• Title/Summary/Keyword: factor sequences

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Evaluation of randomness of binary random sequence

  • Harada, Hiroshi;Kashiwagi, Hiroshi;Takada, Tadashi
    • 제어로봇시스템학회:학술대회논문집
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    • 1989.10a
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    • pp.979-983
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    • 1989
  • This paper proposes a new concept, called merit factor Fr, for evaluating the randomness of binary random sequences. The merit factor Fr is obtained from the expected values of the autocorrelation function of the binary random sequence. Using this merit factor Fr, randomness of the binary random sequences generated by the random sampling method is evaluated.

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DNA Light-strand Preferential Recognition of Human Mitochondria Transcription Termination Factor mTERF

  • Nam, Sang-Chul;Kang, Chang-Won
    • BMB Reports
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    • v.38 no.6
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    • pp.690-694
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    • 2005
  • Transcription termination of the human mitochondrial genome requires specific binding to termination factor mTERF. In this study, mTERF was produced in E. coli and purified by two-step chromatography. mTERF-binding DNA sequences were isolated from a pool of randomized sequences by the repeated selection of bound sequences by gel-mobility shift assay and polymerase chain reaction. Sequencing and comparison of the 23 isolated clones revealed a 16-bp consensus sequence of 5'-GTG$\b{TGGC}$AGANCCNGG-3' in the light-strand (underlined residues were absolutely conserved), which nicely matched the genomic 13-bp terminator sequence 5'-$\b{TGGC}$AGAGCCCGG-3'. Moreover, mTERF binding assays of heteroduplex and single-stranded DNAs showed mTERF recognized the light strand in preference to the heavy strand. The preferential binding of mTERF with the light-strand may explain its distinct orientation-dependent termination activity.

On the Phase Sequence Set of SLM Scheme for Crest Factor Reduction in OFDM System (OFDM 시스템의 Crest 값을 감소하기 위한 SLM 기법의 위상 시퀀스에 관한 연구)

  • Lim Dae-Woon;Heo Seok-Joong;No Jong-Seon;Chung Ha-Bong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.9C
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    • pp.889-896
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    • 2006
  • The crest factor distribution of orthogonal frequency division multiplexing (OFDM) symbol sequences is evaluated and it is shown that OFDM symbol sequences with a short period are expected to have a high crest factor. The crest factor relationship between two input symbol sequences, Hamming distance D apart is also derived. Using these two results, we propose two criteria for a phase sequence set of the selected mapping (SLM) scheme and suggest the rows of the cyclic Hadamard matrix constructed from an m-sequence as the near optimal phase sequence set of the SLM scheme.

On desirable conditions for a random number used in the random sampling method

  • Harada, Hiroshi;Kashiwagi, Hiroshi;Takada, Tadashi
    • 제어로봇시스템학회:학술대회논문집
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    • 1990.10b
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    • pp.1295-1299
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    • 1990
  • A new method called random sampling method has been proposed for generation of binary random sequences. In this paper, a new concept, called merit factor Fn, is proposed for evaluating the randomness of the binary random sequences generated by the random sampling method. Using this merit factor Fn, some desirable conditions are investigated for uniform random numbers used in the random sampling method.

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Regulatory Sequences in the 5' Flanking Region of Goat β-Casein Gene

  • Huang, Mu-Chiou;Chao, Jiunn-Shiuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1628-1633
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    • 2001
  • A goat ${\beta}$-casein gene was cloned and sequenced. Our previous study had determined the nucleotide sequences of the 5' flanking region and the structural gene including all 9 exons. In the present study, investigations were done on the regulatory sequences in the 5' flanking region of the goat ${\beta}$-casein gene by aligning and comparing it with the same gene from other mammals. The results showed that -200/-1 bp of the 5' flanking sequences contained six conserved clusters, in which the sites of gene expression regulated by the transcription factor and hormone might exist. It showed that fourteen glucocorticoid receptor elements, two cAMP responsive elements, two SV40 virus enhancer core sequences, two OCT-1 binding elements and one CTF/NF-1 binding element were dispersed in the 5' flanking region of goat ${\beta}$-casein gene. Our findings are perhaps valuable for the elucidation of the molecular mechanisms that control the expression of the goat ${\beta}$-casein gene.

Fast Matching Method for DNA Sequences (DNA 서열을 위한 빠른 매칭 기법)

  • Kim, Jin-Wook;Kim, Eun-Sang;Ahn, Yoong-Ki;Park, Kun-Soo
    • Journal of KIISE:Computer Systems and Theory
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    • v.36 no.4
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    • pp.231-238
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    • 2009
  • DNA sequences are the fundamental information for each species and a comparison between DNA sequences of different species is an important task. Since DNA sequences are very long and there exist many species, not only fast matching but also efficient storage is an important factor for DNA sequences. Thus, a fast string matching method suitable for encoded DNA sequences is needed. In this paper, we present a fast string matching method for encoded DNA sequences which does not decode DNA sequences while matching. We use four-characters-to-one-byte encoding and combine a suffix approach and a multi-pattern matching approach. Experimental results show that our method is about 5 times faster than AGREP and the fastest among known algorithms.

