• 대한의용생체공학회:의공학회지
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• 제25권6호
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• pp.557-563
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• 2004

당뇨병 환자의 혈당검사시 통증을 최소화하는 새로운 진공 자동 채혈기법을 개발하였다. 특별히 고안, 제작된 채혈기구를 사용하여 전완부위에서 채혈침으로 피부를 관통한 직후 자동적으로 -100mmHg의 진공압력이 가해짐으로써 모세혈액이 흡입되는 자동화된 진공 채혈기법을 구현하였다. 진공 자동 채혈기법의 유용성을 검증하기 위하여 전완부위에서의 채혈용적을 측정하였다. 58명의 정상인을 대상으로 464회의 채혈을 시행한 결과 평균 채혈용적은 약 2.6$\muL$이었고 0.5$\muL$이상의 채혈빈도가 약 86%이었다. 최근 개발되고 있는 혈당계들은 0.3~0.5$\muL$의 모세혈액으로 혈당검사가 가능하므로 검사성공률 역시 약 86%로 추정되었다. 채혈통증 감소효과를 평가하기 위하여 손가락 및 전완부위 채혈 후 시각상사 통증척도를 사용하여 통증지수를 측정하였다. 전완부위 채혈시 통증은 손가락 채혈의 약 23%에 불과하였으며 거의 무통증 채혈이 가능하였다. 따라서 본 연구의 전완부위 진공 자동채혈기법은 전통적인 손가락 채혈에 비해 채혈통증이 25% 이하로 현저하게 감소하지만 검사실패율이 14%로써 경우에 따라 재채혈이 필요할 수 있다. 그러나 매일 수 차례 채혈해야 하는 만성 당뇨환자의 경우 검사기피를 방지할 수 있는 유용한 방법으로 생각된다.

• 대한치과보철학회지
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• 제30권4호
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• pp.541-564
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• 1992

The purpose of this experiment was to study the effect of implantation of the dense hydroxyapatite (HA) granules$(Apaceram^{(R)})$ in the fresh extraction sockets on the residual ridge resorption in normal and induced diabetic rabbits. Sixty-four white rabbits, weighing 3.0kg, were utilized in this study and they were divided into four group : group 1-sixteen normal rabbits without filling HA granules, group 2-sixteen normal rabbits with filling HA granules, group 3-sixteen diabetic rabbits without filling HA granules, group 4-sixteen diabetic rabbits with filling HA granules. HA granules were filled immediately after extraction in the extraction sockets of the mandibular left 1st, 2nd, and 3rd molar in group 2 and 4, and no HA granules were filled in group 1 and 3. The calculation of residual ridge resoption was done at 4th week, 8th week, 12th week, and 16th week after surgery, and histologic examination was performed to study healing process at 2nd week, 4th week, 8th week, and 16th week after surgery. The results were as follows : 1. The amount of residual ridge resorption was largest in group 3, and smallest in group 2 in every interval. There was no statistically significant difference between group 1 and 4. 2. In all groups, the amount of residual ridge resorption was high until 4th week after surgery, but the rate of residual ridge resolution slowly decreased after 4th week 3. There was more active healing process in group 1 and 2 than in group 2 and 4. 4. According to filling of HA granules, there was no difference in the amount of new bone formation, but direct contact could be observed between newly formed bone and HA granules in group 2 and 4. 5. Group 1 and 3 showed marked bone resorption and adipose marrow from 8th-16th week after surgery, but group 2 and 4 did not show maeked bone resolution, and showed fibrous tissue and a little adipose tissue among trabeculae from 8th week.

• 한국식품영양과학회지
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• 제31권3호
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• pp.500-505
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• 2002

