• 식물병연구
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• 제20권1호
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• pp.31-36
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• 2014

우리나라 수박 재배지에서 발생하고 있는 흰가루병 방제를 위한 아인산칼륨-살균제 교차방제체계를 개발하였다. 2010년 시험에서는, 우리나라에서 수박 흰가루병 방제를 위하여 등록되어 합법적으로 사용 가능하며, 농가에서 사용중인 6종류의 살균제를 이용하여 9가지 처리조합을 구성하여 시험하였다. 그 결과 클로로탈로닐(Chl)-마이클로뷰타닐(My)-페나리몰(Fen)-헥사코나졸(Hex)의 처리가 가장 효과가 우수한 것으로 나타났으나, My, Fen, Hex의 작용 기작이 동일하다는 문제점이 있었다. 따라서 2년차 시험에서는 처리조합을 개선하고 농약사용을 줄이기 위하여 이들 살균제 외에 작용기작이 다르면서도 친환경적인 약제인 아인산칼륨을 이용하여 살균제-아인산칼륨 교차 살포 조합을 선발하기 위한 포장시험을 수행하였다. 10간격처리에서, Chl-My-Fen-P는 31.8%의 방제효과를 나타낸 반면, Chl-My-Fen-P는 75.6%의 비교적 높은 방제효과를 나타냈다. 방제효과를 개선하기 위하여 3년차 시험에서는 살균제와 아인산칼륨을 7일 간격으로 살포하였다. Chl-My-Fen-Hex가 가장 높은 89.6%의 방제효과를 나타냈으나, 65.9%의 방제효과를 나타낸Chl-My-Fen-P와 달리 P-My-Fen-Hex 또한 83.5%의 비교적 높은 방제효과를 나타냄으로써, 수박 흰가루병 방제를 위하여 Chl-My-Fen-Hex의 방제체계는 P-My-Fen-Hex로 대체할 수 있음을 시사하고 있다.

• 한국발생생물학회지:발생과생식
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• 제22권3호
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• pp.225-234
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• 2018

A new embryonic cell line (OFEC-17FEN) derived from olive flounder Paralichthys olivaceus was developed. OFEC-17FEN cells were subcultured for <30 passages over ~200 days. OFEC-17FEN cells had a doubling time of 114.34 h and modal diploid chromosome number was 48. The pluripotency genes POU5f1 and NANOG were expressed in OFEC-17FEN cells. However, the lack of several pluripotency-related genes expression indicates that OFEC-17FEN cells are not stem cells. OFEC-17FEN cells transfected with plasmid pEGFP-c1 exhibited a strong green fluorescent signal at 48 h after transfection. Accordingly, OFEC-17FEN cells may be useful for both basic research and biotechnological application.

• 생명과학회지
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• 제20권9호
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• pp.1332-1338
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• 2010

사람 신경모세포종 세포주 SH-SY5Y에서 Fenretinide (FenR)에 의한 GD3합성효소(hST8Sia I)의 발현증가기작을 규명하게 위하여 hST8Sia I의 프로모터 활성을 조사해 본 결과 -1146에서 -646영역에서 FenR에 의한 활성증가를 나타내었다. 또한 부위특이적 변이의 분석은 -731에서 -722영역에 위치한 전사인자 NF-kB 결합부위가 hST8Sia I의 FenR에 의한 활성증가에 중요하게 관여하고 있음을 나타내었다. FenR에 의한 hST8Sia I 유전자의 발현유도에 포함된 신호전달기작을 전사인자 단백질의 항체를 이용하여 조사해 본 결과 FenR처리에 의해 세포질에서는 인산화된 AKT단백질 수준의 증가가 관찰되었고 핵내에서는 NF-kB의 p65단백질의 증가가 관찰되었다. 이러한 결과들은 FenR에 의한 hST8Sia I 유전자의 발현증가는 AKT신호전달경로에 의해 활성화된 NF-kB의 핵내로 이동하여 hST8Sia I 유전자의 프로모터에 결합함으로서 전사가 촉진되어 일어난다는 것을 나타낸다.

