• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.17 no.1
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• pp.94-103
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• 2004

Objective: This study was performed to detennine the depigmenting effects of Kamibongpungtongsung-San. Methods: To determine the depigmenting effects of Kamibangpungtongsung-San. we measured the degree of tyrosinase inhibition, melanin production & cell viability in cultured B16 melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. Results: Komibangpungtongsung-San did not show inhibitory effects on melanin production in melanoma cells, UV screen and cytoprotective effects on PC12 cells injured by hydrogen peroxide. However it showed mild inhibitory effects on tyrosinase activity. Conclusion : This study shows that Kamibangpungtongsung-San, a generally used prescription for dermatologic diseases, do not have depigmenting effects via tyrosinase inhibition. Therefore, the depigmenting effect and mechanism of depigmentation by Kamibangpungtongsung-San need to be evaluated and investigated in other directions.

• Korean Journal of Medicinal Crop Science
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• v.16 no.3
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• pp.192-194
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• 2008

In our previous study, ethylacetate fraction of white ginseng (root of Panax ginseng C.A. Meyer) extract inhibited mushroom tyrosinase activity and melanin production in melanocytes. This study examined its effects on the expression of melanin biosynthesis-related enzymes to explore the depigmenting pathway. Moreover, depigmenting effect on animal skin was examined using UV-B induced hyperpigmented skin of brown guinea pigs. The ethylacetate fraction of the white ginseng extract exhibited depigmenting activity in the skin of brown guinea pig without visible edema. In addition, this fraction reduced tyrosinase expression in melanocytes. The results suggested that ethylacetate fraction of white ginseng extract might be used as skin depigmenting material by inhibition of tyrosinase activity and expression.

• Bulletin of the Korean Chemical Society
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• v.30 no.7
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• pp.1619-1621
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• 2009

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1585-1590
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• 2007

Although a number of melanogenesis inhibitors have recently been reported and used as cosmetic additives, none is completely satisfactory, leaving a need for novel skin-depigmenting agents. Thus, to develop a novel skin depigmenting agent from natural sources, the inhibition of melanogenesis by Chinese plants was evaluated. A methanolic extract of Nigella glandulifera Freyn was found to inhibit the melanin synthesis of murine B16F10 melanoma cells by 43.7% and exhibited a low cytotoxicity (8.1%) at a concentration of $100\;{\mu}g/ml$. Thus, to identify the melanogenesis-inhibiting mechanism, the inhibitory activity towards tyrosinase, the key enzyme of melanogenesis, was further evaluated, and the results showed inhibitory effects on the activity of intracellular tyrosinase yet not on mushroom tyrosinase. Finally, to isolate the compounds with a hypopigmenting capability, activity-guided isolation was performed, and Dioctyl phthalate identified as inhibiting melanogenesis.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.174-183
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• 2003

To obtain effective and safe topical depigmenting agents, we synthesized hydroxybenzoates, alkoxybenzoates, and 3,4,5-trimethoxycinnamate containing a thymol moiety and screened then for high-level inhibitory activity against melanin synthesis. Among them, 5-methyl-2-(methylethyl)phenyl (2Ε)-3-(3,4,5-trimethoxyphenyl)prop-2-enoate (Melasolv)$^{TM}$ 4h, showed the most potent depigmenting effect ($IC_{50}$/ = 10$\mu$M) with low cytotoxicity ($IC_{50}$/ = 200$\mu$M). To find the inhibition mechanism of our candidate, various in vitro tests were performed such as DPPH assay, tyrosinase activity in mushroom or in culture cell and expression of tyrosinase, TRP-l and TRP-2. The result of this study suggested that 4h inhibited melanin synthesis by reducing the expression of tyrosinase and TRP-l at the transcriptional level in melan-a melanocytes.s.

• Bulletin of Food Technology
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• v.25 no.2
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• pp.150-153
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• 2012

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.222-222
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• 2005

• Journal of the Society of Cosmetic Scientists of Korea
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• v.35 no.1
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• pp.1-9
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• 2009

South Asian's growing obsession with fair skin has made the cosmetic industry into a multibillion-dollar trade over the last decade alone. With reports of toxicity and potential mutagenicity of conventional skin lightening agents, cosmetic industries are looking for plant-based skin whitening formulations. In this review some potential depigmentation agents from South Asian region are discussed, including their historical background, biochemical characteristics and recent findings on their depigmenting activity.

• Bulletin of the Korean Chemical Society
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• v.33 no.4
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• pp.1333-1336
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• 2012

• Journal of the Society of Cosmetic Scientists of Korea
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• v.34 no.3
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• pp.183-188
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• 2008

Initial screening assay for depigmenting agents includes in vitro mushroom tyrosinase assay and antioxidant assay. Based on this screening result, melanin synthesis in melanocyte, in screened samples, is further measured. Measuring cellular melanin needs time, human resource, and skills. Therefore initial screening method should be reliable. We examined, 34 Chinese herbs, correlated the screening assay methods with cellular melanin. No reliable relationship was observed between factors, indicating the limitation in the use of these assays, probably due to the complexicity of melanogenesis.