• Journal of Pharmacopuncture
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• v.16 no.1
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• pp.30-36
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• 2013

Objective: Panax ginseng is one of the most medicinally used herbal medicines in the world. Wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the differences between wild ginseng and cultivated ginseng. Method: To identify wild ginseng-specific genes, we used suppressive subtraction hybridization. Results: We report that one of the clones isolated in this screen was the GAPDH (glyceraldehyde 3-phosphate dehydrogenase) gene (designated pGAPDH-w). DNA BLAST sequence analysis revealed that this pGAPDH-w gene contained novel sequences of 94 bp. RT-PCR results showed that the expression of the pGAPDH-w gene was significantly up-regulated in the wild ginseng as compared with the cultivated ginseng. Conclusion: The pGAPDH-w gene may be one of the important markers of wild ginseng.

• Journal of Pharmacopuncture
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• v.15 no.1
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• pp.18-22
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• 2012

Panax ginseng is a well-known herbal medicine in traditional Asian medicine. Although wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Using suppressive subtraction hybridization, we cloned the p-psbB gene as a candidate target gene for a wild ginseng-specific gene. Here, we report that one of the clones isolated in this screen was the chloroplast p-psbB gene, a chlorophyll a-binding inner antenna protein in the photosystem II complex, located in the lipid matrix of the thylakoid membrane. Real-time results showed that the expression of the p-psbB gene was significantly up-regulated in wild ginseng as compared to cultivated ginseng. Thus, the p-psbB gene may be one of the important markers of wild ginseng.

• Journal of Ginseng Research
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• v.37 no.4
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• pp.491-495
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• 2013

Wood-cultivated ginseng, including roots in its dried form, is produced in forest land without using artificial facilities such as light barriers. To identify suitable sites for the propagation of wood-cultivated ginseng, factor combination technique (FCT) and linear combination technique (LCT) were used with geographic information system and the results were superimposed onto an actual wood-cultivated ginseng plantation. The LCT more extensively searched for suitable sites of cultivation than that by the FCT; further, the LCT probed wide areas considering the predominance of precipitous mountains in Korea. In addition, the LCT showed the much higher degree of overlap with the actual cultivation sites; therefore, the LCT more comprehensively reflects the cultivator's intention for site selection. On the other hand, the inclusion of additional factors for the selection of suitable cultivation sites and experts' opinions may enhance the effectiveness and accuracy of the LCT for site application.

• Journal of Pharmacopuncture
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• v.14 no.4
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• pp.33-37
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• 2011

Objectives: To report the effect of cultivated wild ginseng pharmacopuncture on the lung cancer patient. Methods: Treatment of cultivated wild ginseng pharmacopuncture was conducted on the patient during 7 months. Pharmacopuncture therapy was done twice a week with a dosage of 2ml per each treatment. Computed Topography(CT) was performed to evaluate the therapeutic efficacy. Results: After the treatment of 7 months, chest CT revealed the mass size was decreased. Conclusions: This case provides us a possibility that cultivated wild ginseng pharmacopuncture offers potential benefits for lung cancer patient.

• Journal of Acupuncture Research
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• v.22 no.2
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• pp.211-217
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• 2005

Objectives : Terminal stage cancer patient from primary hepatocellular carcinoma metastasized into lungs was administered with cultivated wild ginseng herbal acupuncture for 5 months and observed progression. Methods : Cultivated wild ginseng herbal acupuncture was administered 5 times a week at about 150cc dosage per week. Dynamic CT was taken and interpreted at a university hospital. Results : Above patient was diagnosed with hepatocellular carcinoma and received one procedure of lobectomy and three procedures of TACE, but because of metastasis, chance of improvement was very obscure. Intensive treatment of cultivated wild ginseng herbal acupuncture five times a week for five months in association with moxibustion was done on the patient. Near elimination of the cancer cells metastasized into lungs were confirmed in terms of radiological impression through dynamic CT. Conclusion: From the results obtained in this study, cultivated wild ginseng herbal acupuncture can be an effective measure against terminal stage cancer. But this is a single case study and lack of extensive follow-up must be compensated by further researches.

