• Title/Summary/Keyword: co-localization

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Visualization of Multicolored in vivo Organelle Markers for Co-Localization Studies in Oryza sativa

  • Dangol, Sarmina;Singh, Raksha;Chen, Yafei;Jwa, Nam-Soo
    • Molecules and Cells
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    • v.40 no.11
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    • pp.828-836
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    • 2017
  • Eukaryotic cells consist of a complex network of thousands of proteins present in different organelles where organelle-specific cellular processes occur. Identification of the subcellular localization of a protein is important for understanding its potential biochemical functions. In the post-genomic era, localization of unknown proteins is achieved using multiple tools including a fluorescent-tagged protein approach. Several fluorescent-tagged protein organelle markers have been introduced into dicot plants, but its use is still limited in monocot plants. Here, we generated a set of multicolored organelle markers (fluorescent-tagged proteins) based on well-established targeting sequences. We used a series of pGWBs binary vectors to ameliorate localization and co-localization experiments using monocot plants. We constructed different fluorescent-tagged markers to visualize rice cell organelles, i.e., nucleus, plastids, mitochondria, peroxisomes, golgi body, endoplasmic reticulum, plasma membrane, and tonoplast, with four different fluorescent proteins (FPs) (G3GFP, mRFP, YFP, and CFP). Visualization of FP-tagged markers in their respective compartments has been reported for dicot and monocot plants. The comparative localization of the nucleus marker with a nucleus localizing sequence, and the similar, characteristic morphology of mCherry-tagged Arabidopsis organelle markers and our generated organelle markers in onion cells, provide further evidence for the correct subcellular localization of the Oryza sativa (rice) organelle marker. The set of eight different rice organelle markers with four different FPs provides a valuable resource for determining the subcellular localization of newly identified proteins, conducting co-localization assays, and generating stable transgenic localization in monocot plants.

An Implementation of UWB IR System for Long Distance and High-precision Localization (장거리 고정밀 측위를 위한 UWB IR 시스템 구현)

  • Kim, Ki-Yun;Kim, Gil-Gyeom;Kim, Tae-Kwon
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.30 no.1
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    • pp.87-95
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    • 2016
  • Recently, the interests of the precise localization are rapidly increasing, which are linked to IoT(Internet of Things) sensors. The precise localization in indoor environment can be utilized in navigation, security, anti-collision, and various location based services etc. However, conventional positioning sensors, such as PIR, ultrasonic, microwave etc. are vulnerable to weather or insensitive to direction of subject movement or low precision performance. In this paper we implement a UWB-IR localization system for long distance and high-precision localization, which is not affected by temperature, light and weather. The proposed system was divided and designed by H/W, Antenna, S/W parts, each of which was designed based on an accurate analysis and simulation. As a result, we can implemented and verified UWB IR system with precise localization performance.

Neuronal Nitric Oxide Synthase-Immunoreactive Neurons In the Hamster Visual Cortex: Lack of Co-localization with Parvalbumin (햄스터 시각피질에서 Neuronal nitric oxide synthase-면역반응성 뉴런: parvalbumin과의 co-localization 부재)

  • Jin Mi-Joo;Lee Jee-Eun;Ye Eun-Ah;Jeon Chang-Jin
    • Journal of Life Science
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    • v.15 no.3 s.70
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    • pp.344-351
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    • 2005
  • Nitric oxide (NO) and calcium-binding proteins occur in various types of cells in the central nervous system. They are important signaling and calcium buffering molecules, respectively. In the present study, using immunocytochemistry we examined the distribution and the co-localization pattern of neurons containing neuronal nitric oxide synthase (nNOS) and parvalbumin in the visual cortex of hamster. The overall number of parvalbumin-immunoreactive (IR) neurons was 17 times higher than that of the nNOS-IR neurons in the hamster visual cortex. The highest differences were found in layer V, where parvalbumin-IR neurons were 54.7 times more abundant than nNOS-IR neurons. Many nNOS- and parvalbumin-IR neurons were similar in size, shape, and manner of distribution in the visual cortex. However, two-color immunofluorescence revealed that no neurons in the hamster visual cortex expressed both nNOS and parvalbumin. The present results indicate that there are subtle species differences in the co-localization pattern between nNOS and calcium-binding proteins. The present results also suggest not only the heterogeneity and functional diversity of nNOS-IRneurons in the visual cortex, but also the importance of understanding animal diversity

A Study on plans for improving localization of process pumps for petrochemical plants (석유화학 플랜트용 프로세스 펌프의 국산화율 제고 방안에 관한 연구)

  • Cho, Won-Bae;Moon, Seung-Jae;Yoo, Hoseon
    • Plant Journal
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    • v.5 no.3
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    • pp.50-58
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    • 2009
  • In this paper, the present condition for localization of process pumps and the enhancement method of the localization ratio of process pumps for refinery and chemical plant market were studied. The market of plant industry in the world has grown rapidly since 2000. However, the profit of domestic plant EPC compaies cound not have been increased as much as the market scale because they procured most of equipment from overseas. To make remarkable profit of plant EPC companies in the petrochemical industry, localization of equipments is required. Suitable equipment for localization is process pump applied API 610 standard. An purchased amount of pumps from overseas by domestic plant EPC companies in the last two years were 230 billion won. If process pumps are localized then an profit of plant EPC project will increase.

