• 제목/요약/키워드: confocal microscopy

검색결과 436건 처리시간 0.026초

상아세관의 주행방향에 따른 상아질 접착제의 침투양상에 대한 공초점레이저주사현미경 연구 (EFFECT OF DENTINAL TUBULES ORIENTATION ON PENETRATION PATTERN OF DENTIN ADHESIVES USING CONFOCAL LASER SCANNING MICROSCOPY)

  • 김동준;황윤찬;김선호;오원만;황인남
    • Restorative Dentistry and Endodontics
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    • 제28권5호
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    • pp.392-401
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    • 2003
  • The purpose of this study was to evaluate the penetration pattern of dentin adhesives according to the orientation of dentinal tubules with confocal laser scanning microscopy. Specimens having perpendicular. parallel and oblique surface to dentinal tubules were fabricated. The primer of dentin adhesives (ALL $BOND^{\circledR}{\;}2,{\;}CLEARFIL^{TM}$ SE BOND and PQ1) was mixed with fluorescent material. rhodamine B isothio-cyanate (Aldrich Cherm. CO., Milw., USA), It was applied to the specimens according to the instructions of manufactures. The specimens were covered with composite resin (Estelite, shade A2) and then cut to a thickness of 500$\mu\textrm{m}$ with low speed saw (Isomet^{TM}, Buehler. USA). The adhesive pattern of dentin adhesives were observed by fluorescence image using confocal laser scanning microscopy. The results were as follows. 1. For the groups with tubules perpendicular to bonded surface. funnel shape of resin tag was observed in all specimen. However. resin tags were more prominent in phosphoric acid etching system (ALL $BOND^{\circledR}$ 2 and PQ1) than self etching system ($CLEARFIL^{TM}$ SE BOND). 2. For the groups with tubules parallel to bonded surface. rhodamine-labeled primer penetrated into peritubular dentin parallel to the orientation of dentinal tubules. But rhodamine-labeled primer of PQ1 diffused more radially into surrounding intertubular dentin than other dentin adhesive systems. 3. For the groups with tubules oblique to bonded surface. resin tags appeared irregular and discontinuous. But they penetrated deeper into dentinal tubules than other groups.

Confocal Microscopy Measurement of the Fiber Orientation in Short Fiber Reinforced Plastics

  • Lee, Kwang Seok;Lee, Seok Won;Youn, Jae Ryoun;Kang, Tae Jin;Chung, Kwansoo
    • Fibers and Polymers
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    • 제2권1호
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    • pp.163-172
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    • 2001
  • To determine three-dimensional fiber orientation states in injection-molded short fiber composites a CLSM (Confocal Laser Scanning Microscope) is used. Since the CLSM optically sections the composites, more than two cross-sections either on or below the surface of the composite can be obtained. Three dimensional fiber orientation states can be determined with geometric parameters of fibers on two parallel cross-sections. For experiment, carbon fiber reinforced polystyrene is examined by the CLSM. Geometric parameters of fibers are measured by image analysis. In order to compactly describe fiber orientation states, orientation tensors are used. Orientation tensors are determined at different positions of the prepared specimen. Three dimensional orientation states are obtained without the difficulty in determining the out-of-plane angles by utilizing images on two parallel planes acquired by the CLSM. Orientation states are different at different positions and show the shell-core structure along the thickness of the specimen.

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Single-molecule Detection of Fluorescence Resonance Energy Transfer Using Confocal Microscopy

  • Kim, Sung-Hyun;Choi, Don-Seong;Kim, Do-Seok
    • Journal of the Optical Society of Korea
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    • 제12권2호
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    • pp.107-111
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    • 2008
  • We demonstrated single-molecule fluorescence resonance energy transfer (FRET) from single donor-acceptor dye pair attached to a DNA with a setup based on a confocal microscope. Singlestrand DNAs were immobilized on a glass surface with suitable inter-dye distance. Energy transfer efficiency between the donor and the acceptor dyes attached to the DNA was measured with different lengths of DNA. Photobleaching of single dye molecule was observed and used as a sign of single-molecule detection. We could achieve high enough signal-to-noise ratio to detect the fluorescence from a single-molecule, which allows real-time observation of the distance change between single dye pairs in nanometer scale.

