• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.647-652
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• 1994

This study was carried out to investigate the actual condition of canine herpesvirus(CHV) infection in Korea. A total of 338 serum samples were collected randomly from the breeding and companion dogs in the local areas in Korea. The serum samples were used to determine the actual condition of the canine herpesvirus infection in Korea using enzyme linked immunosorbent assay(ELISA). The mean prevalence of CHV infection in dogs was 37% and that of the breeding and companion dogs was 58% and 28%, respectively. The prevalence of CHV infection in Seoul, Kyung-gi, Chung Nam, Cheon Nam and Pusan was detected 23%, 28%, 18%, 28% and 70%, respectively. The prevalence of CHV infection in less and more than 6 months old dogs, and in male and female dogs was 26% and 40%, 42% and 33%, respectively. These results indicate that the incidence of CHV infection is high in Korea, especially breeding dogs and older dogs.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.569-581
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• 1994

This study was carried out to investigate the pathogenesis of canine herpesvirus(CHV) infection in dogs. The 17 puppies, one day old, delivered from CHV seronegative 3 dams were divided into two groups. The 13 puppies were inoculated intranasally with 1ml of CHV-KK inoculum($5{\times}10^{5.6}TCID_{50}/ml$) and 4 puppies were served as control. And then the puppies were sacrificed at 2, 4, 6 and 7 days after the treatment, and collected blood, nasal mucosa, trigeminal nerve, trigeminal ganglion, bone marrow, eye, brain and other major organs. These organs were examined histopathologically and electron microscopically. The platelets of puppies infected with CHV were dramatically decreased because of the damages of vascular endothelial cells. Histopathologically, necrotizing vasculitis and neuritis were proceeded the generalized focal necrosis of all organs. Necrotic changes in trigeminal ganglion, trigeminal nerve and ventroposteriomedial nucleus of thalamus were observed in 4 puppies infected with CHV. Herpesviral particles, various forms of maturation, were observed in endothelial cells of the alveolar capillary and hepatic sinusoid with electron microscopy. These results suggest that the generalized focal necrcsis of all organs including brain and eyes in canine herpesvirus infection were resulted from generalized vasculitis, and also the hemonecrotizing meningoencephalitis was related to the necrosis of trigeminal nerve pathway.

• Korean Journal of Veterinary Research
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• v.34 no.3
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• pp.583-591
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• 1994

This study was carried out to investigate the pathogenesis of canine herpesvirus(CHV) infection in dogs. The 17 puppies, one day old, delivered from CHV seronegative 3 dams were divided into two groups. The 13 puppies were inoculated intranasally with 1ml of CHV-KK inoculum($5{\times}10^{5.6}TCID_{50}/ml$) and 4 puppies were served as control. And then the puppies were sacrificed at 2, 4, 6 and 7 days after the treatment, and sampled nasal mucosa, trigeminal nerve, trigeminal ganglion, bone marrow, eye, brain and other major organs for the immunohistochemical examination. Distribution of CHV antigens was limited in cytoplasms and nuclei of necrotic nasal epthelia at 2 days after infection. At 4 days after infection, CHV antigens were detected in vascular walls and peripheral nerves of nasal lamina propria, reticuloendothelial cells of spleen, interstitium of kidney, leptomeningeal vascular walls and alveolar walls, At 6 and 7 days after infection, CHV antigens were detected in all of the necrotic area. CHV antigens were also detected in vascular endothelial cells of various organs and in blood leukocytes from 4 days after infection. Among the six puppies in which necrotic lesions of central nervous system were observed, CHV antigens were detected in trigeminal ganglion, trigeminal nerve and ventroposteriomedial nucleus of four puppies and in spinal trigeminal nucleus of three puppies. These results indicate that the generalized focal necrosis of all organs including brain and eyes in canine herpesvirus infection were resulted from generalized vasculitis with leukocyte-associated viremia, and also the hemonecrotizing meningoencephalitis was resulted from spreading of CHV via blood and nerve trunk.

• Korean Journal of Veterinary Research
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• v.32 no.2
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• pp.217-225
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• 1992

Five puppies, 14 days old in the same litter showed acute symptoms such as painful crying, anorexia, abdominal pain and depression with fatal terminations. Necropsy of a puppy revealed hemorrhagic and necrotic foci in the lungs and kidney. The histological lesions were characterized with fibro-necrotizing foci in the lungs, nonsuppurative encephalitis and intranuclear inclusions of liver cells. A cytopathogenic agent was isolated from the thoracic fluid of a dead puppy by cell culture with primary dog kidney cells. The puppies inoculated with the field isolate showed the same clinical signs and lesions as those of the spontaneous cases. Viral particles were observed in suspension of the isolated agent by electron microscopy. The primary dog kidney cells infected with the field isolate showed fluorescent foci against anti-CHV monoclonal antibody after FA stain. On these findings of the disease it was diagnosed as CHV infection. The report signifies the first description of an epizootic of CHV infection in Korea.