• Applied Microscopy
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• v.42 no.3
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• pp.147-150
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• 2012

A recently introduced helium ion microscopy (HIM) was employed to observe uncoated pine leaf specimens. Adult leaves were collected from the seedlings of Pinus densiflora and P. rigida, air-dried at room temperature, and observed by HIM without metal coating. Ovoid or round stomata and distinct Florin rings could be discerned. The epicuticular waxes were present in the epistomatal chambers and Florin rings of stomata on the leaf surface. The epicuticular waxes were mostly straight, cylindrical, and ca. 1 ${\mu}m$ in length. The epistomatal chambers of P. rigida were filled with the epicuticular waxes, whereas those of P. densiflora were not filled with the epicuticular waxes. Based on their micromorphology, the epicuticular wax structures of the pine species were identified as tubules. These results suggest that the HIM could be used for the investigation of the plant stomata and epicuticular waxes of uncoated plant leaves. Due to the smaller ion probe and interaction volume, the HIM has advantages over conventional field emission scanning electron microscopy in terms of image resolution and charge neutralization.

• Korean Journal of Agricultural Science
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• v.13 no.1
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• pp.62-67
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• 1986

This electron microscopical study was carried out to observe the plasmodesmata of endosperm cells and epicuticular waxes of pericarp ribs on lettuce seed. The morphological modification of pericarp and pericarp ribs was detected by SEM observation. The seed which formed under low temperature, has well developed, suberized cell walls and epicuticular waxes while the seed fromed under high temperature condition has very poorly developed epicuticular waxes. Epicuticular waxes was considered to take over the function of seed protection and keeping the good quality during storage period. The plasmodesmata of endosperm cells in lettuce seed was observed at 4hrs after imbibition for the first time. This phenomenon indicates that the lettuce seed starts its physiological activities in the very early imbibition stages.

• Applied Microscopy
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• v.42 no.3
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• pp.124-128
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• 2012

Leaf surface structures were investigated in the Chinese juniper Juniperus chinensis by scanning electron microscopy. Adult scale leaves were collected from the tree, air-dried at room temperature, and sputter-coated with gold without further specimen preparation. Approximately fi ve stomata were locally distributed and arranged in clusters on the leaf surface. Stomata were ovoid and ca. 40 ${\mu}m$ long. The epicuticular wax structures of J. chinensis leaves were tubules and platelets. Numerous tubules were evident on the leaf regions where stomata were found. The tubules were cylindrical, straight, and ca. 1 ${\mu}m$ in length. They almost clothed the stomatal guard cells, and occluded the slit-shaped stomatal apertures. Moreover, the wax ridges were flat crystalloids that were connected to the surface by their narrow side. They did not have distinct edges, and their width/height ratio varied. In particular, the wax ridges could be discerned on the leaf regions where stomata were not present nearby. Since the wax ridges did not have distinct edges on their margin, they were identified as platelets. Instances were noted where platelets were oriented either parallel to each other or perpendicular to the cuticle surface. These results can be used in biomimetics to design the hierarchical structures for mimicking the plant innate properties such as hydrophobicity and self-cleaning effects of the leaf surface.

• Horticultural Science & Technology
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• v.28 no.5
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• pp.762-767
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• 2010

This study was conducted to investigate the influence of formulation of fungicides on epicuticular waxes in 'Campbell Early' grapes. Non-bagged and bagged berries were treated with wettable powder, suspension concentrate and emulsifiable concentrate 15 days after full bloom (DAFB) and 48 DAFB (veraison), respectively. Fruit skin was observed by naked eye and scanning electron microscope (SEM) at harvest. Remarkable white blots were observed on the berries treated 48 DAFB in the order of wettable powder, suspension concentrate, and emulsifiable concentrate. The observation by SEM showed all formulations of fungicides damaged the ultra-structure of epicuticular waxes; also, an unknown substance was observed on treatment with wettable powder and suspension concentrate. Moreover epicuticular wax appeared to be melting on the grapes treated with emulsifiable concentrate. Also, non-bagged grapes showed serious contamination of epicuticular wax by some unknown substance, but bagged grapes had only fungicides' traces.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.185-197
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• 1992

Differences in the amount and chemical characteristics of the epicuticular waxes on rice leaves were studied for the active tillering and heading stages of rice varieties differing widely in gross leaf-surface property and genetics. The amount of waxes on surfaces of rice leaf-blades was determined by extraction with chloroform and chemical composition of the waxes was characterized by thin layer chromatography, gas liquid chromatography and infrared spectrophotometry. The amount of waxes varied by variety and significantly with growth stage. The amount at the heading stage was 1.7 to 3.6 mg/g fresh weight of leaves, which was two to three times as much as that at the tillering stage of 0.8 to 1.8 mg/g fresh weight. The waxes consisted of seven chemical classes, namely diols, fatty acids, fatty alcohols, fatty aldehydes, fatty esters, saturated and unsaturated hydrocarbons. Diols and unsaturated hydrocarbons were identified as new chemical classes of the rice epicuticular waxes. The polar constituents such as dials, fatty acids and fatty alcohols and the non-polars such as fatty aldehydes, fatty esters, and saturated and unsaturated hydrocarbons were identified at the heading stage, but at the tillering stage only the non-polar compounds were identified. In the carbon numbers (C) of the chemical classes, diols were composed entirely of C30 and acids were mainly of C30 and C31. In alcohols, primary alcohols were composed of C13 and C32, and the secondary alcohols were of C14, C16 and / or C30 regardless of the rice varieties. The acid portion of fatty esters, mainly composed of C22 and C23, showed low cabon numbers compared with the aldehydes. The alcohol portion of them showed a wide distribution in carbon numbers from C13 to C26 depending on the rice varieties. Hydrocarbons had odd carbon numbers, consisting mainly of C29 and C31.

