• Title/Summary/Keyword: filter paper test

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Development of a Rapid PCR Test for Identification of Streptococcus agalactiae in Milk Samples Collected on Filter Paper Disks

  • Wu, Jiusheng;Liu, Yuehuan;Hu, Songhua;Zhou, Jiyong
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.1
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    • pp.124-130
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    • 2008
  • Streptococcus (Strep.) agalactiae is one of the major pathogens of bovine mastitis and is the main cause of subclinical infection. This study attempted to develop a rapid PCR diagnosis procedure using milk samples collected on filter paper disks. Chromatographic filter paper was employed as the preservation media and kept at room temperature for one to four weeks. The revival rate of Strep. agalactiae kept on dried filter paper disks was affected by the pretreatment preservation time. The revival test suggested that not all the bacteria in artificially contaminated milk samples on the filter paper disks could be recovered. After that, a PCR based on the 16-23S intergenic spacer region of Strep agalactiae was performed. The results distinguished the strep. agalactiae from major pathogens of bovine mastitis at a $2{\times}10^2$ colony forming units (CFU)/ml level, which showed similar sensitivity to the results from liquid milk samples. The results also showed that milk samples collected on filter paper disks could be kept at room temperature for one to four weeks with little negative effect on sensitivity and specificity. The field test showed that the diagnostic sensitivity and specificity was 96.15% and 98.60%, respectively. In conclusion, the protocol will provide a rapid and economic procedure for the detection of bovine mastitis.

Aircraft parameter estimation using the extended kalman filter (확장 칼만 필터를 이용한 항공기 파라미터 추정)

  • 송용규;황명신;박욱제
    • 제어로봇시스템학회:학술대회논문집
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    • 1997.10a
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    • pp.1655-1658
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    • 1997
  • To obtain aircraft dynamic parameters, various estimation methods such as Maximum Likelihood, Linear Regression are applied. In this paper we adopt the extended Kalman filter(EKF) to estimate the stability and control derivatives in aircraft dynamic models from flight test data. The extended Kalman filter is applied to nonlinear augmented system assuming that unknown parameters are additional states. In this work, the results of the extended Kalman filter are compared with the results of the wind tunnel test using Chang Gong-91 aircraft flight test data.

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Study on bio-degradation of cigarette filter rods with filter materials (필터소재에 따른 담배필터의 생분해성 비교)

  • Ko Dongkyun;Kim Soo-Ho;Shin Chang-Ho;Lee Young-Taek;Kim Chung Ryul;Kim Jong-Yeol
    • Journal of the Korean Society of Tobacco Science
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    • v.27 no.1 s.53
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    • pp.75-82
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    • 2005
  • This work investigated biodegradability for the cellulose acetate, carbon dual, paper and web used to cigarette filter materials by soil test. Also, because of demanded a lot of the time and effort in case of soil test, the possibility of biodegradation by enzyme was studied. The evaluation of degradation for the filter materials by soil test was examined with the naked eye, electron microscopy and weight loss. The biodegradability according to the filter materials was represented in the order of paper > web > carbon dual > cellulose acetate without relating to the evaluation methods. Experiment of biodegradability by the cellulase(E C 3.2.1.4, Trichoderma viride) among the several biodegradability enzymes was demanded reaction time of the $5\~10$ hours and represented the same result with that of soil test.

A Study on the Filter Media and Performance of Intake Air Filter for Vehicular Engine (자동차 흡기 에어필터의 여재 및 성능에 관한 연구)

  • 안병찬;오명도
    • Korean Journal of Air-Conditioning and Refrigeration Engineering
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    • v.16 no.1
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    • pp.8-15
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    • 2004
  • Since the vehicle is regarded as the third living space, the comfortable conditions are required in the passenger compartment. For this reason, customers are concerned about the filtering performances and the importance of filter media has been greatly placed. Therefore the dust holding capacity, the efficiency of these filter media (dry paper, wet paper, non-woven) and the configuration of air filter for vehicle were measured in this study. The following results were obtained on the basis of air filter test. It shows that the thickness and poresize of filter media should be lower for the higher efficiency. The measurement result shows that the performance of round shape filters are higher than the square shape filters. The dust holding capacity of the wet paper and the non-woven paper is higher than the dry paper. As a result, this research can provide an important design parameter and product guidance of the intake air filter for vehicular engine.

Design of Multi-Sensor Data Fusion Filter for a Flight Test System (비행시험시스템용 다중센서 자료융합필터 설계)

  • Lee, Yong-Jae;Lee, Ja-Sung
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.55 no.9
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    • pp.414-419
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    • 2006
  • This paper presents a design of a multi-sensor data fusion filter for a Flight Test System. The multi-sensor data consist of positional information of the target from radars and a telemetry system. The data fusion filter has a structure of a federated Kalman filter and is based on the Singer dynamic target model. It consists of dedicated local filter for each sensor, generally operating in parallel, plus a master fusion filter. A fault detection and correction algorithms are included in the local filter for treating bad measurements and sensor faults. The data fusion is carried out in the fusion filter by using maximum likelihood estimation algorithm. The performance of the designed fusion filter is verified by using both simulation data and real data.