뇌조직에서 고혈당으로 인한 산화적 스트레스에 대한 민들레의 유해 활성산소 생성과 제거효소계에 미치는 영향을 알아보고자 streptozotocin으로 당뇨를 유발한 Wistar계 횐쥐에게 민들레의 잎과 뿌리의 분말과 열수추출물을 각각 4주간 급여하였다. 실험결과 유해 활성산소 생성효소계인 시토크롬 P450 함량, aminopyrine N-demethylase, aniline hydroxylase 및 xanthine oxidase 활성은 당뇨를 유발한 대조군에 비하여 서양민들레 잎과 뿌리 급여군 모두 감소되었다. Superoxide dismutase, catalase와 glutathione peroxidase 활성 역시 서양민들레의 분말과 열수추출물 급여시 대조군에 비하여 유의적인 감소를 나타내었으며 민들레의 부위에 따른 차이는 관찰되지 않았다. 반면 뇌조직 중의 glutathione S-transferase 활성과 GSH 함량은 대조군에 비 하여 서양민들레 급여시 유의적으로 증가되었으며, 과산화지질 함량은 당뇨 대조군에 비하여 서양민들레 급여군 모두 유의적인 감소를 보였다. 이상의 결과에서 서양민들레의 잎과 뿌리의 급여는 당뇨로 인한 횐쥐의 뇌조직 중 유리기 생성과 지질과산화로 인한 합병증 예방에 효과적일 것으로 사료된다.

• 한국한의학연구원논문집
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• 제16권1호
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• pp.119-134
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• 2010

Objectives & Method：Based on the statistic from January 2008 to December 2008 include 66-year-old 375 people (159 men, 216 women) who got examined in National Health Insurance Corporation, this study set a goal to establish a new health index by analyzing the relation between the cardio-cerebra vascular disease and risk factor such as obesity, smoking, exercise, alcohol consumption, blood pressure, blood sugar and cholesterol. Results：1) Examine results, health status according to gender；People who are diagnosed as abnormal health status have higher risk for both men and women. As for the detailed diseases, high blood pressure was highest followed by hyperlipidemia and obesity. In case of the women, 96.3% in bone mineral density was highest. 2) Correlation of disease and health risk factors based on gender；Correlation of disease according to gender in the obesity(p<0.001), lipid abnormalities(p<0.001), kidney diseases(p<0.001), chest diseases(p<0.05), osteoporosis(p<0.001) showed a statistically significant difference. The analysis showed that the men have more obesity and lipid abnormalities as well as thoracic disease than the women, but the women have more kidney disease than the men. As for the osteoporosis examine which is conducted only for the women, most of the women were abnormal even though it cannot compare the result according to gender. Obesity(p<0.001), alcohol(p<0.001), smoking(p<0.001) and LDLcholesterol(p<0.001) showed statistical correlation between gender and health risk factor. And only high blood pressure(p<0.001) showed a statistical correlation between gender and risk of cardio-cerebra vascular disease. 3) Risk of cardio-cerebra vascular disease related to health risk factor characteristic；Obesity assessment(p<0.001), alcohol(p<0.05), smoking(p<0.05), blood pressure(p<0.001), glucose(p<0.001) showed correlation between health risk factor and stroke. Drinking(p<0.001), smoking(p<0.05), exercise(p<0.001), blood pressure(p<0.001), LDL cholesterol(p<0.001) showed health risk factor and myocardial infarction. Obesity assessment(p<0.001), smoking(p<0.05), blood pressure(p<0.001), glucose(p<0.001) and showed correlation between health risk factor and diabetes. Obesity assessment(p<0.001), blood pressure(p<0.001) showed correlation between health risk factor and high blood pressure. Conclusion：Therefore, we have to take one step farther after the health examination. In order to prevent and reduce the risk of disease, people have to understand their health status exactly first, and correct their habit with recognizing the amount of risk related to cardio-cerebra vascular disease.

• 센서학회지
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• 제14권2호
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• pp.69-77
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• 2005

The electronic nose (e-nose) has been used in food industry and quality controls in plastic packaging. Recently it finds its applications in medical diagnosis, specifically on detection of diabetes, pulmonary or gastrointestinal problem, or infections by examining odors in the breath or tissues with its odor characterizing ability. Moreover, the use of portable e-nose enables the on-site measurements and analysis of vapors without extra gas-sampling units. This is expected to widen the application of the e-nose in various fields including point-of-care-test or e-health. In this study, a PDA-based portable e-nose was developed using micro-machined gas sensor array and miniaturized electronic interfaces. The rich capacities of the PDA in its computing power and various interfaces are expected to provide the rapid and application specific development of the diagnostic devices, and easy connection to other facilities through information technology (IT) infra. For performance verification of the developed portable e-nose system, Six different vapors were measured using the system. Seven different carbon-black polymer composites were used for the sensor array. The results showed the reproducibility of the measured data and the distinguishable patterns between the vapor species. Additionally, the application of two typical pattern recognition algorithms verified the possibility of the automatic vapor recognition from the portable measurements. These validated the portable e-nose based on PDA developed in this study.