• Journal of Microbiology and Biotechnology
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• 제25권10호
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• pp.1768-1771
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• 2015

Human Fen1 protein (hFen1) plays an important role in Okazaki fragment processing by cleaving the flap structure at the junction between single-stranded (ss) DNA and doublestranded (ds) DNA, an intermediate formed during Okazaki fragment processing, resulting in ligatable nicked dsDNA. It was reported that hChlR1, a member of the cohesion establishment factor family, stimulates hFen1 nuclease activity regardless of its ATPase activity. In this study, we found that cohesion establishment factors cooperatively stimulate endonuclease activity of hFen1 in in vivo mimic condition, including replication protein-A-coated DNA and high salt. Our findings are helpful to explain how a DNA replication machinery larger than the cohesion complex goes through the cohesin ring structure on DNA during S phase in the cell cycle.

• 건축역사연구
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• 제31권3호
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• pp.7-16
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• 2022

Fen(分°) is the proportional dimension unit of the standard timber section on Yingzaofashi(營造法式), and there is a phrase that not only structural members but the whole structural design of a building also use Fen as the dimension unit on the book. But in fact only the section dimensions of structural members are recorded by Fen, but the design dimensions are recorded by Chi(尺) on the book. Other historical records also described the building size by Chi. So there has been long-standing debate on the phase in Chinese architectural history society, including the recent confrontation on the analysis of survey figures of the east great hall of Foguangsi temple(佛光寺 東大殿). This paper analyzes all the records about the size of structural members and section planning on the book to make various calculation and evaluation. And it makes a survey of Cai(材) as the dimension and design unit between Chi and Fen through geometric analysis. Cai might be a rough unit of measurement in terms of structural and proportional scheming on Yingzaofashi, and the full size Cai(足材) had been a building scheming module before the Song dynasty.

• 철학연구
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• 제141권
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• pp.167-185
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• 2017

주희(朱熹)는 정이가 제기한 '리일분수(理一分殊)'의 명제를 계승하여 십 수년의 노력을 기울인 후에 최종적으로 "서명해(西銘解)"를 지었다. 이로부터 '리일분수(理一分殊)'의 사상은 "서명(西銘)"을 해석하는 모델이 되었으며, "서명(西銘)"의 기조(基調)를 확정하는 역할을 하게 되었다. '리일분수론(理一分殊論)'은 처음에는 "서명(西銘)"의 윤리학적 의의를 표현하는 명제였다. 그런데 주희(朱熹)의 일생을 통해 볼 때 '리일분수론(理一分殊論)'은 단순히 "서명(西銘)"의 윤리학적 의의에 국한되지 않고, 보편적인 철학적 의의를 포함하며, 사물의 일반과 특수의 관계를 표현하는 데에도 사용되었다. 전자는 협의의 '리일분수론(理一分殊論)'이고, 후자는 광의의 '리일분수론(理一分殊論)'이라고 할 수 있다. 이 글에서는 광의적인 리일분수론(理一分殊論)에 대해서는 논하지 않고, 주희(朱熹)가 확립한 "서명(西銘)"을 해석하는 모델로서의 협의의 리일분수론(理一分殊論)을 연구 대상으로 삼고자 한다. 학술계의 선행연구 중에서 어떤 학자들은 '리일분수(理一分殊)'의 명제가 "서명(西銘)"의 의미와 일치한다고 생각하지만, 다른 어떤 학자들은 일치하지 않는다고 주장한다. 이러한 두 종류의 사고방식은 확연히 상반되는 입장이다. 이 글에서는 리일분수(理一分殊) 사상에 의한 주희(朱熹)의 "서명(西銘)" 해석이 어떤 부분에서는 "서명(西銘)" 본래의 뜻과 일치하기도 하지만, 또다른 어떤 부분에서는 서로 부합하지 않는 곳도 있다는 입장을 견지한다. 달리 말하면, 주희(朱熹)가 리일분수론(理一分殊論)으로 "서명(西銘)"을 해석할 때, '공헌(貢獻)'과 '제한('制限)'의 의미를 동시에 지닌다는 것이다. 즉, 리일분수론(理一分殊論)에 의한 주희(朱熹)의 "서명(西銘)" 해석은 그 의미를 확장시킨 부분도 있지만, "서명(西銘)" 본래의 목표로부터 멀어진 부분도 있다는 의미이다.