3차원 Confocal Imaging과 생체 영상 분석 (Three Dimensional Confocal Imaging and Biomedical Image Analysis)

  • 이임걸
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1995년도 추계학술대회
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    • pp.257-261
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    • 1995
  • Confocal laser scanning microscopy (CLSM)는 기존의 coherent or incoherent microscopic imaging 보다 횡축 방향 (lateral direction)으로 고해상도를 가지며, 층과 층 사이를 구분하는 광축 방향 (axial direction)의 optical sectioning에 의해 샘플의 3D 구조를 고해상도로 영상화함으로써 3D 구조 및 생체 기능 분석을 가능하게 해 준다. 본 논문에서는 CLSM에 의한 3D 영상화 원리와 촛점면 부근에서 얻어지는 광세기 분포, 얻어진 2D slice 영상의 시각화 및 응용에 대해 논의된다.

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Chloroplasts morphology investigation with diverse microscopy approaches and inter-specific variation in Laurencia species (Rhodophyta)

  • Paradas, Wladimir Costa;Andrade, Leonardo Rodrigues;Salgado, Leonardo Tavares;Collado-Vides, Ligia;Pereira, Renato Crespo;Amado-Filho, Gilberto Menezes
    • ALGAE
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    • 제30권4호
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    • pp.291-301
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    • 2015
  • The present study described with different microscopy approaches chloroplasts lobes in Laurencia sensu latu (Rhodophyta) species and found inter-specific differences among them. Chloroplasts were investigated using confocal laser scanning microscopy (LSM), transmission electron microscopy (TEM) and high resolution scanning electron microscopy (HRSEM). Using and TEM and HRSEM images we distinguished chloroplasts with lobes than chloroplasts without lobes in Yuzurua poiteaui var. gemmifera (Harvey) M. J. Wynne and Laurencia dendroidea J. Agardh cortical cells. The LSM images showed chloroplasts lobes (CLs) with different morphologies, varying from thicker and longer undulated projections in Y. poiteaui var. and L. dendroidea to very small and thin tubules as in Laurencia translucida Fujii & Cordeiro-Marino. The diameter and length of CLs from Y. poiteaui var. and L. dendroidea were significantly higher than L. translucida CLs (p < 0.01). Based on LSM observations, we suggest that lobes morphology has a taxonomic validity only to characterize L. translucida species.

공초점 현미경용 장초점 마이크로렌즈 제작 (Fabrication of Micro-Lens Array with Long Focal Length for Confocal Microscopy)

  • 김기홍;임형준;정미라;이재종;최기봉;이형석;도이미
    • 한국생산제조학회지
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    • 제20권4호
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    • pp.472-477
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    • 2011
  • This paper shows the method of fabrication of a micro lens array comprised of a Nipkow disk used in a large-area, high-speed confocal microscopy. A Nipkow disk has two components, a micro lens array disk and a pinhole array disk. The microlens array focuses illumination light onto the pinhole array disk and redirects reflected light from a surface to a sensor. The micro lens which are positioned in order on a disk have a hemispheric shape with a few tens of micron in diameter, and can be fabricated by a variety of methods like mechanical machining, semiconductor process, replication process like imprinting process. This paper shows how to fabricate the micro lens array which has a long focal length by reflow and imprinting process.

A TISSUE RESPONSE TO THE TITANIUM ALLOY (Ti-13Zr-6Nb) IN VIVO

  • Kim Chang-Su;Lee Seok-Hyung;Shin Sang-Wan;Suh Kyu-Won;Ryu Jae-Jun
    • 대한치과보철학회지
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    • 제42권6호
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    • pp.619-627
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    • 2004
  • Statement of problem. Mechanisms of tissue-implant interaction and the effect of the implant surface on the behavior of cells has not yet been clarified. Purpose. This study was performed to investigate the tissue reaction to the titanium alloy submerged into rat peritoneum in vivo. Materials and methods. Titanium alloys (titanium-13Zirconium-6Niobium) were inserted inside the peritoneal cavity of Sprague Dawley rats. After 3 months, the tissue formed around the inserted titanium alloys were examined with a light-microscope. Tissue reaction around the material was analyzed by confocal microscopy to evaluate their biocompatibility in a living body. Results. In in vivo study, foreign body type multinucleated giant cells were found in the fibrous tissue formed as a reaction to the foreign material (4 in 20 cases), but the inflammatory reaction was very weak. After experiment, the contaminants of biomaterials was removed from living tissue. In confocal microscopy, we observed that the staining of vinculin and actin showed mixed appearance. In a few cases, we found that the staining of vinculin and beta-catenin showed the prominent appearance. Conclusion. We found that titanium-13Zirconium-6Niobium alloy was an excellent biomaterial.