• 대한약침학회지
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• 제7권1호
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• pp.5-14
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• 2004

Acupuncture has fairly good weight-reducing effect in treating simple obesity due to the neuroendocrine regulation. In this study, the antiobesity effects of herbal acupuncture(HA) with Sang-hwang(phellinus linteus) at Fuai(SP16) were investigated in the rat fed on high-fat(HF) diet. Sang-hwang mushroom has been proven to have anti-carcinogenic effects and Sang-hwang extracts are highly effective in treatment and preventive treatment of AIDS, diabetes and high blood-pressure. To determine whether the Sang-hwang herbal acupuncture may have the anti-obesity effect, male Sprague-Dawley(4-wk-old) rats were fed a HF diet for 5 wk, which produced significant weight gain compared to rats were fed a normal diet, and then herbal acupuncture were treated for 3 wk in HF diet group. The body weight, food consumption, food effeciency ratio(FER), body fat mass, plasma nitric oxide(NO) were investigated in rats fed on normal diet, HF diet, and HF diet with HA(HF-diet-HA) groups. NO has been proposed to be involved in the regulation of food intake. In addition, the expression of appetite peptides such as orexigenic peptide neuropeptide Y(NPY) and the anorectic peptide cholecystokinin(CCK) were observed in the hypothalamus. HF-HA group reduced body weight gain, FER, body fat contents and NO concentration compared to HF diet group. The expression of NPY was reduced in arcuate nucleus(ARC), and CCK was increased in the paraventricular nucleus(PVN) after treatment of HA. In conclusion, Sang-hwang HA reduced adipocity, plasma NO and hypothalamic NPY, but increased CCK expression in the HF dietinduced obesity rat, therefore HA may have anti-obesity action through regulating body weight and appetite peptide of the central nervous system.

• 한국발생생물학회지:발생과생식
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• 제24권3호
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• pp.231-239
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• 2020

Many benefits of silk protein fibroin (SPF) have been suggested in biomedical applications; and notably, significant SPF effects have been observed for metabolic syndromes that are directly linked to insulin resistance, such as type 2 diabetes mellitus (T2DM). Based on our previous findings, we believe that SPF from spiders exhibits outstanding glucose-lowering effects in diabetic BKS.Cg-m+/+Lepr^db mice. In order to evaluate the dietary effects of SPF in diabetic animals, we generated several lines of transgenic rice (TR) that expresses SPF, and the feeding of TR-SPF to diabetic animals decreased blood glucose levels, but did not change insulin levels. Western blot analyses of hepatic proteins showed that AMP-activated protein kinase (AMPK) expression and phosphorylation both decreased in TR-SPF-fed groups, compared with controls. This finding suggests that the glucose-lowering effects in this diabetic animal model might be AMPK-independent. In contrast, six-transmembrane protein of prostate 2 (STAMP2) was upregulated after TR-SPF exposure. Together with STAMP2, the Akt protein phosphorylation increased after TR-SPF exposure, which indicates that STAMP2 leads to Akt phosphorylation and thus increases insulin sensitivity in hepatocytes. Importantly, the hepatic steatosis that was seen in the liver of diabetic mice was remarkably alleviated in TR-SPF-fed mice. Hepatocytes that were immunopositive for STAMP2 were overwhelmingly observed in hepatic tissues from TR-SPF-fed mice compared to the control. Taken together, these results suggest that feeding diabetic mice with TR-SPF upregulates STAMP2 expression and increases Akt phosphorylation in hepatic tissues and thus potentially alleviates insulin resistance and hepatic steatosis.