Effect of Sonication on Permeability of Filter Paper (필터재 투과성 효율 증대를 위한 초음파의 활용)

  • 오세헌;황명기;김형수;김영욱
    • Proceedings of the Korean Geotechical Society Conference
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    • 2003.03a
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    • pp.647-654
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    • 2003
  • This study investigated the effect of ultrasound on the permeability of the filter paper The investigation involves laboratory experiments, and the laboratory tests were conducted under abroad range of conditions Including energy levels of ultrasonic waves, time for treatment, and temperature. The results of the study show that sonication enhances the permeability of the filter paper significantly. The degree of enhancement varies with sonication power and duration of application. The effect of sonication on permeability with temperature variation and concentration of the test specimens seems not to be significant.

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Determination of relevant Characteristics for Cartridge Filters

  • Ogawa, Takao
    • Proceedings of the Membrane Society of Korea Conference
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    • 1993.04a
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    • pp.1-11
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    • 1993
  • Cartridge filters are widely used in liquid-solid separation processes. To select a proper filter system for the requirements of a given application, a knowledge of filter efficiency and dirt capacity is required. The filter manufacturer needs to provide data which are based on test procedures simulating real parameters on a laboratory scale. There does not exist at present a standard for liquid filter testing (with the exception of the Multipass Test to ISO4752 for hydraulic filters). Users have to work with data based on different test procedures. This paper will discuss principles of filter testing, efficiency andd dirt capacity results as a function of test parameters, and data interpretion to provide a guideline for the end user.

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A Study on Development of a Hearing Impairment Simulator considering Frequency Selectivity and Asymmetrical Auditory Filter of the Hearing Impaired (난청인의 주파수 선택도와 비대칭적 청각 필터를 고려한 난청 시뮬레이터 개발에 관한 연구)

  • Joo, Sang-Ick;Kang, Hyun-Deok;Song, Young-Rok;Lee, Sang-Min
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.59 no.4
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    • pp.831-840
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    • 2010
  • In this paper, we propose a hearing impairment simulator considering reduced frequency selectivity and asymmetrical auditory filter of the hearing impaired, and we verified the reduced frequency selectivity and asymmetrical auditory filter affected in speech perception through experiments. The reduced frequency selectivity has made embodied by spectral smearing using LPC(linear prediction coding). The shapes of auditory filter are asymmetrical different with each center frequency. Hearing impaired person which has hearing loss was differently changed with that of normal hearing people and it has different value for speech of quality through auditory filter. The experiments confirmed subjective test and objective test. The subjective experiments are composed of 4 kinds of tests: pure tone test, SRT(speech reception threshold) test, and WRS(word recognition score) test without spectral smearing, and WRS test with spectral smearing. The experiment of the hearing impairment simulator was performed from 9 subjects who have normal ears. The amount of spectral smearing was controlled by LPC order. The asymmetrical auditory filter of proposed hearing impairment simulator was simulated and then some tests to estimate the filter's performance objectively were performed. The objective experiment as simulated auditory filter's performance evaluation method used PESQ(perceptual evaluation of speech quality) and LLR(log likelihood ratio) for speech through auditory filter. The processed speech was evaluated objective speech quality and distortion using PESQ and LLR value. When hearing loss processed, PESQ and LLR value have big difference according to asymmetrical auditory filter in hearing impairment simulator.

Studies on the Insecticide Resistance of the German Cockroach(Blattella germanica L.). III. Comparison of Esterase Activity (바퀴(Blattella germanica L.)의 살충제 저항성에 관한 연구. 3. Esterase활성비교)

  • 방종렬;김정화;이형래
    • Korean journal of applied entomology
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    • v.32 no.3
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    • pp.265-270
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    • 1993
  • The German cockroach(Blattelia germanica) population~ were successIVely selected with ch\orpyrifos and permethrin during the six generations. The resulting resistant $R_{chtorpenfos}$(Rc) and $R_{permethnn}$(Rp) stra.ins were studied to investigate the esterase activity by spectrophotometer, filter parper test, and electrophoresis. Esterase-$\alpha$ activities by filter paper test showed 2.65 and LBZ times higher in the Rc and Rp strains than the susceptible strain, respectively. ln the spectrophoLometer method, the esterase activit18s to $\alpha$-and $\beta$-naphthyl acetate were increased 2.34 and 5.28 times in the Rc than susceptible strain, and 1.48 and 2.92 times in the Rp Limn susceptible stram, respectlvely. Zymogram patterns of eslerase isozyme by agarose gel electrophoresis showed totally five bands. The Rc and Rp strains showed two additive bands as, Est-2 and Est-3, which were not shown in the susceptible strain. but the Rp strain dId not show Est-5 bands which was COlumon in the Rc and susceptible strams.

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Evaluation of Hydraulic Conductivity of Bentonite Filter Cake Using Modified Fluid Loss Test

  • Nguyen, The Bao;Lee, Chul-Ho;Yang, Jung-Hun;Choi, Hang-Seok
    • Proceedings of the Korean Geotechical Society Conference
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    • 2008.10a
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    • pp.498-507
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    • 2008
  • The mixture of bentonite powder and water is generally used to maintain the stability of excavation surface during the construction of vertical cutoff walls. The filter cake on the sidewall surface is the result of filtration of slurry into the adjacent soil formation. The filter cake is believed to have a very low hydraulic conductivity compared to that of the cutoff wall. This paper evaluates hydraulic conductivities of bentonite filter cakes set up with three types of bentonites under various pressure levels. A modified fluid loss test was employed in this experiment. Theory of filtration process was reviewed to explain the procedure in the present experiment. Hydraulic conductivity of the filter cakes with consideration of the filter medium resistance was evaluated. The results of the experiment with two calculation methods and discussion are presented to show the efficiency of the modified fluid loss test.

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