Rapid Detection of Methicillin Resistant Staphylococcus aureus Based on Surface Enhanced Raman Scattering

  • Han, Dae Jong;Kim, Hyuncheol
    • 대한임상검사과학회지
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    • 제46권4호
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    • pp.136-139
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    • 2014
  • Methicillin-resistant Staphylococcus aureus (MRSA) is one of the severe nosocomial infectious agents. The traditional diagnostic methods including biochemical test, antibiotic susceptibility test and PCR amplification are time consuming and require much work. The Surface enhanced Raman spectroscopy (SERS) biosensor is a rapid and powerful tool for analyzing the chemical composition within a single living cell. To identify the biochemical and genetic characterization of clinical MRSA, all isolates from patients were performed with VITEK2 gram positive (GP) bacterial identification and Antibiotic Susceptibility Testing (AST). Virulence genes of MRSA also were identified by DNA based PCR using specific primers. All isolates, which were placed on a gold coated nanochip, were analyzed by a confocal Raman microscopy system. All isolates were identified as S. aureus by biochemical tests. MRSA, which exhibited antibiotic resistance, demonstrated to be positive gene expression of both femA and mecA. Furthermore, Raman shift of S. aureus and MRSA (n=20) was perfectly distinguished by a confocal Raman microscopy system. This novel technique explained that a SERS based confocal Raman microscopy system can selectively isolate MRSA from non-MRSA. The study recommends the SERS technique as a rapid and sensitive method to detect antibiotic resistant S. aureus in a single cell level.

공초점 현미경을 이용한 마이크로믹서 내부의 3차원 이미지화 (3-D Imaging in a Chaotic Micromixer Using Confocal Laser Scanning Microscopy (CLSM))

  • 김현동;김경천
    • 한국가시화정보학회:학술대회논문집
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    • 한국가시화정보학회 2006년도 추계학술대회 논문집
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    • pp.96-101
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    • 2006
  • 3-D visualization using confocal laser scanning microscopy (CLSM) in a chaotic micromixer was performed as a reproduction experiment and the feasibility of 3-0 imaging technique in the microscale was confirmed. For diagonal micromixer (DM) and two types of staggered herringbone micromixers (SHM) designed by Whitesides et al., to verify the evolution of mixing, cross sectional images are reconstructed at the end of every cycle. In a DM, clockwise rotational flow motion generated by diagonal ridges placed on the floor of micromixer is observed and this motion makes the fluid commingle. On the contrary, there are two rotational flow structures in the SHM and the centers of rotation exchange their position each other every half cycle because of the V shape of ridges varying their orientation every half cycle. Local rotational flow and local extensional flow generated by the complicate ridge pattern make the flow be chaotic and accelerate the mixing of fluid. The dominant parameter that influences on the mixing characteristic of SHM is not the length of micromixer but the number of ridges under the same flow configurations.

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Signal increasing method in confocal scanning microscopy in fluorescence mode using curved mirror

  • Kang, Dong-kyun;Seo, Jung-woo;Gweon, Dae-gab
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 2001년도 ICCAS
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    • pp.99.3-99
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    • 2001
  • In fluorescence mode confocal scanning microscope, level of detected signal is very low. In object scanning type confocal scanning microscope, the additional optical system with objective lens and plane mirror was proposed to increase signal intensity, but there was none for beam scanning type confocal scanning microscope. We propose reflecting optical systems which improve signal intensity in beam scanning type confocal scanning microscope. We choose one of the proposed optical systems and design the optical system, i.e., select optical components and assign distances between the selected components. To design the optical system, we use finite ray tracing method and make cost function to be minimized.

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