• 한국환경농학회지
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• 제34권1호
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• pp.57-63
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• 2015

최근 들어 여주의 혈당개선의 약리학적 효능이 보고됨에 따라 기능성 채소로 각광받고 있다. 주로 여주 과실만이 식용으로 쓰이지만 여주씨 또한 다양한 가공과정을 거칠 경우(건여주 혹은 건조분말, 환, 엑기스 등) 필연적으로 함께 섭취되기도 한다. 본 연구에서는 과실이외의 특히, 연구결과가 미비한 여주씨에 대해 생리활성물질의 구명 및 혈당개선의 효과에 대한 연구를 수행하였다. 다양한 추출물 및 단일 활성성분의 혈당개선 in vitro검증법인 ${\alpha}$-glucosidase에 대한 저해효과를 측정하였고, 활성을 나타내는 추출물 또는 활성성분과의 상관관계를 구명하였다. 구체적으로는 95% 에탄올 추출물 및 비극성 용매 등에 다량 추출되는 생리활성물질인 ${\alpha}$-eleostearic acid는 우수한 저해효과를 나타내었다($IC_{50}=43.4{\mu}g/mL$). 또한 효소저해제의 함량을 HPLC를 통한 정량분석을 수행함으로써 여주씨에 함유된 기능성성분의 혈당개선 식 의약 소재로의 응용가능성을 연구하였다.

• 농약과학회지
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• 제11권3호
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• pp.171-178
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• 2007

소나무 수피와 잣나무 수피를 먹이로 사육한 북방수염하늘소(M. saltuarius)의 종령유충의 소화액을 추출하여 4가지 기질(${\alpha}$-naphthyl acetate, ${\beta}$-naphthyl acetate, ${\alpha}$-naphthyl butyrate, ${\beta}$-naphthyl stearate)에 대한 에스테라제(esterase) 활성도 변화를 조사하였다. ${\alpha}$-naphthyl acetate를 기질로 하여 조사한 결과 Est7이 잣나무수피를 먹인 유충에서 강하게 활성화되었다. 또한, ${\beta}$-naphthyl acetate와 ${\alpha}$-naphthyl butyrate 기질을 사용한 경우에도 Est6과 Est7이 잣나무수피를 먹인 유충에서 강한 활성이 관찰 되었다. 그러나 ${\beta}$-naphthyl stearate에서 활성화 되는 에스테라제는 관찰할 수 없었다. 추출한 소화액에 카바메이트계와 유기인계 약제를 처리한 경우 Est7을 포함하여 Est3과 Est6이 강하게 저해되는 것으로 나타났다. 또한, eserine을 처리한 경우 Est4, Est6, Est7에서 강한 활성저해가 관찰되었다. Methoprene, bornyl acetate, linal, cineol, citral을 소화액에 첨가한 경우 에스테라제의 활성저해는 관찰되지 않았으나 이상의 결과는 in vitro에서 실시된 실험으로 추후 in vivo에서 재검증이 필요 할 것이다.

• 한국산학기술학회논문지
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• 제12권2호
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• pp.765-774
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• 2011

본 연구는 효율적인 당뇨관리사업을 할 수 있는 기초자료를 제공하기 위해 수행되었다. 연구를 위해 2007년, 2008년도 국민건강영양조사를 통해 검진에 참여한 당뇨인지환자 666명의 자료를 수집하여 분석하였다. 당뇨인지 환자의 관리행태에 대한 군집분류는 K-means 기법을 이용하였고 관리행태에 대한 요인분석은 의사결정나무와 다중로지스틱 회귀분석을 이용하였다. 당뇨인지환자의 군집은 크게 3개로 분류되었다. 건강행태사업 대상군은 당뇨 치료와 합병증 검사는 잘 받고 있으나 음주, 흡연, 운동실천 등 건강행태 개선이 보다 적극적으로 이루어져야 하는 집단이다, 중점관리사업 대상군은 치료를 제대로 받지 않고, 합병증검사도 하지 않으며 혈당관리를 위한 건강행태 개선도 적극적으로 하지 않는 집단이다, 합병증검사사업 대상군은 치료를 잘 받고 있으며 건강행태도 개선하고 있지만 급만성 합병증을 조기 발견하기 위한 합병증검사를 소흘히 하는 집단이다. 군집을 분류하는데 가장 중요한 요인은 고지혈증 유무로 나타났으며 그 외 성, 소득, 연령, 직업, 주관적 건강상태도 주요한 변수였다. 당뇨 조절율을 향상시키기 위해서는 각 군집의 특성에 따라 보다 특화된 당뇨관리 프로그램이 적용되어야 할 것이다.