• Title/Summary/Keyword: dead-end flitration

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Separation of Lysozyme from Chicken Egg White by Ultrafiltration (1) - Dead-end Filtration of Egg White Solution - (한외여과에 의한 계란 난백으로부터 라이소자임의 분리 (1) - 난백용액의 전량여과 -)

  • Park, Hye-Ri;Youm, Kyung-Ho
    • Membrane Journal
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    • v.26 no.4
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    • pp.266-272
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    • 2016
  • The separation and purification of lysozyme enzyme from chicken egg white (CEW) solution was studied using the dead-end filtration. The optimum operation conditions of the dead-end filtration reveal that the maximum value of permselectivity of lysozyme to the other proteins of ovalbumin and conalbumin in the CEW solution was tested with change of molecular weight cut-off (MWCO) of ultrafitration membrane and pH of the CEW solution. The optimum operation conditions for the efficient permeable separation of lysozyme from the CEW solution are that the membrane MWCO is 30 kD and the pH of CEW solution is 11. At this optimum operation conditions, the maximum value of permselectivity of lysozyme to total proteins in CEW solution is about 83.