• Bulletin of the Korean Chemical Society
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• 제35권10호
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• pp.3005-3008
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• 2014

Human ChlR1 protein (hChlR1), a member of the cohesion establishment factor family, plays an important role in the segregation of sister chromatids for maintenance of genome integrity. We previously reported that hChlR1 interacts with hFen1 and stimulates its nuclease activity on the flap-structured DNA substrate covered with RPA. To elucidate the relationship between hChlR1 and Okazaki fragment processing, the effect of hChlR1 on in vitro nuclease activities of hFen1 and hDna2 was examined. Independent of ATPase activity, hChlR1 stimulated endonuclease activity of hFen1 but not that of hDna2. Our findings suggest that the acceleration of Okazaki fragment processing near cohesions may aid in reducing the size of the replication machinery, thereby facilitating its entry through the cohesin ring.

• BMB Reports
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• 제36권6호
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• pp.580-585
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• 2003

Subtractive library hybridization was used to isolate the cDNA clones that corresponded to the transcripts that were specifically up-regulated during wheat embryo germination. The clones with numbers 5, 6, 7, 8, 24, and 26 appeared to be more abundant in germinating wheat embryos. Among the isolated clones, we identified four new members of the wheat "germin" gene family. We also identified two novel sequences which exhibited distinct germination up-regulation, and displayed characteristic spatial patterns of expression. One of these, represented by clone pSB10, was principally expressed in the root tissue of germinating embryos. The second was represented by the pSB7 clone and was expressed in both the root and shoot primordia of the embryonic axis, as well as within the coleoptile.

• 한국환경과학회지
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• 제20권3호
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• pp.341-350
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• 2011

For the reconstruction of the past vegetational changes in Jeju Island, Korea, pollen analysis and radiocarbon dating on the sediments obtained from the Mulyeongari fen were carried out. By the results, the vegetational changes around the Mulyeongari fen from ca. 3,300 cal. yr BP to the present can be interpreted and reconstructed. The pollen record from the Mulyeongari fen was divided into two local pollen assemblage zones and three sub-pollen assemblage zones. Zone I (Quercus-Carpinus-Herbs Zone) was characterized by the predominance of Quercus(30~63%), Carpinus(9~35%) and herbs(40~424%). Zone II was characterized by three sub-pollen assemblage zones and the high occurrence ratio of the tree layer in comparison with Zone I. In Zone IIa (Quercus-Carpinus Zone), herbs(3~161%) were drastically decreased in predominance of Quercus(28~56%) and Carpinus(14~31%). In Zone IIb (Carpinus-Quercus Zone), Carpinus(36~48%) was preferentially increased but Quercus(29~39%) was slightly decreased. In Zone IIc (Quercus-Carpinus Zone), Carpinus(26~38%) was decreased inversely but Quercus(36~50%) was increased. In addition, Cyperaceae was also increased to 52%. Consequently, it is suggested that cool temperate southern/sub-montane vegetation composed of Quercus and Carpinus which was physiognomy of deciduous broad leaved forest was distributed around the Mulyeongari fen from ca. 3,300 cal. yr BP. In addition, Cyclobalanopsis(4~23%), Castanopsis(1~12%) and Myrica(under 1%) which are warm-temperate evergreen deciduous forest components were constantly appeared from this period. Accordingly, it can be inferred that the present vegetation type around the Mulyeongari fen was formed from ca. 180 cal. yr BP.

• 대한수학회지
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• 제43권4호
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• pp.803-813
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• 2006

We define and investigate a subclass of complex valued harmonic convex functions that are univalent and sense preserving in the open unit disk. We obtain coefficient conditions, extreme points, distortion bounds, convolution conditions for the above family of harmonic functions.