Comparative Study of Minimum Ripple Switching Loss PWM Hybrid Sequences for Two-level VSI Drives

  • Vivek, G.;Biswas, Jayanta;Nair, Meenu D.;Barai, Mukti
    • Journal of Power Electronics
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    • v.18 no.6
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    • pp.1729-1750
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    • 2018
  • Voltage source inverters (VSIs) are widely used to drive induction motors in industry applications. The quality of output waveforms depends on the switching sequences used in pulse width modulation (PWM). In this work, all existing optimal space vector pulse width modulation (SVPWM) switching strategies are studied. The performance of existing SVPWM switching strategies is optimized to realize a tradeoff between quality of output waveforms and switching losses. This study generalizes the existing optimal switching sequences for total harmonic distortions (THDs) and switching losses for different modulation indexes and reference angles with a parameter called quality factor. This factor provides a common platform in which the THDs and switching losses of different SVPWM techniques can be compared. The optimal spatial distribution of each sequence is derived on the basis of the quality factor to minimize harmonic current distortions and switching losses in a sector; the result is the minimum ripple loss SVPWM (MRSLPWM). By employing the sequences from optimized switching maps, the proposed method can simultaneously reduce THDs and switching losses. Two hybrid SVPWM techniques are proposed to reduce line current distortions and switching losses in motor drives. The proposed hybrid SVPWM strategies are MRSLPWM 30 and MRSLPWM 90. With a low-cost PIC microcontroller (PIC18F452), the proposed hybrid SVPWM techniques and the quality of output waveforms are experimentally validated on a 2 kVA VSI based on a three-phase two-level insulated gate bipolar transistor.

Cloning of hadA-like Sigma Factor Gene from Streptomyces coelicolor A3(2) (Streptomyces coelicolor A3(2)에서 hrdA유사 Sigma 인자 유전자의 클로닝)

  • Hahn, Ji-Sook;Cho, Eun-Jung;Roe, Jung-Hye
    • Korean Journal of Microbiology
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    • v.32 no.4
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    • pp.264-270
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    • 1994
  • A gene coding for a novel putative $\sigma$ factor of RNA polymerase has been identified from Streptomyces coelicolor A3(2) using Escherichia coli rpoS gene fragment as a probe. The 486 bp rpoS gene fragment was amplified from E. coli genomic DNA by PCR with two synthetic oligonucleotides, the sequences of which were deduced from the amino acid sequences in the regions 2.3 and 4.2 conserved among various bacterial factors. When E. coli genomic DNA fragments were hybridized with cloned rpoS probe, only one band corresponding to rpoS gene (3.2 kb PvuII fragment or 2.3 kb KpnI fragment) was detected. In S. coelicolor, however, two bands were detected both in PvuII digested DNA and SalI digested DNA. 3.5 kb PvuII fragment which binds the rpoS gene probe was cloned (pMS1) from the sublibrary, and the nucleotide sequences of 1.0 kb BamH'/HincII subclone (pBH2) was partially determined. The nucleotide sequences revealed extensive similarity to other $\sigma$ factor genes of S. coelicolor (hrdA, hrdB, hrdC, hrdD), S. aureofaciens (hrdA, hrdB, hrdC, hrdD), Synechococcus species, Pseudomonas aeruginosa, Stigmatella aurantiaca, and Anabaena species. The nucleotide sequences in regions 1.2 and 4 were compared with the corresponding regions of 5 known ${\sigma}$ factor genes of S. coelicolor by multiple alignment. It turned out that the cloned gene is most closely related to hrdA showing 88% amino acid similarity in region 1.2 and 75% in region 4.

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MOLECULAR CLONING OF CHICKEN INTERFERON-GAMMA (닭 인터페론 유전자의 클로닝에 관한 연구)

  • ;Hyun Lillehoj
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 1999.11a
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    • pp.34-50
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    • 1999
  • A cDNA encoding chicken interferon-gamma (chIFN-${\gamma}$) was amplified from P34, a CD4$^{+}$ T-cell hybridoma by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into pUC18. THe sequences of cloned PCR products were determined to confirm the correct cloning. Using this cDNA as probe, chicken genomic library from White Leghorn spleen was screened. Phage clones harboring chicken interferon-gamma (chIFN-${\gamma}$) were isolated and their genomic structure elucidated. The chIFN-${\gamma}$ contains 4 exons and 3 introns spanning over 14 kb, and follows the GT/AG rule for correct splicing at the exon/intron boundaries. The four exons encode 41, 26, 57 and 40 amino acids, respectively, suggesting that the overall structure of IFN-${\gamma}$ is evolutionairly conserved in mammalian and avian species. The 5’-untranslated region and signal sequences are located in exon 1. Several AT-rich sequences located in the fourth exon may indicate a role in mRNA turnover. The 5’-flanking region contains sequences homologous to the potential binding sites for the mammalian transcription factors, activator protein-1(AP-1) activator protein-2(AP-2) cAMP-response element binding protein(CREB), activating transcription factor(ATF), GATA-binding fator(GATA), upstream stimulating factor(USF), This suggests that the mechanisms underlying transcriptional regulation of chicken and mammalian IFN-${\gamma}$ genes may be similar.r.

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Default Bayesian Method for Detecting the Changes in Sequences of Independent Exponential and Poisson Random Variates

  • Jeong, Su-Youn;Son, Young-Sook
    • Communications for Statistical Applications and Methods
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    • v.9 no.1
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    • pp.129-139
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    • 2002
  • Default Bayesian method for detecting the changes in sequences of independent exponential random variates and independent Poisson random variates is considered. Noninformative priors are assumed for all the parameters in both of change models. Default Bayes factors, AIBF, MIBF, FBF, to check whether there is any change or not on each sequence and the posterior probability densities of change at each time point are derived. Theoretical results discussed in this paper are applied to some numerical data.