• Title/Summary/Keyword: confocal microscopy

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Solid-immersion lens based confocal microscopy using super-continuum generation effect (Super-continuum generation 현상을 이용한 Solid-immersion lens 기반 공초점 현미경)

  • Lee, Won-Sup;Moon, Hyungbae;Lim, Geon;Choi, Guk-Jong;Park, No-Cheol
    • Transactions of the Society of Information Storage Systems
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    • v.11 no.2
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    • pp.22-25
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    • 2015
  • In this paper, we demonstrate solid-immersion lens based confocal microscopy using super-continuum generation effect. Using super-continuum generation effect, we could diversify the excitation wavelength of confocal microscopy. Further, high refractive index of solid-immersion lens would increase the resolution of confocal microscopy. As a result, by applying the super-continuum generation effect and solid-immersion lens to confocal microscopy, some problems of confocal fluorescent microscopy, the excitation wavelength and the resolution, could be overcome. To verify it, we made home-built solid-immersion lens based confocal microscopy using super-continuum generation effect, and evaluate the performance of the system.

Confocal Microscopy of Colloidal Suspensions

  • Kim, Jin Young;Weon, Byung Mook
    • Applied Microscopy
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    • v.44 no.1
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    • pp.30-33
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    • 2014
  • Colloidal systems or colloids consist of microparticles or nanoparticles (solute) uniformly suspended in a liquid (solvent), also called colloidal suspensions. They can mimic and exhibit microscopic or atomic aspects of molecular and atomic systems. They have been increasingly studied because of their similarity with atomic systems. They can be microscopically observed by optical microscopes because they are large enough in size and slow in motion to be monitored; microscopic methods are very useful and powerful in research on colloidal systems. Recently, confocal laser microscopy has been known as a powerful tool to obtain information of real-space and real-time behaviors of colloidal suspensions. In particular, it is possible to exactly track individual colloids in three dimensions with confocal microscopy. In this article, we briefly discuss the usefulness of confocal microscopy in colloidal systems that are currently used as model systems to resolve important questions in materials science.

Measurement of metal materials structure by using the manufactured Scanning Confocal Microscopy (초소형 공초점 현미경의 제작과 금속의 구조 측정)

  • Seo, Myeong-Hee;Kim, Jong-Bae;Kwon, Nam-Ic
    • Journal of the Korean Society for Precision Engineering
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    • v.25 no.11
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    • pp.52-57
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    • 2008
  • We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.

Intracellular Trafficking of Transferrin-Conjugated Liposome/DNA Complexes by Confocal Microscopy

  • Lee Sang Mi;Kim Jin-Seok
    • Archives of Pharmacal Research
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    • v.28 no.1
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    • pp.93-99
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    • 2005
  • Intracellular trafficking of transferrin-conjugated dimethyldioctadecyl-ammonium bromide liposome $(T_f-liposome)/DNA$ complexes in HeLa cells was studied using the double-labeled fluorescence technique and confocal microscopy. The size of the $T_f-liposome/DNA$ complex was about 367 nm in diameter and the zeta-potential of it at a 5:1 (w/w) ratio was almost neutral. The intracellular pathway of the $T_f-liposome/DNA$ complex, noted as green (FITC), red (rhodamine) or yellow (FITC + rhodamine) fluorescence, was elucidated from the plasma membrane to the endosome (or lysosome), and finally to the nucleus. The results of this study indicate that plasmid DNA enters into the nucleus not only as a free form but as an associated form complexed with $T_f-liposome$. More interestingly, the $T_f-liposome$ undergoes a nuclear location in the form of ordered structures. This could be a very useful piece of information in designing a safe and advanced gene delivery system.

New Measurement of Whitening Effects by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 신규 미백 효과 측정 연구)

  • Kim, Myong Ki;Cho, Seok-Cheol;Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.279-285
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    • 2015
  • Hyperpigmentation on face is a highly anxiety-producing symptom, especially for women from the aspect of beauty. Pigmentation of the skin is related to the amount of melanin that provides protection against UV radiation. In vivo reflectance confocal microscopy is a non-invasive imaging tool allowing visualization of the skin without tissue alteration, by placing a microscopy directly on the living skin. The aim of this study was to develop the new evaluation method of whitening effects using in vivo reflectance confocal microscopy and to validate other instruments for measuring skin colors, and UV-induced hyperpigmentation was elicited on the inside skin of the forearm. It suggested that the new method for whitening effects using the confocal microscopy was useful to evaluate the de-pigmentation products and to easy for understanding to customers.

Confocal Microscopy Image Segmentation and Extracting Structural Information for Morphological Change Analysis of Dendritic Spine (수상돌기 소극체의 형태변화 분석을 위한 공초점현미경 영상 분할 및 구조추출)

  • Son, Jeany;Kim, Min-Jeong;Kim, Myoung-Hee
    • Journal of the Korea Society for Simulation
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    • v.17 no.4
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    • pp.167-174
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    • 2008
  • The introduction of confocal microscopy makes it possible to observe the structural change of live neuronal cell. Neuro-degenerative disease, such as Alzheimer;s and Parkinson’s diseases are especially related to the morphological change of dendrite spine. That’s the reason for the study of segmentation and extraction from confocal microscope image. The difficulty comes from uneven intensity distribution and blurred boundary. Therefore, the image processing technique which can overcome these problems and extract the structural information should be suggested. In this paper, we propose robust structural information extracting technique with confocal microscopy images of dendrite in brain neurons. First, we apply the nonlinear diffusion filtering that enhance the boundary recognition. Second, we segment region of interest using iterative threshold selection. Third, we perform skeletonization based on Fast Marching Method that extracts centerline and boundary for analysing segmented structure. The result of the proposed method has been less sensitive to noise and has not been affected by rough boundary condition. Using this method shows more accurate and objective results.

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