• Journal of Ginseng Research
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• v.39 no.2
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• pp.135-140
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• 2015

Background: A leaf cuticle has different structures and functions as a barrier to water loss and as protection from various environmental stressors. Methods: Leaves of Panax ginseng were examined by scanning electron microscopy and transmission electron microscopy to investigate the characteristics and development of the epicuticular structure. Results: Along the epidermal wall surface, the uniformly protuberant fine structure was on the adaxial surface of the cuticle. This epicuticular structure was highly wrinkled and radially extended to the marginal region of epidermal cells. The cuticle at the protuberant positions maintained the same thickness. The density of the wall matrix under the structures was also similar to that of the other wall region. By contrast, none of this structure was distributed on the abaxial surface, except in the region of the stoma. During the early developmental phase of the epicuticular structure, small vesicles appeared on wallecuticle interface in the peripheral wall of epidermal cells. Some electron-opaque vesicles adjacent to the cuticle were fused and formed the cuticle layer, whereas electron-translucent vesicles contacted each other and progressively increased in size within the epidermal wall. Conclusion: The outwardly projected cuticle and epidermal cell wall (i.e., an epicuticular wrinkle) acts as a major barrier to block out sunlight in ginseng leaves. The small vesicles in the peripheral region of epidermal cells may suppress the cuticle and parts of epidermal wall, push it upward, and consequently contribute to the formation of the epicuticular structure.

• Korean Journal of Plant Tissue Culture
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• v.24 no.6
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• pp.323-327
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• 1997

Addition of growth inhibitors such as ancymidol, ABA, chloromequat, and pachlobutrazol into MS medium had no effect to preventing vitrification in cultures of Rehmania glutinosa. Anatomical investigation revealed that vitrified thick leaf tissue in vitro had larger intercellular space with poor development of sponge and pallisade tissue compared to those of in vitro grown glaucous and field grown plants. In vitro grown glaucous leaf had smaller and round type stomata showing distinguishable guard and subsidiary cell than those of reestablished plantlets into soil whereas abnormal stomata and poor development of epicuticular wax on the surface of leaf was observed in verified plantlet.

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.51-57
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• 2011

The microstructure of the leaf epidermis and trichomes of Fallopia sect. Reynoutria are examined using scanning electron microscopy. Fallopia sachalinensis was distinguished from other taxa in this section by its prominent epicuticular wax layer consisting of protruding wax rodlets. In addition, epicuticular rodlets of F. sachalinensis individuals from Ullung Island and Dok Island appear to be thinner than those from other regions, including Japan and Sakhalin. The stomatal size appears to be related to the ploidy level in the sect. Reynoutria, as the hexaploids, octoploids and dodecaploids tend to have larger stomata as compared to tetraploids. Three basic types of trichomes were found in the section; (1) conical unicellular trichomes, (2) uniseriate filiform trichome consisting of 1-8 cells, and (3) peltate glandular trichomes. The trichome types and their distribution appear to be useful in distinguishing the taxa in the section.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.207-211
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• 1997

Laboratory experiments were conducted to the potential ability of bioremediation with bentazon such as determining the absorption, translocation, and metabolism of $^{14}C-Bentazon$ in minari after foliar applications. The absorption and translocation of $^{14}C-bentazon$ were compared when applied to foliar of minari. In foliar applications, 21% was observed in treated leaves, 66% remained in water extracts of leaf surfaces, and 13% was found in the epicuticular wax layer after 2d. Translocation of the herbicide from treated leaves to roots was very low(79 to 9%). Analysis of methanol-soluble extracts of $^{14}C$ indicated that more than 60% of the foliarapplied herbicide was metabolized in all plant sections after 2d. However, 77% or more of the bentazon was degraded in roots and shoots 2d after root absorption. The major metabolite in these experiments was an unknown compound that was less polar than bentazon and 6- and 8-hydroxy bentazon.

• Journal of Plant Biology
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• v.38 no.1
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• pp.79-86
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• 1995

Visible injury symptoms such as necrosis, chlorosis and premature senscence in the leaves of Ginkgo bloba and Pinus thunbergii treated with acid rain of pH 3.2 or below were observed. The epicuticular wax erosions were observed by SEM after exposure to acid rain of pH 2.4 and 3.2 in G. biloba and pH 4.0 below in P. thunbergii. The adaxial epidennal cells and sponge parenchyma cells were compressed, and those were distorted in the leaves of G. biloba treated with simulated acid rain of pH 3.2 or below. However, vascular tissue was intact. With increase of acidity, mesophyll cells were smaller than those of control while intercellular space in mesophyU was increased. In P. thunbergii, sponge parenchyma cells and vascular tissue except epidennis were distorted after exposure to acid rain of pH 2.4. The size change of stomata in foliar injury was not observed, but the stomatal index and size of stomatal aperture in leaves treated with acid rain increased. The stomata of injured leaf were opened in both species examined.amined.