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Localization of Mobile Robot Using Color Landmark mounted on Ceiling (천장 부착 컬러 표식을 이용한 이동로봇의 자기위치추정)

  • Oh, Jong-Kyu;Lee, Chan-Ho
    • Proceedings of the KIEE Conference
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    • 2001.11c
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    • pp.91-94
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    • 2001
  • In this paper, we proposed localization method of mobile robot using color landmark mounted on ceiling. This work is composed 2 parts : landmark recognition part which finds the position of multiple landmarks in image and identifies them and absolute position estimation part which estimates the location and orientation of mobile robot in indoor environment. In landmark recognition part, mobile robot detects artificial color landmarks using simple histogram intersection method in rg color space which is insensitive to the change of illumination. Then absolute position estimation part calculates relative position of the mobile robot to the detected landmarks. For the verification of proposed algorithm, ceiling-orientated camera was installed on a mobile robot and performance of localization was examined by designed artificial color landmarks. As the result of test, mobile robot could achieve the reliable landmark detection and accurately estimate the position of mobile robot in indoor environment.

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Mitochondrial Location of Severe Acute Respiratory Syndrome Coronavirus 3b Protein

  • Yuan, Xiaoling;Shan, Yajun;Yao, Zhenyu;Li, Jianyong;Zhao, Zhenhu;Chen, Jiapei;Cong, Yuwen
    • Molecules and Cells
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    • v.21 no.2
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    • pp.186-191
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    • 2006
  • Severe acute respiratory syndrome-associated coronavirus (SARS-CoV), a distant member of the Group 2 coronaviruses, has recently been identified as the etiological agent of severe acute respiratory syndrome (SARS). The genome of SARS-CoV contains four structural genes that are homologous to genes found in other coronaviruses, as well as six subgroup-specific open reading frames (ORFs). ORF3 encodes a predicted 154-amino-acid protein that lacks similarity to any known protein, and is designated 3b in this article. We reported previously that SARS-CoV 3b is predominantly localized in the nucleolus, and induces G0/G1 arrest and apoptosis in transfected cells. In this study, we show that SARS-CoV 3b fused with EGFP at its N- or C- terminus co-localized with a mitochondriaspecific marker in some transfected cells. Mutation analysis of SARS-CoV 3b revealed that the domain spanning amino acids 80 to 138 was essential for its mitochondria localization. These results provide new directions for studies of the role of SARS-CoV 3b protein in SARS pathogenesis.

Self Localization of Mobile Robot Using Sonar Sensing and Map Building

  • Kim, Ji-Min;Lee, Ki-Seong;Jeong, Tae-Won
    • 제어로봇시스템학회:학술대회논문집
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    • 2004.08a
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    • pp.1931-1935
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    • 2004
  • A location estimate problem is critical issues for mobile robot. Because it is basic problem in practical use of the mobile robot which do what, or move where, or reach an aim. Already there are many technologies of robot localization (like GPS, vision, sonar sensor, etc) used on development. But the elevation of accurateness was brought the problem that must consider an increase of a hardware cost and addition electric power in each ways. There is the core in question to develop available and accurate sensing algorithm though it is economical. We used a ultrasonic sensor and was going to implement comparatively accurate localization though economical. Using a sensing data, we could make a grid map and estimate a position of a mobile robot. In this paper, to get a satisfactory answer about this problem using a ultrasonic sensor.

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Sound localization for Teller Following of A dialog type Humanoid Robot (대화형 로봇의 화자 추종을 위한 sound localization)

  • Shim, H.M.;Lee, J.S.;Kwon, O.S.;Lee, E.H.;Hong, S.H.
    • Proceedings of the KIEE Conference
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    • 2001.11c
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    • pp.111-114
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    • 2001
  • In this paper, we supposed teller following algorithm that using sound localization for developing dialog type humanoid robot. A sound localization is studied for develop the techniques of an efficient 3-D sound system based on the psychoacoustics of spatial hearing with multimedia or virtual reality. When a robot talk with human, it is necessary that robot follow human for improved human interface and adaptive noise canceling. We apply this algorithm to robot system.

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