extract of BF (100 mg/kg, i.p.) or vehicle. Animals were sacrificed 2 hr after treatment with cocaine or saline for immunohistochemistry. Quantification of brain slices was examined for c-Fos positive nuclei using light microscopy. Results : Pretreatment with BF significantly attenuated cocaine-induced c-Fos expression in the rat CPu. Conclusions : This finding suggests that BF has the inhibitory effect on cocaine-induced c-Fos expression in the rat CPu via possibly modulating the activities of central dopaminergic systems.

Swimming During Pregnancy Increases the Expression c-Fos and c-Jun in the Hippocampus of Rat Offspring

  • Sim, Young-Je;Kim, Jee-Youn;Kim, Chang-Ju
    • Korean Journal of Exercise Nutrition
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    • v.13 no.1
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    • pp.23-28
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    • 2009
  • The expression of c-Fos and c-Jun represents neuronal activity and plays a crucial role in the shaping of the development of brain. During the late pregnancy, exercise is known to influence neuronal activity of offspring. In the present study, the effect of swimming during pregnancy on the expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of rat offspring was investigated using immunohistochemistry. Pregnant rats in the swimming group were forced to swim for 10 min once a day from 15 days after pregnancy until delivery. The expression of c-Fos and c-Jun in the CA1, CA2, CA3 regions, and the dentate gyrus of the hippocampus of pups was significantly increased by maternal swimming during late pregnant period. The present results show that prenatal swimming may enhance the neuronal activity of pups and affect the neonatal brain development.

Effects of Polygala tenuipolia on Expression of Fos-protein and Ethanol Amnesia in Rat

  • Lee, Soon-Chul;Kim, Kwang-Kyu;Jang, Jin-Hee;You, Kwan-Hee
    • Biomedical Science Letters
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    • v.8 no.3
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    • pp.167-172
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    • 2002
  • Effect of single administration of Polygala tenuipolia was examined on short-term memory in step through test and the intensity of the immunoreactive c-Fos protein induced by oral administration of ethanol. The acquisition of memory was significantly reduced by ethanol, and ethanol amnesia was remarkably reversed following oral administration of Polygala tenuifolia. c-Fos protein in normal rat brain was highly expressed in order of thalamus, pariental cortex, hippocampus, hypothalamus, amygdaloid and cingulate cortex. The expression of Fos protein was remarkably suppressed by single administration of ethanol. The inhibitory effect of ethanol on expression of Fos protein was reversed by single administration of Polygara tenuipolia, especially tissues of limbic areas such as amygdala, parietal cortex and CA3 of hippocampus. These results suggested that the amelioration process of Polygala tenuipolia on ethanol amnesia seems to be involve the expression of c-Fos protein in partly.

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