• Journal of Pharmacopuncture
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• v.14 no.1
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• pp.35-49
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• 2011

Objectives: The aim of this experiment is to know about Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture. Methods: First 20 Middle Aged women are diagnosed by pulse diagnosis, and then Mountain Cultivated Ginseng Pharmacopuncture(1 injection 20cc) were injected. 30 minutes later, pulse diagnosis again performed. As a result, method of one-group pretest-posttes design were used for evaluation. Results: T(Total pulse cycle) time statistically significant increased on both left and right chon, kwan, cheok. T4 time statistically significant increased on both left and right chon, kwan, cheok. T4-T1/T indexs except left cheokmaek, Right cheokmaek observation area decreased significantly in four sites. Wm(indicating high pressure retention time) indexs increased significantly in the five sites were observed except right chon maek. Conclusions: Effect of pulse-wave factors in Middle Aged Women by Mountain Cultivated Ginseng Pharmacopuncture increased T, T4, Wm and decreased T4-T1/T indexs. The results of this experiment, Mountain Cultivated Ginseng Pharmacopuncture induced to increase the Pulse-wave's stability and strength.

• Journal of Korean Society of Forest Science
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• v.97 no.4
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• pp.357-362
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• 2008

We compared the morphological characters and stable isotopes of seeds of wild simulated ginseng with those of cultivated field ginseng. Seeds were collected from ten areas for wild simulated ginseng and two areas for cultivated field ginseng. The length, width, thickness and number of seeds per gram were measured and the ratio of stable isotopes of carbon and nitrogen was analysed as well. There was a overlapping variation of morphological characters and the ratio of stable isotope of the wild simulated ginseng among study areas. In Geumsan area, compared with the seed of cultivated field ginseng, the seed of wild simulated ginseng was significantly small and light, and the ${\delta}^{15}N$(‰) of wild simulated ginseng was lower than that of cultivated field ginseng. However, it is somewhat impossible to compare the regional variation of the unique value of ${\delta}^{15}N$(‰) among study areas of wild simulated ginseng. We can suggest the value of ${\delta}^{15}N$(‰) can be used as detection factor for cultivation regime like chemical fertilization and organic farming.

• Journal of Ginseng Research
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• v.40 no.4
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• pp.344-350
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• 2016

Background: Mountain-cultivated ginseng (MCG) and cultivated ginseng (CG) both belong to Panax ginseng and have similar ingredients. However, their pharmacological activities are different due to their significantly different growth environments. Methods: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS)-based approach was developed to distinguish MCG and CG. Multivariate statistical methods, such as principal component analysis and supervised orthogonal partial-least-squares discrimination analysis were used to select the influential components. Results: Under optimized UPLC-QTOF-MS/MS conditions, 40 ginsenosides in both MCG and CG were unambiguously identified and tentatively assigned. The results showed that the characteristic components of CG and MCG included ginsenoside Ra3/isomer, gypenoside XVII, quinquenoside R1, ginsenoside Ra7, notoginsenoside Fe, ginsenoside Ra2, ginsenoside Rs6/Rs7, malonyl ginsenoside Rc, malonyl ginsenoside Rb1, malonyl ginsenoside Rb2, palmitoleic acid, and ethyl linoleate. The malony ginsenosides are abundant in CG, but higher levels of the minor ginsenosides were detected in MCG. Conclusion: This is the first time that the differences between CG and MCG have been observed systematically at the chemical level. Our results suggested that using the identified characteristic components as chemical markers to identify different ginseng products is effective and viable.

• Journal of agriculture & life science
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• v.45 no.2
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• pp.9-13
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• 2011

This study was carried out to control the disease of wood-cultivated ginseng(panax ginseng) using natural materials. Four fungi spices such as Pythium ultimum, Alternaria alternata, Fusarium oxysporum and Rhizoctonia solani which caused disease of the wood-cultivated ginseng were investigated. The infection of these fungi causes symptom on roots, stems and leaves. The leaves became dry and die. The disease caused by Pythium ultimum can be prevented by using friendly environmental materials like Chamaecyparis obtuse essential oil and Wormstop. Alternaria alternata and Fusarium oxysporum might be prevented by using wormstop extracted from Neem tree(Azadirachta indica). No substance tested effectively prevents the growth of Rhizoctonia solani.

• Journal of Applied Biological Chemistry
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• v.62 no.1
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• pp.87-91
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• 2019

The contents of major ginsenosides (ginsenosides Rb1, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, and ginsenoside Rg1) in ginseng cultivated in different areas in Korea, ginseng that underwent different cultivation processes and ages, and ginseng cultivated in different countries were determined using high-performance liquid chromatography equipped with UV/VIS detector. Ginsenoside Rc was the most abundant ginsenoside in all different ginseng samples. The highest total concentration of major ginsenosides was found in the ginseng cultivated in Jinan (0.931 mg/g) and 4-year grown red ginseng (1.785 mg/g). Major ginsenosides were the most abundant in Korean ginseng (1.264 mg/g), compared to those in Chinese and American ginseng. The results of this study showed the different contents of major ginsenosides in the ginseng samples tested and emphasized which sample could contain high yield of ginsenosides.