• Title/Summary/Keyword: chitin. Solenocera prominentis

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Isolation and Purification of Chitin from Shrimp Shells by Protease Pretreatment (Protease의 전처리에 의한 새우껍질로부터 키틴의 분리와 정제)

  • Ryu, Beung-Ho;Lee, Sang-Hoon
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.6-10
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    • 1995
  • Chitin was prepared from Solenocera prominentis by deproteinization pretreatment of Neutrase. The optimal enzyme concentration of neutrase, pH, and temperature on deproteinization were 3.0 mg/ml, pH 6.0 and $50^{\circ}C$ as indicated by the minimum protein remaining on the chitin. The residual protein, the degree of deacetylation, Ca and P content in chitin prepared from Solenocera prominentis were similar with commercial chitin. The molecular weight was $1.2{\times}10^{8}$ dalton and the yield of chitin was 25.8%.

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Utilization of Chitin Prepared from the Shellfish Crust 1. Functional Properties of Chitin, Chitosan, and Microcrystalline Chitin (갑각류부산물을 이용한 키틴의 제조 및 이용에 관한 연구 1. 키틴, 키토산 및 미세결정화 키틴의 기능특성)

  • AHN Chang-Bum;LEE Eung-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.25 no.1
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    • pp.45-50
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    • 1992
  • To utilize shellfish by-products effectively, chitin, chitosan, and microcrystalline chitin were prepared from 6 kinds of crustacean shells(Antarctic krill, Euphausia superba; Red snow crab, Chinonecetes japonicus: Daelongsuyum shrimp, Solenocera prominentis: Lobster, Linuparus trigonus: Gasibal shrimp, Nephrops thomsoni: Blue crab, Portunus trituberculatus) and their functional properties were studied. Apparent volume(AV), settling volume(SV), water binding capacity(WBC), and fat binding capacity(FBC) of various chitins, chitosans, and microcrystalline chitins ranged from $3.1\pm0.1ml/g\;to\;27.0\pm0.2ml/g$ from $5.1\pm0.1ml/g\;to\;45.0\pm0.2ml/g,\;from\;318\pm40g/100g\;to\;2,382\pm12g/100g,\;and\;from\;235\pm20g/100g\;to\;2,169\pm20g/100g$, respectively, and the krill chitin and chitosan had the highest AV, SV, WBC, and FBC of them. Chitins and chitosans did not produce emulsion but microcrystalline chitins showed emulsifying properties. Emulsifying capacity and stability of various microcrystalline chitins ranged from $18.2\pm4.0\%\;to\;50.1\pm2.5\%\;and\;from\;15.2\pm3.5\%\;to\;31.1\pm1.0\%$, respectively. Dye binding capacity of microcrystalline chitins was higher than that of chitins or chitosans.

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